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GARİP, GÜNSELİ AYŞE

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GARİP

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GÜNSELİ AYŞE

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2010 - 20192
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End date: 2019 × Subject: APOPTOSIS ×

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    PublicationOpen Access
    Synergistic Effects of 4-Aminopyridine and Paclitaxel on MCF 7 Cell Line
    (MARMARA UNIV, INST HEALTH SCIENCES, 2019-09-15) GARİP, GÜNSELİ AYŞE; Aydogmus, Esra M. Cuce; Garip, Gunseli Ayse Inhan
    Objectives: Aim of this study is to increase the effectiveness of paclitaxel (PTX) with the use of 4 - aminopyridine (4-AP) on breast cancer cell line MCF-7. Methods: In this study, L-929 (ATCC CRL-6364) and MCF-7 (ATCC - HTB 22) cell lines were used. IC50 and survival values were determined by trypan blue exclusion; cell cycle analysis was determined by measuring levels of Cdk2 and Histone (H3) and plasma membrane potential (Vm) measurements were performed using fluorescent Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBaC4(3)). Results: IC50 values were determined for two agents and these values were combined. Combination treatments ie. 4-AP (4 mM) + PTX (5 nM) and 4-AP (4 mM) + PTX (7.5 nM) decreased viability 17% +/- 8.08 and 45% +/- 3.18, respectively for L-929 cells and decreased viability 60%+/- 3.7 and 74%+/- 2.6, respectively for MCF-7 cells. For L-929 cells, plasma membrane potential measurements resulted in depolarization for 4-AP, PTX (5 nM) and PTX (7.5 nM), and resulted in hyperpolarization for the combinations. For MCF-7 cells, plasma membrane potential measurements resulted in depolarization for 4-AP, PTX (7.5 nM) and 4-AP + PTX (5 nM), and resulted in hyperpolarization for PTX (5 nM) and 4-AP + PTX (7.5 nM). Changes of Cdk2 and H3 levels showed mostly G1 arrest for MCF-7 cells and G2/M arrest for L-929 cells. Conclusions: Combination treatments increased the cell death for MCF-7 cells. But, combination treatments didn't show synergistic effect on L-929 which is accepted as a non-cancerous cell. These data showed that use of 4-AP in combination with the anticancer agent paclitaxel is a promising approach for cancer treatment.
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    Extremely Low-Frequency Electromagnetic Fields Affect the Immune Response of Monocyte-Derived Macrophages to Pathogens
    (WILEY, 2010) GARİP, GÜNSELİ AYŞE; Akan, Zafer; Aksu, Burak; Tulunay, Aysin; Bilsel, Serpil; Inhan-Garip, Ayse
    This study aimed to determine the effect of extremely low-frequency electromagnetic fields (ELF-EMF) on the physiological response of phagocytes to an infectious agent. THP-1 cells (human monocytic leukemia cell line) were cultured and 50 Hz, 1 mT EMF was applied for 4-6 h to cells induced with Staphylococcus aureus or interferon gamma/lipopolysaccharide (IF gamma/LPS). Alterations in nitric oxide (NO), inducible nitric oxide synthase (iNOS) levels, heat shock protein 70 levels (hsp70), cGMP levels, caspase-9 activation, and the growth rate of S. aureus were determined. The growth curve of exposed bacteria was lower than the control. Field application increased NO levels. The increase was more prominent for S. aureus-induced cells and appeared earlier than the increase in cells without field application. However, a slight decrease was observed in iNOS levels. Increased cGMP levels in response to field application were closely correlated with increased NO levels. ELF-EMF alone caused increased hsp70 levels in a time-dependent manner. When cells were induced with S. aureus or IF gamma/LPS, field application produced higher levels of hsp70. ELF-EMF suppressed caspase-9 activation by a small extent. These data confirm that ELF-EMF affects bacterial growth and the response of the immune system to bacterial challenges, suggesting that ELF-EMF could be exploited for beneficial uses. Bioelectromagnetics 31:603-612, 2010. (C) 2010 Wiley-Liss, Inc.