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GARİP, GÜNSELİ AYŞE

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GARİP

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GÜNSELİ AYŞE

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2010 - 20112
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End date: 2023 × Subject: STAPHYLOCOCCUS-AUREUS ×

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    Effect of extremely low frequency electromagnetic fields on growth rate and morphology of bacteria
    (TAYLOR & FRANCIS LTD, 2011) ÖZAYDIN, AYŞE NİLÜFER; Inhan-Garip, Ayse; Aksu, Burak; Akan, Zafer; Akakin, Dilek; Ozaydin, A. Nilufer; San, Tangul
    Purpose: To determine the effect of extremely low frequency (<300 Hz) electromagnetic fields (ELF-EMF) on the growth rate of Gram-positive and Gram-negative bacteria and to determine any morphological changes that might have been caused by ELF-EMF. Materials and methods: Six bacterial strains, three Gram-negative and three Gram-positive were subjected to 50 Hz, 0.5 mT ELF-EMF for 6 h. To determine growth rate after ELF-EMF application, bacteria exposed to ELF-EMF for 3 h were collected, transferred to fresh medium and cultured without field application for another 4 h. Growth-rate was determined by optical density (OD) measurements made every hour. Morphological changes were determined with Transmission electron microscopy (TEM) for two gram-negative and two gram-positive strains collected after 3 h of field application. Results: A decrease in growth rate with respect to control samples was observed for all strains during ELF-EMF application. The decrease in growth-rate continued when exposed bacteria were cultured without field application. Significant ultrastructural changes were observed in all bacterial strains, which were seen to resemble the alterations caused by cationic peptides. Conclusion: This study shows that ELF-EMF induces a decrease in growth rate and morphological changes for both Gram-negative and Gram-positive bacteria.
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    Extremely Low-Frequency Electromagnetic Fields Affect the Immune Response of Monocyte-Derived Macrophages to Pathogens
    (WILEY, 2010) GARİP, GÜNSELİ AYŞE; Akan, Zafer; Aksu, Burak; Tulunay, Aysin; Bilsel, Serpil; Inhan-Garip, Ayse
    This study aimed to determine the effect of extremely low-frequency electromagnetic fields (ELF-EMF) on the physiological response of phagocytes to an infectious agent. THP-1 cells (human monocytic leukemia cell line) were cultured and 50 Hz, 1 mT EMF was applied for 4-6 h to cells induced with Staphylococcus aureus or interferon gamma/lipopolysaccharide (IF gamma/LPS). Alterations in nitric oxide (NO), inducible nitric oxide synthase (iNOS) levels, heat shock protein 70 levels (hsp70), cGMP levels, caspase-9 activation, and the growth rate of S. aureus were determined. The growth curve of exposed bacteria was lower than the control. Field application increased NO levels. The increase was more prominent for S. aureus-induced cells and appeared earlier than the increase in cells without field application. However, a slight decrease was observed in iNOS levels. Increased cGMP levels in response to field application were closely correlated with increased NO levels. ELF-EMF alone caused increased hsp70 levels in a time-dependent manner. When cells were induced with S. aureus or IF gamma/LPS, field application produced higher levels of hsp70. ELF-EMF suppressed caspase-9 activation by a small extent. These data confirm that ELF-EMF affects bacterial growth and the response of the immune system to bacterial challenges, suggesting that ELF-EMF could be exploited for beneficial uses. Bioelectromagnetics 31:603-612, 2010. (C) 2010 Wiley-Liss, Inc.