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GARİP, GÜNSELİ AYŞE

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GARİP

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GÜNSELİ AYŞE

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2007 - 20102
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Subject: K562 ×

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    Effect of ELF-EMF on K562 cell differentiation in the presence or absence of quercetin and heat-shock
    (TAYLOR & FRANCIS LTD, 2007) GARİP, GÜNSELİ AYŞE; Garip-Inhan, A.; Isal-Tugut, I.; Kalkan, M. T.
    Extremely low frequency electromagnetic fields (ELF-EMF) are known to produce a variety of biological effects although the underlying mechanism has not been elucidated yet. The present study aimed to investigate the effect of ELF-EMF on K562 cells induced to differentiate with or without quercetin with application of heat-shock. 50Hz ELF-EW was applied at three (1mT,5mT,10mT) field intensities. Quercetin is,flavonoid known to have anti-tumorigenic and anti-proliferative properties. Heat-shock was applied since it has been shown that quercetin down-regulates but ELF-EMF increases heat-shock protein levels. Results of this study show that ELF-EMF increases differentiation of untreated K562 cells at low field intensities in accordance with the window effect of these fields. Heat-shock abolishes this increase. Heat-shock also decreases the %differentiation of quercetin induced cells. However it has no effect on quercetin induced ELF-EMF exposed cells implying a protective effect of ELF-EMF These results prompted us to hypothesize that these fields can act antagonistically with heat-shock under certain conditions.
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    Differentiation of K562 Cells Under ELF-EMF Applied at Different Time Courses
    (TAYLOR & FRANCIS INC, 2010) GARİP, GÜNSELİ AYŞE; Ayse, Inhan-Garip; Zafer, Akan; Sule, Oncul; Isil, Isal-Turgut; Kalkan, Tunaya
    The time-course of ELF-EMF application to biological systems is thought to be an important parameter determining the physiological outcome. This study investigated the effect of ELF-EMF on the differentiation of K562 cells at different time courses. ELF-EMF (50 Hz, 5 mT, 1 h) was applied at two different time-courses; first at the onset of hemin induction for 1 h, and second, daily 1 h for four days. While single exposure to ELF-EMF resulted in a decrease in differentiation, ELF-EMF applied everyday for 1 h caused an increase in differentiation. The effect of co-stressors, magnesium, and heat-shock was also determined and similar results were obtained. ELF-EMF increased ROS levels in K562 cells not treated with hemin, however did not change ROS levels of hemin treated cells indicating that ROS was not the cause. Overall, these results imply that the time-course of application is an important parameter determining the physiological response of cells to ELF-EMF.