Person: ÖZDEMİR KUMRAL, ZARİFE NİGAR
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ÖZDEMİR KUMRAL
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ZARİFE NİGAR
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Publication Open Access The potency of obestatin in improving kidney functions and apoptosis in rats with cisplatin-induced acute kidney injury(2022-01-01) ÖZDEMİR KUMRAL, ZARİFE NİGAR; BULUT, ALİSİNA; ÖZKAN YENAL, NAZİYE; YEGEN, BERRAK; KOÇ, MEHMET; ÖZDEMİR KUMRAL Z. N. , BULUT A., Üzülmez B., Vezirhüyük M., Kök Z., ÖZKAN YENAL N., YEGEN B., KOÇ M.© 2022 Marmara University Press.Cisplatin (CP), which is the most commonly used anticancer agent to treat several solid tumors, may cause acute kidney injury (AKI) as the major limiting factor for its clinical use. Obestatin (OB) is a ghrelin gene-derived peptide produced in several tissues and has shown anti-oxidant, anti-apoptotic, and anti-inflammatory effects in many experimental models. This study investigated the effect of OB treatment on nephrotoxicity induced by CP. Rats were divided into 4 groups as two control (1 ml/kg, saline, intraperitoneal (ip), single dose) and two CP-induced (7 mg/kg, ip, single dose) AKI groups (8 rats in each group). Immediately after the CP injection and the following two days, injections of OB (10 µg/kg, ip) were performed. Rats were decapitated at the end of 72 hours. Blood and kidney tissue samples were taken for biochemical and histopathological measurements. The results of the present study revealed that serum creatinine and BUN levels were significantly increased in the CP-induced AKI group when compared to the control group. Treatment with OB improved kidney functions and ameliorated renal oxidative injury and maintained oxidative balance in the CP-induced AKI model, which was revealed by elevated malondialdehyde and depleted glutathione levels. TUNEL scores also demonstrated that CP increased the apoptotic response, while OB treatment abolished it. CP-induced medullary and cortical injuries were also partially reversed by OB treatment. Thus, our findings show that OB alleviates CP-induced nephrotoxicity in rats through the abolishment of oxidative stress and apoptosis.Publication Metadata only Phoenixin-14 Sepsise Bağlı Gelişen Kardiyopulmoner Hasarı Hafifletir: Vagal Tonusun Rolü(2021-10-10) ÖZDEMİR KUMRAL, ZARİFE NİGAR; UPRAK, TEVFİK KIVILCIM; YEGEN, BERRAK; ÖZDEMİR KUMRAL Z. N., ŞEN L. S., Çantalı Öztürk Ç., UPRAK T. K., YEGEN B.AMAÇ: Sepsis, sistemik inflamasyon ve yaşamsal organların disfonksiyonu ile tanımlanan yıkıcı bir hastalıktır. Phoenixin’in (PNX-14) çoğunlukla hipotalamusta ve kalpte eksprese olduğu ve iskemiyi takiben kardiyoproteksiyon sağladığı gösterilmiştir. Çalışmanın amacı, PNX-14'ün sepsis kaynaklı kardiyopulmoner hasar üzerinde yararlı etkisinin olup olmadığını değerlendirmek ve vagal liflerin bu etkiye katkısını açıklamaktır. YÖNTEMLER: Ketamin anestezisi altında, erkek SpragueDawley sıçanlara (310-390 g) taklit cerrahi (n=8) veya sepsisi indüklemek için çekal ligasyon ve ponksiyon (n=23) yapıldı. Sepsis yapılmadan önce, serum fizyolojik veya PNX-14 (50 µg/kg/gün) son 36 saat içinde tekrarlayan 3 doz şeklinde intraperitoneal olarak uygulandı. PNX-14 tedavisi alan sıçanların yarısında ek olarak vagal stimülasyon (VS) yapılırken diğerlerine VS yapılmadı. VS amacıyla sıçanlara, sepsisten 7 gün önce başlayarak, diazepam (5mg/kg) anestezisi altında aurikula konkalarındaki elektrotlar aracılığıyla düşük seviyeli transkutanöz VS (20 Hz, 0,2ms, 2mA, 30 dk/gün) uygulandı. Sepsisin 16. saatinde serum TNF-α ve IL-6 düzeylerini ölçmek için kardiyak ponksiyonla kan alındı, histolojik değerlendirme ve miyeloperoksidaz aktivitesi, glutatyon, katalaz, süperoksit dismutaz (SOD), malondialdehit seviyelerinin belirlenmesi için kalp ve akciğer örnekleri alındı. Veriler Student t-testi ve tek-yönlü ANOVA kullanılarak analiz edildi. BULGULAR: Sepsis, her iki dokuda hipotermiye, serum TNF-α ve IL-6 seviyelerinde artışa, her iki dokuda miyeloperoksidaz aktivitesinde ve histolojik hasar skorlarında artışa neden olurken, katalaz ve glutatyon seviyelerinde azalmaya neden oldu (p<0,05-0,001). Ayrıca, septik sıçanların kalp dokularında malondialdehit ve SOD seviyelerinin de yükseldiği gözlendi (p<0,05). PNX-14 hipotermiyi, her iki dokunun miyeloperoksidaz aktivitesini ve hasar skorlarını azaltırken, kalp dokusunda malondialdehit seviyesini düşürdü. Ayrıca, PNX ile kardiyak glutatyon, katalaz düzeyleri ve akciğerde SOD seviyeleri artmış bulundu (p<0,05-0,001). PNX tedavisi ile her iki dokunun histolojik skorlarında ve kalp dokusu malondialdehit seviyesindeki gözlenen azalmalar ile kalp dokusunun glutatyon ve katalaz düzeylerinde ve akciğerin SOD seviyelerindeki artışlar VS ile tersine çevrildi. SONUÇ: PNX-14, sepsise bağlı kalp ve akciğerde gözlenen oksidatif hasarı iyileştirmektedir ve bunu kısmen vagal tonus üzerindeki inhibitör etkiyle gerçekleştirmektedir.Publication Open Access The gastroprotective effect of obestatin on indomethacin-induced acute ulcer is mediated by a vagovagal mechanism(AKADEMIAI KIADO ZRT, 2020-06) YEGEN, BERRAK; Sen, Leyla Semiha; Kumral, Zarife Nigar Ozdemir; Memi, Gulsun; Ercan, Feriha; Yegen, Berrak C.; Yegen, CumhurIn order to investigate the role of the vagus nerve in the possible gastroprotective effect of obestatin on the indomethacin-induced acute oxidative gastric injury, Sprague-Dawley rats of both sexes were injected subcutaneously with indomethacin (25 mg/kg, 5% NaHCO3) followed by obestatin (10, 30 or 100 mu g/kg). In other sets of rats, surgical vagotomy (Vx) or selective degeneration of vagal afferent fibers by perivagal capsaicin was performed before the injections of indomethacin or indomethacin + obestatin (30 mu g/kg). Gastric serosal blood flow was measured, and 4 h after ulcer induction gastric tissue samples were taken for histological and biochemical assays. Obestatin reduced the severity of indomethacin-induced acute ulcer via the reversal of reactive hyperemia, by inhibiting ulcer-induced neutrophil infiltration and lipid peroxidation along with the replenishment of glutathione (GSH) stores, whereas Vx abolished the inhibitory effect of obestatin on blood flow and lipid peroxidation, and worsened the severity of ulcer. On the other hand, serosal blood flow was even amplified by the selective denervation of the capsaicin-sensitive vagal afferent fibers, but obestatin-induced reduction in ulcer severity was not altered. In conclusion, the gastroprotective effect of obestatin on indomethacin-induced ulcer appears to involve the activation of the vagovagal pathway.Publication Metadata only Phoenixin 14 ameloriates pancreatic injury in streptozotocin-induced diabetic rats by alleviating oxidative burden(2022-09-01) ÖZDEMİR KUMRAL, ZARİFE NİGAR; YÜKSEL, MERAL; AKAKIN, DİLEK; ERZİK, CAN; HAKLAR, GONCAGÜL; ÖZDEMİR KUMRAL Z. N. , Sen E., Yapici H. B. , Atakul N., Domruk O. F. , Aldag Y., Sen L. S. , Mustafaoglu F. K. , YÜKSEL M., AKAKIN D., et al.Phoenixin-14 (PNX) is a neuropeptide that has been shown to prevent oxidative damage and stimulates insulin secretion. We investigated the effects of PNX on pancreatic injury induced by streptozotocin (STZ), and nicotinamide (NAD). Male Sprague-Dawley rats, in control (C) and diabetic (STZ) groups, were treated with either saline, or PNX (0.45 nmol/kg, or 45 nmol/kg) daily for 3 days 1 week after STZ injection. Fasting blood glucose (FBG) and gastric emptying rate (GER) were measured. Tissue and blood samples were collected. PNX treatments prevented pancreatic damage and beta cell loss. Increased luminol and lucigenin levels in the pancreas, ileum and liver tissues of STZ groups were alleviated by PNX treatment in pancreatic and ileal tissues. PNX0.45 decreased FBG without any change in insulin blood level and pancreatic mRNA. GER increased in all diabetic rats while PNX0.45 delayed GER only in the C group. PNX diminishes pancreatic damage and lowers FBG by reducing oxidative load.Publication Metadata only Effects of chronic stress and caffeine consumption on visceral pain and emotional status: the role of adenosine and estrogen receptors(2023-01-01) KAHRAMAN, MERVE MERİÇ; ÖZDEMİR KUMRAL, ZARİFE NİGAR; YEGEN, BERRAK; Uzun S. K., KAHRAMAN M. M., Mermer K. S., ÖZDEMİR KUMRAL Z. N., YEGEN B.