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TOKSOY ÖNER, EBRU

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TOKSOY ÖNER

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2018 - 201912020 - 20211
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Author: GÜNDÜZ, OĞUZHAN × Subject: Halomonas levan ×

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    Levan based fibrous scaffolds electrospun via co-axial and single-needle techniques for tissue engineering applications
    (ELSEVIER SCI LTD, 2018) TOKSOY ÖNER, EBRU; Avsar, Gulben; Agirbasli, Deniz; Agirbasli, Mehmet Ali; Gunduz, Oguzhan; Oner, Ebru Toksoy
    This represents the first systematic study where levan polysaccharide was used to fabricate fibrous matrices by co-axial and single-needle electrospinning techniques. For this, hydrolyzed (hHL) and sulfated hydrolyzed (ShHL) Halomonas levan were chemically synthesized and used together with polycaprolactone (PCL) and polyethyleneoxide (PEO) for the spinning process. In co-axially spun matrices, ultimate tensile strength (UTS) were found to increase with increasing ShHL concentration and elongation at break of PCL + ShHL matrices increased up to ten-fold when compared to PCL matrices. Similarly, in single-needle spun matrices, higher elongation at break values were obtained by blending HL and ShHL with PEO pointing to the effective energy absorbing features. Dense and fine fibers were characterized by FTIR and SEM. Cell viability and fluorescence imaging of L929 fibroblasts and HUVECs as well as heparin mimetic activity of the matrices pointed to their high potential to be used in decreasing neointimal proliferation and thrombogenicity of grafts and prosthesis.
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    PublicationOpen Access
    Resveratrol-Loaded Levan Nanoparticles Produced by Electrohydrodynamic Atomization Technique
    (MDPI, 2021-09-30) TOKSOY ÖNER, EBRU; Cinan, Ezgi; Cesur, Sumeyye; Haskoylu, Merve Erginer; Gunduz, Oguzhan; Oner, Ebru Toksoy
    Considering the significant advances in nanostructured systems in various biomedical applications and the escalating need for levan-based nanoparticles as delivery systems, this study aimed to fabricate levan nanoparticles by the electrohydrodynamic atomization (EHDA) technique. The hydrolyzed derivative of levan polysaccharide from Halomonas smyrnensis halophilic bacteria, hydrolyzed Halomonas levan (hHL), was used. Nanoparticles were obtained by optimizing the EHDA parameters and then they were characterized in terms of morphology, molecular interactions, drug release and cell culture studies. The optimized hHL and resveratrol (RS)-loaded hHL nanoparticles were monodisperse and had smooth surfaces. The particle diameter size of hHL nanoparticles was 82.06 & PLUSMN; 15.33 nm. Additionally, release of RS from the fabricated hHL nanoparticles at different pH conditions were found to follow the first-order release model and hHL with higher RS loading showed a more gradual release. In vitro biocompatibility assay with human dermal fibroblast cell lines was performed and cell behavior on coated surfaces was observed. Nanoparticles were found to be safe for healthy cells. Consequently, the fabricated hHL-based nanoparticle system may have potential use in drug delivery systems for wound healing and tissue engineering applications and surfaces could be coated with these electrosprayed particles to improve cellular interaction.