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TOKSOY ÖNER, EBRU

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TOKSOY ÖNER

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2016 - 20192
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Author: SENNAROĞLU BOSTAN, MÜGE × Subject: DELIVERY ×

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    Lecithin-acrylamido-2-methylpropane sulfonate based crosslinked phospholipid nanoparticles as drug carrier
    (WILEY, 2016) TOKSOY ÖNER, EBRU; Mutlu, Esra Cansever; Bostan, Muge Sennaroglu; Bahadori, Fatemeh; Kocyigit, Abdurrahim; Oner, Ebru Toksoy; Eroglu, Mehmet S.
    In this study, a novel paclitaxel (PTX) loaded and a crosslinked solid phospholipid nanoparticles (SLN-PTX) with negative surface charge was prepared by UV polymerization for drug delivery. Capping of positive charge of zwitterionic lecithin with negative charge of sodium 2-acrylamido-2-methyl-1-propanesulfonate (AMPS-Na) through cation exchange interaction produced a lecithin-AMPS (L-AMPS) complex. The amphiphilic and negative charged lipid complex was emulsified in the presence of emulsifier, paclitaxel, initiator, and methacrylated poly epsilon-caprolacton-diol (PCL-MAC) as a spacer. The colloidal system was subjected to UV-irradiation to obtain crosslinked nanoparticles. Completion of the UV-polymerization was monitored with differential scanning calorimetry (DSC), which indicated the disappearance of exothermic peaks of vinyl groups. The nanoparticle system, having an average size of 200 nm, exhibited high drug encapsulation (96%) with negatively charged surface (zeta potential had an average of -70 mV). PTX release profiles of the crosslinked and uncrosslinked SLN-PTXs were studied and their pharmacological properties were compared. The crosslinked nanoparticles exhibited more controlled release behavior with longer release time compared to the uncrosslinked ones. In vitro cytotoxicity test was conducted on MCF-7 human breast adenocarcinoma cell line, which indicated that the crosslinked SLN-PTXs have a potential therapeutic effect for breast cancer treatments. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 44105.
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    Gradient multifunctional biopolymer thin film assemblies synthesized by combinatorial MAPLE
    (ELSEVIER SCIENCE BV, 2019) TOKSOY ÖNER, EBRU; Mihailescu, Natalia; Haskoylu, Merve Erginer; Ristoscu, Carmen; Bostan, Muge Sennaroglu; Sopronyi, Mihai; Eroglu, Mehmet S.; Chifiriuc, Mariana Carmen; Mustaciosu, Cosmin Catalin; Axente, Emanuel; Oner, Ebru Toksoy; Mihailescu, Ion N.
    Combinatorial Matrix-Assisted Pulsed Laser Evaporation (C-MAPLE) was recently introduced to the fast generation of compositional libraries of two biopolymers in a single-step process, for tissue engineering and regenerative medicine applications. Synchronized laser irradiation of two distinct cryogenic targets, one consisting of Sulfated Halomonas Levan and the other of quaternized low molecular weight Chitosan was used to fabricate compositional gradient coatings for surface functionalization. Synthesized coatings preserved the base material composition as confirmed by Fourier Transform Infrared Spectroscopy. Morphological study by Scanning Electron Microscopy, Atomic Force Microscopy and profilometry correlated with water contact angles measurements demonstrated that the obtained thin coatings have improved surface properties with respect to pure material coatings. Fluorescence microscopy validated the compositional gradient, while in vitro assays evidenced characteristic responses of mouse fibroblasts (L929 cell line) by distinct deposition surface regions. The coagulation test pointed out good properties for Sulfated Halomonas Levan coatings as compared to the case of an increased amount of quaternized low molecular weight Chitosan biopolymer or the control. The antimicrobial effect of the coatings was demonstrated against Escherichia coli and Staphylococcus aureus strains, representative for both Gram negative and Gram positive bacterial species, respectively, mainly involved in implant and nosocomial infections. The assembled nanostructures possess variable anti-biofilm activity along the compositional gradient, with a stronger inhibitory effect on the initial adherence phase of both tested microbial strains, but also against mature Escherichia coli biofilms. It was shown that C-MAPLE can generate discrete areas of blended polymeric composition exhibiting improved surface properties for a broad range of biomedicine applications, e.g. the fabrication of thin bioactive and cell-instructive coatings with anti-adherence properties.