Person: BECEREN, AYFER
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BECEREN
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AYFER
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Publication Metadata only Ginkgo biloba extract protects against mercury(II)-induced oxidative tissue damage in rats(PERGAMON-ELSEVIER SCIENCE LTD, 2007) VELİOĞLU ÖĞÜNÇ, AYLİZ; Sener, Goksel; Sehirli, Ozer; Tozan, Ayfer; Velioglu-Ovunc, Ayliz; Gedik, Nursal; Omurtag, Gulden Z.Mercury(II) is a highly toxic metal which induces oxidative stress in the body. In this study we aimed to investigate the possible protective effect of Ginkgo biloba (EGb), an antioxidant agent, against experimental mercury toxicity in rat model. Following a single dose of 5 mg/kg mercuric chloride (HgCl2; Hg group) either saline or EGb (150 mg/kg) was administered for 5 days. After decapitation of the rats trunk blood was obtained and the tissue samples from the brain, lung, liver, and kidney were taken for the determination of malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen contents. Formation of reactive oxygen species in the tissue samples was monitored by chemiluminescence (CL) technique. BUN, creatinin, ALT, and AST levels and tumor necrosis factor-alpha (TNF-alpha) and lactate dehydrogenase (LDH) activity were assayed in serum samples. The results revealed that HgCl2 induced oxidative damage Caused significant decrease in GSH level, significant increase in MDA level, MPO activity and collagen content of the tissues. Treatment of rats with EGb significantly increased the GSH level and decreased the MDA level, MPO activity, and collagen contents. Similarly, serum ALT, AST and BUN levels, as well as LDH and TNF-alpha, were elevated in the Hg group as compared to control group. On the other hand, EGb treatment reversed all these biochemical indices. Our results implicate that mercury-induced oxidative damage in brain, lung, liver, and kidney tissues protected by G. biloba extract, with its antioxidant effects. (c) 2006 Published by Elsevier Ltd.Publication Metadata only Comparison of inhaled and intraperitoneal formaldehyde toxicity in rats and the evaluation of the effects of melatonin(ELSEVIER IRELAND LTD, 2016) BECEREN, AYFER; Aydemir, S.; Akgun, S. G.; Ozkan, N.; Yuksel, M.; Beceren, A.; Erdogan, N.; Omurtag, G. Z.Publication Metadata only Evaluation of incision wound healing activity of Scorzonera veratrifolia in Wistar albino rats(ELSEVIER IRELAND LTD, 2017) BECEREN, AYFER; Gecim, Mert; Beceren, Ayfer; Aydemir, Sezgin; Sardas, SemraPublication Metadata only Evaluation of antioxidant, analgesic, anti-inflammatory and antispasmodic activity and genotoxic effect of micromeria fruticosa subsp brachycalyx in vitro and in vivo(2021-07-22) KABASAKAL, LEVENT; TAŞKIN, TURGUT; AYDEMİR, SEZGİN; BECEREN, AYFER; Çelikkol I., Beceren A., Kabasakal L., Taşkın T., Aydemir S.EVALUATION OF ANTIOXIDANT, ANALGESIC AND ANTIINFLAMMAUTORY ACTIVITY AND GENOTOXIC EFFECT OF MICROMERIA FRUTICOSA SUBSP BRACHYCALYX IN VITRO AND IN VIVO1Celikkol I., 2Beceren A., 3Kabasakal L., 4Taskin T., 5Aydemir S.1Marmara University, Institute of Health Sciences, Department of Pharmaceutical Toxicology Istanbul, Turkey, celikkolisik@gmail.com 2Marmara University, Department of Pharmaceutical Toxicology, Istanbul, Turkey, ayfertozan@hotmail.com 3Marmara University, Department of Pharmacology, Istanbul, Turkey, lkabasakal@gmail.com 4Marmara University, Department of Pharmacognosy, Istanbul, Turkey, turguttaskin@marmara.edu.tr 5Marmara University, Vocational School of Health Services, Department of Medical Services and Techniques, Istanbul, Turkey, szgnaydemir@gmail.comIntroduction: Today, antioxidants have been using diversely in various medical conditions. Therefore, there are growing interest for discovering and developing more effective and safer antioxidants derived from natural sources. Certain Micromeria species were identified as a rich source of antioxidant agents (1). This study aimed to investigate possible antioxidant activity and genotoxicity of Micromeria fruticosa subsp brachycalyx in methanol extract both in vitro and in vivo.Materials and Methods: In vitro antioxidant activity of Micromeria fruticosa subsp brachycalyx in methanol extract was evaluated by DPPH, ABTS, FRAP and CUPRAC assays. In vivo analyses performed on Balb/c mice that divided to three groups (n=6 for each group) as control group (%0,04 Carboxymethyl cellulose solution (CMC), 0,1 mL/10 g, p.o.), positive control group (Indomethacin in %0,04 CMC, 10 mg/kg, p.o.) and treatment group (Micromeria extract in %0,04 CMC, 200 mg/kg, p.o.) and each regimen was applied for 10 days. Myeloperoxidase levels were analyzed in mice liver and kidney tissues. Genotoxic effect was determined in mice blood with Comet Technique. The 8-hydroxy-2’-deoxyguanosine was analyzed in mice liver and kidney tissues with commercial ELISA kit. Results: The results showed that plant extract showed stronger DPPH radical scavenging activity, however, lower ABTS, CUPRAC and FRAP values versus to standards. According to the graph, which shows the elapsed time (second) in pain behavior versus time (minute) for each group, treatment group exhibited its efficacy in late phase (20-30 min) similar with indomethacin group. Myeloperoxidase levels in liver tissues of the extract group were significantly lower compared to the control group (p<0.05). Myeloperoxidase levels in kidney tissues of the extract group were not significantly different compared to both control and indomethacin group. Micromeria fruticosa subsp brachycalyx extract exerted no genotoxic effect on DNA similarly both control and indomethacin group.Conclusions: Our study showed that Micromeria fruticosa subsp brachycalyx has a potential antioxidant activity. It has also been contributed to raise awareness to this species in terms of its safety with the obtained results. Therefore, the usage of this species as natural antioxidant source is promising for new drug candidates.Acknowledgements:Not available.References: 1. Salameh N et al., (2020) Screening of Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia Volatile Oils: A Comparative Study of Plants Collected from Different Regions of West. BioMed Research International, 1-7.Publication Metadata only The wound healing effects of Nerium oleander extract against burn-induced oxidative injury(ELSEVIER IRELAND LTD, 2016) BECEREN, AYFER; Akgun, S. G.; Aydemir, S.; Ozkan, N.; Beceren, A.; Sardas, S.Publication Metadata only Protective role of St. John's Wort on formaldehyde-induced lung tissue injury: Inhibiton of inflammation and oxidative stress mediated apoptosis(ELSEVIER IRELAND LTD, 2017) BECEREN, AYFER; Beceren, Ayfer; Aydemir, Sezgin; Ozakpinar, Ozlem Bingol; Sehirli, Ahmet Ozer; Omurtag, Gulden ZehraPublication Metadata only The role of St. John's Wort against formaldehyde toxicity induced by inhalation in rats(ELSEVIER IRELAND LTD, 2017) BECEREN, AYFER; Aydemir, Sezgin; Beceren, Ayfer; Gecim, Mert; Ozakpinar, Ozlem Bingol; Sehirli, Ahmet Ozer; Omurtag, Gulden ZehraPublication Metadata only Comparision of biocompatibility and cytotoxicity of two new root canal sealers(ELSEVIER GMBH, 2010) BECEREN, AYFER; Gencoglu, Nimet; Sener, Goksel; Omurtag, Gulden Z.; Tozan, Ayfer; Uslu, Bahar; Arbak, Serap; Helvacioglu, DilekThe aim of this study was to investigate the remote organ toxicity and connective tissue reaction of two new root canal sealers (GuttaFlowa(R) and EndoREZ(R)) and to compare them with zinc oxide eugenol sealer using biochemical and histopatho-logical parameters A total of 60 white albino Wistar rats were used in the study 0 1 ml of GuttaFlow(R), EndoREZ(R) or Kerr Pulp Canal Sealer(R) were administered subcutaneously into the mid dorsal thoracic region of rats (15 in each group) Control rats were given saline only Rats were decapitated after 24 h, on day 7 and on day 30 of the experiment and tissue samples from lung, liver, kidney and skin were removed for the determination of malondialdehyde (MDA) and glutathione (GSH) levels In parallel, tissues were also examined histologically Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, and creatinine and blood urea nitrogen (BUN), concentrations (BUN) were measured to assess liver and kidney functions, respectively Tumor necrosis factor (TNF) and lactate dehydrogenase (LDH) were also assayed in serum samples No statistical differences were found among the control and EndoREZ(R), GuttaFlow(R) and Kerr Pulp Canal sealers regarding tissue MDA, GSH levels or serum parameters (p>0 05) at all time points examined Both of the new root canal sealers showed good compatibility and acceptable tissue toxicity (C) 2009 Elsevier GmbH All rights reservedPublication Metadata only Evaluation of DNA damage in construction-site workers occupationally exposed to welding fumes and solvent-based paints in Turkey(SAGE PUBLICATIONS INC, 2010) BECEREN, AYFER; Sardas, Semra; Omurtag, Gulden Z.; Tozan, Ayfer; Gul, Hulya; Beyoglu, DirenIn this study, the comet assay was used to evaluate whether welding fume and solvent base paint exposure led to DNA damage in construction-site workers in Turkey. The workers (n = 52) were selected according to their exposure in the construction site and controls (n = 26) from the general population, with no history of occupational exposure. The alkaline comet assay, a standard method for assessing genotoxicity, has been applied in peripheral lymphocytes of all subjects. The mean percentages of DNA in tail (%DNA(T)) of each group were evaluated, including the comparisons between smokers in each different group and the duration of exposure. Significant increase in the mean %DNA(T) (p < 0.01) was observed in all exposed subjects (12.34 +/- 2.05) when compared with controls (6.64 +/- 1.43). Also %DNA(T) was significantly high (p < 0.01) in welders (13.59 +/- 1.89) compared with painters (11.10 +/- 1.35). There was a statistical meaningful difference in % DNA(T) between control and exposed smokers. Our findings indicate that exposure to welding fumes and paints induce genotoxic effect in peripheral lymphocytes, indicating a potential health risk for workers. Therefore, to ensure maximum occupational safety, biomonitoring is of great value for assessing the risk for construction workers.Publication Metadata only Protective effect of resveratrol against naphthalene-induced oxidative stress in mice(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2008) BECEREN, AYFER; Sehirli, Oezer; Tozan, Ayfer; Omurtag, Guelden Z.; Cetinel, Sule; Contuk, Gazi; Gedlk, Nursal; Sener, GoekselObjective: This investigation confirms the role of free radicals in naphthalene-induced toxicity and elucidates the mechanism of resveratrol (RVT). Methods: Both male and female BALB-c mice were administered with naphthalene (100mg/kg, i.p.) for 30 days, either along with saline or along with RVT (10 mg/kg, orally). At the end of the experiment, following treatment and sacrifice of animals by decapitation, lung, liver and kidney tissue samples were taken for histological examination or determination of malondialdehyde (MDA), glutathione (GSH), myeloperoxidase (MPO) activity and collagen contents. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), blood urea nitrogen (BUN) and creatinine levels and lactate dehydrogenase (LDH) activity were measured in the serum samples, while TNF-alpha, IL-beta, IL-6 and total antioxidant capacity (AOC) were assayed in plasma samples. Results: Naphthalene administration caused a significant decrease in tissue GSH and plasma AOC, which was accompanied with significant increases in tissue MDA and collagen levels and MPO activity. Moreover, the pro-inflammatory mediators (TNF-alpha, IL-beta, IL-6), LDH activity, AST, ALT, creatinine and BUN levels were significantly increased in the naphthalene group. On the other hand, RVT treatment reversed all these biochemical indices as well as histopathological alterations induced by naphthalene. Conclusions: Oxidative mechanisms play an important role in naphthalene-induced tissue damage, and RVT, by inhibiting neutrophil infiltration, balancing oxidant-antioxidant status, and regulating the generation of inflammatory mediators, ameliorates oxidative organ injury due to naphthalene toxicity. (C) 2007 Elsevier Inc. All rights reserved.