Person: YALÇIN, AHMET SUHA
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YALÇIN
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AHMET SUHA
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Publication Metadata only Synergistic Induction of Apoptosis by Quercetin and Curcumin in Chronic Myeloid Leukemia (K562) Cells: II. Signal Transduction Pathways Involved(ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD, 2021) YALÇIN, AHMET SUHA; Altundag, Ergul Mutlu; Yilmaz, Ayse Mine; Serdar, Belgin Sert; Jannuzzi, Ayse Tarbin; Kocturk, Semra; Yalcin, A. SuhaFlavonoids are phenolic substances with chemo-preventive and chemotherapeutic properties. They are widely found in fruits and vegetables. The polyphenols quercetin and curcumin have antioxidant, anti-inflammatory, anti-carcinogenic, and pro-apoptotic properties. They were successfully used against different human cancers, especially chronic myeloid leukemia cancer cells. We have previously investigated anti-proliferative and apoptotic effects of quercetin and curcumin combination in K562 cells. Our data showed that they had beneficial synergistic effects. Based on these findings, we aimed to clarify signaling pathways involved in synergistic combination treatment with quercetin and curcumin in these cells. Proteins were investigated by Western blotting and by confocal microscopy. Changes in several genes in 10 different pathways related to cell proliferation, apoptosis, cell cycle, inflammation, hypoxia and oxidative stress were observed. Combination of quercetin and curcumin was effective on genes that were particularly related to p53, NF-kappa B and TGF-alpha pathways. Down-regulatory (CDKN1B, AKT1, IFN-gamma) and up-regulatory (BTG2, CDKN1A, FAS) effects on genes and related protein expressions may provide a multi-targeted therapy potential for chronic myeloid leukemia cancer cells without affecting healthy cells.Publication Metadata only The potential use of natural products to negate hepatic, renal and neuronal toxicity induced by cancer therapeutics(PERGAMON-ELSEVIER SCIENCE LTD, 2020) YALÇIN, AHMET SUHA; Prsa, Patrik; Karademir, Betul; Bicim, Gokhan; Mahmoud, Hatem; Dahan, Inbal; Yalcin, A. Suha; Mahajna, Jamal; Milisav, IrinaDifferent types of chemotherapeutics are used for cancer treatment. These drugs act on several signal pathways, lead to programmed cell death, and damage cancer cells. Although many specific mechanisms of action have been suggested for chemotherapeutics, there are still gaps in understanding their effects. They may affect different components of the cell, particularly proteins with specific functions, such as enzymes. Recently, targeted and immuno therapies were introduced for treatment of different cancers. However, many cancer patients still depend on traditional and well-known drugs. Doxorubicin and platinum-based drugs are among the most frequently used chemotherapeutics. They are highly cytotoxic for cancer cells, but they also act on healthy cells. Hence, it is crucial to understand the mechanisms involved in order to decrease their side effects. Natural products, many of which are also available over-the-counter, may be considered to decrease various cancer drug-induced side effects. This review focuses on the use of these compounds to overcome side effects of chemotherapeutics, primarily doxorubicin and cisplatin, in the liver, kidney, and neuronal systems.Publication Open Access Migren Hastalığının Patogenezinde Oksidatif Stres, Damar Fizyopatolojisini_x000D_ Etkileyen Faktörler ve Enflamasyonun Rolü(2021-04-28) YALÇIN, AHMET SUHA; ERAY METİN GÜLER;Ülker ANADOL;Hayriye POLAT;Ahmet KILINÇ;Ayşe Destina YALÇIN;A. Suha YALÇINAmaç: Çalışmamızda migren tanısı konmuş hastalar ile sağlıklı kontrol grubu arasında oksidatif stres, damar fizyopatolojisi ve_x000D_ enflamasyon biyobelirteçleri açısından bir fark olup olmadığının araştırılması amaçlandı._x000D_ Materyal ve Metod: SBÜ Ümraniye Eğitim Araştırma Hastanesi Nöroloji Polikliniğine başvuran, 18 - 49 yaş arasında olup migren_x000D_ tanı kriterlerine uyan ve sistemik herhangi bir hastalığı olmayan 27 hasta ile 27 sağlıklı kişiden kan ve idrar örnekleri alındı._x000D_ İdrarda malondialdehit, eritrositlerde glutatyon, glutatyonla ilgili enzimler, süperoksit dismutaz, katalaz, malondialdehit ve_x000D_ protein karbonilleri, plazmada malondialdehit, bilirubin, ürik asit ve albümin gibi oksidatif stres biyobelirteçlerine, damar_x000D_ fizyopatolojisi biyobelirteçlerinden trombosit ve fibrinojene, enflamasyon biyobelirteçlerinden ise interkökin (IL) 1β, IL6, IL10,_x000D_ tümör nekrozis faktör (TNF) α, c reaktif protein (CRP) ve ferritin düzeyleri ölçüldü._x000D_ Bulgular: Hasta grubunda glutatyon ve glutatyonla ilgili enzimlerin yanında süperoksit dismutaz ve katalaz değerleri kontrol_x000D_ grubuna kıyasla istatistiksel olarak anlamlı düşük (p<0,001) bulundu. Plazma albümin düzeylerinde gruplar arasında istatistiksel_x000D_ fark görülmedi. Ürik asit ve total bilirubin düzeylerinde ise hasta grubundaki düzeyler istatistiksel olarak anlamlı yüksek (p<0,001)_x000D_ bulundu. Benzer şekilde oksidatif hasar belirteçleri olan protein karbonilleri ile plazma, eritrosit ve idrar malondialdehit düzeyleri_x000D_ hasta grubunda istatistiksel olarak anlamlı yüksek (p<0,001) bulundu. Damar fizyopatolojisi belirteçlerinden trombosit sayısı ve_x000D_ fibrinojen düzeylerinin hasta grubunda anlamlı olarak arttığı (p<0,001) gözlendi. Enflamasyon belirteçlerinden IL1β, IL6, IL10 ve_x000D_ TNFα düzeyleri hasta grubunda istatistiksel olarak anlamlı yüksek (p<0,001) bulunurken, CRP ve ferritin düzeyleri düşüktü._x000D_ Sonuç: Migren hastalarında oksidatif stres, damar fizyopatolojisi ve enflamasyon belirteçleri birlikte değerlendirildiğinde,_x000D_ hastalardaki baskılanan ve azalan antioksidan düzeylerinin oksidatif stresi arttırdığı dolayısıyla enflamasyon ve damar_x000D_ fizyopatolojisi değişikliklerine neden olduğu sonucuna varıldı.Publication Open Access Effect of whey protein derivatives on cell viability, cell migration and cell cycle phases in MCF-7 cells(2023-01-01) YILMAZ GÖLER, AYŞE MİNE; YALÇIN, AHMET SUHA; Aksoy F. T., YILMAZ GÖLER A. M., Bicim G., Yalcin A. S.Objective: This study aimed to obtain protein derivatives after treatment of whey proteins with hazelnut oil and olive oil and determined their effects on MCF-7 cells. Materials and Methods: Whey proteins obtained from 6% whey powder were treated with hazelnut oil (HO) and olive oil (OO) at a protein to lipid ratio of 1:10 at 60 ̊C for 120 minutes. The protein derivatives formed with whey protein and HO or OO were applied to MCF-7 cancer cells and healthy fibroblasts. The effects of protein derivatives on cell viability, apoptosis, reactive oxygen species (ROS) production, wound healing, cell cycle phase distribution and cell cycle related proteins Akt and p21(Waf1/Cip1) expressions were investigated. Results: Cell viability decreased significantly after 24 h of incubation with WP:OO. The percentage of apoptotic or necrotic cells varied between 5-10% and no statistically significant effect was observed. There was no statistically significant difference in ROS production and colony formation between controls and WP:HO or WP:OO groups. Treatment of cells with WP:OO for 24 h significantly decreased cell migration compared to the control group. G2/M phase was significantly suppressed in WP:OO group compared to the control group. WP:OO also increased the expression of p21(Waf1/Cip1) significantly when compared with the control group. Conclusion: Our results showed that whey protein derivatives applied to MCF-7 cells are cytotoxic and may be useful in breast cancer treatmentPublication Metadata only Synergistic Combination of Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid and Natural Flavonoid Curcumin Exhibits Anticancer and Antibacterial Activity(BENTHAM SCIENCE PUBL LTD, 2021) YALÇIN, AHMET SUHA; Altundag, Ergul M.; Toprak, Kubra; Sanliturk, Gizem; Guran, Mumtaz; Ozbilenler, Cahit; Kerkuklu, Namik R.; Yilmaz, Ayse M.; Yalcin, Ahmet S.Background and Objective: Curcumin is an effective anti-cancer agent used in thyroid cancer treatments. However, its use in clinical applications is limited due to low solubility and bioavailability. In this study, a novel combination strategy was applied by combining curcumin with Suberoylanilide Hydroxamic Acid (SAHA) to increase both bioavailability of curcumin and the efficiency of SAHA, which have limited efficiency when used alone. Methods: MTT assay was used to determine the cell viability of B-CPAP cells upon treatment with SAHA, curcumin and their combinations. Synergistic interactions between two agents were analyzed by Calcusyn software. Apoptosis and cell cycle assays were measured by flow cytometry. Expressions of apoptotic and cell cycle-related proteins (PARP, P21/CDKN1A/WAF1, P27/KIP1) were examined by western blot analysis. Broth microdilution assay was performed to determine Minimum Inhibitory Concentration (MIC) values against S. aureus. Results: Based on MTT assay, IC50 values for SAHA and curcumin were determined as 0.91 mu M and 20.97 mu M, respectively. The combination index CI value was determined as 0.891 in B-CPAP cells, which demonstrate synergistic activity. The apoptotic effect was achieved by combination treatment (51.85%) on B-CPAP cells using half of the dose required for SAHA and curcumin alone. Combination treatment showed a significant increase in the percentage of B-CPAP cells in the S-phase due to cell arrest. Cleaved-PARP, P21/CDKN1A/ WAF1 and P27/KIP1 protein expressions were upregulated. Curcumin was found to have better anti-microbial activity than SAHA as having a lower MIC value, and checkerboard synergy analysis revealed that the two compounds co-operate synergistically for the in-vitro killing of S. aureus. Conclusion: In the present study, synergistic combinations of SAHA and curcumin were shown to have both anti-cancer and antibacterial activities that would provide a novel thyroid cancer treatment strategy.