Person: YILMAZ GÖLER, AYŞE MİNE
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YILMAZ GÖLER
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AYŞE MİNE
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Publication Metadata only Combination of proteasome inhibitors with temozolomide to increase the anti-tumor effect in 3D culture model of glioblastoma(ELSEVIER SCIENCE INC, 2018) YILMAZ GÖLER, AYŞE MİNE; Unal, Semra; Gokce, Tilbe; Arslan, Sema; Yilmaz, Ayse Mine; Gunduz, Oguzhan; Karademir, BetulPublication Metadata only Proteasomal degradation of TP53INP1 and their relation to methotrexate treatment in prostate cancer cells(ELSEVIER SCIENCE INC, 2016) YILMAZ GÖLER, AYŞE MİNE; Cinel, Ali Emin; Kaplan, Gulce Sari; Yilmaz, Ayse Mine; Karademir, BetulPublication Metadata only Chemotherapy Resistance: The role of proteasomal degradation and heat shock response(ELSEVIER SCIENCE INC, 2015) ŞAHİN, ALİ; Karademir, Betul; Sozen, Erdi; Bozaykut, Perinur; Altundag, Ergul Mutlu; Yilmaz, Ayse Mine; Sahin, Ali; Corek, Ceyda; Sari, Gulce; Ozer, Nesrin KartalPublication Metadata only Combination of second-generation proteasome inhibitor carfilzomib with bortezomib in four different breast cancer cell lin(2022-01-01) YILMAZ GÖLER, AYŞE MİNE; ŞAHİN, ALİ; YILMAZ, BETÜL; Altundag E. M., Yilmaz A. M., Sahin A., Yilmaz B.Background: Proteasome inhibitors target different pathways in cells and therefore are promising drugs in cancer therapy. The use of these inhibitors is approved mainly in hematological cancers, and recently many clinical trials and preclinical studies have been conducted on efficacy in solid tumors. Carfilzomib is a second-generation inhibitor and was developed to decrease the side effects of bortezomib. Although there are many valid therapies for breast cancer, resistance and recurrence are inevitable in many cases and the proteasomal system plays an important role in related pathways. Objective: This study is a preliminary work to evaluate the combined effects of bortezomib and carfilzomib in four different breast cancer cells. Methods: MDA-MB-231, MCF-7, UACC-2087, and SKBR-3 cell lines were used. Cell viability was determined using bortezomib and carfilzomib alone and in combination. Combination effect values were determined using the Chou-Talalay method. Apoptosis, proteasome activity, cleaved PARP, and HSP70 expressions were analyzed in the determined doses. Results: The response to the combination of the two inhibitors was different in four cell lines. Apoptosis was significantly higher in combination groups compared to carfilzomib in three cell lines except for SKBR-3, and higher in the combination group compared to bortezomib only in UACC-2087. Combination decreased cleaved PARP levels in MDA-MB-231 and MCF-7 and increased SKBR-3 compared to bortezomib. HSP70 levels decreased in combination with UACC-2087 and SKBR-3 compared to carfilzomib. Conclusion: Taken together, the combination of the two inhibitors was more apoptotic compared to carfilzomib and apoptosis was higher only in UACC-2087 compared to bortezomib. This apoptosis data can not be directly correlated to the degree of proteasome inhibition, PARP cleavage, and HSP70 response.Publication Metadata only Enzimatik aktivite analizleri: kaspazlar ve proteazom(Manisa Celal bayar Üniversitesi Basımevi, 2022-09-01) YILMAZ GÖLER, AYŞE MİNE; YILMAZ, BETÜL; Yılmaz Göler A. M., Yılmaz B.