Person: YÜKSEL, MERAL
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YÜKSEL
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MERAL
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Publication Open Access Vitamin D receptor and estrogen receptor gene polymorphisms in men with type 2 diabetes: Effects on Bone Metabolism(2022-12-01) ÜSTAY, ÖZLEM; YAVUZ, DİLEK; YÜKSEL, MERAL; YAVUZ D., YÜKSEL M., Sancak S., Yazici D., ÜSTAY Ö., Deyneli O., Akalin S.Purpose There is an increased fracture risk in type 2 diabetes mellitus [DM] patients independent of bone mineral density [BMD], both in men and women. Estrogen receptor [ER]-alpha and vitamin D receptor [VDR] gene polymorphisms may predispose patients to increased osteoporosis and fracture risk. This study aims to analyze the relationship of the ER-alpha gene and VDR gene polymorphisms with indicators of bone turnover and BMD in male type 2 diabetic patients. Methods Type 2 diabetic men diagnosed with diabetes for at least one year and healthy controls were included in this cross-sectional study. BMD was measured by dual X ray absorptiometry. Gene polymorphisms were evaluated with polymerase chain reaction-restriction length polymorphism. Serum iPTH, calcium, beta-CrossLaps (cTx), osteocalcin, and free testosterone levels were also evaluated. Results Participants were 141 type 2 diabetic men [55 +/- 8 years] and 100 healthy controls [53 +/- 7 years]. BMD measurements were not statistically different between the groups. While iPTH [p < 0.05] and serum calcium levels [p = 0.03] were higher in men with type 2 DM; beta-CrossLaps [p = 0.0001], osteocalcin [p = 0.005], and free testosterone [p = 0.04] were lower than controls. The differences in terms of the frequencies of VDR Apa, Taq, Bsm, Fok and ER-alpha polymorphisms were not statistically significant between the groups. No relationship was observed between polymorphisms and BMD in both groups. Conclusions VDR and ER-alpha gene polymorphisms seem to have no effect on BMD and bone turnover in men with DM.Publication Metadata only Sıçanlarda parkinson hastalığına bağlı oluşan bellek disfonksiyonuna farklı egzersiz uygulamalarının etkileri(2022-05-12) KOYUNCUOĞLU, TÜRKAN; ÖZKAN YENAL, NAZİYE; KASIMAY ÇAKIR, ÖZGÜR; GÜLHAN, REZZAN; YÜKSEL, MERAL; Koyuncuoğlu T., Erol G., Çulpan Y., Gülhan R., Yüksel M., Özkan Yenal N., Kasımay Çakır Ö.Giriş: Parkinson hastalığı (PH) Alzheimer hastalığından sonra en yaygın görülennörodejeneratif hastalıktır.1 PH’da motor semptomların yanı sıra bellek disfonksiyonugörülmektedir. Yüksek anksiyete seviyeleri bellek fonksiyonlarını olumsuz etkilemektedir.2Amaç: İstemli tekerlek çevirme egzersizi, direnç egzersizi ve kombine egzersizuygulamalarının anksiyete düzeyleri ile bellek üzerindeki etkilerinin araştırılması ve alttayatan mekanizmaların ortaya konulması amaçlanmıştır.Yöntem: Çalışmada Wistar Albino erkek sıçanlarda (n=50) taklit cerrahi ve Parkinsongrupları oluşturulduktan sonra sedanter ve 3 farklı egzersiz protokolünün uygulandığı istemli(İE), rezistans (RE) ve kombine (KE: İE+RE) grupları oluşturuldu. Egzersizler 6 haftaboyunca (3 gün/hafta) uygulandı. PH modeli oluşturmak için sıçanlarda sağ mediyal önbeyine 6-OHDA (0.5 μl/dk) enjeksiyonu yapıldı. Taklit cerrahili gruplara 6-OHDA’nınçözücüsü verildi. Apomorfin uygulamaları sonrası rotasyon hareketi ile Parkinson modelideğerlendirildi. Obje tanıma testi ve delikli levha testleri sıçanlarda bellek fonksiyonlarını veanksiyete düzeylerini değerlendirmek için yaptırıldı. Beyin dokusunda antioksidan glutatyon(GSH) ve lipid peroksidasyonu belirteci malondialdehit (MDA) ve nötrofil infiltrasyonugöstergeci miyeloperoksidaz (MPO) aktivitesi, oksidan radikallerin belirteci luminol velusigenin ölçüldü. Verilerin analizinde tek yönlü ANOVA ardından Tukey-Kramer testi ilestudent’s t testi kullanıldı. Bulgular: Her 3 egzersiz ile Parkinsona bağlı gerileyen bellek fonksiyonu düzelmiştir(p<0.05-0.01). Sedantere kıyasla PH oluşturulmuş KE grubunda rotasyon hareketi azalırken,İE grubunda arttı (p<0.05-0.01). PH oluşturulmasıyla luminol ve lusigenin düzeyleri artarken,İE ile luminol azalmıştır (p<0.05-0.001). MPO aktivitesinin PH oluşturulmasıyla sedantergrubunda yükseldiği, ancak İE ve RE gruplarında baskılandığı gözlenmiştir (p<0.01-0.001).Her 3 egzersiz ile GSH düzeylerinin arttığı (p<0.05-0.01), KE ile MDA düzeylerinin düştüğübulundu (p<0.05). PH oluşturulmasıyla sedanter grupta azalmış bulunan bakılan delik sayısıve şahlanma sayısı (p<0.01-0.001), RE ve KE gruplarında artmıştır (p<0.05-0.01). Sedanterve İE gruplarında artan donma süresi, KE ile azalmıştır (p<0.05-0.001).Tartışma ve Sonuç: KE lipid peroksidasyonunu baskılamış, İE ve RE nötrofilinfiltrasyonunu azaltmıştır. RE ve KE anksiyeteyi hafifletmiştir. PH’da oluşan bellekdisfonksiyonunda farklı egzersiz uygulamalarının koruyucu etki gösterdikleri ortayakonmuştur.Anahtar Sözcükler: Egzersiz, Parkinson, Anksiyete, Bellek, AntioksidanPublication Metadata only The effect of metformin on ethanol- and indomethacininduced gastric ulcers in rats(2022-09-01) YÜKSEL, MERAL; ERCAN, FERİHA; CABADAK, HÜLYA; AYDIN OMAY, BANU; İpek B. E. , YÜKSEL M., Cumbul A., ERCAN F., CABADAK H., AYDIN OMAY B., Alican İ.BACKGROUND: Previous studies found metformin as an effective agent to suppress oxidative stress, inflammation, and apoptosis in various inflammatory diseases. The present study investigated the effect of metformin against 2 experimental gastric injury models in rats, using macroscopical, histopathological, biochemical, and immunostaining studies. METHODS: After 24 hours of fasting, male Sprague-Dawley rats (280-400 g) (n = 8 per group) received indomethacin (80 mg/kg; indo ulcer group) or absolute ethanol (5 mL/kg; ethanol ulcer group) or vehicle orally by gavage. Metformin (500 mg/kg) was given orally for 3 days prior to indomethacin or ethanol challenge. Ranitidine (50 mg/kg) was given orally for 3 days before indomethacin or ethanol administration as a positive control. On day 3, the animals were euthanized 6 hours after indo or 1 hour after ethanol challenge. Gastric samples were used for macroscopic scoring, histopathological examinations, and biochemical assays. Trunk blood was collected for the assessment of interleukin-1β level. RESULTS: In both ethanol ulcer and indo ulcer groups, metformin decreased the extent of gastric lesions macroscopically and microscopically, improved the high chemiluminescence levels, and the percentage of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive apoptotic cells compared with untreated ulcer groups. Gastric blood flow analysis revealed significant increases in both metformin-treated ulcer groups compared to untreated ulcer groups. CONCLUSION: The findings of the present work demonstrated the gastroprotective effect of metformin against the development of gastric mucosal lesions induced by ethanol and indomethacin in non-diabetic, normoglycemic rats via its antioxidant and anti-apoptotic properties and partly from its ability to restore blood flow.Publication Open Access Cerrahi menopoz oluşturulmuş sıçanların karaciğer ve böbrek dokularında oksidan/antioksidan dengenin korunmasında egzersizin ve östrojenin yararlı etkileri(2022-09-01) YÜKSEL, MERAL; ERCAN, FERİHA; YILDIRIM, ALPER; YEGEN, BERRAK; Tamer S. A. , Levent N., Yüksel M., Ercan F., Yıldırım A., Yegen B.Amaç: Bu çalışmanın amacı cerrahi olarak menopoz oluşturulan sıçanların böbrek ve karaciğerlerinde gözlenen histopatolojik ve fonksiyonel değişiklikleri ve östrojen veya egzersizin ya da östrojen-egzersiz kombinasyonunun oksidan hasar üzerine etkilerini araştırmaktır.Materyal ve Metot: Anestezi altında Sprague Dawley dişi sıçanlara (n=32) bilateral overiektomi uygulandı ve tüm sıçanlar rastgele olarak iki gruba ayrıldı. Sıçanların yarısına normal içme suyu, diğer yarısının içme sularına östrojen (1mg/kg/gün) eklendi. İki hafta sonra gruplar kendi içlerinde sedanter ve egzersiz (5 gün/hafta, 30 daki-ka, 8 hafta) gruplarına ayrıldı. Deney protokolünün sonun-da serum, karaciğer ve böbrek örnekleri biyokimyasal ve histopatolojik incelemeler için alındı. Femurda da histopa-tolojik değerlendirme yapıldı.Bulgular: Cerrahi olarak menopoz oluşturulan sıçan-larda östrojenin böbrek dokusunda nötrofil infiltrasyonunu ve reaktif oksijen türlerinin üretimini baskılayarak koruyu-cu etki gösterdiği, kemik kütlesinde hafif düzeyde artışa neden olduğu, ancak karaciğerin antioksidan glutatyon düzeyinde azalmaya yol açtığı belirlenmiştir. Buna karşın, östrojen uygulaması menopozda yapılan egzersiz nedeniy-le karaciğerde oluşan oksidan stresi engellemiştir. Egzer-sizle veya egzersize östrojen tedavisinin eklenmesiyle böbrek fonksiyonları önemli ölçüde etkilenmezken, kemik yapısında tek başına östrojene kıyasla daha olumlu deği-şiklikler gözlenmiştir.Sonuç: Östrojen replasmanı kemik dokusundaki olum-lu etkilerinin yanı sıra karaciğer ve böbrekte oksidan stresi azaltmakta ve özellikle karaciğerde egzersize bağlı gelişen oksidan stresi baskılayarak koruyucu etki göstermektedir.Publication Open Access Nephroprotective effect of aloe vera extract with regulation of oxidative stress, apoptosis and aquaporin 3 expression levels in streptozotocin induced iabetic rats(2023-01-01) YÜKSEL, MERAL; Seker U., Guzel B. C., Sener-Akcora D., Baygeldi S. B., YÜKSEL M., Unay-Demirel O., Soker S.Objective: In this study we examined the protective activity of Aloe vera with considering antioxidant, anti-apoptotic properties, and the status of Aquaporin 3 (AQP3) channel protein. Material and Method: Twenty-one adult female rats were divided into three groups (n=7); Control, Diabetes, Treatment. Control group did not expose to any application. Animals in Diabetes and Treatment were exposed to experimental diabetes with administration of streptozotocin. Rats in Treatment received 300 mg/kg Aloe vera extract daily for 14 days. Rats were sacrificed and kidney samples were used for analyses. Result and Discussion: Analyses indicated that lowest malondialdehyde (MDA) and luminol levels in control group were increased significantly (P<0.05) in diabetic animals. Severe pathological changes observed in Diabetes group while microscopic examinations. Bax, Caspase-3 and apoptotic index (AI) were elevated significantly (P<0.05) in this group compared to Control. Oxidative stress, apoptotic protein expression levels and TUNEL Assay positive cell ratio were down-regulated in Treatment group. When AQP3 levels were measured, immunopositivity reduced significantly (P<0.05) in cortical kidney of Diabetes group which is normalized significantly in Treatment group.This study reporting anti-diabetic potency of Aloe vera extract has capability to avoid streptozotocin induced diabetic renal injury via regulating anti-apoptotic and anti-oxidant cellular signaling. Furthermore, Aloe vera consumption in diabetes might regulate AQP3 levels. Although we observed promising results, more studies are required to explore anti-diabetic, anti-hyperglycemic and nephroprotective activity of Aloe vera.Publication Metadata only 83: phoenixin-14 ameliorates cholestatic liver injury and bileinduced acute pancreatic injury in rats(2022-05-01) KAHRAMAN, MERVE MERİÇ; YÜKSEL, MERAL; YEGEN, BERRAK; ERCAN, FERİHA; Şen L. S. , Kahraman M. M. , Mermer K. S. , Köroğlu K., Yüksel M., İmeryüz N., Ercan F., Yegen B.Background: Bile duct obstruction, which results in cholestatic liver injury, is also the major cause of acute pancreatitis. Phoenixin (PNX) was originally defined as a hypothalamic peptide associated with a wide range of physiological processes and exerts antioxidant and antiinflammatory effects. PNX is expressed in several peripheral organs including pancreas and liver. We aimed to evaluate possible therapeutic effects of PNX on hepatic and pancreatic damage induced by biliary or pancreaticobiliary duct obstruction. Methods: In male Sprague Dawley rats, bile duct ligation (BDL; n=16) or pancreaticobiliary duct ligation (PBDL; n= 16) was performed under ketamine anesthesia, while control rats (n=8) had sham-surgery. Either PNX-14 (50 µg/kg/day) or saline was subcutaneously injected immediately after surgery and in the following 2 days. On the post-operative 3rd day, hepatic and renal blood flow was measured using laser Doppler flowmeter under anesthesia and the rats were then euthanized. In the liver and pancreas samples, levels of malondialdehyde, antioxidant glutathione and myeloperoxidase activity were measured by spectrophotometry, while luminol- and lucigenin-enhanced chemiluminescence (CL) levels were measured to assess formation of reactive oxygen species (ROS). Tissue samples were stained by hematoxylin-eosin to calculate microscopic damage scores. Statistical analyses were made by one-way ANOVA. Results: Increased microscopic damage scores in the pancreas of saline-treated PBDL (p<0.001) and in the liver of saline-treated BDL group (p<0.001) were reduced by PNX14 treatment (p<0.01), but PBDL-induced hepatic damage (p<0.001) was not changed by PNX-14. Pancreatic and hepatic levels of malondialdehyde and myeloperoxidase activity and pancreatic glutathione levels were not different among the experimental groups, while hepatic glutathione level was elevated in PNX-14-treated BDL (p<0.001) and PBDL (p<0.01) groups as compared to control group. Despite a non-significant fall in renal or hepatic blood flow in saline-treated BDL rats, PBDL significantly reduced hepatic (p<0.001) and renal (p<0.01) blood flow, while PNX-14 reversed blood flow in both organs back to control levels (p<0.05). The CL levels of luminol and lucigenin were increased in both hepatic and pancreatic tissues of saline-treated BDL and PBDL groups (p<0.05-0.001), showing enhanced ROS generation. However, CL levels in both the liver and pancreas of PNX-14-treated BDL and PBDL groups were significantly reduced as compared to those measured in the liver and pancreas of respective saline-treated groups (p<0.05-0.001). Conclusion: In conclusion, cholestatic liver injury and bile-induced pancreatic injury are alleviated by PNX-14 treatment, which appears to act via its ROS scavenging activity, by replenishing hepatic antioxidant capacity and restoring impaired organ perfusion.Publication Metadata only Irisin protects against neuroinflammation in rats induced by epileptic seizure(2022-09-01) YÜKSEL, MERAL; Ozdemir-Kumral Z. N. , Akgun T., Hasim C., Ulusoy E., Yuksel F., Kalpakcioglu M. K. , Okumus T., Uslu Z., Akakin D., Goren Z., et al.Publication Open Access Paraoxonase-1 and fetuin-A levels in children with cerebral palsy(2022-01-01) YÜKSEL, MERAL; Unay Demirel O., Gungor O., Ignak Tarlig S., YÜKSEL M.Background/aim: Studies mostly focused on risk factors and clinical status in children with cerebral palsy (CP). Various antiinflammatory markers may help us in the early diagnosis and clinical classification of cerebral palsy patients. In this study, the relationship between antiinflammatory marker levels and clinical status in patients with CP is determined. It is the first time that Fetuin-A and Paraoxonase-1 (PON-1) are examined in children with CP. Materials and methods: The study is conducted on 79 children which are divided into two groups as CP and control. Gross motor function and spasticity are evaluated in addition to biochemical parameters. Results: There is a statistically significant difference between CP and control group in terms of PON-1 activity, high sensitive C-Reactive Protein, HDL, and total cholesterol levels. There is no statistically significant difference in Fetuin A levels between the two groups. Conclusion: In suspected CP patients less than 24 months of age who possess prenatal and postnatal risk factors, the determination of PON-1 activity can be considered as a biomarker to support early diagnosis.Publication Metadata only Stimulation of estrogen receptors attenuates oxidant skin injury in hyperglycemic rats with incisional wound(2023-01-01) KAHRAMAN, MERVE MERİÇ; YÜKSEL, MERAL; ERCAN, FERİHA; YEGEN, BERRAK; Sen L. S., Akgun T., KAHRAMAN M. M., YÜKSEL M., ERCAN F., YEGEN B.Publication Metadata only Investigation of the possible protective effect of phoenixin-14 on small intestine and lung damage due to mesenteric ischemia in rats(2023-01-01) KAHRAMAN, MERVE MERİÇ; YÜKSEL, MERAL; ERCAN, FERİHA; YEGEN, BERRAK; YEGEN, ŞEVKET CUMHUR; Sen L. S., Kahraman M. M., Mermer K. S., Akgun T., YÜKSEL M., ERCAN F., YEGEN B., Yegen C.