Person: AKYÜZ, SERAP HATİCE
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
AKYÜZ
First Name
SERAP HATİCE
Name
16 results
Search Results
Now showing 1 - 10 of 16
Publication Metadata only Salivary lipid peroxidation and total sialic acid levels during healthy gestation and postpartum: A longitudinal study(PERGAMON-ELSEVIER SCIENCE LTD, 2010) YARAT, AYŞEN; Ozturk, L. Koc; Akyuz, S.; Yarat, A.; Koc, S.; Gul, N.; Dogan, B. N.Objectives: This study investigated salivary lipid peroxidation (LPO) as an oxidative stress marker and salivary total sialic acid (TSA) as an inflammatory response during gestation and postpartum. Design and methods: Salivary LPO and TSA levels, using the Ledwozyw and Warren methods respectively, were obtained in healthy pregnant women followed up during gestation and 6-8 weeks postpartum, and in healthy non-pregnant controls. All were with good oral health. Results: LPO was significantly higher than controls during all trimesters and postpartum and in the second trimester than in the third trimester and postpartum. TSA in the second trimester was significantly higher than in any other group. First trimester levels were significantly higher than postpartum. Oral health indices remained within normal levels for the duration. Conclusion: The salivary LPO profile followed plasma gestation and postpartum profiles in the literature but the salivary TSA differed in that after the 2nd trimester, rather than persisting, it decreased. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.Publication Metadata only Isolation and Characterization of Dental Pulp Stem Cells from a Patient with Papillon-Lefevre Syndrome(ELSEVIER SCIENCE INC, 2013) YARAT, AYŞEN; Tasli, Pakize Neslihan; Tapsin, Sidika; Demirel, Sezin; Yalvac, Mehmet Emir; Akyuz, Serap; Yarat, Aysen; Sahin, FikrettinIntroduction: Papillon-Lefevre syndrome (PLS) is a rare autosomal recessive disorder. characterized by immune dysregulation because of a mutation in cathepsin c gene, resulting in hyperkeratosis of the palms, soles, elbows, and knees combined with premature loss of the primary and permanent dentitions. Periodontal tissue abnormalities in PLS patients were reported previously. However, less is known about dental pulp tissue derived cells of PLS patients. This study aimed to show stem cell potential of PLS dental pulp stem cells (DPSCs) and provide new evidence regarding the pathophysiology of the disease. Methods: DPSCs were characterized by using flow cytometry and immunocytochemistry. They were also induced to differentiate into adipogenic, osteogenic, chondrogenic, odontogenic, and myogenic cells. Results: The results revealed that PLS DPSCs are stained positive for mesenchymal stem cells surface markers CD29, CD73, CD90, CD105, and CD166. PLS DPSCs were able to differentiate into adipogenic, osteogenic, chondrogenic, and odontogenic cell types properly. PLS DPSCs expressed embryonic stem cell markers Oct4, Sox2, cMYc, and Klf4 and showed similar proliferation rate compared with DPSCs isolated from healthy young controls. Interestingly, it was found that unlike the healthy DPSCs, PLS DPSCs are not able to form myotubes with correct morphology. Conclusions: These data are being reported for the first time; therefore, they might provide new insights to the pathology of the disease. Our results suggest that the PLS DPSCs might be an autologous stem cell source for PIS patients for cellular therapy of alveolar bone defects and other dental tissue-abnormalities observed in PLS. (J Endod 2013;39:31-38)Publication Metadata only The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults(SPRINGER, 2012) YARAT, AYŞEN; Ozturk, Leyla Koc; Ulucan, Korkut; Akyuz, Serap; Furuncuoglu, Halit; Bayer, Hikmet; Yarat, AysenThe aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lower in diabetic patients than those of healthy subjects (P < 0.05). Salivary flow rate, CA activity and DMFT levels did not differ between groups (P > 0.05). Four SNPs were detected; their pubmed database number are rs2274327 (C/T), rs2274328 (A/C), rs2274329 (G/C) and rs2274330. While first three of those were responsible for amino acid changes, the last one was not. The frequencies of SNPs were not significant between groups (P > 0.05). Positive significant correlation was found between CA activity and the frequency of SNPs. There was no correlation between the SNPs frequencies and pH or buffering capacity. SNPs found in this study may be related to salivary CA activity in diabetics.Publication Metadata only Factors Influencing Career Choices of Dental Students in a State University in Turkey(MARMARA UNIV, INST HEALTH SCIENCES, 2018) YARAT, AYŞEN; Capan, Belen Sirinoglu; Akyuz, Serap; Bahcecik, Nefise; Yarat, Aysen; Girgin, FatihObjective: The aim of this study was to investigate the factors influencing the career choices of students in Marmara University Faculty of Dentistry in the 2014-2015 academic year. Methods: Data were collected from 468 dental students by using a 55-item questionnaire. Results: Mean age of students were 21.6 (range 17-25); 300(64.1%) of them were female and 168(35.9%) were male. Two hundred two (43.2%) students indicated that their first choice was dentistry. If they were to make a choice for a second time, 196(41.9%) of students would again prefer dentistry as their career. There was a significant difference between the year of study and the statement I will prefer dentistry again (p<0.05). In fact, 45(49.5%) of first-year students claimed that they would choose dentistry again, whereas 35(41.7%) of fifth-year students stated the opposite. As being passed to higher classes, the percentage of students who will prefer dentistry again decreased. One hundred forty one (30.1%) of all students reported wanting to change their career. It was stated as reasons for changing careers that being a dental student was stressful. Students were most influenced by their family (61.4%). Four hundred eight (87.2%) students were determined to choose dentistry because of its high income. 399 (85.2%) students stated that dentistry is a prestigious profession and this criterion was significant in their career choices. Conclusion: The majority of students who preferred Marmara University, Faculty of Dentistry were girls, and there were many factors influence the career choices and future plans of the students, with the most important beings were the families, the high income level and the prestige of the profession.Publication Metadata only Prevalence of Candida species in Turkish children: relationship between dietary intake and carriage(PERGAMON-ELSEVIER SCIENCE LTD, 2005) AKYÜZ, SERAP HATİCE; Kadir, T; Uygun, B; Akyuz, SIn this study, the prevalence and intensity of Candida species were evaluated in 300 healthy Turkish children aged between 0 and 12 years. The candidal carriage in 26 children who were fed only with breast milk and 38 children who were fed with both breast milk and bottle milk or other fluids was also examined. Oral samples cultured for fungal growth and Candida species were identified using germ tube test, chlamydospore formation test and API 20C AUX system. The results demonstrated that the prevalence of oral candidal carriage in 300 healthy children was 26.3%. Candida albicans was the most frequently isolated yeast (84.8% of the isolates). The other yeasts were identified as Candida parapsilosis, Candida krusei, Candida kefyr, Candida famata, and Candida tropicalis. It was also observed that the frequency of carriage varied as a function of age. The prevalence of carriage in children who were fed with both breast milk and bottle milk or other fluids was 18.5%, while in children fed only with breast milk was 0%. This finding supports previously reported observations that there may be intrinsic differences in oral carriage of Candida species between different ages and populations and type of dietary intake may affect frequency of carriage. (C) 2004 Elsevier Ltd. All rights reserved.Publication Metadata only Salivary sialic acid, protein, salivary flow rate, pH, buffering capacity and caries indices in subjects with Down's syndrome(1999) YARAT, AYŞEN; Yarat, A.; Akyüz, S.; Koç, L.; Erdem, H.; Emekli, N.OBJECTIVES: The aim of this study was to compare salivary sialic acid, protein, salivary flow rate, pH and buffering capacity and caries indices between subjects with Down's Syndrome and healthy controls. METHODS: Unstimulated mixed saliva was collected from 26 Down's syndrome subjects and 25 healthy subjects of age range 6-24 years. Total protein was determined by the method of Lowry and total sialic acid using Ehrlich reagent. Laemmli SDS-polyacrylamide gel electrophoresis was also carried out. RESULTS: Buffering capacity and pH were quite similar for both groups. For permanent dentition subjects pH was significantly higher (P = 0.03) in the Down's syndrome group. The salivary flow rate of the Down's syndrome subjects was significantly lower (P < 0.01) than that of healthy controls and the Down's syndrome subjects' salivary protein and sialic acid levels were significantly higher (P < 0.001). The ratios of total sialic acid to total protein were significantly higher (P < 0.001) in the Down's syndrome group. However, salivary sialic acid expectoration rates, a means of compensating for flow rate differences, were significantly lower (P = 0.01) in the Down's syndrome subjects than in controls. Electrophoresis revealed no significant differences between the protein bands of the groups. There were no significant differences in caries indices between groups, even when compensated for age, nor in the salivary parameters within groups between sexes. CONCLUSIONS: Total salivary sialic acid in Down's syndrome subjects, higher in terms of levels but lower in terms of expectoration rates, was significantly different from that of controls of similar caries indices.Publication Metadata only Masticatory parameters of children with and without clinically diagnosed caries in permanent dentition(ARIESDUE SRL, 2017) AKYÜZ, SERAP HATİCE; Kaya, M. S.; Akyuz, S.; Guclu, B.; Diracoglu, D.; Yarat, A.Aim Mastication turns food into a bolus and prepares it for chemical digestion. Any condition affecting tooth structure and position may have an impact on mastication. The aim of this study is to compare masticatory performance (MP) and maximum bite force (MBF) between children with and without clinically visible caries in permanent first molars. Materials and methods The study was conducted with 50 children in good general condition aged 12-14 years (25 girls, 25 boys) with no orthodontic/skeletal anomalies and no missing teeth due to dental trauma or extraction. Maximum bite force was measured bilaterally using strain gauge sensors. Masticatory performance was evaluated by silicone tablet comminution test. Results Masticatory performance was superior in caries-free children when compared to children with caries. Maximum bite force values in children with and without caries were not statistically different. Conclusions Caries reduces masticatory performance. Therefore, treatment is crucial for masticatory efficiency.Publication Metadata only Two-colour chewing gum mixing ability test for evaluating masticatory performance in children with mixed dentition: validity and reliability study(WILEY, 2017) AKYÜZ, SERAP HATİCE; Kaya, M. S.; Guclu, B.; Schimmel, M.; Akyuz, S.The unappealing taste of the chewing material and the time-consuming repetitive task in masticatory performance tests using artificial foodstuff may discourage children from performing natural chewing movements. Therefore, the aim was to determine the validity and reliability of a two-colour chewing gum mixing ability test for masticatory performance (MP) assessment in mixed dentition children. Masticatory performance was tested in two groups: systemically healthy fully dentate young adults and children in mixed dentition. Median particle size was assessed using a comminution test, and a two-colour chewing gum mixing ability test was applied for MP analysis. Validity was tested with Pearson correlation, and reliability was tested with intra-class correlation coefficient, Pearson correlation and Bland-Altman plots. Both comminution and two-colour chewing gum mixing ability tests revealed statistically significant MP differences between children (n = 25) and adults (n = 27, both P < 0.01). Pearson correlation between comminution and two-colour chewing gum mixing ability tests was positive and significant (r = 0.418, P = 0.002). Correlations for interobserver reliability and test-retest values were significant (r = 0.990, P = 0.0001 and r = 0.995, P = 0.0001). Although both methods could discriminate MP differences, the comminution test detected these differences generally in a wider range compared to two-colour chewing gum mixing ability test. However, considering the high reliability of the results, the two-colour chewing gum mixing ability test can be used to assess masticatory performance in children, especially at non-clinical settings.Publication Metadata only Mycological and cytological examination of oral candidal carriage in diabetic patients and non-diabetic control subjects: thorough analysis of local aetiologic and systemic factors(BLACKWELL PUBLISHING LTD, 2002) AKYÜZ, SERAP HATİCE; Kadir, T; Pisiriciler, R; Akyuz, S; Yarat, A; Emekli, N; Ipbuker, AIn this study, 55 diabetic patients and 45 non-diabetic control subjects were examined to determine oral candidal carriage state. The influence of some local aetiologic and systemic factors such as: salivary flow rate and pH, heredity, alcohol drinking, smoking habits, antimicrobial therapy, wearing of denture, burning sensation, dry mouth, taste alteration and tooth brushing habit on candidal carriage rate were investigated. Imprint culture, cytological smears and biochemical tests were used. Oral carrier rate and density of Candida species were non-significantly higher in the diabetic patients than in the non-diabetic control subjects. This increase was confirmed cytologically too. In both groups, Candida albicans was found to be a predominant species on tongue dorsum. Cigarette and alcohol habits of men were higher while tooth brushing habit was less than in women in diabetic and control groups. Salivary flow rate and pH values of diabetic patients were significantly lower while serum glucose values were significantly higher than of non-diabetic controls. The rate of diabetic patients suffering from dry mouth and having diabetic heredity in the family were significantly higher than control subjects. The candidal colonization was higher and keratinization was lower while diabetic treatment tended from diet and oral antidiabetic towards insulin. The decrease in salivary pH, the increase in serum glucose and wearing denture were correlated with the increased rate and density of C. albicans in both groups. Keratinization was also accompanied with the increase in leucocytes. In diabetic group, positive correlations were found between antimicrobial therapy and C. glabrata carriage; the increase in leucocytes and C. albicans carriage; the increase in keratinization and alcohol habit; serum glucose and smoking habit; dry mouth complaint and antimicrobial therapy. There was a negative correlation between salivary flow rate and C. albicans carriage. In control group a positive correlation was found between antimicrobial therapy and keratinization.Publication Metadata only Fluoride levels in various black tea, herbal and fruit infusions consumed in Turkey(PERGAMON-ELSEVIER SCIENCE LTD, 2009) YARAT, AYŞEN; Emekli-Alturfan, Ebru; Yarat, Aysen; Akyuz, SerapThe fluoride contents were determined by ion-selective electrode in 26 black tea samples originally produced in Turkey, Sri Lanka, India and Kenya, and in 14 herbal and seven fruit infusions originated from Turkey. Fluoride content in black tea infusions ranged from 0.57 to 3.72 mg/L after 5 min of brewing. Higher fluoride levels were found in black teas originated from Turkey when compared with teas originated from Sri Lanka. Moreover higher fluoride levels were determined in black tea bags compared with granular and stick-shaped black teas. However, herbal and fruit infusions were characterized by low values of fluoride (0.02-0.04 mg/L) after 5 min of brewing and increasing brewing time to 10 min caused only slight increases in some infusions. As a result, consuming tea infusions prepared from some black tea available in Turkish market, especially black tea bags, in large quantities may lead to exposion to a high amount of fluoride which may cause dental fluorosis. Although fruit and herbal infusions are safer to consume their fluoride contents are too low for caries prevention. In countries such as Turkey where tea is traditionally consumed, the fluoride concentration and daily safety precautions should be indicated on tea products. (C) 2009 Elsevier Ltd. All rights reserved.