Person: AKYÜZ, SERAP HATİCE
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AKYÜZ
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SERAP HATİCE
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Publication Metadata only Salivary lipid peroxidation and total sialic acid levels during healthy gestation and postpartum: A longitudinal study(PERGAMON-ELSEVIER SCIENCE LTD, 2010) YARAT, AYŞEN; Ozturk, L. Koc; Akyuz, S.; Yarat, A.; Koc, S.; Gul, N.; Dogan, B. N.Objectives: This study investigated salivary lipid peroxidation (LPO) as an oxidative stress marker and salivary total sialic acid (TSA) as an inflammatory response during gestation and postpartum. Design and methods: Salivary LPO and TSA levels, using the Ledwozyw and Warren methods respectively, were obtained in healthy pregnant women followed up during gestation and 6-8 weeks postpartum, and in healthy non-pregnant controls. All were with good oral health. Results: LPO was significantly higher than controls during all trimesters and postpartum and in the second trimester than in the third trimester and postpartum. TSA in the second trimester was significantly higher than in any other group. First trimester levels were significantly higher than postpartum. Oral health indices remained within normal levels for the duration. Conclusion: The salivary LPO profile followed plasma gestation and postpartum profiles in the literature but the salivary TSA differed in that after the 2nd trimester, rather than persisting, it decreased. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.Publication Metadata only The investigation of genetic polymorphisms in the carbonic anhydrase VI gene exon 2 and salivary parameters in type 2 diabetic patients and healthy adults(SPRINGER, 2012) YARAT, AYŞEN; Ozturk, Leyla Koc; Ulucan, Korkut; Akyuz, Serap; Furuncuoglu, Halit; Bayer, Hikmet; Yarat, AysenThe aim of this study was to investigate carbonic anhydrase (CA) VI Exon 2 single nucleotide polymorphism (SNP) and its possible association with salivary parameters in type 2 diabetic patients compared to healthy adults. Caries status was measured by using the DMFT (number of decayed, missing, and filled teeth) index. Unstimulated whole saliva and blood samples were taken. SNPs of CA gene exon 2 were determined by PCR and DNA sequencing. Salivary CA activity and buffering capacity were determined by the method of Verpoorte and Ericson, respectively. Furthermore, salivary pH was measured with pH paper and salivary flow rate was calculated. Salivary buffering capacity and pH were significantly lower in diabetic patients than those of healthy subjects (P < 0.05). Salivary flow rate, CA activity and DMFT levels did not differ between groups (P > 0.05). Four SNPs were detected; their pubmed database number are rs2274327 (C/T), rs2274328 (A/C), rs2274329 (G/C) and rs2274330. While first three of those were responsible for amino acid changes, the last one was not. The frequencies of SNPs were not significant between groups (P > 0.05). Positive significant correlation was found between CA activity and the frequency of SNPs. There was no correlation between the SNPs frequencies and pH or buffering capacity. SNPs found in this study may be related to salivary CA activity in diabetics.Publication Open Access The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints(BMC, 2017-12) YARAT, AYŞEN; Bicak, Damla Aksit; Akyuz, Serap; Kiratli, Binnur; Usta, Merve; Urganci, Nafiye; Alev, Burcin; Yarat, Aysen; Sahin, FikrettinBackground: The oral cavity can be an extra-gastric reservoir for Helicobacter pylori (H. pylori). This can play a role in the pathogenesis of halitosis, glossitis, recurrent aphthous stomatitis, and dental caries. The present study was conducted to detect the presence of H. pylori within the dental biofilm and in saliva samples collected from children suffering from dyspepsia and children without any gastrointestinal complaints. Associations with gastric infection, halitosis, and some oral parameters were also evaluated. Methods: Seventy children (aged between 5-16) with dyspepsia were selected for the study group and control group composed of 30 healthy children without dyspepsia were also included in the study. After detailed oral and clinical examinations for oral parameters, saliva, and supragingival dental biofilm samples were collected for 16S rRNA and 23S rRNA genes detection by real-time polymerase chain reaction (RT-PCR). The presence of gastric H. pylori was evaluated in endoscopic biopsy specimens histopathologically. Halitosis was evaluated by benzoylDL-arginine-naphthylamid (BANA) test. Salivary S. mutans and Lactobacilli sp. counts were also carried out by commercial kits. Results: H. pylori was histopathologically detected amongst 83% of the children with the dyspeptic condition. The detection rate of this bacteria in dental biofilm and saliva samples and halitosis were found relatively higher in the dyspeptic children rather than the control group (p < 0.01). Halitosis was not significantly different between dyspeptic children and those detected with H. pylori (p > 0.05). In the gastric H. pylori positive group with dyspepsia, DMFT/ S and dmft/ s numbers and plaque indices were found higher than the control group (p < 0.01). Only plaque indices of gastric H. pylori negative group with dyspepsia were found higher than the control group (p < 0.01). S. mutans and Lactobacilli sp. counts were not significantly different between gastric H. pylori positive and negative groups (p > 0.05). Comparing to those with negative for both genes, in children whose dental biofilm and saliva samples were positive for both 16S rRNA and 23S rRNA genes, significantly higher results for halitosis, and DMFS numbers and significantly lower results for dmfs numbers and pH values were found (p < 0.01). Conclusions: Helicobacter pylori can occur in the oral cavity aside and independently from the stomach. However, the high number of bacteria in the oral cavities of children with gastric H. pylori, an association between the presence of H. pylori and halitosis, DMFS, and pH were found.Publication Metadata only Salivary sialic acid, protein, salivary flow rate, pH, buffering capacity and caries indices in subjects with Down's syndrome(1999) YARAT, AYŞEN; Yarat, A.; Akyüz, S.; Koç, L.; Erdem, H.; Emekli, N.OBJECTIVES: The aim of this study was to compare salivary sialic acid, protein, salivary flow rate, pH and buffering capacity and caries indices between subjects with Down's Syndrome and healthy controls. METHODS: Unstimulated mixed saliva was collected from 26 Down's syndrome subjects and 25 healthy subjects of age range 6-24 years. Total protein was determined by the method of Lowry and total sialic acid using Ehrlich reagent. Laemmli SDS-polyacrylamide gel electrophoresis was also carried out. RESULTS: Buffering capacity and pH were quite similar for both groups. For permanent dentition subjects pH was significantly higher (P = 0.03) in the Down's syndrome group. The salivary flow rate of the Down's syndrome subjects was significantly lower (P < 0.01) than that of healthy controls and the Down's syndrome subjects' salivary protein and sialic acid levels were significantly higher (P < 0.001). The ratios of total sialic acid to total protein were significantly higher (P < 0.001) in the Down's syndrome group. However, salivary sialic acid expectoration rates, a means of compensating for flow rate differences, were significantly lower (P = 0.01) in the Down's syndrome subjects than in controls. Electrophoresis revealed no significant differences between the protein bands of the groups. There were no significant differences in caries indices between groups, even when compensated for age, nor in the salivary parameters within groups between sexes. CONCLUSIONS: Total salivary sialic acid in Down's syndrome subjects, higher in terms of levels but lower in terms of expectoration rates, was significantly different from that of controls of similar caries indices.Publication Open Access Assessment of dental caries and salivary nitric oxide levels in children with dyspepsia(BMC, 2019-12) ALTURFAN, EBRU IŞIK; Aksit-Bicak, Damla; Emekli-Alturfan, Ebru; Ustundag, Unsal Veli; Akyuz, SerapBackground: The increase in nitric oxide (NO) levels in the oral cavity and saliva have been associated with various oral diseases; however, the gastro-salivary interaction of NO remains controversial. Thus, the aim of this study was to determine and compare salivary NO levels of dyspeptic and non-dyspeptic healthy children and to conduct an evaluation of its association with dental caries. Methods: Seventy children with dyspepsia (dyspeptic group) and 30 children without any gastrointestinal complaints (control group) were included in the study. Two biopsies from the gastric tissues were collected from dyspeptic children for histopathologic examination. Oral examination involved the assessment of dental caries, gingival index, plaque index, buffering capacity, salivary flow rate and pH. Salivary Streptococcus mutans (S. mutans) and Lactobacilli sp. counts were performed by commercial kits. For the comparison of the normal distribution between dyspeptic and control groups, Student t-test and for the comparison of the non-normal distribution, Kruskal-Wallis and Mann-Whitney-U tests were used. Chi-square test was used for comparison of qualitative data and the Pearson correlation test was used to evaluate the association between certain variables. Significance was assessed at p<0.05 level. Results: Helicobacter pylori (H.pylori) were found in gastric biopsies of 84.2% (59/70) of the dyspeptic children. While the mean salivary NO values did not differ significantly between gastric H.pylori positive, negative and control groups, the salivary NO level of the dyspeptic group (213.7 +/- 51.68 mu mol/dL) was found to be significantly higher than the control group (185.7 +/- 16.66 mu mol/dL). No significant relationship was found between the mean salivary NO values, DMFT/dmft numbers and other oral parameters. Conclusions: The association of dental caries and salivary NO levels could not be considered specific in the current study. Although there were no statistically significant differences between salivary NO levels of gastric H.pylori positive, gastric H.pylori negative and control groups, greater salivary NO levels among dyspeptic children compared with the control group demonstrated that the concentration of NO in the saliva could be used as a biological marker in dyspepsia, which could lead to the improvement of more specified, uncomplicated and susceptible methods for analysis.Publication Metadata only Boron-rich diet may regulate blood lipid profile and prevent obesity: A non-drug and self-controlled clinical trial(ELSEVIER GMBH, 2019) YARAT, AYŞEN; Kuru, Ruya; Yilmaz, Sahin; Balan, Gulsah; Tuzuner, Burcin Alev; Tasli, Pakize Neslihan; Akyuz, Serap; Ozturk, Feyza Yener; Altuntas, Yuksel; Yarat, Aysen; Sahin, FikrettinBackground: Boron is an element commonly found in nature. The main boron source for organisms is through food and drinking water. In recent years, it is suggested that the boron-rich diet can affect human health positively. However, more detailed studies are needed. Objective: The aim of this study was to examine the effect of increased dietary boron intake on some biochemical parameters in humans. Material and methods: Thirteen healthy women consumed diets containing 10 mg more boron than their routine diet for one month. This boron intake was provided with the increase of boron-rich foods such as dried fruits, avocado, and nuts in the diet. Some biochemical and hematologic parameters were determined in blood, urine and saliva samples taken before and after a boron-rich diet. Results: Serum, salivary, and urine boron concentrations increased 1.3, 1.7, 6.0 fold, respectively. The most significant clinically change was found in the lipid profile. Serum total, LDL, VLDL cholesterol, and triglyceride levels decreased significantly. Body weight, body fat weight, and Body Mass Index also decreased. Significant changes in serum TSH and salivary buffering capacity were also found. Conclusion: Increasing the intake of boron through dietary means might contribute to beneficial effects on lipid metabolism, obesity, and thyroid metabolism; salivary boron may reflect serum boron; and boron may be used as a cariostatic agent in dentistry. An increased intake of other dietary factors such as fiber, potassium, iron, vitamin A, and vitamin E in the boron-rich foods might have been responsible of the effects described. To our knowledge, this study is the first clinical study in which dietary boron intake is increased via foods.