Person: ŞEHİRLİ, ÜMİT SÜLEYMAN
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ŞEHİRLİ
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ÜMİT SÜLEYMAN
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Publication Open Access Cerebellar connections to the rostral reticular nucleus of the thalamus in the rat(WILEY, 2002-12) ONAT, FİLİZ; Cavdar, S; Onat, FYL; Yananli, HR; Sehirli, US; Tulay, C; Saka, E; Gurdal, EWe studied the cerebellar connections to the reticular nucleus thalamus (RNT) by means of retrograde axonal transport of horseradish peroxidase (HRP) in the rat. Specific HRP pressure injections to the rostral RNT(1.6-1.8 mm caudal to bregma) resulted in retrograde labelling of neurones in the cerebellar nuclei. The rostral RNT showed specific topographical organization of its cerebellar connections. Microinjections into the rostral RNT, 1.6 mm caudal to bregma, produced numerous HRP-labelled neurones within the anterior interposed (emboliform nucleus) and scarce HRP-labelled neurones within the lateral (dentate nucleus) cerebellar nuclei, whereas injections into the rostral RNT, 1.8 mm caudal to bregma, produced numerous HRP-labelled neurones within the posterior interposed (globose nucleus) and scarce lightly HRP-labelled neurones within the lateral (dentate nucleus) cerebellar nuclei. Cerebellar connections with the rostral RNT were exclusively ipsilateral to the injection site. No HRP-labelled cells were detected in the medial (fastigial nucleus) cerebellar nucleus. The cerebellar connections reach the RNT via the superior cerebellar peduncle. By contrast, HRP injections into the anterior, posterior interposed and lateral cerebellar nuclei produced no labelled cells within the RNT. This study demonstrates the existence of direct cerebello-RNT but not RNT-cerebellar connections. The presence of the cerebello-RNT connections introduces a new route through which the cerebellum may influence RNT and thus cerebral cortical activity.Publication Open Access The effects of melatonin on the striatum(MARMARA UNIV, FAC MEDICINE, 2021-10-31) BORACI, HATİCE; Gergin, Sinem; Kirazli, Ozlem; Boraci, Hatice; Yildiz, Sercan Dogukan; Sehirli, Umit SuleymanObjective: Some of the neurological diseases cause morphologic changes in the striatal neurons. Medial forebrain bundle (MFB) lesion is a commonly used method to produce a Parkinsonian model rat. Melatonin is a hormone which exerts a neuroprotective effect on the neurons. The aim of this study is to investigate the effect of melatonin on the dendritic morphology of striatal medium spiny neurons (MSNs) in rats with MFB lesion. Materials and Methods: Twelve male Wistar albino rats were given saline injections into the MFB and divided into sedentary and treatment groups. The treatment group was administered a 10 mg/kg dose of melatonin intraperitoneally for 30 days. The lesion was confirmed histologically by Nissl staining. Golgi staining technique was applied to observe neuronal morphology. Neuronal structures were analysed from three-dimensional images by Neurolucida (MBF Bioscience) software. Results: The MFB lesion caused a reduction in the total dendritic length and in the number of dendritic endings. The melatonin enhanced the number of dendritic endings compared to the sedentary group. The melatonin led to an increase in the total spine density, spine densities of thin and mushroom types. Conclusion: Melatonin improved the dendritic degeneration due to MFB lesion.Publication Open Access The failure on the effectiveness of formalin on cadaver disinfection and alternative methods(2023-01-01) AKSU, MEHMET BURAK; ÖZKAN, MAZHAR; ŞEHİRLİ, ÜMİT SÜLEYMAN; Yanilmaz O., Guncu M. M., AKSU M. B., ÖZKAN M., ŞEHİRLİ Ü. S.Objective: It was aimed to identify the contaminant and determine the alternative disinfectant detection in the microbial growth observed in various parts of the cadaver stored in the formalin tank in the dissection laboratory of Marmara University Anatomy Department. We also performed a literature review of this unusual pathogen. Materials and Methods: Swab samples were inoculated on agar mediums. After incubation, matrix-assisted laser desorption ionizationtime of flight mass spectrometry (MALDI-TOF MS) analysis was used to identify the isolate from the detected uniform colonies. Sample solution from the cadaver tank, freshly prepared 5% formalin and 0.55% ortho-phthalaldehyde were used to determine the disinfectant sensitivity of the isolate. Results: According to 16s rDNA sequence analysis, it was concluded as Skermanella aerolata with 99% similarity. In the disinfectant susceptibility test, it was observed that S. aerolata and control bacteria could grow in 5% formalin taken from the cadaver tank. No growth was detected in other disinfectants. Conclusion: To prevent cadaver contamination in anatomy laboratories, the quality control of the embalming solutions and indoor air filtration of the dissection rooms should be checked at regular intervals. Members of Skermanella genus have been identified as environmental organisms in several studies, however, recent researches reported this bacterium as a human pathogen.Publication Open Access The effects of regular swimming exercise and melatonin on the neurons localized in the striatum of hemiparkinsonian rats(2022-10-01) KİRAZLI, ÖZLEM; BORACI, HATİCE; YILDIZ, SERCAN DOĞUKAN; YANANLI, HASAN RACİ; ŞEHİRLİ, ÜMİT SÜLEYMAN; Gergin S., KİRAZLI Ö., BORACI H., YILDIZ S. D. , YANANLI H. R. , ŞEHİRLİ Ü. S.Parkinson's disease is a progressive neurodegenerative movement disorder. We aimed to investigate the effects of regular swimming exercise and melatonin applied in the 6-Hydroxydopamine-induced Parkinson's disease rats by analysing dendritic spine of striatal neurons. Twenty-four male Wistar albino rats were used. 6-Hydroxydopamine unilaterally injected four (control, exercise, melatonin and exercise + melatonin) groups were included in the study. Tyrosine hydroxylase expression was detected by immunohistochemistry. Neurons and structures were identified from three-dimensional images by Neurolucida software. There was not any apparent difference for tyrosine hydroxylase positive neurons in the substantia nigra pars compacta and fibres in the striatum between the lesion sides of hemiparkinsonian groups. The treatment groups blocked the apomorphine-induced increase in rotations compared to the control group. In stepping test, the treatment groups prevented the loss of stepping in the contralateral side of hemiparkinsonian groups. The melatonin mostly had a positive effect on motor activity tests. In morphological analyses, the 6-Hydroxydopamine-induced lesion led to the reduction of the total dendritic length and number of branches. In the treatment groups, the reduction of the dendritic parameters was not observed. 6-Hydroxydopamine lesion led to a decrease in the total spine density, spine densities of thin and mushroom types. The exercise and melatonin treatments prevented the loss of spine density. The exercise treatment prevented the loss of spine density of mushroom type spines. The melatonin treatment blocked the loss of spine density of stubby type. In conclusion, these results provide evidence for effective additional protective therapeutic strategies for Parkinson\"s disease. In conclusion, results from the current study provide evidence for swimming exercise and melatonin as a promising candidate for effective additional protective strategies for PD.Publication Open Access The Effect of Canagliflozin on Hippocampal Dendrite Morphology in a Model of Alzheimer's Disease Induced by Intracerebroventricular Injection of Streptozotocin(2023-10-01) YAZI, SEVDENUR; YANANLI, HASAN RACİ; ŞEHİRLİ, ÜMİT SÜLEYMAN; KİRAZLI, ÖZLEM; Yazı S., Özen B., Buldu B., Yalçın E., Karaköse O., Çakmak O., Somunkıran S., Yananlı H. R., Şehirli Ü. S., Kirazlı Ö.Publication Open Access Developing a wearable device for upper extremity tremors(2024-05-31) AKGÜN, GAZİ; GÜNAL, DİLEK; AKÜNER, MUSTAFA CANER; ŞEHİRLİ, ÜMİT SÜLEYMAN; Yildiz S. D., AKGÜN G., GÜNAL D., Kaplanoglu E., AKÜNER M. C., ŞEHİRLİ Ü. S.Objective: This project aims to develop a wearable device to suppress both the essential and resting tremor and investigate its effectiveness. Materials and Methods: This study details the development and assessment of a wearable device for upper extremity tremors. The wearable device underwent a comprehensive design and a prototype was produced with a 3D-printer. To refine the functionality of the prototype, a motor that mimics tremor was attached to a 3D-printed prototype. Then, the printed prototype was applied to the hand model, and tested its effectiveness for tremor suppressing. The wearable device was further investigated on patients with essential tremor and Parkinson`s disease seeking treatment at Neurology Clinics. We recorded the tremor data and processed and visualized the recorded data by using the MatLab (version R2021a, MathWorks Inc., USA) software. Results: The wearable device effectively decreased the tremors both during the simulation phase and the patient testing phase. The data from the wearable device revealed a notable decrease in the amplitude of the tremor. This decrease signifies an achievement of tremor suppression. Conclusion: The prototype of the wearable device signifies a remarkable efficacy in tremor supression. It holds promise for being a potential solution to alleviate the tremor symptoms of essential tremor and Parkinson`s disease patients.Publication Open Access Dopamine acting at D1-like, D2-like and alpha 1-adrenergic receptors differentially modulates theta and gamma oscillatory activity in primary motor cortex(PUBLIC LIBRARY SCIENCE, 2017-07-21) ŞEHİRLİ, ÜMİT SÜLEYMAN; Ozkan, Mazhar; Johnson, Nicholas W.; Sehirli, Umit S.; Woodhall, Gavin L.; Stanford, Ian M.The loss of dopamine (DA) in Parkinson's is accompanied by the emergence of exaggerated theta and beta frequency neuronal oscillatory activity in the primary motor cortex (M1) and basal ganglia. DA replacement therapy or deep brain stimulation reduces the power of these oscillations and this is coincident with an improvement in motor performance implying a causal relationship. Here we provide in vitro evidence for the differential modulation of theta and gamma activity in M1 by DA acting at receptors exhibiting conventional and nonconventional DA pharmacology. Recording local field potentials in deep layer V of rat M1, co- application of carbachol (CCh, 5 mu M) and kainic acid (KA, 150 nM) elicited simultaneous oscillations at a frequency of 6.49 +/- 0.18 Hz (theta, n = 84) and 34.97 +/- 0.39 Hz (gamma, n = 84). Bath application of DA resulted in a decrease in gamma power with no change in theta power. However, application of either the D1-like receptor agonist SKF38393 or the D2-like agonist quinpirole increased the power of both theta and gamma suggesting that the DA-mediated inhibition of oscillatory power is by action at other sites other than classical DA receptors. Application of amphetamine, which promotes endogenous amine neurotransmitter release, or the adrenergic alpha 1-selective agonist phenylephrine mimicked the action of DA and reduced gamma power, a result unaffected by prior co- application of D1 and D2 receptor antagonists SCH23390 and sulpiride. Finally, application of the alpha 1-adrenergic receptor antagonist prazosin blocked the action of DA on gamma power suggestive of interaction between alpha 1 and DA receptors. These results show that DA mediates complex actions acting at dopamine D1-like and D2-like receptors, a1 adrenergic receptors and possibly DA/alpha 1 heteromultimeric receptors to differentially modulate theta and gamma activity in M1.Publication Open Access A study on brain asymmetry in temporal lobe epilepsy(2024-05-31) ŞEHİRLİ, ÜMİT SÜLEYMAN; Bilisli Kara E., Firat Z., Ulug A. M., Ekinci G., ŞEHİRLİ Ü. S.Objective: Temporal lobe epilepsy (TLE) accompanied by hippocampal sclerosis (HS) is the most common type of focal epilepsies. Hemispheric asymmetry is a feature of brain organization in both invertebrates and vertebrates and may be the key to some neurodegenerative diseases. In this context, we aimed to investigate the volumetric asymmetry difference in cerebral structures between TLE patients and the healthy control group, based on magnetic resonance imaging (MRI) data that may be used as a new neuroimaging marker for TLE cases. Patients and Methods: In this retrospective study the cranial MRIs of fourteen clinically manifesting, radiologically HS-identified, and diagnosed TLE patients and fourteen healthy individuals from the Radiology Department of Yeditepe University Hospital were evaluated. Volume measurements and asymmetry index (AI) calculations in the total brain, hippocampus, temporal lobe, amygdala, thalamus, nucleus accumbens (NAc), premotor cortex, primary and somatosensory cortices were performed using the medical NeuroQuant® software. A negative AI value represented asymmetry towards the right due to reduced left hemispheric volume; a positive AI value represented asymmetry towards the left due to reduced right hemispheric volume. Subsequently, differences in volume and asymmetric patterns were investigated among TLE subgroups (right and left-sided TLE) and controls. Results: The left-sided TLE patients showed significant bilateral total brain volume reduction compared to the control group. Significant ipsilateral volumetric declines were also detected in the premotor cortex, the temporal lobe, and NAc with remarkable asymmetry to the right side. No significant changes were detected in right-sided TLE patients compared to the other groups. Conclusion: Overall, findings suggest that TLE patients had volumetric alterations with symmetry changes beyond the mesial temporal structures. With further investigations, the asymmetry measures can provide additional knowledge for TLE diagnosis. Keywords: Brain asymmetry, Temporal lobe epilepsy, Asymmetry index, Nucleus accumbens