Person: ÇAM, MUHAMMET EMİN
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ÇAM
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MUHAMMET EMİN
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Publication Metadata only Development of Satureja cuneifolia-loaded sodium alginate/polyethylene glycol scaffolds produced by 3D-printing technology as a diabetic wound dressing material(ELSEVIER, 2020) OKTAR, FAİK NÜZHET; Ilhan, Elif; Cesur, Sumeyye; Guler, Ece; Topal, Fadime; Albayrak, Deniz; Guncu, Mehmet Mucahit; Cam, Muhammet Emin; Taskin, Turgut; Sasmazel, Hilal Turkoglu; Aksu, Burak; Oktar, Faik Nuzhet; Gunduz, OguzhanAcute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe. This study aims to produce and characterize Satureja cuneifolia plant extract (SC) blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer. SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA. The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively. Mechanical and thermal properties, swelling, and degradation behaviours were also investigated. The release kinetics of SC were performed. The antimicrobial analysis was evaluated against Escherichia coli and Staphylococcus aureus strains. 3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain. Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay. The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections. (c) 2020 Elsevier B.V. All rights reserved.Publication Metadata only Vitamin D-3/vitamin K-2/magnesium-loaded polylactic acid/tricalcium phosphate/polycaprolactone composite nanofibers demonstrated osteoinductive effect by increasing Runx2 via Wnt/beta-catenin pathway(ELSEVIER, 2021) ÇAM, MUHAMMET EMİN; Guler, Ece; Baripoglu, Yaren Ezgi; Alenezi, Hussain; Arikan, Ayca; Babazade, Ravana; Unal, Semra; Duruksu, Gokhan; Alfares, Fawzan S.; Yazir, Yusufhan; Oktar, Faik Nuzhet; Gunduz, Oguzhan; Edirisinghe, Mohan; Cam, Muhammet EminVitamin D3, vitamin K2, and Mg (10%, 1.25%, and 5%, w/w, respectively)-loaded PLA (12%, w/v) (TCP (5%, w/ v))/PCL (12%, w/v) 1:1 (v/v) composite nanofibers (DKMF) were produced by electrospinning method (ES) and their osteoinductive effects were investigated in cell culture test. Neither pure nanofibers nor DKMF caused a significant cytotoxic effect in fibroblasts. The induction of the stem cell differentiation into osteogenic cells was observed in the cell culture with both DKMF and pure nanofibers, separately. Vitamin D3, vitamin K2, and magnesium demonstrated to support the osteogenic differentiation of mesenchymal stem cells by expressing Runx2, BMP2, and osteopontin and suppressing PPAR-gamma and Sox9. Therefore, the Wnt/beta-catenin signaling pathway was activated by DKMF. DKMF promoted large axonal sprouting and needle-like elongation of osteoblast cells and enhanced cellular functions such as migration, infiltration, proliferation, and differentiation after seven days of incubation using confocal laser scanning microscopy. The results showed that DKMF demonstrated sustained drug release for 144 h, tougher and stronger structure, higher tensile strength, increased water up-take capacity, decreased degradation ratio, and slightly lower Tm and Tg values compared to pure nanofibers. Consequently, DKMF is a promising treatment approach in bone tissue engineering due to its osteoinductive effects.Publication Metadata only Controlled release of donopezil-loaded polyvinyl alcohol (pva) microbubbles/nanoparticles using microfluidic t-junction device(2020-09-18) CESUR, SÜMEYYE; ÇAM, MUHAMMET EMİN; GÜNDÜZ, OĞUZHAN; Cesur S., Çam M. E., Gündüz O.Production of donepezil- loaded microbubble/nanoparticles for treatment of Alzheimer diseasePublication Metadata only Fabrication, characterization and fibroblast proliferative activity of electrospun Achillea lycaonica-loaded nanofibrous mats(PERGAMON-ELSEVIER SCIENCE LTD, 2019) KABASAKAL, LEVENT; Cam, Muhammet Emin; Cesur, Sumeyye; Taskin, Turgut; Erdemir, Gokce; Kuruca, Durdane Serap; Sahin, Yesim Muge; Kabasakal, Levent; Gunduz, OguzhanThe use of natural compounds such as biocompatible and non-toxic plant extracts, without undesired side effects, in tissue engineering applications, is highly preferred compared to chemical drugs. In this study, the characterization and performance of electrospun Achillea lycaonica-loaded (0.125, 0.250 and 0.500, wt%) poly (lactic acid) (PLA) (8%, w/v) nanofibrous mats for skin tissue engineering were investigated. SEM, FTIR, DSC, and tensile strength test of the electrospun nanofibers have been investigated. Drug releasing test and cell culture study were also carried out. Achillea lycaonica-loaded nanofibrous mats in 0.250 (wt%) and 0.500 (wt%) demonstrated excellent cell compatibility and increased the viability of NIH/3T3 (mouse embryo fibroblast) cells within 72 h. According to the results, Achillea lycaonica-loaded PLA nanofibers have proper tensile strength and controlled release. The working temperature range enlarged for the composites having higher plant extract content. Consequently, Achillea lycaonica-loaded nanofibrous mats have a great potential in skin tissue engineering applications.Publication Metadata only Controlled Release of Metformin Loaded Polyvinyl Alcohol (PVA) Microbubble/Nanoparticles Using Microfluidic Device for the Treatment of Type 2 Diabetes Mellitus(Springer, 2020) SAYIN, FATİH SERDAR; Cesur S., Cam M.E., Sayın F.S., Su S., Gunduz O.Nowadays it became obvious that a relentless increase in Type 2 diabetes mellitus (T2DM), affecting the economically affluent countries, is gradually afflicting also the developing world. The currently used drugs in the treatment of T2DM have inefficient glucose control and carry serious side effects. In this study, nano-sized uniform particles were produced by microfluidic method by the explosion of microbubbles. Morphological (SEM), molecular interactions between the components (FT-IR), drug release test by UV spectroscopy measurement were carried out after production process. When microbubbles and nanoparticles, optical microscope and SEM images obtained were examined, it was observed that metformin was successfully loaded into nanoparticles. The diameter of the microbubbles and nanoparticles was 104 ± 91 µm and 116 ± 13 nm, respectively. Metformin was released in a controlled manner at pH 1.2 for 390 min. It is promising in the treatment of T2DM with the controlled release ability of metformin loaded nonoparticles. © Springer Nature Switzerland AG 2020.Publication Metadata only Electrically controlled drug release of donepezil and BiFeO3 magnetic nanoparticle-loaded PVA microbubbles/nanoparticles for the treatment of Alzheimer's disease(Editions de Sante, 2021) SAYIN, FATİH SERDAR; Cesur S., Cam M.E., Sayin F.S., Gunduz O.Nanocarriers are used to deliver bioactive substances in the treatment of neurodegenerative diseases such as Alzheimer's disease (AD). These nanocarriers have shown many benefits over traditional treatments due to their properties such as efficient distribution and controlled release of bioactive material to the brain and loading of various drugs simultaneously. In this study, polyvinyl alcohol (PVA), PVA/bismuth ferrite (BiFeO3), and PVA/BiFeO3/donepezil hydrochloride (DO) monodisperse polymeric nanoparticles were manufactured with bursting microbubbles by a T-junction device. Here, BiFeO3 nanoparticles were synthesized by the co-precipitation method, and these magnetic nanoparticles and DO were loaded in PVA nanoparticles. Nanoparticles had a smooth and monodisperse structure according to SEM images. Also, the diameters of PVA, PVA/BiFeO3, and PVA/BiFeO3/DO nanoparticles were 148 ± 15 nm, 159 ± 21 nm, and 164 ± 12 nm, respectively. It was confirmed by X-ray diffraction and infrared spectroscopy that BiFeO3 magnetic nanoparticles and DO were successfully loaded into nanoparticles produced with PVA. There was no cytotoxic effect on healthy L929 cells for all nanoparticle samples. A systematic electrical circuit has been established to investigate the electrically controlled release behaviour of PVA/BiFeO3/DO nanoparticles at different voltages (0 V, −1.0 V, −0.5 V, +0.5 V, +1.0 V), different currents (−50 μA, −100 μA, −200 μA, and −300 μA), and 200 rpm. To apply electric stimulus increased the release except for +1.0 V and the release of DO increased at more negative voltages with a total release of 68.9% of DO after 15 stimulus with −1.0 V. Higher R2 values were obtained with the Higuchi model for almost all conditions and DO was released from nanoparticles through the non-Fickian diffusion mechanism (0.45 < n < 1). The possibility of affecting the release of DO by modifying the current and voltage in the presence of BiFeO3 leads to an immensely controllable and delicately tunable drug release for AD treatment. © 2021Publication Metadata only Metformin-Loaded Polymer-Based Microbubbles/Nanoparticles Generated for the Treatment of Type 2 Diabetes Mellitus(American Chemical Society, 2021) ÇAM, MUHAMMET EMİN; Cesur S., Cam M.E., Sayln F.S., Su S., Harker A., Edirisinghe M., Gunduz O.Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease that is increasingly common all over the world with a high risk of progressive hyperglycemia and high microvascular and macrovascular complications. The currently used drugs in the treatment of T2DM have insufficient glucose control and can carry detrimental side effects. Several drug delivery systems have been investigated to decrease the side effects and frequency of dosage, and also to increase the effect of oral antidiabetic drugs. In recent years, the use of microbubbles in biomedical applications has greatly increased, and research into microactive carrier bubbles continues to generate more and more clinical interest. In this study, various monodisperse polymer nanoparticles at different concentrations were produced by bursting microbubbles generated using a T-junction microfluidic device. Morphological analysis by scanning electron microscopy, molecular interactions between the components by FTIR, drug release by UV spectroscopy, and physical analysis such as surface tension and viscosity measurement were carried out for the particles generated and solutions used. The microbubbles and nanoparticles had a smooth outer surface. When the microbubbles/nanoparticles were compared, it was observed that they were optimized with 0.3 wt % poly(vinyl alcohol) (PVA) solution, 40 kPa pressure, and a 110 μL/min flow rate, thus the diameters of the bubbles and particles were 100 ± 10 μm and 70 ± 5 nm, respectively. Metformin was successfully loaded into the nanoparticles in these optimized concentrations and characteristics, and no drug crystals and clusters were seen on the surface. Metformin was released in a controlled manner at pH 1.2 for 60 min and at pH 7.4 for 240 min. The process and structures generated offer great potential for the treatment of T2DM. ©Publication Metadata only Fabrication and characterization of achillea Iycaonica- loaded poly(Lactic Acid) nanofibers(2018-09-14) ÇAM, MUHAMMET EMİN; CESUR, SÜMEYYE; TAŞKIN, TURGUT; GÜNDÜZ, OĞUZHAN; Çam M. E., Cesur S., Taşkın T., Kabasakal L., Gündüz O.Publication Metadata only Dual-drug delivery of Ag-chitosan nanoparticles and phenytoin via core-shell PVA/PCL electrospun nanofibers(ELSEVIER SCI LTD, 2021) ŞAHİN, ALİ; Hussein, Mohamed Ahmed Mohamady; Guler, Ece; Rayaman, Erkan; Cam, Muhammet Emin; Sahin, Ali; Grinholc, Mariusz; Mansuroglu, Demet Sezgin; Sahin, Yesim Muge; Gunduz, Oguzhan; Muhammed, Mamoun; El-Sherbiny, Ibrahim M.; Megahed, MosaadDual-drug delivery systems were constructed through coaxial techniques, which were convenient for the model drugs used the present work. This study aimed to fabricate core-shell electrospun nanofibrous membranes displaying simultaneous cell proliferation and antibacterial activity. For that purpose, phenytoin (Ph), a well-known proliferative agent, was loaded into a polycaprolactone (PCL) shell membrane, and as-prepared silver-chitosan nanoparticles (Ag-CS NPs), as biocidal agents, were embedded in a polyvinyl alcohol (PVA) core layer. The morphology, chemical composition, mechanical and thermal properties of the nanofibrous membranes were characterized by FESEM/STEM, FTIR and DSC. The coaxial PVA-Ag CS NPs/PCL-Ph nanofibers (NFs) showed more controlled Ph release than PVA/PCL-Ph NFs. There was notable improvement in the morphology, thermal, mechanical, antibacterial properties and cytobiocompatibility of the fibers upon incorporation of Ph and Ag-CS NPs. The proposed core-shell PVA/PCL NFs represent promising scaffolds for tissue regeneration and wound healing by the effective dual delivery of phenytoin and Ag-CS NPs.