Person: ÇAM, MUHAMMET EMİN
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ÇAM
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MUHAMMET EMİN
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Publication Open Access A novel treatment strategy for preterm birth: Intra-vaginal progesterone-loaded fibrous patches(ELSEVIER, 2020-10) YAVUZ, AYŞE NUR; Cam, Muhammet Emin; Hazar-Yavuz, Ayse Nur; Cesur, Sumeyye; Ozkan, Ozan; Alenezi, Hussain; Sasmazel, Hilal Turkoglu; Eroglu, Mehmet Sayip; Brako, Francis; Ahmed, Jubair; Kabasakal, Levent; Ren, Guogang; Gunduz, Oguzhan; Edirisinghe, MohanProgesterone-loaded poly(lactic) acid fibrous polymeric patches were produced using electrospinning and pressurized gyration for infra-vaginal application to prevent preterm birth. The patches were intravaginally inserted into rats in the final week of their pregnancy, equivalent to the third trimester of human pregnancy. Maintenance tocolysis with progesterone-loaded patches was elucidated by recording the contractile response of uterine smooth muscle to noradrenaline in pregnant rats. Both progesterone-loaded patches indicated similar results from release and thermal studies, however, patches obtained by electrospinning had smaller average diameters and more uniform dispersion compared to pressurized gyration. Patches obtained by pressurized gyration had better results in production yield and tensile strength than electrospinning; thereby pressurized gyration is better suited for scaled-up production. The patches did not affect cell attachment, viability, and proliferation on Vero cells negatively. Consequently, progesterone-loaded patches are a novel and successful treatment strategy for preventing preterm birth.Publication Open Access Experimental and theoretical investigation of the fluid behavior during polymeric fiber formation with and without pressure(AMER INST PHYSICS, 2019-12) ÇAM, MUHAMMET EMİN; Alenezi, Hussain; Cam, Muhammet Emin; Edirisinghe, MohanThe fabrication of polymeric micro/nanofibers is gaining attention due to their use in an array of applications including tissue engineering scaffolds, nanosensors, and fiber-reinforced composites. Despite their versatile nature, polymeric fibers are widely underutilized due to the lack of reliable, large-scale production techniques. Upon the discovery of centrifugal spinning and, recently, pressurized gyration techniques, new research directions have emerged. Here, we report a comprehensive study detailing the optimal conditions to significantly improve the morphology, homogeneity, and yield of fibers of varying diameters. A series of polymeric fibers was created using a 21 wt.% solution of polyethylene oxide in distilled water and the fluid behavior was monitored inside a transparent reservoir using a high-speed camera. Fabrication of the fibers took less than 1 s. Using centrifugal spinning, we studied the formation of the fibers at three different rotational speeds, and for pressurized gyration, one rotational speed was studied with three different nitrogen gas pressures. Using the pressurized gyration technique at a gas pressure of 0.3 MPa, there was significant improvement in the production yield of the fibers. We found a strong correlation between the variation of pressure and the rate of the solution leaving the reservoir with the improved morphology of the fibers. The use of reduced power techniques, like centrifugal spinning and pressured gyration, to yield high-quality nonwoven nanofibers and microfibers in large quantities is important due to their use in rapidly expanding markets. (C) 2019 Author(s).Publication Open Access Camellia Sinensis Leaves Hydroalcoholic Extract Improves the Alzheimer's Disease-Like Alterations Induced by Type 2 Diabetes in Rats(MARMARA UNIV, INST HEALTH SCIENCES, 2020-06-29) ÇAM, MUHAMMET EMİN; Cam, Muhammet Emin; Taskin, TurgutObjective: Novel investigations have confirmed that hyperglycemia is strictly associated with the development of cognitive impairment and dementia. Sodium-dependent glucose transporter (SGLT) inhibitors, which are oral antidiabetic drugs, are currently being investigated as the medication in Alzheimer's disease (AD). In our study, Camellia sinensis (green tea), which inhibits sodium-dependent glucose transporter-1 (SGLT-1), was used in the treatment of type 2 diabetes mellitus (T2DM)-induced AD-like alterations via its antidiabetic effects. Methods: High-fat diet/streptozotocin-treated rat model was chosen to provide T2DM-induced AD-like alterations. Antidiabetic effects were evaluated with the measurement of blood glucose level (BGL), oral glucose tolerance test (OGTT), and insulin tolerance test (ITT). On the other hand, novel object recognition test (NORT), open field test (OFT), passive avoidance test (PAT), and Morris's water maze (MWM) test were performed to investigate the anti-Alzheimer's effects of C. Sinensis. Results: C. sinensis tolerated BGL for a short time but metformin, the first medication prescribed for T2DM, tolerated BGL during the test for 120 min. C. sinensis increased the number of square crosses and the frequency of grooming activity in a similar manner to metformin in OFT. C. sinensis treatment improved exploratory behavior and memory retention components in NORT. The step-through latency decreased in HFD/STZ-treated rat model but it improved with metformin and C. sinensis treatment in PAT. According to the results obtained by the MWM test, C. sinensis treatment slightly improved learning. Conclusion: C. sinensis improved short-term memory and increased the locomotor activity in rats according to the results obtained by NORT, OFT, and PA.Publication Open Access Levodopa-Loaded 3D-Printed Poly (Lactic) Acid/Chitosan Neural Tissue Scaffold as a Promising Drug Delivery System for the Treatment of Parkinson's Disease(MDPI, 2021-11-13) ŞAHİN, ALİ; Saylam, Ezgi; Akkaya, Yigit; Ilhan, Elif; Cesur, Sumeyye; Guler, Ece; Sahin, Ali; Cam, Muhammmet Emin; Ekren, Nazmi; Oktar, Faik Nuzhet; Gunduz, Oguzhan; Ficai, Denisa; Ficai, AntonParkinson's disease, the second most common neurodegenerative disease in the world, develops due to decreased dopamine levels in the basal ganglia. Levodopa, a dopamine precursor used in the treatment of Parkinson's disease, can be used as a drug delivery system. This study presents an approach to the use of 3D-printed levodopa-loaded neural tissue scaffolds produced with polylactic acid (PLA) and chitosan (CS) for the treatment of Parkinson's disease. Surface morphology and pore sizes were examined by scanning electron microscopy (SEM). Average pore sizes of 100-200 mu m were found to be ideal for tissue engineering scaffolds, allowing cell penetration but not drastically altering the mechanical properties. It was observed that the swelling and weight loss behaviors of the scaffolds increased after the addition of CS to the PLA. Levodopa was released from the 3D-printed scaffolds in a controlled manner for 14 days, according to a Fickian diffusion mechanism. Mesenchymal stem cells (hAD-MSCs) derived from human adipose tissue were used in MTT analysis, fluorescence microscopy and SEM studies and confirmed adequate biocompatibility. Overall, the obtained results show that PLA/CS 3D-printed scaffolds have an alternative use for the levodopa delivery system for Parkinson's disease in neural tissue engineering applications.Publication Open Access Electrosprayed microparticles: a novel drug delivery method(TAYLOR & FRANCIS LTD) ÇAM, MUHAMMET EMİN; Cam, Muhammet Emin; Zhang, Yue; Edirisinghe, MohanPublication Open Access Effects of Cornus mas L. on lipid peroxidation and anti-oxidative enzyme activity in high fat diet fed rats(2023-02-01) KARĞIN, DİCLE; YAVUZ, AYŞE NUR; ÇAM, MUHAMMET EMİN; AKTAÇ, ŞULE; Karğın D., Aktaç Ş., Yavuz A. N., Çam M. E.Cornelian cherry (Cornus mas L.) has been used for centuries as a traditional herbal medicine in Europe and Asia. In this study, we aimed to describe the effect of Cornus mas L. (C. mas) on the activity of the antioxidant enzymes and a detoxification agent in rats fed a high-fat diet. Forty-eight adult Sprague Dawley rats were randomly assigned to six groups of eight animals each: Standard diet (Control), High Fat Diet (HFD), HFD + C. mas (200 mg/kg/day; 8 weeks), HFD + Atorvastatin (20 mg/kg/day; 8 weeks), HFD post-treated with C. mas (200 mg/kg/day; 4 weeks), HFD posttreated with Atorvastatin (20 mg/kg/day; 4 weeks). The activity of the antioxidant enzymes, Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), detoxification agent glutathione (GSH), and oxidative stress parameter thiobarbituric acid reactive substances (TBARS) were measured in the liver tissues. GPx, SOD, and CAT enzyme levels were decreased in HFD groups compared to the control (p < 0.05). However, Cornus mas L. promoted antioxidant activity by increasing GPx, SOD, and CAT enzymes and It also reduced oxidative stress (as an increase in GSH) both in the HFD + C. mas group and the HFD post-treated C. mas group compared to the HFD group (p < 0.05). Our study showed that feeding a high-fat diet increases oxidative stress. Cornus mas L treatment improves antioxidant enzyme activity and oxidative stress parameters in the liver tissues of rats.Publication Open Access A Comparison Study of Fiber Diameter's Effect on Characteristic Features of Donepezil/Curcumin-Loaded Polycaprolactone/Polylactic Acid Nanofibers(2022-05-01) EKENTOK ATICI, CEYDA; GÜNDÜZ, OĞUZHAN; ÇAM, MUHAMMET EMİN; TATAR, ESRA; YAVUZ, AYŞE NUR; Aydin S., Kabaoglu I., Guler E., Topal F., YAVUZ A. N., EKENTOK ATICI C., TATAR E., Gurbuz F., GÜNDÜZ O., ÇAM M. E.Nanofibers (NFs) offer an alternative option for the treatment of Alzheimer\"s disease (AD) by addressing unmet clinical problems. In this study, anti-AD drugs, donepezil (DO) and curcumin (CUR), are loaded in polylactic acid/polycaprolactone NFs. The effect of fiber diameter on drug release behavior is mainly observed, and the successful loading of DO and CUR to NFs is demonstrated. The tensile strength of DO/CUR-loaded NFs (DNFs) with lower fiber diameter is found to be higher. The working temperature is increased by the decrease of glass transition temperature and increase of the melting temperature after loading drugs. Furthermore, the increase in the percentage of swelling and decrease in the degradation rate for NFs are observed due to the increase of fiber diameter. Encapsulation efficiency and burst release percentages for DNFs are augmented by the increase of fiber diameter. Nevertheless, DNFs exhibit a sustained drug release manner over 2 weeks. NFs do not demonstrate a toxic effect on L929 (mouse fibroblast) cells, and additionally, they promote cell proliferation. Considering all these results, it is proven that the fiber diameter affects all characteristic features of NFs, and DNFs lead to a new and promising drug delivery system for the treatment of AD.Publication Open Access Antioxidant and anti-inflammatory activities of Phlomis pungens and Coridothymus capitatus(MARMARA UNIV, FAC PHARMACY, 2018-01-12) YAVUZ, AYŞE NUR; Taskin, Turgut; Cam, Muhammet Emin; Bulut, Gizem; Hazar-Yavuz, Ayse Nur; Kabasakal, Levent; Bitis, LeylaThe purpose of this study was to comparatively reveal for the first time the antioxidant, anti-inflammatory effects of Phlomis pungens and Coridothymus capitatus methanol extracts obtained by using maceration extraction method. According to the results of anti-inflammatory activity, P. pungens methanolic extract demonstrated a more prominent and intensive antiinflammatory effect with 24.7% of inhibitive capacity in the altered edema size after the first hour of carrageenan injection compared to C. capitatus methanolic extract. P. pungens methanolic extract inhibitory effect increased during three hours and reached maximum by 41.9%. According to the results obtained from antioxidant activity experiments, methanol extract of C. capitatus exhibited stronger free radical scavenging (DPPH), cupric reducing (CUPRAC), ferric reducing/antioxidant power (FRAP) and ABTS radical cation scavenging activity than P. pungens extract. In additon, C. capitatus extract had higher ABTS radical cation scavenging and ferric reducing/antioxidant power activity than ascorbic acid, BHT and BHA, respectively. Therefore, this extract can be used in both medicine and food industry as a natural antioxidant source.Publication Open Access The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum(UNIV SAO PAULO, CONJUNTO QUIMICAS, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Gezmis, Tugba; Cam, Muhammet Emin; Taskin, Duygu; Celik, Berna Ozbek; Senkardes, Ismail; Suzgec-Selcuk, SevdaIn Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones arc endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatmy and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinestemse activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.Publication Open Access Synthesis and investigation of anti-inflammatory and anticonvulsant activities of novel coumarin-diacylated hydrazide derivatives(ELSEVIER, 2020-12) ÇAM, MUHAMMET EMİN; Tapanyigit, Ozan; Demirkol, Onur; Guler, Ece; Ersatir, Mehmet; Cam, Muhammet Emin; Giray, Elife SultanA number of novel coumarin derivatives synthesized by the reaction of 3-carbonyl chloride coumarin with some substituted aryl acid hydrazides to investigate their anti-inflammatory and anticonvulsant activities. Carrageenan (0.1 ml of 1%, w/v) was injected subplantarly in the right paw of rats to induce an acute model of inflammation. Anti-inflammatory efficacy was evaluated for 5 hours at 3 different dosages 5, 10, 25 mg/kg. After that, the changes in the level of paw edema volumes and percentage inhibition of all groups were observed and the most effective coumarin derivative was found as N'-(2-hyroxybenzoyl)-2-oxo-2H-chromene-3-carbohydrazide. In addition, N'-(2-oxo-2H-chromene-3-carbonyl)nicotinohydrazide, (E)-N'-(3-(4-hydroxyphenyl)acryloyl)-2-oxo-2H-chromene-3-carbohydrazide, and N'-(5-amino-2-hydroxybenzoyl)-2-oxo-2H-chromone-3carbohydrazide showed their anti-inflammatory effects in a dose-dependent manner. On the other hand, pentylenetetrazole (PTZ, 80 mg/kg, i.p.)-induced seizure model was used to investigate the anticonvulsant activities of six newly synthesized coumarin derivatives in mice. Hybrid compound of salicylic acid hydrazide and 3-carbonyl chloride coumarin (8d) was found the most promising anticonvulsant agent among all treatment groups according to the onset of seizure and survival rate. Moreover, (E)-N'-cinnamoyl-2-oxo-2H-chromene-3-carbohyrazide (8b) and (E)-N'-(3-(4-hyroxy phenyl)acryloyl)-2-oxo-2H-chromene-3-carbohydrazide (8c) has potential anticonvulsant efficiency in low doses (30 mg/kg). The anticonvulsant effect of these coumarin derivatives may be through enhanced GABA-mediated inhibition in the brain. (C) 2020 Published by Elsevier B.V. on behalf of King Saud University.