Person:
MERTOĞLU, BÜLENT

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

MERTOĞLU

First Name

BÜLENT

Name

Filters

2006 - 20073
Reset filters

Settings

Author: ÇALLI, BARIŞ × Subject: 16S RIBOSOMAL-RNA ×

Search Results

Now showing 1 - 3 of 3
  • No Thumbnail Available
    PublicationMetadata only
    Effects of insufficient air injection on methanogenic Archaea in landfill bioreactor
    (ELSEVIER, 2007) ÇALLI, BARIŞ; Mertoglu, Bulent; Calli, Baris; Guler, Nuray; Inanc, Bulent; Inoue, Yuzo
    In this study, methanogenic Archaea diversity in an aerated landfill bioreactor filled with co-disposed incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time using molecular techniques. Besides, the effects of insufficient air injection on the bioreactor performance and methanogenic diversity were evaluated thoroughly. Results indicated that rapid bio-stabilization of solid waste are possible with aerated landfill bioreactor at various oxygen and oxidation reduction potential levels. Slot-blot hybridization results of leachate samples collected from aerated landfill bioreactor showed that archaeal and bacterial activities increased as stabilization accelerated and bacterial populations constituted almost 95% of all microorganisms. The results of slot-blot hybridization and phylogenetic analysis based on 16S rRNA gene revealed that Methanobacteriales and Methanomicrobiales were dominant species at the beginning while substituted by Methanosarcina-related methanogens close to the end of the operation of bioreactor. (c) 2006 Elsevier B.V. All rights reserved.
  • No Thumbnail Available
    PublicationMetadata only
    Evaluation of in situ ammonia removal in an aerated landfill bioreactor
    (ELSEVIER SCI LTD, 2006) ÇALLI, BARIŞ; Mertoglu, Bulent; Calli, Baris; Inanc, Bulent; Ozturk, Izzet
    The composition of nitrifying bacteria in an aerated landfill bioreactor filled with municipal solid waste incineration bottom ashes and shredded incombustible wastes was monitored and analyzed as a function of time during 1 year operation using molecular techniques. Besides, the effects of differing operational conditions on the bioreactor performance and diversity of nitrifiers were evaluated thoroughly. The results confirmed the viability of rapid aerobic bio-stabilization in an aerated landfill bioreactor operated at various ORP levels (-400 to 150 mV). BOD5 decreased faster than TOC and dropped below 10 mg/l after day 120. Subsequently, it remained quite constant until the end of the operational period. This rapid BOD5 degradation in the aerated landfill bioreactor increased the possibility of nitrification by promoting nitrifying bacteria having high oxygen affinities. The nitrification activity in the bioreactor was approved by identification of intensive amounts of Nitrosomonas-like ammonia oxidizers and Nitrospira related nitrite oxidizers with 16S rDNA and amoA based molecular microbiology techniques. (c) 2006 Elsevier Ltd. All rights reserved.
  • No Thumbnail Available
    PublicationMetadata only
    Identification of prevalent microbial communities in a municipal solid waste landfill
    (I W A PUBLISHING, 2006) ÇALLI, BARIŞ; Calli, B; Durmaz, S; Mertoglu, B
    To identify the microbial communities in Istanbul, Odayeri Municipal Solid Waste Landfill, leachate samples were collected from different sections at different stabilization phases. In identification of microbial communities in leachate samples, molecular techniques such as FISH, DGGE and cloning based on 16S rRNA and mcrA genes were used. As the chemical and microbiological compositions of the samples were compared, obvious correlations were found between the stability of the landfill section and abundance of active methanogens. On the other hand, there were considerable differences between acidogenic and mature leachate samples in DGGE profiles of archaeal and bacterial 16S rRNA genes. Moreover, in acidogenic leachate samples having BOD5/COD ratio of about 0.5 acetate utilizing Methanosarcina and Methanosaeta species were intensively detected in FISH. Although only very few H-2-utilizing methanogens were identified with FISH analysis, most of the clones isolated from mature leachate samples clustered within H-2-utilizing Methanobacteriales and Methanomicrobiales according to phylogenetic analysis of 16S rRNA and mcrA clones, respectively.