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BİTİŞ, LEYLA

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2017 - 201922020 - 20221
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Author: ŞEN, ALİ × Subject: Asteraceae ×

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Now showing 1 - 3 of 3
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    PublicationOpen Access
    In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.
    (2022-09-01) YILDIRIM, AYBENİZ; ŞEN, ALİ; ŞENKARDEŞ, İSMAİL; BİTİŞ, LEYLA; Yıldırım A., ŞEN A., HACIOĞLU M., BİRTEKSÖZ TAN A. S. , ŞENKARDEŞ İ., BİTİŞ L.
    In this study, in vitro biological activities and total phenol/flavonoid contents of methanol extract (ISM) and its hexane (ISH), chloroform (ISC), ethyl acetate (ISEA) and aqueous methanol (ISAM) fractions obtained from aerial parts of Inula salicina were investigated. ISEA showed the highest antioxidant activity against DPPH and ABTS radicals with an IC50 value of 0.014 mg ml-1 for both assays. ISEA exhibited a good anti-inflammatory activity with an IC50 value of 0.060 mg ml-1 . ISEA was found to exhibit a moderate level of antidiabetic activity against α amylase enzyme with an IC50 value of 0.290 mg ml-1 . ISEA and ISM presented low and moderate inhibitory activity against acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 0.577 and 0.279 mg ml-1 , respectively. ISC with MIC values of 78 and 156 µg ml-1 displayed a significant antimicrobial activity against Staphylococcus aureus and S. epidermidis, respectively. Almost all extracts had moderate effect against Candida species. The highest total phenolic and flavonoid contents were determined in ISEA with 574.8 mg GAE (gallic acid equivalent) g -1 extract and 30.48 mg QE (quercetin equivalent) g -1 extract, respectively. These results showed that ISEA had a good antioxidant and anti-inflammatory activity with moderate α-amylase and butyrylcholinesterase inhibitory activity. Also, ISC exhibited a significant antimicrobial activity against Staphylococcus species.
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    PublicationOpen Access
    Biological activities of aerial parts extracts of endemic Tanacetum argenteum subsp. argenteum
    (MARMARA UNIV, FAC PHARMACY, 2017-04-01) ŞEN, ALİ; Sen, Ali; Ozakpinar, Ozlem Bingol; Tan, Seher Birteksoz; Kultur, Sukran; Uras, Fikriye; Bitis, Leyla
    The objective of the present study was to investigate the antimicrobial and anti-proliferative activities of n-hexane, chloroform, methanol and aqueous methanol extracts obtained from Tanacetum argenteum subsp. argenteum aerial parts. Anti-proliferative activity was tested in vitro against four human cancer cell lines (A549: lung adenocarcinoma, Hela; cervix adenocarcinoma, HT-29: colon adenocarcinoma, MCF-7; breast adenocarcinoma) using MTT assay. Antimicrobial activity was assessed by micro-broth dilution technique against Staphylococcus aureus ATCC 29213, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Klebsiella pneumoniae ATCC 4352, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 14153, and Candida albicans ATCC 10231. Hexane extract inhibited proliferation of HT-29 and MCF-7 by 75 and 74% while chloroform extract against the same cancer cell lines displayed inhibition of 89 and 73% at the concentration of 30 mu g/mL, respectively. Also, chloroform extract at the same concentration showed significant anti-proliferative activity against A-549 and HeLa with inhibition values of 75% and 62%. Chloroform extract exhibited moderate antibacterial activity against Staphylococcus aureus and S. epidermidis with the MIC values of 625 mu g/mL. Methanol and aqueous methanol extracts showed weak antimicrobial activity against Staphylococcus epidermidis and Candida albicans with MIC values of 1250 mu g/mL. The results showed that n-hexane and chloroform extracts have significant anticancer activity against cancer cell lines used in this study.
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    PublicationOpen Access
    Bioactivity-guided isolation of anti-proliferative compounds from endemic Centaurea kilaea
    (TAYLOR & FRANCIS LTD, 2017-01-01) ŞEN, ALİ; Sen, Ali; Turan, Suna Ozbas; Bitis, Leyla
    Context: The genus Centaurea L. (Asteraceae) is one of the largest genera in Turkey. Compounds and extracts obtained from different Centaurea species have significant anti-cancer activity against various cancer cell lines. Objective: To determine the anti-proliferative activity of isolates from the chloroform extract of C. kilaea Boiss. Materials and methods: Eleven compounds were isolated using column chromatography and preparative TLC from the chloroform extract of aerial parts of endemic C. kilaea. The structures of the isolated compounds were elucidated by various spectroscopic methods, including UV, H-1-NMR and C-13-NMR. Anti-proliferative activity of compounds (0.5-50 mu g/mL) were measured against one normal cell line (L-929, mouse fibroblast) and three human cancer cell lines (Hela, cervix carcinoma; MCF-7, breast carcinoma; PC-3, prostate carcinoma) using MTT assay. Results were expressed as IC50 values. Results: None of the 11 compounds displayed activity against L-929 and HeLa. Two of these compounds, cnicin and cirsimaritin, showed fairly strong activity against MCF-7 and PC-3 with IC50 values of 3.25 and 4.3 mu g/mL, respectively. Discussion and conclusion: This is the first report on cirsimaritin. Cirsimaritin and cnicin could serve as potential anti-cancer drug candidates against breast and prostate cancer, respectively.