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BİTİŞ, LEYLA

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LEYLA

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Has files: true × Subject: Genel Farmakoloji, Toksikoloji ve Eczacılık ×

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    PublicationOpen Access
    The effects of Origanum onites in streptozotocin-induced diabetes mellitus in rats
    (2022-01-01) POLAT, ELİF BEYZANUR; TAŞKIN, TURGUT; BİTİŞ, LEYLA; ELÇİOĞLU, HATİCE KÜBRA; Aydemir O., Polat E. B. , Aljesri K., TAŞKIN T., BİTİŞ L., ELÇİOĞLU H. K.
    © 2022 Marmara University Press.Diabetes mellitus is a chronic disease characterized by decreased insulin synthesis and/or increased blood glucose due to insulin resistance. In this study, streptozotocin (STZ)-induced diabetic rats were evaluated and it was aimed to investigate the protective effects of Origanum onites L. (OO) against possible changes in these parameters. Male Sprague-Dawley rats weighing 300-400g were divided into three groups control (C), diabetes mellitus (DM) and diabetes mellitus + OO (OO group). DM was induced by administration of STZ 60 mg/kg dose intraperitoneally (i.p.) after 48 hours, rats with blood glucose values higher than 200 mg/dL were considered DM. Origanum extract was administered i.p. to the OO group at 50 mg/kg per day for 6 weeks. Serum AST, ALT, creatinine, and inflammatory cytokine levels were measured. MDA and GSH levels were measured in liver tissues. AST, ALT, creatinine, and MDA levels were found to be increased in the DM group, while a significant decrease in these levels was observed in the treated group. While GSH values fell in the DM group, a significant increase occurred in the OO group (n=6, p<0.0001; two-way ANOVA). When the plasma levels of cytokines were examined, an increase was observed in the DM group and a significant decrease was observed in the OO group. When we evaluate our findings, we think that OO has a protective effect against complications that may occur in DM by preventing oxidant damage and inflammation. Further studies are needed on the protective effects of OO in DM.
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    PublicationOpen Access
    LC-MS/MS analysis and biological activities of endemic Achillea sieheana Stapf from Türkiye
    (2022-01-01) DİKPINAR, TUĞÇE; BİTİŞ, LEYLA; DİKPINAR T., GÖGER F., BİTİŞ L.
    © 2022 Marmara University Press.In this study, in vitro ABTS, DPPH, 5-lipoxygenase, and α-glucosidase inhibitory activities of n-hexane (ASH), dichloromethane (ASD), ethyl acetate (ASE), and methanol (ASM) extracts from the aerial parts of endemic A. sieheana were assayed for the first time. The phytochemical content of ASE, the most active extract, has been first determined by LC-MS/MS. Also, the total amount of phenolic compounds of extracts were calculated. ASE demonstrated significant antioxidant activity with IC50 values of 0.096 and 0.156 mg/mL for ABTS and DPPH, respectively. ASD and ASE with IC50 values of 0.045 and 0.089 mg/mL exhibited significant anti-inflammatory activity. ASE showed moderate α-glucosidase inhibitory activity with an IC50 value of 0.774 mg/mL. Also, the total phenolic content was found to be highest in the ASE. Feruloylquinic acid, luteolin, luteolin glucoside, isorhamnetin, isovitexin, methoxyflavonoid like chrysoeriol and its glycoside derivative in the ASE were detected by LC-MS/MS. The results demonstrated that ASE had significant anti-inflammatory, antioxidant and moderate α-glucosidase inhibitory potential.