Person:
BİTİŞ, LEYLA

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

BİTİŞ

First Name

LEYLA

Name

Filters

2018 - 201912020 - 20242
Reset filters

Settings

Has files: true × Subject: anti-inflammatory ×

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    PublicationOpen Access
    Antioxidant and anti-inflammatory activities of Phlomis pungens and Coridothymus capitatus
    (MARMARA UNIV, FAC PHARMACY, 2018-01-12) YAVUZ, AYŞE NUR; Taskin, Turgut; Cam, Muhammet Emin; Bulut, Gizem; Hazar-Yavuz, Ayse Nur; Kabasakal, Levent; Bitis, Leyla
    The purpose of this study was to comparatively reveal for the first time the antioxidant, anti-inflammatory effects of Phlomis pungens and Coridothymus capitatus methanol extracts obtained by using maceration extraction method. According to the results of anti-inflammatory activity, P. pungens methanolic extract demonstrated a more prominent and intensive antiinflammatory effect with 24.7% of inhibitive capacity in the altered edema size after the first hour of carrageenan injection compared to C. capitatus methanolic extract. P. pungens methanolic extract inhibitory effect increased during three hours and reached maximum by 41.9%. According to the results obtained from antioxidant activity experiments, methanol extract of C. capitatus exhibited stronger free radical scavenging (DPPH), cupric reducing (CUPRAC), ferric reducing/antioxidant power (FRAP) and ABTS radical cation scavenging activity than P. pungens extract. In additon, C. capitatus extract had higher ABTS radical cation scavenging and ferric reducing/antioxidant power activity than ascorbic acid, BHT and BHA, respectively. Therefore, this extract can be used in both medicine and food industry as a natural antioxidant source.
  • Thumbnail Image
    PublicationOpen Access
    LC-MS/MS analysis and biological activities of endemic Achillea sieheana Stapf from Türkiye
    (2022-01-01) DİKPINAR, TUĞÇE; BİTİŞ, LEYLA; DİKPINAR T., GÖGER F., BİTİŞ L.
    © 2022 Marmara University Press.In this study, in vitro ABTS, DPPH, 5-lipoxygenase, and α-glucosidase inhibitory activities of n-hexane (ASH), dichloromethane (ASD), ethyl acetate (ASE), and methanol (ASM) extracts from the aerial parts of endemic A. sieheana were assayed for the first time. The phytochemical content of ASE, the most active extract, has been first determined by LC-MS/MS. Also, the total amount of phenolic compounds of extracts were calculated. ASE demonstrated significant antioxidant activity with IC50 values of 0.096 and 0.156 mg/mL for ABTS and DPPH, respectively. ASD and ASE with IC50 values of 0.045 and 0.089 mg/mL exhibited significant anti-inflammatory activity. ASE showed moderate α-glucosidase inhibitory activity with an IC50 value of 0.774 mg/mL. Also, the total phenolic content was found to be highest in the ASE. Feruloylquinic acid, luteolin, luteolin glucoside, isorhamnetin, isovitexin, methoxyflavonoid like chrysoeriol and its glycoside derivative in the ASE were detected by LC-MS/MS. The results demonstrated that ASE had significant anti-inflammatory, antioxidant and moderate α-glucosidase inhibitory potential.
  • Thumbnail Image
    PublicationOpen Access
    Anti-inflammatory compounds and a new sesquiterpene lactone from Centaurea gabrieljanae Greuter
    (2024-01-01) ŞEN, ALİ; BİTİŞ, LEYLA; Yıldırım A., ŞEN A., BİTİŞ L.
    The traditional use of Centaurea spp. for anti-inflammatory purposes is widespread among the people in Turkiye. For this, the methanol extract of Centaurea gabrieljanae and sub-fractions of the methanol extract were tested for anti-inflammatory activity using 5-LOX, while their antioxidant activities, total phenol, and total flavonoid contents were also examined. The ethyl acetate fraction exhibited potent anti-inflammatory activity (IC50 = 3.864 ± 0.9 µg/ml), from which five known compounds (astragalin, picein, p-hydroxy benzoic acid, 3,4-dimethoxy-cinnamic acid, 4-hydroxybenzoic acid 4-O-β-glucopyranoside) and a new sesquiterpene lactone named Pterochlorin were obtained. Pterochlorin showed potent anti-inflammatory activity with a value of IC50 12.71 ± 0.7 µg/ml compared to standard indomethacin. Similarly, astragalin was found to be strong (IC50 = 18.23 µg/ml). In addition, 4-hydroxybenzoic acid 4-O-β-glucopyranoside was isolated for the first time in Centaurea species, and its anti-inflammatory activity was tested. This study may be a guide for the discovery of a new anti-inflammatory drug derived from natural sources.