Person: FİLİNTE, DENİZ
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FİLİNTE
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DENİZ
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Publication Metadata only Mesane kanseri tanısında kullanılmak üzere üriner protein ve ekzozomal mikro RNA belirteci saptanması(2019-10-27) FİLİNTE, DENİZ; PEKER EYÜBOĞLU, İREM; GÜLLÜ AMURAN G., TİNAY İ., ILGIN C., FİLİNTE D., PEKER EYÜBOĞLU İ.Publication Metadata only Suppressive effect of compact bone-derived mesenchymal stem cells on chronic airway remodeling in murine model of asthma(ELSEVIER SCIENCE BV, 2014) FİLİNTE, DENİZ; Ogulur, Ismail; Gurhan, Gulben; Aksoy, Ayca; Duruksu, Gokhan; Inci, Cigdem; Filinte, Deniz; Kombak, Faruk Erdem; Karaoz, Erdal; Akkoc, TuncNew therapeutic strategies are needed in the treatment of asthma besides vaccines and pharmacotherapies. For the development of novel therapies, the use of mesenchymal stem cells (MSCs) is a promising approach in regenerative medicine. Delivery of compact bone (CB) derived MSCs to the injured lungs is an alternative treatment strategy for chronic asthma. In this study, we aimed to isolate highly enriched population of MSCs from mouse CB with regenerative capacity, and to investigate the impact of these cells in airway remodeling and inflammation in experimental ovalbumin-induced mouse model of chronic asthma. mCB-MSCs were isolated, characterized, labeled with GFP and then transferred into mice with chronic asthma developed by ovalbumin (OVA) provocation. Histopathological changes including basement membrane, epithelium, subepithelial smooth thickness and goblet cell hyperplasia, and MSCs migration to lung tissues were evaluated. These histopathological alterations were increased in ovalbumin-treated mice compared to PBS group (P < 0.001). Intravenous administration of mCB-MSC significantly reduced these histopathological changes in both distal and proximal airways (P < 0.001). We showed that GFP-labeled MSCs were located in the lungs of OVA group 2 weeks after intravenous induction. mCB-MSCs also significantly promoted Treg response in ovalbumin-treated mice (OVA + MSC group) (P < 0.037). Our studies revealed that mCB-MSCs migrated to lung tissue and suppressed histopathological changes in murine model of asthma. The results reported here provided evidence that mCB-MSCs may be an alternative strategy for the treatment of remodeling and inflammation associated with chronic asthma. (C) 2014 Elsevier B.V. All rights reserved.Publication Metadata only Increasing the survival of transverse rectus abdominis musculocutaneous flaps with a Botulinum toxin-A injection: A comparison of surgical and chemical flap delay methods(ELSEVIER SCI LTD, 2016) FİLİNTE, DENİZ; Temiz, Gokhan; Yesiloglu, Nebil; Sirinoglu, Hakan; Akpinar, Ali Cem; Sarici, Murat; Filinte, Deniz; Filinte, Gaye Taylan; Bozkurt, MehmetBackground: Botulinum toxin type-A (Bot-A) is a commonly used drug for both cosmetic and therapeutic purposes. The effects of Bot-A on skin and muscle flaps and the related mechanisms have been described previously. In this study, we used a rat transverse rectus abdominis musculocutaneous (TRAM) flap model to examine the effects of Bot-A on the skin island, which is perfused by the rectus abdominis muscle according to the angiosome concept. Methods: Forty female rats were divided into five groups, including control and sham groups. In the control group, a TRAM flap was raised and sutured back after inserting a silicone sheath underneath the flap. In the sham group, the flap was raised 1 month after injecting saline into the muscle. In the chemical delay group, the flap was raised 1 month after injecting 10 IU of Bot-A. In the surgical delay group, the flap was raised 2 weeks after ligating the cranial epigastric artery. In the surgical and chemical delay group, a Bot-A injection was performed initially, a cranial epigastric artery was ligated after 2 weeks, and a TRAM flap was raised after the first month. In all groups, laser Doppler examination, photographic documentation, and analysis of the flap survival rates were performed. In the histopathological evaluation, the diameter measurements of the caudal epigastric vessels, vascular density measurements using CD31 stain, and apoptotic rate estimation using the Tunnel method were performed. Results: The necrosis ratios, arterial cross-sectional diameters, and microvascular density measurements were significantly superior compared to those of control and sham groups; however, there was no significant difference between the delay groups. There was also no difference in the laser Doppler measurements between the groups and the zones of the TRAM flaps. Conclusion: An injection of Bot-A increases muscular circulation and flap survival of TRAM flaps in rats. (C) 2016 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.Publication Open Access The Intensity of PLA2R and C4d_x000D_ Immunoexpression in Primary_x000D_ Membranous Nephropathy(2019) FİLİNTE, DENİZ; Deniz FİLİNTE;Hakkı ARIKAN;Mehmet KOÇ;Handan KAYA;İshak Çetin ÖZENER;Gamze AKBAŞObjective: Antibodies against the phospholipase A2 receptor (PLA2R) on podocyte membranes result in the formation of immune complexes that cause loss of function of theglomerular basement membrane in primary membranous nephropathy (PMN). It has alsobeen demonstrated that there is a deposition of complement 4d (C4d) in the glomeruli inPMN. The present study aims to evaluate PLA2R and C4d immunoexpressions in PMN casesand search the correlation with the clinical parameters.Methods: In this study, clinicopathological data and paraffin-embedded specimens werecollected from 51 patients. The formalin-fixed paraffin-embedded tissues were stained usingroutine hematoxylin-eosin, periodic acid-Schiff, and silver methenamine stains and immunostained for anti-PLA2R and C4d. Ten normal kidney tissues and 10 focal segmental glomerulosclerosis (FSGS) cases were selected as controls for PLA2R and C4d immunoexpression.Results: Of the PMN cases, 51 (100%) cases were positive for PLA2R, including 15 (29%)cases that scored 2+, and 36 (71%) cases that scored 3+. Forty of the 51 cases (78%) werepositive for C4d. The percentages of cases staining positively for C4d, per scoring group,were as follows: 31 (61%) cases faintly (1+) positive and 9 (18%) cases moderately (2+)positive. No strong positivity was observed. All of the control cases (100%) were negativefor PLA2R and C4d. There was no statistically significant difference between the intensity ofthe staining of PLA2R and the staining of C4d, proteinuria levels, creatinine levels, and complement 3 (C3) positivity. Similarly, there was no statistically significant difference betweenthe intensity of the staining of C4d and proteinuria levels, creatinine levels, and C3 positivity.Conclusion: Immunohistochemical detection of PLA2R and C4d is a safe and easy methodfor the diagnosis of PMN. In cases where fresh tissue is not available for the detection of IgGand C3 using the immunofluorescence method, positivity for PLA2R and C4d with immunohistochemistry may be beneficial for the diagnosis of PMN.Publication Metadata only Allogeneic pluripotent stem cells suppress airway inflammation in murine model of acute asthma(ELSEVIER, 2014) FİLİNTE, DENİZ; Ogulur, Ismail; Gurhan, Gulben; Kombak, Faruk Erdem; Filinte, Deniz; Barlan, Isil; Akkoc, TuncNew strategies are needed to suppress airway inflammation and prevent or reverse airway remodeling in asthma. Reprogramming induced pluripotent stem cells (iPSCs) have the potential of embryonic stem cells (ESCs) and provide a resource for stem cell-based utility. The aim of this study was to evaluate the histopathological and immunomodulatory effects of ESCs and iPSCs for potential allogenic application in a murine model of acute asthma. BALB/c mice were sensitized with alum-absorbed ovalbumin (OVA) and then challenged with 1% aerosolized OVA. 5 x 10(5) ESCs and iPSCs were administrated intranasally on the last day of nebulization. Mice were sacrificed after 24 h, and serum allergen specific antibody level, airway remodeling, cytokine levels in lung supernatants, and eosinophilic infiltration in BAL fluid were examined. As a result, more ESCs and iPSCs integrated into the lungs of mice in OVA groups than those of the controls. Epithelial, smooth muscle and basal membrane thicknesses as well as goblet cell hyperplasia occurring in airway remodeling were significantly suppressed by pluripotent stem cells in both distal and proximal airways. Percentage of eosinophils decreased significantly in BAL fluid as well as serum allergen-specific IgE and IL-4 levels in lung supernatants. On the contrary, regulatory cytokine - IL-10 level - was enhanced. Application of especially ESCs significantly increased the percentage of Treg subsets. Our comparative results showed that i.n. delivery of miRNA-based reprogrammed iPSCs is beneficial in attenuating airway inflammation in a murine model of acute asthma, and that cells also have similar immunomodulatory effects in mice. (C) 2014 Elsevier B.V. All rights reserved.Publication Metadata only Effects of Different Concentrations of Injectable Collagenase Enzyme on Capsular Tissue Around Silicone Implants: A Preliminary Experimental Study for the Development of a New Treatment Strategy(SPRINGER, 2016) FİLİNTE, DENİZ; Yesiloglu, Nebil; Temiz, Gokhan; Sarici, Murat; Yildiz, Kemalettin; Sirinoglu, Hakan; Guvercin, Emre; Akpinar, Ali Cem; Filinte, Gaye Taylan; Filinte, DenizIn recent studies, collagen organization was blamed for the formation of capsular contracture which is still a challenging problem after silicone implant-based breast operations. In this study, effects of different concentrations of collagenase enzyme derived from Clostridium histolyticum on the capsular tissue formation around the silicone implants were investigated. The injectable form of collagenase has a routine clinical use in the treatment of both Dupuytren's and Peyronie's diseases. Thirty-two Wistar albino rats were randomized into four groups. A 2 x 1 x 0.3-cm-sized silicone block was inserted inside a dorsal subcutaneous pocket in all groups. After 2 months of insertion, capsule thicknesses around the implants were detected under ultrasonography. This was followed by injection of isotonic saline, 150, 300, and 600 IU in Gr-1, 2, 3, and 4, respectively. All the animals were sacrificed at the end of the first week for histologic sampling to determine fibroblast proliferation, vessel density of the tissue, necrosis, edema, inflammation, and capsule thickness. All the data were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests and compared for significance of the results. There was no significant difference in terms of capsule thinning between the 300 and 600 IU groups but in both groups thinning was significantly higher than the sham group. In the 150 IU group there was no significant thinning as compared to the sham group (p > 0.05). However, complications such as skin necrosis, infection, and seroma formation were seen only in the 600 IU injection group. The optimal safe and effective dose of the enzyme was accepted as 300 IU. The 300 IU injection provided up to 89 % thinning in the capsule tissue. There was thinning of the collagen bundles parallel to capsule thickness. In the 600 IU group, micro-pores were encountered at the thinnest points. However, the late results and recurrence rates of capsular contracture were not included in this study; collagenase seemed effective for the reduction of capsular tissue around the implants. This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors http://www.springer.com/00266.Publication Metadata only Jukstaglomerüler hücrelı̇ tümör (renı̇noma): Olgu bildirimi(2015-11-28) ŞENER, TARIK EMRE; TANIDIR, YILÖREN; ŞAHİN, BAHADIR; CİNEL, ZELİHA LEYLA; FİLİNTE, DENİZ; ŞENER T. E., Sekerci C. A., TANIDIR Y., ŞAHİN B., CİNEL Z. L., FİLİNTE D., Önem İ., AKBAL C.Giriş Reninoma, jukstaglomerüler hücrelerden köken alan ve nadir görülen bir tümördür. İlk olarak 1967 yılında Robertson ve ark. tarafından bildirilen reninoma olgusundan sonra dünya üzerinde bugüne kadar 100 kadar olgu bildirilmiştir. Gereç-Yöntem Bu bildiride polikliniğimize antihipertansif tedavi ile kontrol altında olan sekonder hipertansiyonu ve yan ağrısı ile başvuran 15 yaşında bir erkek hastada saptanan reninoma olgusunu bildiriyoruz. Bulgular Hastanın yapılan ilk değerlendirmesinde hipokalemik olduğu saptandı ve yapılan kontrastlı tüm batın MR görüntülemesinde kontrast tutan 4,8x4,8 cm büyüklüğünde egzofitik solid lezyon olduğu görüldü. Hastaya açık nefron koruyucu cerrahi uygulandı ve kitle güvenli cerrahi sınır ile çıkarıldı. Yapılan patolojik inceleme tümörün jukstaglomerüler hücreli tümör (Reninoma) olduğunu gösterdi. Hastanın postoperatif takiplerinde antihipertansif ilaçları kesildi ve hastanın tansiyon değerleri normal seviyelere döndü ve biyokimyasal incelemelerde hipokalemisin ortadan kalktığı ve normal potasyum değerlerine ulaştığı görüldü. Sonuç Bu olgu çocuklarda nadir de olsa sekonder hipertansiyonun etyolojisi arasında bulunan reninomanın ayırıcı tanıda düşünülmesinin önemini vurgulamaktadır.Publication Metadata only The Effects of Different Amounts of Thrombin Application on Fat Graft Viability in Rats: An Experimental Study(WOLTERS KLUWER MEDKNOW PUBLICATIONS, 2019) FİLİNTE, DENİZ; Kacmaz, Caner; Gideroglu, Kaan; Guvercin, Emre; Filinte, Gaye Taylan; Bozkurt, Mehmet; Filinte, DenizAims: The most important disadvantage of fat graft, which is also a late-term complication, is graft resorption. The aim of this study is to evaluate the effect of thrombin, which is reported to increase the tissue regeneration and angiogenesis in many areas, to viability of fat graft. Settings and Design: Twenty Wistar-Albino type adult male rats were used in the study. 'they were divided into four groups as one control group and three experimental group. Subjects and Methods: Inguinal fat pads were excised and reduced to 500 mg (+/- 1 mg) in all animals. To obtain thrombin, 3 animals were sacrificed. One percent amount of 0.25 mg lidocaine hydrochloride was injected to the scapular regions of the animals. Afterward, subcutaneous cavities were formed there to place the fat tissue inside. After placing the graft, varying proportions of thrombin were injected to the animals in the experimental groups. No thrombin was not injected to the control group. After 90 days, the experimental animals were sacrificed, and the fat grafts were removed. Statistical Analysis Used: The data obtained from control and experimental groups were analyzed using SPSS software (version 20.0; SPSS Inc., Chicago, IL, USA). Normal fat ratio, cyst or vacuole development, inflammation, fibrosis, microvascular density, apoptosis, and weight score differences between groups were compared using Kruskal-Wallis test. To compare two groups with different scores, Mann-Whitney U test was used. The statistical significance level was accepted as 0.05 (P = 0.05). Results: Macroscopic, histological, and statistical evaluations showed that thrombin has reduced the weight and volume loss on fat graft, increased viable fat cell amount and reduced inflammation on receptive area. Conclusion: The positive effects of thrombin on the viability of fat graft have given us courage to use it in further studies. Longer follow-ups are necessary and more studies are required to use it in clinical practice in combination with fat grafts.Publication Metadata only Mouse Bone Marrow Derived Mesenchymal Stem Cells Supress Airway Inflammation In Both Chronic and Acute Murine Asthma Model(MOSBY-ELSEVIER, 2014) FİLİNTE, DENİZ; Akkoc, Tunc; Ogulur, Ismail; Gurhan, Gulben; Aksoy, Ayca; Duruksu, Gokhan; Filinte, Deniz; Kombak, Erdem; Barlan, Isil B.; Karaoz, ErdalPublication Open Access The Determination of Normal Percentages of Syncytiotrophoblastic Knots in Various Regions of Placenta: Where to Count the Syncytial Knots(DE GRUYTER POLAND SP ZOO, 2014) FİLİNTE, DENİZ; Cigercuigykkaru, Engin; Filinte, Deniz; Toz, Emrah; Avci, Ibrahim; Erdem, Baki; Eminli, Itibar; Ozgur, TanerObjective: The marginal, basal and subchorial regions of the placenta are considered to be more hypoxic than other regions. Therefore, it is not recommended to determine the increase in syncytiotrophoblast knots, based on the major morphological change in placental hypoxia, from the samples taken from these regions. However, the normal count of knots at various regions of placenta is not investigated. Material and Method: In this study we have sampled morphologically and clinically normal placenta with eccentric cord insertion from various sites, either close to cord entrance or away from it (marginal, non-marginal basal, non-marginal subchorial, and nonmarginal midparanchymal). The number of knots was calculated on a total of at least 100 villi for each placental sample. The normal amount of knots in different regions and comparison between them were investigated. Twenty-eight placentas with eccentric cord insertion were sampled in the same manner. Hot spots from the above mentioned regions were counted in a total of 100 villi. Results: No significant difference was found between the dual comparison of the mean percentages of different regions (p: 0.148). The variety of hypoxia in different regions of the placenta could not be demonstrated in this study. Conclusion: It is found that there is no difference in perfusion that can be morphologically demonstrated with increase in syncytiotrophoblast knot, between different regions of placenta.