Person:
SARIYAR AKBULUT, BERNA

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

SARIYAR AKBULUT

First Name

BERNA

Name

Filters

20151
Reset filters

Settings

Author: BULDUM, GİZEM ×

Search Results

Now showing 1 - 1 of 1
  • No Thumbnail Available
    PublicationMetadata only
    Investigation of the in vivo interaction between beta-lactamase and its inhibitor protein
    (TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2015) SARIYAR AKBULUT, BERNA; Budeyri Gokgoz, Nilay; Yalaz, Simay; Avci, Naze Gul; Buldum, Gizem; Ozkirimli Olmez, Elif; Sariyar Akbulut, Berna
    The affinity of beta-lactamase inhibitory protein (BLIP) for TEM-1 beta-lactamase has raised hopes in the challenge of protein-based inhibitor discovery for beta-lactamase-mediated antibiotic resistance. Currently, the effect of the formation of the beta-lactamase:BLIP complex in vivo in beta-lactam resistant bacteria is an open question. The scarcity of information to the extent to which BLIP can impair beta-lactamase activity inside cells has urged us to assess the in vivo efficacy of BLIP as a potent beta-lactamase inhibitor. To this end, beta-lactamase and BLIP were coexpressed in Escherichia coli. Simultaneous expression of beta-lactamase and BLIP and the formation of the TEM-1 beta-lactamase: BLIP complex in the periplasmic space of E. coli were verified by electrophoretic and Western blot techniques. Growth profiles of the cells expressing both beta-lactamase and its protein inhibitor, complemented with beta-lactamase activity measurements, suggested that BLIP synthesis retarded cell growth and reduced beta-lactamase activity. Although co-expression of beta-lactamase and its protein inhibitor did not completely impair cell growth, the specificity of BLIP enabled it to bind beta-lactamase in the bacterial periplasm, regardless of the crowding components.