Person: SARIYAR AKBULUT, BERNA
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SARIYAR AKBULUT
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Publication Metadata only Retro-techno-economic evaluation of acetic acid production using cotton stalk as feedstock(SPRINGER, 2018) SAYAR, AHMET ALP; Sayar, Nihat Alpagu; Kazan, Dilek; Pinar, Orkun; Akbulut, Berna Sariyar; Sayar, Ahmet AlpIn value-added chemical industries, use of agricultural wastes as raw materials remains to be a major challenge in commercialization due to lack of competitiveness with respect to petrochemical processes. This work presents the techno-economic analysis of a novel bioprocessing plant converting 356,400MT/year cotton stalks into 147,000MT/year acetic acid. A production scheme integrating lignin separation with the main bioconversion stages has been proposed. Techno-economic assessment was performed through economic feasibility and retro-techno-economic analysis (RTEA) methods. The RTEA method has been extended to include the estimation of research and development funding for improving economic feasibility. Carbon offset of the proposed technology has been estimated and carbon credit results have been incorporated into the economic feasibility metrics.Publication Open Access A two-step purification platform for efficient removal of Fab-related impurities: A case study for Ranibizumab(2023-11-01) PİNAR, ORKUN; SARIYAR AKBULUT, BERNA; KAZAN, DİLEK; Tatli O., Oz Y., Dingiloglu B., Yalcinkaya D., Basturk E., Korkmaz M., Akbulut L., Hatipoglu D., Kirmacoglu C., Akgun B., et al.Antibodies (mAbs) and antibody fragments (Fabs) constitute one of the largest and most rapidly expanding groups of protein pharmaceuticals. In particular, antibody fragments have certain advantages over mAbs in some therapeutic settings. However, due to their greater chemical diversity, they are more challenging to purify for large-scale production using a standard purification platform. Besides, the removal of Fab-related byproducts poses a difficult purification challenge. Alternative Fab purification platforms could expedite their commercialization and reduce the cost and time invested. Accordingly, we employed a strong cation exchanger using a pH-based, highly linear gradient elution mode following Protein L affinity purification and developed a robust two-step purification platform for an antibody fragment. The optimized pH gradient elution conditions were determined on the basis of purity level, yield, and the abundance of Fab-related impurities, particularly free light chain. The purified Fab molecule Ranibizumab possessed a high degree of similarity to its originator Lucentis. The developed purification platform highly intensified the process and provided successful clearance of formulated Fab- and process-related impurities (∼98 %) with an overall process recovery of 50 % and, thus, might be a new option for Fab purification for both academic and industrial purposes.Publication Metadata only Production of industrial enzymes for feed industry(ELSEVIER SCIENCE BV, 2012) SARIYAR AKBULUT, BERNA; Sogut, Melis S.; Ozturk, Nurcin C.; Ozturk, H. Umit; Pinar, Orkun; Ipek, Fatih M.; Cayir, Ebru; Gokce, Abdulmecit; Karasu, Deniz; Ozturk, Dilek C.; Ozturk, Yavuz; Denizci, A. Akin; Ozturk, Bengu; Bahar, Banu; Avci, Naze G.; Ayan, Burak; Kazan, Dilek; Duman, Yonca A.; Sargin, Sayit; Karacanli, Sevket; Mizrak, Cengizhan; Catli, A. Ugur; Kucukyilmaz, Kamil; Guvenmez, Hatice K.; Arikan, Burhan; Bozkurt, Mehmet; Seyhan, Ferda; Akbulut, Berna S.; Erarslan, A. Altan; Ongen, Gaye; Dincer, Sadik; Yenice, Engin; Mandaci, SevnurPublication Metadata only Techno-economic analysis of caffeine and catechins production from black tea waste(ELSEVIER, 2019) SARIYAR AKBULUT, BERNA; Sayar, Nihat Alpagu; Sam, Selcen Durmaz; Pinar, Orkun; Serper, Damla; Akbulut, Berna Sanyar; Kazan, Dilek; Sayar, Ahmet Alpy This work establishes a proof of concept level economic assessment of a novel commercial production scheme for caffeine and catechins from black tea waste collected in the north-eastern region of Turkey. A base case in which dichloromethane (DCM) is used as the product extracting agent and an alternative greener case where DCM is replaced with environmentally friendlier ethyl acetate (EA) were designed and modelled on Superpro Designer platform. Profitability analyses were conducted for both cases, each treating 30,000 MT/year of black tea waste. Under the base case scenario, the Net Present Value (NPV) was calculated as 16,249,000 USD whereas the alternative scenario gave an NPV of 4,379,000 USD with corresponding payback times of 5.64 and 6.79 years. A novel techno-economic evaluation method involving the metamodeling of Superpro Designer models via Kriging and subsequent multi-objective optimization is incorporated into the economic assessment and applied to the two cases in order to optimize selected dominant process variables. Optimal values for dominant operating process variables were found using 3-D graphs simulating the combined effects of these variables on NPV. Results showed that the base case has a greater potentiality to generate profit. However, the greener case could be implemented if it is supported on environmental and toxicity issues considering the potential health and pollution preventive benefits. The present work demonstrates how the integration of novel conceptual design, modelling, and optimization approaches can foster broader evaluation of bioprocesses utilizing negative-value agro-wastes for the production of value-added chemicals. (C) 2019 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.