Person: AKAKIN, DİLEK
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AKAKIN
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DİLEK
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Publication Metadata only Apigenin’in hafif travmatik beyin hasarı modelinde olası antienflamatuar, antioksidan ve nöroprotektif etkisinin incelenmesi(2021-11-21) AKAKIN, DİLEK; PEKER EYÜBOĞLU, İREM; YILDIRIM, ALPER; YÜKSEL, MERAL; KURU BEKTAŞOĞLU P., DEMİR D., KOYUNCUOĞLU T., YÜKSEL M., KARAGÖZ KÖROĞLU A., AKAKIN D., PEKER EYÜBOĞLU İ., YILDIRIM A., ÇELİKOĞLU E., GÜRER B.Publication Metadata only Bacterial cellulose tubes as a nerve conduit for repairing complete facial nerve transection in a rat model(SPRINGER, 2020) BİNNETOĞLU, ADEM; Binnetoglu, Adem; Demir, Berat; Akakin, Dilek; Demirci, Elif Kervancioglu; Batman, CaglarPurpose Functionality of the facial nerve is cosmetically important. While many techniques have been investigated, early and effective treatment for traumatic facial nerve paralysis remains challenging. Here, we aim to examine bacterial cellulose (BC) as a new tubularization material for improving facial nerve regeneration. Methods Our study was performed on 40 female Sprague Dawley rats. Rats were randomly divided into four groups, with 10 rats per group. In all rats, the main trunk of the facial nerve was completely cut 8 mm before the branching point. For repairing the facial nerve, in group 1, the nerve was left to recover spontaneously (control group); in group 2, it was repaired by primary suturing (8.0 Ethilon sutures, Ethicon); in group 3, BC tubes alone were used to aid nerve repair; and in group 4, both BC tubes and primary sutures (8.0 Ethilon sutures) were used. After 10 weeks, the facial nerve regeneration was evaluated by the whisker movement test and electrophysiologically (nerve stimulation threshold and compound muscle action potential). Nerve regeneration was assessed by calculating the number of myelinated nerve fibers, and by microscopically evaluating the amount of regeneration and fibrosis. Results No significant difference was observed among the groups in terms of whisker movement and electrophysiological parameters (P > 0.05). We found that the numbers of regenerating myelinated fibers were significantly increased (P < 0.05) when BC tubes were used as a nerve conduit. Conclusions BC can be easily shaped into a hollow tube that guides nerve axons, resulting in better nerve regeneration after transection.Publication Metadata only Possible anti-inflammatory, antioxidant, and neuroprotective effects of apigenin in the setting of mild traumatic brain injury: an investigation*(2022-10-01) KOYUNCUOĞLU, TÜRKAN; YÜKSEL, MERAL; PEKER EYÜBOĞLU, İREM; AKAKIN, DİLEK; Kuru Bektasoglu P., Demir D., Koyuncuoglu T., YÜKSEL M., PEKER EYÜBOĞLU İ., Karagoz Koroglu A., AKAKIN D., Yildirim A., Celikoglu E., Gurer B.Objective Apigenin is a plant flavone proven with biological properties such as anti-inflammatory, antioxidant, and antimicrobial effects. This study, it was aimed to examine the possible anti-inflammatory, antioxidant, and neuroprotective effects of apigenin in the setting of the mild traumatic brain injury (TBI) model. Methods Wistar albino male rats were randomly assigned to groups: control (n = 9), TBI (n = 9), TBI + vehicle (n = 8), and TBI + apigenin (20 and 40 mg/kg, immediately after trauma; n = 6 and n = 7). TBI was performed by dropping a 300 g weight from a height of 1 m onto the skull under anesthesia. Neurological examination and tail suspension tests were applied before and 24 h after trauma, as well as Y-maze and object recognition tests, after that rats were decapitated. In brain tissue, luminol- and lucigenin-enhanced chemiluminescence levels and cytokine ELISA levels were measured. Histological damage was scored. Data were analyzed with one-way ANOVA. Results After TBI, luminol (p < .001) and lucigenin (p < .001) levels increased, and luminol and lucigenin levels decreased with apigenin treatments (p < .01-.001). The tail suspension test score increased with trauma (p < .01). According to the pre-traumatic values, the number of entrances to the arms (p < .01) in the Y-maze decreased after trauma (p < .01). In the object recognition test, discrimination (p < .05) and recognition indexes (p < .05) decreased with trauma. There was no significant difference among trauma apigenin groups in behavioral tests. Interleukin (IL)-10 levels, one of the anti-inflammatory cytokines, decreased with trauma (p < .05), and increased with 20 and 40 mg apigenin treatment (p < .001 and p < .01, respectively). The histological damage score in the cortex was decreased in the apigenin 20 mg treatment group significantly (p < .05), but the decrease observed in the apigenin 40 mg group was not significant. Conclusion The results of this study revealed that apigenin 20 and 40 mg treatment may have neuroprotective effects in mild TBI via decreasing the level of luminol and lucigenin and increasing the IL-10 levels. Additionally, apigenin 20 mg treatment ameliorated the trauma-induced cortical tissue damage.Publication Metadata only The effects of long-term prenatal exposure to 900, 1800, and 2100 MHz electromagnetic field radiation on myocardial tissue of rats(2022-11-01) AKAKIN, DİLEK; Bozok S., Karaagac E., Sener D., AKAKIN D., TÜMKAYA L.It is well-known that wireless communication technologies facilitate human life. However, the harmful effects of electromagnetic field (EMF) radiation on the human body should not be ignored. In the present study, we evaluated the effects of long-term, prenatal exposure to EMF radiation on the myocardium of rats at varying durations. Overall, 18 pregnant Sprague-Dawley rats were assigned into six groups (n = 3 in each group). In all groups other than the control group, three pregnant rats were exposed to EMF radiation (900, 1800 and 2100 MHz) for 6, 12 and 24 h over 20 days. After delivery, the newborn male pups were identified and six newborn male pups from each group were randomly selected. Then, histopathological and biochemical analysis of myocardial samples were performed. When 24-h/day prenatal exposures to 900, 1800, 2100 MHz EMF radiation were evaluated, myocardial damage was greater in the 2100 MHz EMF-24h group than the other groups. In addition, when malondialdehyde (MDA) and glutathione (GSH) levels associated with reactive oxidative species (ROS) were evaluated, the MDA level was higher in the 2100 MHz EMF-24h group compared with the other groups. The GSH level was also lower in the 2100 MHz EMF-24h group. When the 6, 12 and 24 h/day prenatal exposures to 1800 MHz EMF radiation were evaluated, myocardial damage was greater in 1800 MHz EMF-24h group than the remaining groups (p < 0.0001). Also, MDA level was greater in the 1800 MHz EMF-24h group compared with the other groups while the GSH level was lower in this group. It was shown that myocardial tissue was affected more by long-term exposure to EMF radiation at high frequencies. The data raise concerns that the harmful effects of non-ionizing radiation exposure on cardiac tissue will increase with 5G technology.Publication Metadata only Histopathological Evaluation of Mechanoreceptors in the Metatarsophalangeal Joint Capsule in Hallux Valgus(ELSEVIER SCIENCE INC, 2020) AKAKIN, DİLEK; Sirin, Evrim; Kandemir, Cansu; Yilmaz, Baris; Ozdemir, Guzelali; Akakin, Dilek; Muratli, Hasan HilmiTo date, we could find no study concerning the relationship between mechanoreceptors in the joint capsule of the first metatarsophalangeal joint and hallux valgus deformity. We aimed to investigate the presence of mechanoreceptors in samples obtained from the first metatarsophalangeal joint capsules of patients with hallux valgus deformity to improve our understanding of the clinical and histopathological features of the disease. Samples were taken from the first metatarsophalangeal joint capsules of 13 fresh-frozen cadavers with normal anatomy (controls) and 29 patients undergoing surgery for hallux valgus (cases). For light microscopy, excised specimens were fixed in 10% formaldehyde and processed for routine histopathological investigation. All samples were dehydrated in a series of ethanol, cleared in xylene, and embedded in paraffin. Orientation of collagen fibers was determined on Masson's trichrome-stained sections, and mechanoreceptors were evaluated on S-100-immunostained sections. In the sections stained with Masson's trichrome, the orientation of collagen fibers was regular in the control group. However, coarse and disoriented collagen bundles were observed in the hallux valgus cases (P <=.05). S-100 immunostaining was positive in the sections of both the cases and controls. Finally, free nerve endings were more abundant in the samples obtained from the capsules of hallux valgus cases than from the control group (P <= .05). An increase in the number of free nerve endings within the capsules of the first metatarsophalangeal joints in feet with hallux valgus deformity might have a role in the development of clinically relevant joint pain and instability. (C) 2020 by the American College of Foot and Ankle Surgeons. All rights reserved.Publication Metadata only Neuroprotective Effect of Plasminogen Activator Inhibitor-1 Antagonist in the Rat Model of Mild Traumatic Brain Injury(SPRINGER/PLENUM PUBLISHERS, 2021) ERZİK, CAN; Kuru Bektasoglu, Pinar; Koyuncuoglu, Turkan; Akbulut, Selin; Akakin, Dilek; Eyuboglu, Irem Peker; Erzik, Can; Yuksel, Meral; Kurtel, HizirPlasminogen activator inhibitor-1 (PAI-1) antagonists are known for their neuroprotective effects. In this study, it was aimed to investigate the possible protective effects of PAI-1 antagonists in a rat mild traumatic brain injury (TBI) model. Sprague-Dawley male rats were grouped as sham (n = 7), TBI (n = 9), and TBI + PAI-1 antagonist (5 and 10 mg/kg TM5441 and TM5484; n = 6-7). Under anesthesia, TBI was induced by dropping a metal 300-g weight from a height of 1 m on the skull. Before and 24-h after trauma neurological examination, tail suspension, Y-maze, and novel object recognition tests were performed. Twenty-four hours after TBI, the rats were decapitated and activities of myeloperoxidase, nitric oxide release, luminol-, and lucigenin-enhanced chemiluminescence were measured. Also, interleukin-1 beta, interleukin-6, tumor necrosis factor, interleukin-10, tumor growth factor-beta, caspase-3, cleaved caspase-3, and PAI levels were measured with the ELISA method in the brain tissue. Brain injury was graded histopathologically following hematoxylin-eosin staining. Western blot and immunohistochemical investigation for low-density lipoprotein receptor, matrix metalloproteinase-3, and nuclear factor-kappa B were also performed. Data were analyzed using GraphPad Prism 8.0 (GraphPad Software, San Diego, CA, USA) and expressed as means +/- SEM. Values of p < 0.05 were considered to be statistically significant. Higher levels of myeloperoxidase activity in the TBI group (p < 0.05) were found to be suppressed in 5 and 10 mg/kg TM5441 treatment groups (p < 0.05-p < 0.01). The tail suspension test score was increased in the TBI group (p < 0.001) and decreased in all treatment groups (p < 0.05-0.001). The histologic damage score was increased statistically significantly in the cortex, dentate gyrus, and CA3 regions in the TBI group (p < 0.01-0.001), decreased in the treatment groups in the cortex and dentate gyrus (p < 0.05-0.001). PAI antagonists, especially TM5441, have antioxidant and anti-inflammatory properties against mild TBI in the acute period. Behavioral test results were also improved after PAI antagonist treatment after mild TBI.Publication Metadata only 3-boyutlu biyoyazıcı teknolojisi ile kök hücre yüklü nanohibrit kemik/kıkırdak doku iskelelerinin geliştirilmesi: in vitro ve in vivo değerlendirilmesi(2022-09-06) ALARÇİN, EMİNE; AKAKIN, DİLEK; BAL ÖZTÜRK A., İzbudak B., ALARÇİN E., Yüce E., Kaya K., KOZACI L. D. , KARAGÖZ KÖROĞLU A., Kocaağa B., GÜNER F. S. , AKAKIN D., et al.Osteokondral doku hasarı, hastalıktan travmaya kadar farklı nedenlerle ortaya çıkabilen ciddi bir sağlık sorunudur. Osteokondral arayüz defektleri yaygın olarak hem kıkırdak (hiyalin) hem de destekleyici subkondral kemiğin lezyonlarını içermektedir. Hasarın ortopedik cerrahi ile fonksiyonel onarımı, osteokondral yapının karmaşıklığı göz önüne alındığında oldukça zordur. Osteokondral doku mühendisliği, kemik, kıkırdak ve kemik-kıkırdak arayüzünün onarımı ve yenilenmesi için; yapısal iskelelerin, biyoaktif moleküllerin ve hücrelerin kullanımı ile bu zorlukların üstesinden gelmeyi amaçlamaktadır. Gözenekli iskeleler, lifler ve hidrojeller gibi çeşitli matris yapıları, aynı anda kıkırdak, orta kalsifiye kıkırdak ve kemik dokularını restore etmek için tasarlanmaktadır (Yang ve ark., 2017; Ai ve ark., 2021; Wei ve ark., 2021).Bu çalışmada, 3B-biyoyazıcı tekniği ile kök hücre yüklü, TGF-ß1 ve rhBMP-2 salımı yapabilen çift-tabakalı nanohibrit hidrojel temelli doku iskeleleri geliştirilmiştir. Doku iskeleleri 3B-biyobaskı yöntemi ile jelatin metakrilat (GelMA), kitosan metakrilat (KitMA) ve hidrotermal metot ile üretilen LDH nanopartiküllerinin (çift katmanlı hidroksit bileşikleri) Irgacure2959 fotobaşlatıcı varlığında yazdırılması ve sonrasında UV ışık altında kürlenmesi ile hazırlanmıştır. LDH nanopartiküllerine yüklenen TGF-ß1 ve rhBMP-2 sinyal molekülerinin nanopartiküllerden ve doku iskelelerinden in vitro salım davranışları enzim-bağlı immünosorbent testi (ELISA) ile incelenmiştir. Fiziksel, kimyasal ve mekaniksel özellikleri karakterize edilen doku iskelelerine enkapsüle edilen kök hücrelerin canlılığı (canlı/ölü testi), morfolojisi (DAPI/Aktin boyama), gelişimi, proliferasyonu (PrestoBlue analizi), osteojenik ve kondrojenik farklılaşma özellikleri (immünfloresan boyamalar ve PCR analizleri) incelenmiştir. Doku iskelelerinin kemik ve kıkırdak onarımına etkisi sıçanlar üzerinde in vivo osteokondral hasar modelinde değerlendirilmiştir. Tüm sonuçlar birlikte değerlendirildiğinde, geliştirilen nanohibrit çift-tabakalı platformun osteojenik/kondrojenik farklılaşmayı desteklediği ve osteokondral rejenerasyonu teşvik ettiği sonucuna varılmıştırPublication Metadata only Effect of Bumetanide on Facial Nerve Regeneration in Rat Model(SAGE PUBLICATIONS LTD, 2021) AKAKIN, DİLEK; Longur, Ecem Sevim; Yigit, Ozgur; Ertugay, Cigdem Kalaycik; Server, Ela Araz; Adatepe, Turgut; Akakin, Dilek; Orun, Oya; Koroglu, Ayca KaragozObjective We investigated the effects of bumetanide alone and in combination with dexamethasone on facial nerve regeneration in rats with facial paralysis. Study Design A prospective controlled animal study. Setting An animal laboratory. Subjects and Methods Facial paralysis was induced in 32 Wistar rats that we then divided into 4 groups: group 1, control; group 2, bumetanide; group 3, dexamethasone; group 4, bumetanide and dexamethasone. Electroneurography was performed 1, 2, and 4 weeks later, and nerve regeneration was evaluated by electron and light microscopy and Western blotting in week 4. Results Regarding the comparison between preoperative values and week 4, the latency difference in group 1 (1.25 milliseconds) was significantly higher than those of groups 2 to 4 (0.56, 0.34, and 0.10 milliseconds, respectively;P= .001). The latency increment in groups 2 and 3 was higher than that of group 4 (P= .002 andP= .046) in week 4, whereas groups 2 and 3 did not differ significantly (P= .291). Amplitude difference was not statistically significant from week 4 among all groups (allP >.05). The number of myelinated axons was significantly higher in all treatment groups than in the control group (P= .001). Axon number and intensity were significantly higher in group 4 as compared with groups 2 and 3 (P= .009,P= .005). Conclusion After primary neurorrhaphy, dexamethasone and bumetanide alone promoted nerve recovery based on electrophysiologic and histologic measures. Combination therapy was, however, superior.Publication Metadata only Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats(TAYLOR & FRANCIS INC, 2020) AKAKIN, DİLEK; Sirvanci, Serap; Akakin, Dilek; Idrizoglu, Medine Gulcebi; Kaya, Ozlem Tugce; Karamahmutoglu, Tugba; Asik, Zehra Nur Turgan; Onat, FilizGenetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata anterior (SNRa) and posterior (SNRp) regions between GAERS and Wistar rats receiving kindling stimulations. Animals in the kindling group either received six stimulations in the amygdala and had grade 2 seizures or they were kindled, having grade five seizures. Rats were decapitated one hour after the last stimulation. SNR regions were obtained after vibratome sectioning of the brain tissue. GABA immunoreactivity was detected by immunogold method and synapses were counted. Sections were observed by transmission electron microscope and analyzed by Image J program. GABA density in the SNRa region of fully kindled GAERS and Wistar groups increased significantly compared to that of their corresponding grade 2 groups. The number of synapses increased significantly in kindled and grade 2 GAERS groups, compared to kindled and grade 2 Wistar groups, respectively, in the SNRa region. GABA density in the SNRp region of kindled GAERS group increased significantly compared to that of GAERS grade 2 group. In the SNRp region, both kindled and grade 2 GAERS groups were found to have increased number of synapses compared to that of GAERS control group. We concluded that both SNRa and SNRp regions may be important in modulating resistance of GAERS to kindling stimulations.Publication Metadata only High intensity interval training protects from Post Traumatic Stress Disorder induced cognitive impairment(ELSEVIER, 2021) AKAKIN, DİLEK; Koyuncuoglu, Turkan; Sevim, Hacer; Cetrez, Nursen; Meral, Zeynep; Gonenc, Berfin; Dertsiz, Ekin Kuntsal; Akakin, Dilek; Yuksel, Meral; Cakir, Ozgur KasimayThis study aimed to show the possible protective effects of high intensity interval training (HIIT) in PTSD-induced rats and probable underlying mechanisms. Female rats (n = 44) were separated as; Sedentary (SED), moderate intensity continuous training (MICT), HIIT groups. Then the groups were divided into subgroups according to PTSD induction (n = 6-8/group). Exercise groups performed HIIT or MICT for 6 weeks. On the fifth week, PTSD was induced by single prolonged stress protocol. Cognitive functions were evaluated by object recognition, anxiety levels by hole-board and elevated plus maze, and fear conditioning by passive avoidance tests. Following decapitation, malondialdehyde (MDA), glutathione (GSH), luminol and lucigenin chemiluminescence levels, and myeloperoxidase (MPO), superoxide dismutase (SOD) and catalase (CAT) activities were measured, and histopathological damage was evaluated. The data was analyzed by one-way ANOVA. Cognitive decline and aggravated anxiety levels in SED + PTSD group were improved in both PTSD-induced exercise groups (p < 0.05-0.001). The increased chemiluminescence levels, MPO activity and histological damage were depressed in both PTSD-induced exercise groups (p < 0.05-0.001). The risen MDA levels in SED + PTSD group were suppressed only in HIIT + PTSD group (p < 0.01-0.001). The decreased GSH levels were increased by MICT (p < 0.05-0.001), and CAT and SOD activities were improved via HIIT < 0.05). Compared to SED group, latency was decreased in SED + PTSD < 0.05-0.01) group. Neuronal damage scores were alleviated in both PTSD-induced exercise groups (p < 0.001). PTSD-induced memory decline was protected by both of the exercise models however more effectively by HIIT via decreasing oxidative stress, anxiety levels and by improving antioxidant capacity as a protective system for neuronal damage.