Person: ÇETİNEL, ŞULE
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ÇETİNEL
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ŞULE
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Publication Metadata only Taurine ameliorates stress-induced degeneration of the urinary bladder(ELSEVIER GMBH, URBAN & FISCHER VERLAG, 2007) ERCAN, FERİHA; Zeybek, Ali; Saglam, Beyhan; Cikler, Esra; Cetinel, Sule; Ercan, Feriha; Sener, GokselWe studied the potential effects of taurine, a free radical scavenger, on chronic water avoidance stress (WAS)-induced degeneration of the mucosa of the urinary bladder in experimental rats. Wistar albino rats were exposed to WAS for 2 h/day, for 5 days (WAS group). Before exposing them to WAS, taurine (50 mg/kg) (WAS+taurine group) was injected intraperitonally into the animals. Samples of urinary bladder were then investigated by light and scanning electron microscopy. Lipid peroxidation and gluthathione levels were also measured in the urinary bladder. In the WAS-only group, inflammatory cell infiltration, increased number of mast cells in the mucosa and ulcerated areas were observed. In the WAS+taurine group, relatively normal urothelial topography with microvilli, moderate inflammatory cell infiltration and decreased numbers of mast cells in the mucosa were observed. The increased lipid peroxidation and decreased glutathione levels in WAS rats were reversed by taurine treatment. We conclude that taurine protects against WAS-induced oxidant urinary bladder injury, and thus may be a possible therapeutic agent against interstitial cystitis, the symptoms of which are aggravated by stress conditions. (c) 2006 Elsevier GmbH. All rights reserved.Publication Metadata only Protective effects of taurine on protamine sulfate induced bladder damage(SPRINGER, 2006) ERCAN, FERİHA; Zeybek, Ali; Saglam, Beyhan; Cikler, Esra; Cetinel, Sule; Ercan, Feriha; Sener, GokselThe present study was designed to investigate the putative protective effects of taurine on protamine sulfate (PS) induced bladder injury. Wistar albino female rats were catheterized and intravesically infused with phosphate buffered solution (control group) or PS (PS group) dissolved in phosphate buffered solution. In the PS + taurine (PS+Tau) group, after the PS instillation, taurine (50 mg/kg) was injected intraperitoneally for 3 days. Histopathological changes were investigated by light and scanning electron microscopy. Tissue samples were also obtained to determine bladder malondialdehyde (MDA) (a biomarker of oxidative damage) and glutathione (GSH) (a biomarker of protective oxidative injury) levels. In the PS group ulcerated areas, an irregular mucus layer, inflammatory cell infiltration, and increased number of mast cells were observed. In the PS+Tau group, a relatively normal urothelial topography, glycosaminoglycan layer, and decreased number of mucosal mast cells and inflammatory cells were observed. Increased MDA levels as a result of PS induction lead us to propose that free radicals may have a critical role in this injury. The significant decrease in MDA and increase in GSH levels in the PS+Tau group compared to PS group was in accordance with morphological findings. Based on the results, taurine treatment significantly prevented PS induced degenerative morphological and biochemical changes of urinary bladder mucosa.Publication Metadata only Leukotriene D4 receptor antagonist montelukast alleviates water avoidance stress-induced degeneration of the gastrointestinal mucosa(ELSEVIER SCI LTD, 2008) ERCAN, FERİHA; Ersoy, Yasemin; Cikler, Esra; Cetinel, Sule; Sener, Goekel; Ercan, FerihaWe investigated the role of montelukast (ML), a cysteinyl leukotriene-1 receptor antagonist, on the water avoidance stress (WAS)induced degeneration of the rat gastric, ileal and colonic mucosa. One group of Wistar albino rats were exposed to chronic WAS (WAS group) 2 h daily for 5 days. Another group was administered ML (10 mg/kg; i.p.; WAS + ML group) following every WAS exposure for 5 days. Control rats were injected with the vehicle solution only. The stomach, ileum and colon were dissected and investigated for histopathological changes with a light microscope as well as for topographical changes with a scanning electron microscope. The levels of malondialdehyde (MDA, a biomarker of oxidative damage) and glutathione (GSH, a biomarker of protective oxidative injury) were also determined in all dissected tissues. In the WAS group, the stomach epithelium showed ulceration in some areas, dilatations of the gastric glands, degeneration of gastric glandular cells, and prominent congestion of the capillaries. In a similar fashion, degenerated epithelium and severe vascular congestions were observed in the ileum and colon. In all the tissues dense inflammatory cell infiltration and mast cell degranulation in mucosa were observed. The levels of MDA were significantly increased whereas those of GSH were significantly decreased in all test tissues in the WAS group compared to the control group. The morphology of gastric, ileal and colonic mucosa in WAS + ML group showed a significant amelioration showing a reduction in inflammatory cell infiltration and mast cell degranulation. Increased NIDA and decreased GSH levels in the WAS group were also ameliorated with ML treatment. Based on the results, ML supplement seems attenuated inflammatory effects of WAS induction in gastrointestinal mucosa. (C) 2008 Elsevier Ltd. All rights reserved.Publication Metadata only Protective Effects of Montelukast Against Stress-Induced Degeneration of the Urinary Bladder(MARMARA UNIV, INST HEALTH SCIENCES, 2018) ERCAN, FERİHA; Dulger, Esra Cikler; Canillioglu, Yasemin Ersoy; Cetinel, Sule; Sener, Goksel; Ercan, FerihaObjective: The aim of the study was to investigate the role of montelukast (ML), a cysteinyl leukotriene-1 receptor antagonist, on the water avoidance stress (WAS) - induced degeneration of the rat urinary bladder mucosa. Methods: In WAS group, rats were exposed to WAS two hours daily for five days. In WAS+ML group, rats were administered ML (10 mg/kg; i.p.;) following every WAS exposure for 5 days. In control group, rats were injected vehicle solution only. The urinary bladder was evaluated for general morphology at light microscope. Mast cell activation and uroplakin distribution were assessed with immunohistochemistry. Glycosaminoglycan (GAG) distribution and urothelial permeability were observed using ruthenium red (RR) staining techniques in transmission electron microscope and luminal urothelial cells were observed with scanning electron microscope. Tissue malondialdehyde (MDA) and gluthatione (GSH) levels were also analysed. Results: Irregular GAG layer and uroplakin distribution, penetration of RR in the intercellular spaces and dilated tight junctions in urothelial layer, increase in inflammatory cell infiltration, in number of both granulated and activated mast cells, and were observed in the WAS group. The MDA level was increased, and GSH level was decreased significantly in urinary bladder in the WAS group in comparison with the control group. Quite regular GAG layer, uroplakin distribution and tight junctions in most regions, decrease in inflammatory cell infiltration and both of activated and granulated mast cells in the mucosa, were observed in WAS+ML group. Moreover, significant decrease in MDA and increase in GSH levels were observed in this group. Conclusion: Montelukast appears to exert a protective activity in WAS induced urinary bladder injury by inhibiting inflammatory and oxidative activity.