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ÖZBAŞ, SUNA

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2015 - 20172
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Author: ŞEN, ALİ × Has files: true ×

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    PublicationOpen Access
    In vitro antiproliferative activity of Endemic Centaurea kilaea Boiss. against Human Tumor Cell Lines
    (AVES PRESS LTD, 2015) ŞEN, ALİ; Sen, Ali; Ozbas, Suna Turan; Akbuga, Julide; Bitis, Leyla
    Objective: In this study, it is aimed to determine the active extract on which substance isolation through bioactivity-guided method is to be performed in the future. Therefore, it is planned to reveal the extract exhibiting the highest antiproliferative activity by investigating the activities of heptane (CKH), chloroform (CKC), sub-fractions of active chloroform (CKCSI, CKCSII, CKCSIII) and methanol extracts (CKM) obtained from the aerial parts of Centaurea kilaea Boiss. (Asteraceae) on different cancerous cell lines. Method: Antiproliferative activity was measured against three human cancer cell lines (Hela; cervix adenocarcinoma, MCF-7; breast adenocarcinoma, PC-3; prostate adenocarcinoma) using MTT assay. Results: CKC exhibited the greatest antiproliferative activity with IC50 of 53.07; 68.64 mu g/ml against Hela and MCF-7 cells, respectively, while CKC and CKM showed the highest antitumor activity against PC-3 cell (73.92; 70.11 mu g/ml, respectively). Also, a sub-fraction II of active chloroform extract (CKCSII) demonstrated moderate antiproliferative activity with IC50 values of 60.75 and 60.70 mu g/ml against Hela and MCF-7 cells, respectively. Conclusion: The results show that CKC and CKCSII are good candidates for further bioactivity-guided fractionation in the search for new active antitumor compounds. Also, these findings confirm other results that have been reported in the literature relating to antiproliferative activities of different Centaurea species. Furthermore, active extracts would be tested on different tumor cell lines.
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    PublicationOpen Access
    Bioactivity-guided isolation of anti-proliferative compounds from endemic Centaurea kilaea
    (TAYLOR & FRANCIS LTD, 2017-01-01) ŞEN, ALİ; Sen, Ali; Turan, Suna Ozbas; Bitis, Leyla
    Context: The genus Centaurea L. (Asteraceae) is one of the largest genera in Turkey. Compounds and extracts obtained from different Centaurea species have significant anti-cancer activity against various cancer cell lines. Objective: To determine the anti-proliferative activity of isolates from the chloroform extract of C. kilaea Boiss. Materials and methods: Eleven compounds were isolated using column chromatography and preparative TLC from the chloroform extract of aerial parts of endemic C. kilaea. The structures of the isolated compounds were elucidated by various spectroscopic methods, including UV, H-1-NMR and C-13-NMR. Anti-proliferative activity of compounds (0.5-50 mu g/mL) were measured against one normal cell line (L-929, mouse fibroblast) and three human cancer cell lines (Hela, cervix carcinoma; MCF-7, breast carcinoma; PC-3, prostate carcinoma) using MTT assay. Results were expressed as IC50 values. Results: None of the 11 compounds displayed activity against L-929 and HeLa. Two of these compounds, cnicin and cirsimaritin, showed fairly strong activity against MCF-7 and PC-3 with IC50 values of 3.25 and 4.3 mu g/mL, respectively. Discussion and conclusion: This is the first report on cirsimaritin. Cirsimaritin and cnicin could serve as potential anti-cancer drug candidates against breast and prostate cancer, respectively.