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KAYA, ÖZLEM TUĞÇE

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KAYA

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ÖZLEM TUĞÇE

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2011 - 20172
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End date: 2019 × Subject: DENTATE GYRUS ×

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    Demonstration of Doublecortin Protein, a Neurogenesis Marker, at the Electron Microscopic Level
    (AVES PRESS LTD, 2017) KAYA, ÖZLEM TUĞÇE; Kaya, Ozlem Tugce Cilingir; Moore, Cynthia; Meshul, Charles; Sirvanci, Serap
    Objective: Demonstration of newly born neurons in adult brains is an important issue in terms of elucidating neurogenesis. In our study, we aimed to develop an appropriate pre-embedding microwave labeling method for the newly born neuronal marker doublecortin (DCX) protein in the hippocampal dentate gyrus (DG) region. Methods: Brains were obtained from 10-week-old C57BJ/6J male mice by perfusion fixation. Vibratome sections from brain tissues were labeled with an anti-DCX antibody using a pre-embedding microwave method. Sections stained with 3.3'-diaminobenzidine (DAB) were prepared for electron microscopic (EM) analyses using a microwave. After embedding in Epon, thin sections were obtained, observed under an electron microscope, and photographed for morphological assessments. Results: DCX labeling performed using the pre-embedding microwave method was specifically demonstrated at both light and electron microscopic levels. DCX-positive cells were localized at the subgranular zone and granular layer. Electron microscopic observations showed that DCX immunoreactivity was positive at the axons, dendrites, and somata. Conclusion: We demonstrated that the existence of DCX can be determined using the pre-embedding microwave labeling as an immunoelectron method in the DG region. Our study provides a basis for further studies on neurogenesis aiming to show DCX-immunoreactive cells using the pre-embedding DAB labeling method at the electron microscopic level.
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    Ultrastructural GABA immunocytochemistry in the mossy fiber terminals of Wistar and genetic absence epileptic rats receiving amygdaloid kindling stimulations
    (ELSEVIER, 2011) AKAKIN, DİLEK; Akakin, Dilek; Sirvanci, Serap; Gurbanova, Ayten; Aker, Rezzan; Onat, Filiz; San, Tangul
    The existence of absence epilepsy and temporal lobe epilepsy in the same patient is not common in clinical practice. The reason why both types of seizures are rarely seen in the same patient is not well understood. Therefore, we aimed to investigate kindling in a well known model of human absence epilepsy, genetic absence epilepsy rats from Strasbourg (GAERS). In the present study, we analyzed whether the GABA content of GAERS that received kindling stimulations was altered in the hippocampal mossy fiber terminals compared to non-epileptic control (NEC) Wistar rats. For this purpose, we used an immunocytochemical technique at the ultrastructural level. Ultrathin sections were immunolabeled with anti-GABA antibody and transmission electron microscopy was used for the ultrastructural examination. The number of gold particles per nerve terminal was counted and the area of the nerve terminal was determined using NIH image analysis program. The GABA density was found to be higher in sham-operated GAERS than sham-operated Wistar rats. The density was increased in kindling Wistar group compared to sham-operated Wistar and kindling GAERS groups. No statistical difference was observed between sham-operated GAERS and kindling GAERS groups. The increase in GABA levels in stimulated Wistar rats may be a result of a protective mechanism. Furthermore, there may be strain differences between Wistar rats and GAERS and our findings addressing different epileptogenesis mechanisms in these strains might be a basis for future experimental studies. (C) 2010 Elsevier B.V. All rights reserved.