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ŞAHİN, ALİ

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ŞAHİN

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Author: AKSU, MEHMET BURAK ×

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    PublicationOpen Access
    Fabrication of gentamicin sulfate-loaded 3d-printed polyvinyl alcohol/sodium alginate/gelatin-methacryloyl hybrid scaffolds for skin tissue replacement
    (2023-01-01) ULAĞ, SONGÜL; ŞAHİN, ALİ; AKSU, MEHMET BURAK; GÜNDÜZ, OĞUZHAN; Izgordu M. S., Ayran M., ULAĞ S., Yildirim R., Bulut B., ŞAHİN A., Guncu M. M., AKSU M. B., GÜNDÜZ O.
    3D-printed scaffolds can better mimic the function of human skin, both biologically and mechanically. Within the scope of this study, the effect of the addition of different amounts (10, 15, 20 mg) of gentamicin sulfate (GS) to a 10 mL solution of natural and synthetic polymers is investigated. Sodium alginate (SA), gelatin-methacryloyl (GelMA), and polyvinyl alcohol (PVA) are chosen as bioactive materials. The surface morphology and pore structures are visualized by scanning electron microscopy (SEM). According to the results, it is observed that the pore sizes of all scaffolds are smaller than 270 µm, the lowest value (130 µm) is obtained in the scaffold loaded with 15 mg GS, and it also has the highest tensile strength value (12.5 ± 7.6 MPa). Similarly, it is observed that the tensile strength (9.7 ± 4.5 MPa) is high in scaffold loaded with 20 mg GS. The biocompatibility test is performed with fibroblast cells, and the results show that the scaffolds are biocompatible with cells. The antibacterial test is carried out against the S.aureous and E. coli and the results indicate that all GS-loaded scaffolds demonstrate antibacterial activity.
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    PublicationOpen Access
    Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis
    (MDPI, 2021-04-28) ŞAHİN, ALİ; Ulag, Songul; Ilhan, Elif; Demirhan, Ramazan; Sahin, Ali; Yilmaz, Betul Karademir; Aksu, Burak; Sengor, Mustafa; Ficai, Denisa; Titu, Aurel Mihail; Ficai, Anton; Gunduz, Oguzhan
    In this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa, and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus, but for P. aeruginosa, more studies should be performed.
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    A novel approach to treat the Thiel-Behnke corneal dystrophy using 3D printed honeycomb-shaped polymethylmethacrylate (PMMA)/Vancomycin (VAN) scaffolds
    (Elsevier B.V., 2021) ŞAHİN, ALİ; Ulag S., Sahin A., Guncu M.M., Aksu B., Ekren N., Sengor M., Kalaskar D.M., Gunduz O.
    Thiel-Behnke corneal dystrophy, or honeycomb corneal dystrophy, is an autosomal dominant corneal disorder. Tissue engineering can be a novel approach to regenerate this dystrophy. In this study, the honeycomb geometry of the dystrophy mimicked with a 3D printing technology, and 40% PMMA, 40% PMMA/(0.1, 0.5, 2, and 10)% VAN scaffolds were fabricated with honeycomb geometry. As a result of the biocompatibility test with mesenchymal stem cells (MSCs), it can be said that cells on the scaffolds showed high viability and proliferation for all incubation periods. According to the antibacterial activity results, the 40% PMMA/10% VAN showed antibacterial activity against S. aureous. Mechanical results reported that with the addition of VAN into the 40% PMMA, the tensile strength value increased up to 2% VAN amount. The swelling behaviours of the scaffolds were examined in vitro, and found that the swelling rate increased with a high VAN amount. The release of VAN from the scaffolds showed sustained release behaviour, and it took 13 days to be released entirely from the scaffolds. © 2021 Elsevier B.V.