Person:
ŞAHİN, ALİ

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

ŞAHİN

First Name

ALİ

Name

Filters

2015 - 201952020 - 202316
Reset filters

Settings

End date: 2024 × Has files: false ×

Search Results

Now showing 1 - 10 of 21
  • No Thumbnail Available
    PublicationMetadata only
    Antitumor and antimetastatic effects of walnut oil in esophageal adenocarcinoma cells
    (CHURCHILL LIVINGSTONE, 2018) ŞAHİN, ALİ; Batirel, Saime; Yilmaz, Ayse Mine; Sahin, Ali; Perakakis, Nikolaos; Ozer, Nesrin Kartal; Mantzoros, Christos S.
    Background: Walnuts contain many components including specific fatty acids, which could be active against cancer. Even though the anticarcinogenic effect of some of the individual fatty acids in walnut oil has been described, the effect of walnut oil itself on esophageal cancer cells hasn't yet been investigated. Objective: We aimed to investigate whether walnut oil affects tumor growth and metastatic potential in esophageal cancer cells. Methods: The human esophageal adenocarcinoma cell line, OE19, was treated with different doses of walnut oil and cell viability, apoptosis/necrosis and cell cycle analyses were performed using WST-1 assay and flow cytometry respectively. Adhesion, colony formation and wound healing assays were performed to assess the antimetastatic effects of walnut oil. NFkB expression was evaluated with western blot analysis. Results: Walnut oil decreased the cell viability of esophageal cancer cells in a dose-dependent manner. 20 mg/mL walnut oil reduced cell viability by similar to 50% when compared with control. The analysis revealed that necrosis and accumulation of cells in G0/G1 phase was induced in the cells treated with high doses of walnut oil. It also down-regulated the protein levels of NFkB. Walnut oil suppressed the adhesion, migration and colony formation of the cells. Conclusions: High-dose short-term administration of walnut oil reduces the cell viability and metastatic ability of esophageal cancer cells, while exhibiting anticarcinogenic effect by inducing necrosis and cell cycle arrest at the G0/G1 phase, probably through suppression of the NFkB pathway. These data indicate that walnut oil, and by extension walnut consumption, may have beneficial effects in esophageal cancer in humans. This should be tested by clinical trials in the future. (C) 2017 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
  • No Thumbnail Available
    PublicationMetadata only
    Evidence that osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived stem cells was more pronounced than in subcutaneous cells
    (TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2020) ŞAHİN, ALİ; Solmaz, Bilgehan; Sahin, Ali; Kelestemur, Taha; Kilic, Ertugrul; Kaptanoglu, Erkan
    Background/aim: The management of dura-related complications, such as the repairment of dural tears and reconstruction of large dural defects, remain the most challenging subjects of neurosurgery. Numerous surgical techniques and synthetic or autologous adjuvant materials have emerged as an adjunct to primary dural closure, which may result in further complications or side effects. Therefore, the subcutaneous autologous free adipose tissue graft has been recommended for the protection of the central nervous system and repairment of the meninges. In addition, human adipose tissue is also a source of multipotent stem cells. However, epidural adipose tissue seems more promising than subcutaneous because of the close location and intercellular communication with the spinal cord. Herein, it was aimed to define differentiation capability of both subcutaneous and epidural adipose tissue-derived stem cells (ASCs). Materials and methods: Human subcutaneous and epidural adipose tissue specimens were harvested from the primary incisional site and the lumbar epidural space during lumbar spinal surgery, and ASCs were isolated. Results: The results indicated that both types of ASCs expressed the cell surface markers, which are commonly expressed stem cells; however, epidural ASCs showed lower expression of CD90 than the subcutaneous ASCs. Moreover, it was demonstrated that the osteogenic and neurogenic differentiation capability of epidural adipose tissue-derived ASCs was more pronounced than that of the subcutaneous ASCs. Conclusion: Consequently, the impact of characterization of epidural ASCs will allow for a new understanding for dural as well as central nervous system healing and recovery after an injury.
  • No Thumbnail Available
    PublicationMetadata only
    ER stress related lipid accumulation and apoptotic cell death in nonalcoholic fatty liver diesease
    (ELSEVIER SCIENCE INC, 2017) ŞAHİN, ALİ; Demirel, Tugce; Sozen, Erdi; Sahin, Ali; Karademir, Betul; Ozer, Nesrin Kartal
  • No Thumbnail Available
    PublicationMetadata only
  • No Thumbnail Available
    PublicationMetadata only
    The effect of polycaprolactone/graphene oxide electrospun scaffolds on the neurogenic behavior of adipose stem cells
    (Elsevier Ltd, 2022) ŞAHİN, ALİ; Pinar E., Sahin A., Unal S., Gunduz O., Harman F., Kaptanoglu E.
    Stem cell destiny can be controlled with scaffold biomaterials in tissue engineering and regenerative medicine. This study aimed to investigate the neuronal differentiation potential of human adipose tissue-derived mesenchymal stem cells in graphene nanofiber matrix in vitro. Stem cell isolation was performed from adipose tissue taken from human by mechanical and enzymatic methods. The differentiation potential was examined after incubation of adipose stem cells in normal medium and neural differentiation medium, on graphene oxide (GO) and polycaprolactone (PCL) composite scaffolds produced by electrospinning technique. In vitro studies indicated that the presence of GO in PCL scaffold increases an effect on cell attachment, proliferation, infiltration into the scaffold, and neuronal differentiation. Also, unlike subcutaneous tissue, it has been shown immunohistochemically that mesenchymal stem cells derived from epidural adipose tissue tend to differentiate into oligodendrocytes. © 2022 Elsevier Ltd
  • No Thumbnail Available
    PublicationMetadata only
    Design and fabrication of electrospun polycaprolactone/chitosan scaffolds for ligament regeneration
    (PERGAMON-ELSEVIER SCIENCE LTD, 2021) İNAN, AHMET TALAT; Saatcioglu, Elif; Ulag, Songul; Sahin, Ali; Yilmaz, Betul Karademir; Ekren, Nazmi; Inan, Ahmet Talat; Palaci, Yuksel; Ustundag, Cem Bulent; Gunduz, Oguzhan
    Tendon and ligament impairments are among the most familiar injuries of the knee with acute or chronic pain conditions. The defects of anterior cruciate ligament (ACL) stay a known clinical problem. In the present study, the electrospinning method was used to fabricate 10wt.%PCL/(1, 3, 5)wt.%Chitosan (CS) appropriate and biocompatible scaffolds with a similar connective ligament geometry and structure. 10wt.%PCL/3wt.%CS demonstrated higher tensile strength value (0.58854 MPa) than other scaffolds in the tensile test. Moreover, 10wt.%PCL/3wt.%CS scaffolds had high mesenchymal stem cells (MSCs) viability value for all incubation periods. Swelling and degradation behaviours of the ligament-like scaffolds were examined in vitro for 15 days. Results reported that the highest swelling ratio was observed with CS addition for 10wt.%PCL/5wt.%CS scaffolds which value nearly reached to the 270% ratio. Scanning electron microscope proved the geometry of the scaffolds, which were suitable for ligament-like tissue. Attachment of MSCs on the scaffolds proved the network-like structure of the cells on the scaffolds.
  • No Thumbnail Available
    PublicationMetadata only
    In vitro evaluation of biomaterials for neural tissue engineering
    (Elsevier Science, Oxford/Amsterdam , 2023-04-01) ŞAHİN, ALİ; YILMAZ, BETÜL; Şahin A., Çıkı B., Yılmaz B.
  • No Thumbnail Available
    PublicationMetadata only
    Production of 3D-Printed Tympanic Membrane Scaffolds as a Tissue Engineering Application
    (Springer, 2020) ŞAHİN, ALİ; Ilhan E., Ulag S., Sahin A., Ekren N., Kilic O., Oktar F.N., Gunduz O.
    In recent years, scaffolds produced in 3D printing technology have become more widespread tool due to providing more advantages than traditional methods in tissue engineering applications. In this research, it was aimed to produce patches for the treatment of tympanic membrane perforations which caused significant hearing loss by using 3D printing method. Polylactic acid (PLA) scaffolds with Chitosan (CS) added in various ratios were prepared for artificial eardrum patches. Different amounts of CS added to PLA to obtain more biocompatible scaffolds. The created patches were designed by mimicking the thickness of the natural tympanic membrane thanks to the precision provided by the 3D printed method. The produced scaffolds were analyzed separately for physical, chemical, morphological, mechanical and biocompatibility properties. Human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) were used for cell culture study to analyze the biocompatibility properties. 15 wt% PLA was chosen as the control group. Scaffold containing 3 wt% CS demonstrated significantly superior and favorable features in printing quality. The study continued with these two scaffolds (15PLA and 15PLA/3CS). This study showed that PLA and PLA/CS 3D printed scaffolds are a potential application for repairing tympanic membrane perforation. © Springer Nature Switzerland AG 2020.
  • No Thumbnail Available
    PublicationMetadata only
    Fabrication of tissue-engineered tympanic membrane patches using 3D-Printing technology
    (ELSEVIER, 2021) ŞAHİN, ALİ; Ilhan, Elif; Ulag, Songul; Sahin, Ali; Yilmaz, Betul Karademir; Ekren, Nazmi; Kilic, Osman; Sengor, Mustafa; Kalaskar, Deepak M.; Oktar, Faik Nuzhet; Gunduz, Oguzhan
    In recent years, scaffolds produced in 3D printing technology have become more widespread tool due to providing more advantages than traditional methods in tissue engineering applications. In this research, it was aimed to produce patches for the treatment of tympanic membrane perforations which caused significant hearing loss by using 3D printing method. Polylactic acid(PLA) scaffolds with Chitosan(CS) and Sodium Alginate(SA) added in various ratios were prepared for artificial eardrum patches. Different amounts of chitosan and sodium alginate added to PLA increased the biocompatibility of the produced scaffolds. The created patches were designed by mimicking the thickness of the natural tympanic membrane thanks to the precision provided by the 3D printed method. The produced scaffolds were analyzed separately for chemical, morphological, mechanical and biocompatibility properties. Scanning electron microscope (SEM), Fourier-transform infrared (FT-IR) spectroscopy was performed to observe the surface morphology and chemical structure of the scaffolds. Mechanical, thermal and physical properties, swelling and degradation behaviors were examined to fully analyze whole characteristic features of the samples. Cell culture study was also performed to demonstrate the biocompatibility properties of the fabricated scaffolds with human adipose tissue-derived mesenchymal stem cells (hAD-MSCs). 15 wt % PLA was selected as the control group and among all concentrations of CS and SA, groups containing 3 wt% CS and 3 wt% SA showed significantly superior and favorable features in printing quality. The research continued with these two scaffolds (3 wt% CS, and 3 wt% SA), which showed improved print quality when added to PLA. Overall, these results show that PLA/CS and PLA/SA 3D printed artificial patches have the potential to tissue engineering solutions to repair tympanic membrane perforation for people with hearing loss.
  • No Thumbnail Available
    PublicationMetadata only
    Kan nötrofil sayısındaki değişiklikler LDL ve östrojen düzeylerini etkiler
    (2022-11-01) KAHRAMAN, MERVE MERİÇ; ŞAHİN, ALİ; SEVİM M., ALTINOLUK T., KAHRAMAN M. M., Akgün T., ŞAHİN A., Özekici H. N., Fil A., Yıldız B., Yücetürk B., Lale E. N., et al.
    AMAÇ: Kada dolaşan nötrofillerın miktarı birçok hastalık ve inflamatuar durum için bir belirteç olacağı gibi, notrofilin kanda olması gerekenden fazla ya da az olması birçok sorunlu duruma da yol açmaktadır. Literatürde nötrofillerle ilgili yapılan birçok araştırma olmasına rağmen, nötropeni ya da notrofilik durumların kan östrojen ve kolesterol seviyeleri üzerine etkileri bilinmemektedir. Çalışmamızda nötropenik ve notrofilik duurmların kan LDL ve östrojen düzeyi üzerine olan etkileri fareler ve sağlıklı insan denekler üzerinde araştırılmıştır. YÖNTEM: Erkek (n=12) ve dişi (n=12) C57BL/6 farelerde nötropeni anti-nötrofil serumu (ANS), nötrofili ise iki farklı tipte rekombinant G-CSF ile oluşturulmuştur. Nötrofil sayısı ve LDL seviyeleri arasındaki ilişkiyi değerlendirmek için, erkek farelerde kan IL-17, G-CSF ve karaciğer HMG-CoA redüktaz enzim aktivitesi incelenmiştir. Cinsiyetlerin etkisini değerlendirmek içinse, erkek (n=12) ve kadın (n=13) insan deneklerden iki farklı zaman noktasında (menstrüasyon ve geç foliküler faz) kan örnekleri alınmıştır. BULGULAR: Nötrofil sayısındaki anlamlı bir artış ya da azalış, erkek farelerde toplam kan kolesterol düzeyini artırırken, dişi farelerde yalnızca artmış nötrofil sayısı (p=0.01) kan kolesterol düzeyinde anlamlı bir artışa neden olmuştur (p=0.05). Erkek farelerde görülen toplam kolesterol seviyesindeki artışın kaynağına bakıldığında bunun HDL'ye değil LDL'ye bağlı olduğu görülmüştür (p=0.001). Toplam kolesterol ile LDL ve nötrofil arasında sadece dişi farelerde (sırasıyla p=0.01, p=0.05) anlamlı bir korelasyon vardır, ancak bu korelasyon erkeklerde görülmemiştir. Fare verileriyle tutarlı olarak, kadın deneklerde menstrüel döngü sırasında östrojen seviyeleri arttığında (p=0.01), nötrofil sayılarının azaldığı (p=0.01) ve kolesterol düzeylerinin arttığı (p=0.05) görülmüştür. Farelerde ise G-CSF seviyelerinde fark bulunmazken, sadece ANS verilen farelerde IL-17'de artış gözlenmiştir (p=0.05). Hem ANS hem de filgrastim verilen farelerde HMG-CoA Redüktaz aktivitesinde kontrol grubuna göre bir düşüş gözlenmiştir (sırasıyla p=0.01, p=0.01). SONUÇ: Sonuç olarak, nötrofil sayısındaki anlamlı bir değişikliğin LDL düzeylerini doğrudan etkilediği gösterilmiştir. Bu etkileşim, ayrıca, adet döngüsü sırasında dalgalanan östrojen seviyeleri ile de olduğu gözlenmiştir.