Person: ŞAHİN, ALİ
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ŞAHİN
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ALİ
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Publication Open Access Levodopa-Loaded 3D-Printed Poly (Lactic) Acid/Chitosan Neural Tissue Scaffold as a Promising Drug Delivery System for the Treatment of Parkinson's Disease(MDPI, 2021-11-13) ŞAHİN, ALİ; Saylam, Ezgi; Akkaya, Yigit; Ilhan, Elif; Cesur, Sumeyye; Guler, Ece; Sahin, Ali; Cam, Muhammmet Emin; Ekren, Nazmi; Oktar, Faik Nuzhet; Gunduz, Oguzhan; Ficai, Denisa; Ficai, AntonParkinson's disease, the second most common neurodegenerative disease in the world, develops due to decreased dopamine levels in the basal ganglia. Levodopa, a dopamine precursor used in the treatment of Parkinson's disease, can be used as a drug delivery system. This study presents an approach to the use of 3D-printed levodopa-loaded neural tissue scaffolds produced with polylactic acid (PLA) and chitosan (CS) for the treatment of Parkinson's disease. Surface morphology and pore sizes were examined by scanning electron microscopy (SEM). Average pore sizes of 100-200 mu m were found to be ideal for tissue engineering scaffolds, allowing cell penetration but not drastically altering the mechanical properties. It was observed that the swelling and weight loss behaviors of the scaffolds increased after the addition of CS to the PLA. Levodopa was released from the 3D-printed scaffolds in a controlled manner for 14 days, according to a Fickian diffusion mechanism. Mesenchymal stem cells (hAD-MSCs) derived from human adipose tissue were used in MTT analysis, fluorescence microscopy and SEM studies and confirmed adequate biocompatibility. Overall, the obtained results show that PLA/CS 3D-printed scaffolds have an alternative use for the levodopa delivery system for Parkinson's disease in neural tissue engineering applications.Publication Open Access Production and characterization of PLA/HA/GO nanocomposite scaffold(2022-08-12) ŞAHİN, ALİ; GÜNDÜZ, OĞUZHAN; Oktay B., Ahlatcıoğlu Özerol E., Şahin A., Gunduz O., Ustundag C. B.© 2022 Wiley-VCH GmbH.Polylactic acid (PLA) composite nanofibers combined with hydroxyapatite (HA) and graphene oxide (GO) nanoparticles were produced by electrospinning to create excellent biodegradable and durable scaffolds to be used in tissue engineering. The properties of the pure PLA, PLA/HA, PLA/GO, and PLA/HA/GO nanocomposite scaffolds were analyzed in chemical, morphological, mechanical, and biocompatibility. Morphology and composition were investigated by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR), respectively. To predict the cytocompatibility of these scaffolds, HFF-1 cells were cultured and the respective cell adhesion and proliferation were investigated by fluorescence microscopy, SEM, and MTT assay. FTIR results showed the successful synthesis of HA and GO nanoparticles. SEM images showed that the PLA/HA/GO scaffold is ideal for cell attachment and proliferation in tissue regeneration. Mechanical test results showed that the tensile strength and elastic modulus of PLA nanofibers could be increased by adding 0,8 wt % HA and 0,4 wt % GO. The PLA/HA/GO scaffold exhibited the highest tensile strength of other scaffolds. MTT assay revealed that the PLA/HA/GO scaffold showed significantly high biocompatibility with 105 % cell viability. Therefore, PLA/HA/GO scaffold with 0,8 wt %HA and 0,4 wt %GO possessing high tensile strength as well as good cell proliferation is an excellent and versatile biomaterial for tissue engineering applications.Publication Open Access Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis(MDPI, 2021-04-28) ŞAHİN, ALİ; Ulag, Songul; Ilhan, Elif; Demirhan, Ramazan; Sahin, Ali; Yilmaz, Betul Karademir; Aksu, Burak; Sengor, Mustafa; Ficai, Denisa; Titu, Aurel Mihail; Ficai, Anton; Gunduz, OguzhanIn this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa, and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus, but for P. aeruginosa, more studies should be performed.Publication Open Access 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering(MDPI, 2020-08-29) ŞAHİN, ALİ; Cakmak, Abdullah M.; Unal, Semra; Sahin, Ali; Oktar, Faik N.; Sengor, Mustafa; Ekren, Nazmi; Gunduz, Oguzhan; Kalaskar, Deepak M.Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (similar to 300 mu m) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.Publication Open Access Production of 3D Printed Bi-Layer and Tri-Layer Sandwich Scaffolds with Polycaprolactone and Poly (vinyl alcohol)-Metformin towards Diabetic Wound Healing(2022-12-01) ŞAHİN, ALİ; Harmanci S., Dutta A., Cesur S., Sahin A., Gunduz O., Kalaskar D. M., Ustundag C. B.Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by impaired insulin secretion, sensitivity, and hyperglycemia. Diabetic wounds are one of the significant complications of T2DM owing to its difficulty in normal healing, resulting in chronic wounds. In the present work, PCL/PVA, PCL/PVA/PCL, and metformin-loaded, PCL/PVA-Met and PCL/PVA-Met/PCL hybrid scaffolds with different designs were fabricated using 3D printing. The porosity and morphological analysis of 3D-printed scaffolds were performed using scanning electron microscopy (SEM). The scaffolds’ average pore sizes were between 63.6 ± 4.0 and 112.9 ± 3.0 µm. Molecular and chemical interactions between polymers and the drug were investigated with Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD). Mechanical, thermal, and degradation analysis of the scaffolds were undertaken to investigate the physico-chemical characteristics of the scaffolds. Owing to the structure, PCL/PVA/PCL sandwich scaffolds had lower degradation rates than the bi-layer scaffolds. The drug release of the metformin-loaded scaffolds was evaluated with UV spectrometry, and the biocompatibility of the scaffolds on fibroblast cells was determined by cell culture analysis. The drug release in the PCL/PVA-Met scaffold was sustained till six days, whereas in the PCL/PVA-Met/PCL, it continued for 31 days. In the study of drug release kinetics, PCL/PVAMet and PCL/PVA-Met/PCL scaffolds showed the highest correlation coefficients (R2 ) values for the first-order release model at 0.8735 and 0.889, respectively. Since the layered structures in the literature are mainly obtained with the electrospun fiber structures, these biocompatible sandwich scaffolds, produced for the first time with 3D-printing technology, may offer an alternative to existing drug delivery systems and may be a promising candidate for enhancing diabetic wound healing.