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ŞAHİN, ALİ

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ŞAHİN

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ALİ

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2022 - 20243
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Subject: Clinical Medicine (MED) ×

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Now showing 1 - 3 of 3
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    In vitro evaluation of biomaterials for neural tissue engineering
    (Elsevier Science, Oxford/Amsterdam , 2023-04-01) ŞAHİN, ALİ; YILMAZ, BETÜL; Şahin A., Çıkı B., Yılmaz B.
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    Combination of second-generation proteasome inhibitor carfilzomib with bortezomib in four different breast cancer cell lin
    (2022-01-01) YILMAZ GÖLER, AYŞE MİNE; ŞAHİN, ALİ; YILMAZ, BETÜL; Altundag E. M., Yilmaz A. M., Sahin A., Yilmaz B.
    Background: Proteasome inhibitors target different pathways in cells and therefore are promising drugs in cancer therapy. The use of these inhibitors is approved mainly in hematological cancers, and recently many clinical trials and preclinical studies have been conducted on efficacy in solid tumors. Carfilzomib is a second-generation inhibitor and was developed to decrease the side effects of bortezomib. Although there are many valid therapies for breast cancer, resistance and recurrence are inevitable in many cases and the proteasomal system plays an important role in related pathways. Objective: This study is a preliminary work to evaluate the combined effects of bortezomib and carfilzomib in four different breast cancer cells. Methods: MDA-MB-231, MCF-7, UACC-2087, and SKBR-3 cell lines were used. Cell viability was determined using bortezomib and carfilzomib alone and in combination. Combination effect values were determined using the Chou-Talalay method. Apoptosis, proteasome activity, cleaved PARP, and HSP70 expressions were analyzed in the determined doses. Results: The response to the combination of the two inhibitors was different in four cell lines. Apoptosis was significantly higher in combination groups compared to carfilzomib in three cell lines except for SKBR-3, and higher in the combination group compared to bortezomib only in UACC-2087. Combination decreased cleaved PARP levels in MDA-MB-231 and MCF-7 and increased SKBR-3 compared to bortezomib. HSP70 levels decreased in combination with UACC-2087 and SKBR-3 compared to carfilzomib. Conclusion: Taken together, the combination of the two inhibitors was more apoptotic compared to carfilzomib and apoptosis was higher only in UACC-2087 compared to bortezomib. This apoptosis data can not be directly correlated to the degree of proteasome inhibition, PARP cleavage, and HSP70 response.
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    Electrospun Amygdalin and Inula helenium Extract-loaded Polylactic acid (PLA)/Polyvinylpyrrolidone (PVP) Nanofibrous Patches for Colon Cancer Treatment: Fabrication, Characterization and Antitumour Effect Results
    (2024-07-01) ŞAHİN, ALİ; Sulutas R. B., Cesur S., Seyhan S. A., Alkaya D. B., Şahin A., Ekren N., Gunduz O.
    in this study, 75PLA/25PVP nanofibrous loaded with amygdalin (AMG) andInula helenium(I.H) extract were produced by electrospinning method in order to prevent local colon cancer recurrence, and the effect of the produced nanofibrous on the HCT-116cell line was examined in vitro. When the scanning electron microscopy (SEM) images were examined, the fiber diameter of the 75PLA/25PVP group was measured as 443.74±79nm, but the addition ofI.Hand AMG caused a thickening effect on the fiber diameters. Drug release results show the controlled release period of 75PLA/25PVP/I.Hand 75PLA/25PVP/AMG loaded nanofibrous patches within 180h. Slowly degrading nanofibrous remained durable for up to 25 days. When the in vitro cytotoxicity results were analyzed, all nanofibrous were found to be effective in inducing cytotoxicity against HCT-116 colon cancer cells. The most effective group is the group in whichI.Hextract and AMG drug are loaded onto the nanofibrous together. AMG drug andI.Hplant extract showed effects on the colon cancer cell line in in vitro experiments. Considering all the results I.H and AMG loaded nanofibrous can be implanted into solid tumor cells in order to reduce the risk of local recurrence of cancer after surgery in colon cancer.