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DULUNDU, ENDER

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2011 - 20122
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Author: ŞENER, GÖKSEL × Subject: cytokines ×

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    Protective Effects of Lycopene on Cerulein-Induced Experimental Acute Pancreatitis in Rats
    (ACADEMIC PRESS INC ELSEVIER SCIENCE, 2012) DULUNDU, ENDER; Ozkan, Erkan; Akyuz, Cebrail; Dulundu, Ender; Topaloglu, Umit; Sehirli, Ahmet Ozer; Ercan, Feriha; Sener, Goksel
    Background. The purpose of our study was to evaluate the protective effect of the strong antioxidant and anti-inflammatory agent, lycopene, on oxidative stress in a rat model of cerulein-induced acute edematous pancreatitis. Methods. Sprague-Dawley rats were pretreated with lycopene (50 mg/kg, i.p.) or saline 15 min before cerulein was given 20 mu g/kg (i.p.) at 1-h intervals within 4 h. Twelve hours after cerulein or saline injections, the animals were killed by decapitation. Blood samples were collected to analyze amylase, lipase, and proinflammatory cytokines (TNF-alpha and IL-1 beta). Pancreatic tissues were taken for the determination of tissue glutathione (GSH) and malondialdehyde (MDA) levels, Na+/K+-ATPase, and myeloperoxidase (MPO) activities. Tissue samples were also examined histologically. Results. Acute pancreatitis caused significant decrease in tissue GSH levels and Na+/K+-ATPase activity, while pancreatic MDA levels and MPO activity were increased. Furthermore, TNF-alpha, IL-1 beta, and amylase lipase levels were also significantly increased. On the other hand, lycopene pretreatment reserved all these biochemical indices as well as histopathologic alterations that were induced by cerulein. Conclusions. According to the results, lycopene protects the pancreatic tissues from oxidative damage induced by cerulein, and this effect possibly involves the inhibition of neutrophil infiltration and lipid peroxidation. These results suggest that high dietary intake of tomatoes may have protective effects against acute pancreatitis. (C) 2012 Elsevier Inc. All rights reserved.
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    Beneficial effects of alpha lipoic acid on cerulein-induced experimental acute pancreatitis in rats
    (TURKISH ASSOC TRAUMA EMERGENCY SURGERY, 2011) DULUNDU, ENDER; Bulut, Nuriye Esen; Ozkan, Erkan; Ekince, Osman; Dulundu, Ender; Topaloglu, Umit; Sehirli, Ahmet Ozer; Ercan, Feriha; Sener, Goksel
    BACKGROUND The present study aimed to determine the effects of alpha lipoic acid (ALA) on blood and tissue biochemical parameters, as well as tissue histopathology, in an experimental rat model of cerulein-induced acute pancreatitis (AP). METHODS Three groups consisting of eight rats each were used, as follows: Group I. controls; Group 2, cerulein-induced pancreatitis group treated with saline; and Group 3, cerulein-induced pancreatitis group treated with ALA. AP was induced by intraperitoneal administration of cerulein (20 mu g/kg) 4 times at I-hour intervals. The animals were decapitated 12 hours after the last dose of cerulein. Blood amylase, lipase, interleukin (IL)-1 beta, and tumor necrosis factor (TNF)-alpha levels, pancreas tissue glutathione (GSH) and malondialdehyde (MDA) levels, as well as myeloperoxidase (MPO) and Na+-K+-ATPase activity were measured. Pancreatic tissue samples were also evaluated histopathologically under a light microscope. RESULTS While plasma amylase, lipase, IL-1 beta, and TNF-alpha levels, and tissue MDA and MPO levels significantly increased in rats with cerulean-induced AP, tissue GSH and Na+-K+-ATPase activity significantly reduced. These changes were reversed and improved with ALA treatment. CONCLUSION Our findings suggest that ALA may significantly reduce morbidity and mortality by preventing organ dysfunction induced by free radicals in the pancreas.