Person: ŞİRVANCI, SERAP
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ŞİRVANCI
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SERAP
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Publication Metadata only Early pulmonary involvement in Niemann-Pick type B disease: Lung lavage is not useful(WILEY, 2005) KARADAĞ, BÜLENT TANER; Uyan, ZS; Karadag, B; Ersu, R; Kiyan, G; Kotiloglu, E; Sirvanci, S; Ercan, F; Dagli, T; Karakoc, F; Dagli, ENiemann-Pick disease (NPD) is a rare, autosomal-recessively inherited lipid storage disease which is characterized by intracellular deposition of sphingomyelin in various body tissues. The disease is heterogeneous and classified into six groups. Pulmonary parenchymal involvement may be a feature of several subtypes of NPD, including type B. Progressive pulmonary involvement in NPD type B is a major cause of morbidity and mortality It is usually diagnosed at older ages. Only a few cases with early pulmonary involvement have been reported. In this report, a patient with NPD type B, hospitalized with the diagnosis of pneumonia at age 3 months, is presented. Following treatment for pneumonia, she continued to have persistent respiratory symptoms and became oxygen-dependent. High-resolution computed tomography of the chest revealed diffuse interstitial changes. During follow-up, the patient developed hepatosplenomegaly. Lung, liver, and bone marrow biopsies showed characteristic findings for NPD. Biochemical studies also confirmed the diagnosis, and the sphingomyelinase enzyme level of the patient was low. Unilateral lung lavage was performed in order to decrease lipid storage as a treatment modality However, there was no clinical or radiological improvement. The patient died at age 15 months due to progressive respiratory failure. Pulmonary involvement is a rare entity in early childhood in patients with NPD type B, but should be considered in the differential diagnosis of persistent interstitial lung disease. It may cause progressive respiratory failure, but the treatment options remain limited.Publication Open Access The effects of alpha-lipoic acid (ALA) on the urinary bladder injury in rats exposed to chronic stress: a histochemical study(2022-01-01) ŞİRVANCI, SERAP; Yurdakul N., Cikler E., Toklu H. Z. , ŞİRVANCI S.Objective: In the present study, we aimed to investigate the morphological and biochemical effects of alpha-lipoic acid (ALA) on bladder injury caused by water avoidance stress (WAS) and to show its effect on the number of degranulated mast cells, which increase after stress.Materials and Methods: Wistar albino rats were subjected to WAS and the animals in the treatment group were injected ALA. After the urinary bladder tissues were subjected to routine tissue processing, hematoxylin-eosin staining and periodic acid-Schiff reaction were applied to observe general morphology and acidic toluidine blue method to investigate mast cells. Biochemical assessments of malondialdehyde (MDA) and glutathione (GSH) were also obtained. Transmission electron microscope was used for the ultrastructural, and scanning electron microscope for the topographical analyses. Results: The experiments showed that chronic stress caused injury in the bladder, increased degranulated and total number of mast cells and decreased GSH and increased MDA levels. ALA treatment after WAS ameliorated bladder injury in most areas, decreased degranulated and total mast cell number and increased GSH and decreased MDA levels.Conclusion: It was concluded that ALA can be a useful agent in the treatment of interstitial cystitis.Publication Metadata only Anti-inflammatory properties of brilliant blue G on chronic unpredictable mild stress-induced changes in rat hippocampus(ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017) ARICIOĞLU, FEYZA; Aricioglu, F.; Bastaskin, T.; Kandemir, C.; Sirvanci, S.; Ozkartal, C.; Utkan, T.Publication Metadata only The neuroprotective and anti-apoptotic effects of melatonin on hemolytic hyperbilirubinemia-induced oxidative brain damage(WILEY, 2016) MEMİŞOĞLU, ASLI; Pazar, Asilay; Kolgazi, Meltem; Memisoglu, Asli; Bahadir, Elif; Sirvanci, Serap; Yaman, Akan; Yegen, Berrak C.; Ozek, ErenMelatonin exerts protection in several inflammatory and neurodegenerative disorders. To investigate the neuroprotective effects of melatonin in an experimental hemolysis-induced hyperbilirubinemia, newborn Sprague-Dawley rats (25-40 g, n = 72) were injected with phenylhydrazine hydrochloride (PHZ; 75 mg/kg) and the injections were repeated at the 24th hour. Rats were treated with saline or melatonin (10 mg/kg) 30 min before the first and second PHZ injections and 24 h after the 2nd PHZ injections. Control rats (n = 24) were injected with saline, but not PHZ. At sixth hours after the last injections of saline or melatonin, all rats were decapitated. Tumor necrosis factor (TNF)-alpha, IL-1 beta, IL-10 and brain-derived neurotrophic factor (BDNF) and S100B levels in the plasma were measured. Brain tissue malondialdehyde (MDA), glutathione (GSH) levels and myeloperoxidase (MPO) activities were measured, and brain tissues were evaluated for apoptosis by TUNEL method. In the saline-treated PHZ group, hemoglobin, hematocrit levels were reduced, and total/direct bilirubin levels were elevated when compared to control group. Increased plasma TNF-alpha, IL-1 beta levels, along with decreased BDNF, S100B and IL-10 values were observed in the saline-treated PHZ group, while these changes were all reversed in the melatonin-treated group. Increased MDA levels and MPO activities in the brain tissues of saline-treated hyperbilirubinemic rats, concomitant with depleted brain GSH stores, were also reversed in the melatonin-treated hyperbilirubinemic rats. Increased TUNEL(+) cells in the hippocampus of saline-treated PHZ group were reduced by melatonin treatment. Melatonin exerts neuroprotective and anti-apoptotic effects on the oxidative neuronal damage of the newborn rats with hemolysis and hyperbilirubinemia.Publication Metadata only Harmane suppresses microglial neuroinflammatory response and induce antidepressant-like effect in rats(ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017) ARICIOĞLU, FEYZA; Aricioglu, F.; Arkan, G.; Kandemir, C.; Sirvanci, S.; Ozkartal, C.; Utkan, T.Publication Open Access Testicular apoptosis and proliferation in relation to body mass index and m-TESE success in nonobstructive azoospermic men(2023-04-01) ŞİRVANCI, SERAP; Kuntsal Dertsiz E., Kadıoğlu A., Şirvancı S.Male infertility occurs due to the disruption of the balance of proliferation and apoptosis in the testicular tissue. It is important toshow the effect of increased body mass index, which is one of the factors that disrupts this balance of apoptosis and proliferation,not only at the blood hormone level but also at the tissue level. For this reason, the present study is aimed at demonstrating therelation between body mass index and cell turnover in the testis using immunohistochemical methods. In the present study,patients were grouped as normal, overweight and obese, and as m-TESE positive and negative. The sperm retrieval rate withmicrosurgical testicular sperm extraction (m-TESE) was 41.67%. Histological diagnosis of testicular tissues was made withhematoxylin and eosin and Masson’s trichrome staining. Apoptosis and proliferation in the testicular tissue were demonstratedby TUNEL and PCNA immunohistochemical methods, respectively. It was concluded that BMI had no significant effect onreproductive hormone profile (FSH, LH, and testosterone), m-TESE success, apoptosis, and proliferation in testicular tissue innonobstructive azoospermic men. In addition TUNEL positivity and proliferative index was found to be significantly correlatedwith testicular histology and m-TESE outcome.Publication Open Access Investigation of neurogenesis in kindled wistar and genetic absence epilepsy rats(2022-09-01) ŞİRVANCI, SERAP; KAYA, ÖZLEM TUĞÇE; Kandemir C., Yavuz M., Karakaya F. B. , Kaya Ö. T. , Onat F., Şirvancı S.Publication Metadata only Demonstration of Doublecortin Protein, a Neurogenesis Marker, at the Electron Microscopic Level(AVES PRESS LTD, 2017) KAYA, ÖZLEM TUĞÇE; Kaya, Ozlem Tugce Cilingir; Moore, Cynthia; Meshul, Charles; Sirvanci, SerapObjective: Demonstration of newly born neurons in adult brains is an important issue in terms of elucidating neurogenesis. In our study, we aimed to develop an appropriate pre-embedding microwave labeling method for the newly born neuronal marker doublecortin (DCX) protein in the hippocampal dentate gyrus (DG) region. Methods: Brains were obtained from 10-week-old C57BJ/6J male mice by perfusion fixation. Vibratome sections from brain tissues were labeled with an anti-DCX antibody using a pre-embedding microwave method. Sections stained with 3.3'-diaminobenzidine (DAB) were prepared for electron microscopic (EM) analyses using a microwave. After embedding in Epon, thin sections were obtained, observed under an electron microscope, and photographed for morphological assessments. Results: DCX labeling performed using the pre-embedding microwave method was specifically demonstrated at both light and electron microscopic levels. DCX-positive cells were localized at the subgranular zone and granular layer. Electron microscopic observations showed that DCX immunoreactivity was positive at the axons, dendrites, and somata. Conclusion: We demonstrated that the existence of DCX can be determined using the pre-embedding microwave labeling as an immunoelectron method in the DG region. Our study provides a basis for further studies on neurogenesis aiming to show DCX-immunoreactive cells using the pre-embedding DAB labeling method at the electron microscopic level.Publication Metadata only Primer siliyer diskinezi tanısında transmisyon elektron mikroskopi(Türkiye Klinikleri, 2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; ŞİRVANCI S., KAYA Ö. T.Primer siliyer diskinezi (PSD), siliya fonksiyon bozukluğunun neden olduğu, çoğunlukla otozomal resesif geçişli, klinik ve genetik olarak heterojen bir hastalıktır. PSD klinik fenotipi değişkendir. Üst ve alt solunum sistemi, üreme organları, kalp ve siliyaların bulunduğu diğer sistemleri tutar. Zamanında doğan PSD'li bebeklerin yaklaşık %80'i, doğum sonrası 24 saat içinde solunum sıkıntısı kliniği gösterir ve oksijen desteği gerektirir. Sonraki dönemde tekrarlayan pnömoni veya bronşit yaygın görülür. Kronik, efüzyonlu tekrarlayan orta kulak iltihabı, özellikle yaşamın ilk yılında, PSD'li çocukların en az %80'ini etkiler. Hastaların yaklaşık %80'inde yıl boyu devam eden burun tıkanıklığı ve kronik sinüzit vardır. Situs inversus totalis daha sık olmak üzere bir dizi organ lateralite kusuru görülür. Erkeklerde infertilite, kadınlarda ektopik gebelik sıktır. Bazı durumlarda, PSD'nin polikistik böbrek, hidrosefali, polispleni gibi nadir ve olağandışı bozukluklarla ilişkili olduğu gösterilmiştir. Son zamanlarda genetik çalışmaların hızlanmasıyla yapılan çalışmalar, farklı genlerdeki mutasyonların değişken fenotiplere yol açtığını göstermektedir. Bununla ilgili vaka serileri mevcuttur.Publication Open Access Effect of perinatal and postnatal thiamine deficiency on auditory pathway of the Wistar-Albino rats(2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; Gür Ö. E., Yılmaz N. D. S., Ensari N., Senirli R. T., GÜLMEZ Z. D., KAYA Ö. T., ŞİRVANCI S., Danışman B., DERİN N., Yılmaz M. D.© 2022 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-FacialObjective: In this study, we created an animal model to demonstrate the effects of thiamine on the hearing pathways of new-borns during pregnancy and lactation by inducing a dietary thiamine deficiency in the mother. Methods: The study included 16 female Wistar albino rats. The animals were separated into four groups and provided the appropriate amounts of dietary thiamine according to their groups during pre-pregnancy, pregnancy, and lactation periods. Three pups from each mother were included in the study, and 12 pups were selected from each group. On the fortieth day after birth, the auditory pathways of 48 pups in the 4 groups were examined electro physiologically and ultra-structurally. Results: In Group N-N, morphology of hair cells stereocilia degeneration was not obtained in all turns of cochlea. In Group N-T, Inner Hair Cells (IHCs) and Outher Hair Cells (OHCs) stereocilia didn\"t show degeneration in all turns of cochlea but had rupture inrows of HCs stereocilia. In group T-N IHCs stereocilia less degeneration was observed in all turns of cochlea. OHC stereocilia partial loss was observed only in basal turn of cochlea. In Group T-T IHCs stereocilia was observed less degeneration and rupture in all turns of cochlea. Conclusion: Thiamine is vital for the development of cochlear hair cells during both prenatal and postnatal periods. Even partial deficiency of thiamine causes significant degeneration to the auditory pathway. Level of evidence: The level of evidence of this article is 5. This article is an experimental animal and laboratory study.