Person: ŞİRVANCI, SERAP
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ŞİRVANCI
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SERAP
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Publication Metadata only Anti-inflammatory properties of brilliant blue G on chronic unpredictable mild stress-induced changes in rat hippocampus(ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017) ARICIOĞLU, FEYZA; Aricioglu, F.; Bastaskin, T.; Kandemir, C.; Sirvanci, S.; Ozkartal, C.; Utkan, T.Publication Metadata only The neuroprotective and anti-apoptotic effects of melatonin on hemolytic hyperbilirubinemia-induced oxidative brain damage(WILEY, 2016) MEMİŞOĞLU, ASLI; Pazar, Asilay; Kolgazi, Meltem; Memisoglu, Asli; Bahadir, Elif; Sirvanci, Serap; Yaman, Akan; Yegen, Berrak C.; Ozek, ErenMelatonin exerts protection in several inflammatory and neurodegenerative disorders. To investigate the neuroprotective effects of melatonin in an experimental hemolysis-induced hyperbilirubinemia, newborn Sprague-Dawley rats (25-40 g, n = 72) were injected with phenylhydrazine hydrochloride (PHZ; 75 mg/kg) and the injections were repeated at the 24th hour. Rats were treated with saline or melatonin (10 mg/kg) 30 min before the first and second PHZ injections and 24 h after the 2nd PHZ injections. Control rats (n = 24) were injected with saline, but not PHZ. At sixth hours after the last injections of saline or melatonin, all rats were decapitated. Tumor necrosis factor (TNF)-alpha, IL-1 beta, IL-10 and brain-derived neurotrophic factor (BDNF) and S100B levels in the plasma were measured. Brain tissue malondialdehyde (MDA), glutathione (GSH) levels and myeloperoxidase (MPO) activities were measured, and brain tissues were evaluated for apoptosis by TUNEL method. In the saline-treated PHZ group, hemoglobin, hematocrit levels were reduced, and total/direct bilirubin levels were elevated when compared to control group. Increased plasma TNF-alpha, IL-1 beta levels, along with decreased BDNF, S100B and IL-10 values were observed in the saline-treated PHZ group, while these changes were all reversed in the melatonin-treated group. Increased MDA levels and MPO activities in the brain tissues of saline-treated hyperbilirubinemic rats, concomitant with depleted brain GSH stores, were also reversed in the melatonin-treated hyperbilirubinemic rats. Increased TUNEL(+) cells in the hippocampus of saline-treated PHZ group were reduced by melatonin treatment. Melatonin exerts neuroprotective and anti-apoptotic effects on the oxidative neuronal damage of the newborn rats with hemolysis and hyperbilirubinemia.Publication Metadata only Harmane suppresses microglial neuroinflammatory response and induce antidepressant-like effect in rats(ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2017) ARICIOĞLU, FEYZA; Aricioglu, F.; Arkan, G.; Kandemir, C.; Sirvanci, S.; Ozkartal, C.; Utkan, T.Publication Metadata only Demonstration of Doublecortin Protein, a Neurogenesis Marker, at the Electron Microscopic Level(AVES PRESS LTD, 2017) KAYA, ÖZLEM TUĞÇE; Kaya, Ozlem Tugce Cilingir; Moore, Cynthia; Meshul, Charles; Sirvanci, SerapObjective: Demonstration of newly born neurons in adult brains is an important issue in terms of elucidating neurogenesis. In our study, we aimed to develop an appropriate pre-embedding microwave labeling method for the newly born neuronal marker doublecortin (DCX) protein in the hippocampal dentate gyrus (DG) region. Methods: Brains were obtained from 10-week-old C57BJ/6J male mice by perfusion fixation. Vibratome sections from brain tissues were labeled with an anti-DCX antibody using a pre-embedding microwave method. Sections stained with 3.3'-diaminobenzidine (DAB) were prepared for electron microscopic (EM) analyses using a microwave. After embedding in Epon, thin sections were obtained, observed under an electron microscope, and photographed for morphological assessments. Results: DCX labeling performed using the pre-embedding microwave method was specifically demonstrated at both light and electron microscopic levels. DCX-positive cells were localized at the subgranular zone and granular layer. Electron microscopic observations showed that DCX immunoreactivity was positive at the axons, dendrites, and somata. Conclusion: We demonstrated that the existence of DCX can be determined using the pre-embedding microwave labeling as an immunoelectron method in the DG region. Our study provides a basis for further studies on neurogenesis aiming to show DCX-immunoreactive cells using the pre-embedding DAB labeling method at the electron microscopic level.Publication Open Access Antidepressant-like Effects Induced by Chronic Blockade of the Purinergic 2X7 Receptor through Inhibition of Non-like Receptor Protein 1 Inflammasome in Chronic Unpredictable Mild Stress Model of Depression in Rats(KOREAN COLL NEUROPSYCHOPHARMACOLOGY, 2019-05-31) ARICIOĞLU, FEYZA; Aricioglu, Feyza; Ozkartal, Ceren Sahin; Bastaskin, Tugce; Tuzun, Erdem; Kandemir, Cansu; Sirvanci, Serap; Kucukali, Cem Ismail; Utkan, TijenObjective: Purinergic 2X7 receptor (P2X7R) activation is known to be involved in pathogenesis of depression. Our aims were to investigate P2X7R-activated inflammasome pathways in parallel with induction of depression and to test the antidepressant-like effects of the selective P2X7R antagonist Brilliant Blue G (BBG) in a rat model of chronic unpredictable mild stress (CUMS). Methods: Male Wistar albino rats were divided into control, CUMS, CUMS+BBG25 (25 mg/kg/day) and CUMS+BBG50 (50 mg/kg/day) groups (n=10 for each group). Various stressors were applied to rats for 6 weeks to establish the CUMS model and daily BBG treatment was started at the end of 3rd week. Sucrose preference test and forced swim test (FST) were performed to assess antidepressant-like effects. Brain samples were obtained for real-time polymerase chain reaction and immunohistochemistry analysis. Results: In FST, duration of immobility was reduced in the CUMS+BBG50 group. Also, BBG treatment significantly enhanced sucrose preference. While NLRP3 gene expression levels were unchanged in rats exposed to the CUMS protocol, expression levels of other inflammasome pathway factors NLRP1, caspase-1, ASC, NF-kappa B, IL-1 beta, IL-6 and P2X7R were increased. BBG treatment reduced expression levels of these factors. Likewise, Iba-1 and GFAP immunoreactivities were enhanced by the CUMS protocol and this action was reversed by BBG treatment. Conclusion: Chronic administration of BBG in CUMS model results in antidepressant-like activity in a dose dependent manner. Molecular and histological results show that these effects might be at least partially related to the suppression of inflammasome-related neuroinflammatory responses and suggest involvement of NLRP1 in depression.Publication Metadata only Neuroprotective Effect of Erythropoietin on PhenylhydrazineInduced Hemolytic Hyperbilirubinemia in Neonatal Rats(SPRINGER/PLENUM PUBLISHERS, 2017) MEMİŞOĞLU, ASLI; Memisoglu, Asli; Kolgazi, Meltem; Yaman, Akan; Bahadir, Elif; Sirvanci, Serap; Yegen, Berrak C.; Ozek, ErenNeonatal unconjugated hyperbilirubinemia might cause severe bilirubin neurotoxicity in especially hemolytic conditions. The study aimed to elucidate the potential neuroprotective effects of erythropoietin (EPO) in hemolysis-induced hyperbilirubinemia. In newborn rats, hyperbilirubinemia secondary to hemolysis was induced by injecting with phenylhydrazine hydrochloride (PHZ) and rats were injected with either vehicle or EPO. At 54th hour of the PHZ injection, rats were decapitated. Serum levels of TNF-alpha, IL-1 beta, IL-10, brain-derived neurotrophic factor (BDNF) and S100-B and brain malondialdehyde, glutathione levels and myeloperoxidase activities were measured. TUNEL staining and NF-kappa B expression were evaluated. As compared to control pups, in vehicle-treated PHZ group, TNF-alpha and IL- 1 beta levels, malondialdehyde level and myeloperoxidase activity were increased with concomitant decreases in IL-10 and glutathione. All EPO regimens reversed PHZ-induced alterations in IL-10, TNFa, malondialdehyde and glutathione levels. Three-daytreatment abolished increases in myeloperoxidase activity and IL-1 beta levels, while BDNF and S100-B were elevated. Increased TUNEL (+) cells and NF-kappa B expressions in the brain of PHZ group were reduced in the 3-day-treated group. EPO exerted anti- inflammatory effects on PHZinduced neural damage in newborn rats, while the neuroprotection was more obvious when the treatments were repeated successively. The results suggest that EPO treatment may have a therapeutic potential in supporting neuroplasticity in the hyperbilirubinemic neonates.Publication Metadata only Synaptic organization of the rat thalamus: a quantitative study(SPRINGER-VERLAG ITALIA SRL, 2011) ONAT, FİLİZ; Cavdar, Safiye; Hacioglu, Husniye; Sirvanci, Serap; Keskinoz, Elif; Onat, FilizFirst-order thalamic nuclei receive driving afferents from ascending pathways and transmit processed information to the cortex. Higher-order thalamic nuclei receive driver messages from layer 5 of cortex and transmit information from one cortical area to the other. The different types of axon terminals RL (round vesicles, large terminals), RS (round vesicles, small terminals) and F (flattened vesicles) and their synaptic junctions have been here compared in three first-order (ventrobasal, lateral geniculate and anteroventral) and three higher-order (posterior, lateral posterior and mediodorsal) thalamic nuclei of the rat. In the present study, the higher-order relays differ from first-order relays as in the cat, in having fewer driver terminals (RL) and synapses than do the first-order relays. However, the F terminals showed opposite ratios in the first versus higher-order thalamic nuclei. The majority of the terminals in all thalamic nuclei studied were RS terminals. The area measurements of the three types of terminals and synaptic lengths showed no significant differences between first and higher-order nuclei. The driver inputs represent the minority and the modulatory inputs represent the majority of the terminals and synapses in all thalamic nuclei. In conclusion, there is a relative paucity of driver inputs, whereas modulatory inputs establish more numerous synapses to achieve finer modulation.Publication Metadata only Effect of perinatal biotin deficiency on auditory pathway of the Wistar-Albino rats(TAYLOR & FRANCIS LTD, 2019) KAYA, ÖZLEM TUĞÇE; Yllmaz, Nevreste Didem Sonbay; Gur, Ozer Erdem; Ensari, Nuray; Bulut, Erdogan; Kaya, Ozlem Tugce; Sirvanci, Serap; Danisman, Betul; Derin, Narin; Gezgin, Bahri; Aygener, Nurdan; Yilmaz, Mustafa DenizAim: Severe biotin deficiency associated with biotinidase enzyme deficiency in newborns is seen as severe neurological problems and hearing loss. However, the effect on the infant of deficiencies in the maternal diet during pregnancy are not clear. Material and methods: The study included 16 female Wistar albino rats and 4 male Wistar albino rats, that were mated and then the females were separated into 4 groups. At 40 days after the birth, 3 pups were selected from each group, and these 12 pups were evaluated with DPOAE and ABR electrophysiologically and the cochlea was examined ultrastructurally with electron microscopy. Results: In the DPOAE evaluation, At 8000 and 11,000 Hz, the signal-noise ratios in the B-N and B-B groups were statistically significantly higher (p < .05). In ABR, lengthening of the latency periods was determined in all the waves at both 8 and 16 kHz in the B-B group. When the IPL periods were examined, lengthening in IPL 1-5 was statistically significant in the B-B group only at 8 kHz. Conclusions: Biotin can be said to have an effect on hearing pathways. However, specifically where on the hearing pathways that biotin is involved has not been clarified.Publication Metadata only Nesfatin-1 alleviates gastric damage via direct antioxidant mechanisms(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015) YEGEN, BERRAK; Kolgazi, Meltem; Cantali-Ozturk, Cigdem; Deniz, Rabia; Ozdemir-Kumral, Zarife Nigar; Yuksel, Meral; Sirvanci, Serap; Yegen, Berrak C.Background: Indomethacin is a nonsteroidal anti-inflammatory drug, which is known to produce serious side effects, causing ulcerative lesions. Nesfatin-1, a newly identified anorexigenic peptide, was recently shown to have neuroprotective effects. The aim of the study was to investigate the anti-inflammatory effects of nesfatin-1 on indomethacin-induced gastric ulcer. Materials and methods: After a 24-h starvation period, ulcer was induced in Sprague-Dawley rats by subcutaneous administration of indomethacin (25 mg/kg), whereas control group received vehicle. Fifteen minutes after ulcer induction, rats were treated with either saline or nesfatin-1 (0.1, 0.3, or 1 mu g/kg, intraperitoneally). At the fourth hour, all rats were decapitated and their trunk blood was collected for tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 measurements. Stomach samples were examined microscopically and analyzed for myeloperoxidase (MPO) activity, malondialdehyde (MDA), glutathione (GSH), luminol-, and lucigenin-enhanced chemiluminescence (CL) levels. Results: Ulcer induction increased serum TNF-alpha; and IL-6 levels, gastric CL and MDA levels and MPO activity but decreased gastric GSH content (P < 0.05-0.001). On the other hand, 0.1 mu g/kg dose of nesfatin-1 reduced microscopic and macroscopic damage scores, decreased MPO activity and MDA levels, CL and IL-6 levels, whereas gastric GSH was replenished (P < 0.01). However, indomethacin-induced increase in TNF-alpha level was abolished at only 1 mu g/kg dose of nesfatin-1 (P < 0.01). Conclusions: Nesfatin-1 alleviated indomethacin-induced gastric injury, suggesting that the anti-inflammatory and gastroprotective effects of nesfatin-1 on oxidative gastric damage could be implemented by supporting the balance in oxidant and antioxidant systems while inhibiting the generation of pro-inflammatory mediators. (C) 2015 Elsevier Inc. All rights reserved.Publication Metadata only Antidepressant effect of ketamine in chronic unpredictable mild stress model: Involvement of nod-like protein inflammasome driven pathway(ELSEVIER, 2019) ARICIOĞLU, FEYZA; Aricioglu, F.; Yalcinkaya, C.; Ozkartal, C. Sahin; Tuzun, E.; Kucukali, C.; Kandemir, C.; Sirvanci, S.; Utkan, T.