Person: ŞİRVANCI, SERAP
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ŞİRVANCI
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SERAP
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Publication Open Access The effects of alpha-lipoic acid (ALA) on the urinary bladder injury in rats exposed to chronic stress: a histochemical study(2022-01-01) ŞİRVANCI, SERAP; Yurdakul N., Cikler E., Toklu H. Z. , ŞİRVANCI S.Objective: In the present study, we aimed to investigate the morphological and biochemical effects of alpha-lipoic acid (ALA) on bladder injury caused by water avoidance stress (WAS) and to show its effect on the number of degranulated mast cells, which increase after stress.Materials and Methods: Wistar albino rats were subjected to WAS and the animals in the treatment group were injected ALA. After the urinary bladder tissues were subjected to routine tissue processing, hematoxylin-eosin staining and periodic acid-Schiff reaction were applied to observe general morphology and acidic toluidine blue method to investigate mast cells. Biochemical assessments of malondialdehyde (MDA) and glutathione (GSH) were also obtained. Transmission electron microscope was used for the ultrastructural, and scanning electron microscope for the topographical analyses. Results: The experiments showed that chronic stress caused injury in the bladder, increased degranulated and total number of mast cells and decreased GSH and increased MDA levels. ALA treatment after WAS ameliorated bladder injury in most areas, decreased degranulated and total mast cell number and increased GSH and decreased MDA levels.Conclusion: It was concluded that ALA can be a useful agent in the treatment of interstitial cystitis.Publication Open Access Testicular apoptosis and proliferation in relation to body mass index and m-TESE success in nonobstructive azoospermic men(2023-04-01) ŞİRVANCI, SERAP; Kuntsal Dertsiz E., Kadıoğlu A., Şirvancı S.Male infertility occurs due to the disruption of the balance of proliferation and apoptosis in the testicular tissue. It is important toshow the effect of increased body mass index, which is one of the factors that disrupts this balance of apoptosis and proliferation,not only at the blood hormone level but also at the tissue level. For this reason, the present study is aimed at demonstrating therelation between body mass index and cell turnover in the testis using immunohistochemical methods. In the present study,patients were grouped as normal, overweight and obese, and as m-TESE positive and negative. The sperm retrieval rate withmicrosurgical testicular sperm extraction (m-TESE) was 41.67%. Histological diagnosis of testicular tissues was made withhematoxylin and eosin and Masson’s trichrome staining. Apoptosis and proliferation in the testicular tissue were demonstratedby TUNEL and PCNA immunohistochemical methods, respectively. It was concluded that BMI had no significant effect onreproductive hormone profile (FSH, LH, and testosterone), m-TESE success, apoptosis, and proliferation in testicular tissue innonobstructive azoospermic men. In addition TUNEL positivity and proliferative index was found to be significantly correlatedwith testicular histology and m-TESE outcome.Publication Open Access Investigation of neurogenesis in kindled wistar and genetic absence epilepsy rats(2022-09-01) ŞİRVANCI, SERAP; KAYA, ÖZLEM TUĞÇE; Kandemir C., Yavuz M., Karakaya F. B. , Kaya Ö. T. , Onat F., Şirvancı S.Publication Metadata only Primer siliyer diskinezi tanısında transmisyon elektron mikroskopi(Türkiye Klinikleri, 2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; ŞİRVANCI S., KAYA Ö. T.Primer siliyer diskinezi (PSD), siliya fonksiyon bozukluğunun neden olduğu, çoğunlukla otozomal resesif geçişli, klinik ve genetik olarak heterojen bir hastalıktır. PSD klinik fenotipi değişkendir. Üst ve alt solunum sistemi, üreme organları, kalp ve siliyaların bulunduğu diğer sistemleri tutar. Zamanında doğan PSD'li bebeklerin yaklaşık %80'i, doğum sonrası 24 saat içinde solunum sıkıntısı kliniği gösterir ve oksijen desteği gerektirir. Sonraki dönemde tekrarlayan pnömoni veya bronşit yaygın görülür. Kronik, efüzyonlu tekrarlayan orta kulak iltihabı, özellikle yaşamın ilk yılında, PSD'li çocukların en az %80'ini etkiler. Hastaların yaklaşık %80'inde yıl boyu devam eden burun tıkanıklığı ve kronik sinüzit vardır. Situs inversus totalis daha sık olmak üzere bir dizi organ lateralite kusuru görülür. Erkeklerde infertilite, kadınlarda ektopik gebelik sıktır. Bazı durumlarda, PSD'nin polikistik böbrek, hidrosefali, polispleni gibi nadir ve olağandışı bozukluklarla ilişkili olduğu gösterilmiştir. Son zamanlarda genetik çalışmaların hızlanmasıyla yapılan çalışmalar, farklı genlerdeki mutasyonların değişken fenotiplere yol açtığını göstermektedir. Bununla ilgili vaka serileri mevcuttur.Publication Open Access Effect of perinatal and postnatal thiamine deficiency on auditory pathway of the Wistar-Albino rats(2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; Gür Ö. E., Yılmaz N. D. S., Ensari N., Senirli R. T., GÜLMEZ Z. D., KAYA Ö. T., ŞİRVANCI S., Danışman B., DERİN N., Yılmaz M. D.© 2022 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-FacialObjective: In this study, we created an animal model to demonstrate the effects of thiamine on the hearing pathways of new-borns during pregnancy and lactation by inducing a dietary thiamine deficiency in the mother. Methods: The study included 16 female Wistar albino rats. The animals were separated into four groups and provided the appropriate amounts of dietary thiamine according to their groups during pre-pregnancy, pregnancy, and lactation periods. Three pups from each mother were included in the study, and 12 pups were selected from each group. On the fortieth day after birth, the auditory pathways of 48 pups in the 4 groups were examined electro physiologically and ultra-structurally. Results: In Group N-N, morphology of hair cells stereocilia degeneration was not obtained in all turns of cochlea. In Group N-T, Inner Hair Cells (IHCs) and Outher Hair Cells (OHCs) stereocilia didn\"t show degeneration in all turns of cochlea but had rupture inrows of HCs stereocilia. In group T-N IHCs stereocilia less degeneration was observed in all turns of cochlea. OHC stereocilia partial loss was observed only in basal turn of cochlea. In Group T-T IHCs stereocilia was observed less degeneration and rupture in all turns of cochlea. Conclusion: Thiamine is vital for the development of cochlear hair cells during both prenatal and postnatal periods. Even partial deficiency of thiamine causes significant degeneration to the auditory pathway. Level of evidence: The level of evidence of this article is 5. This article is an experimental animal and laboratory study.Publication Metadata only The ameliorative effects of melatonin on acetic acid-induced gastric ulcer in rats via its modulatory effects on gut microbiota(CUKUROVA UNIV, FAC MEDICINE, 2021) YILDIRIM, ALPER; Tamer, Sevil Arabaci; Yildirim, Alper; Cevik, Ozge; Aksu, Burak; Yuksel, Meral; Dertsiz, Ekin Kuntsal; Sirvanci, Serap; Yegen, Berrak C.Purpose: The aim of this study was of observe the possible protective effects of melatonin pretreatment on oxidative damage and microbiota alteration due to gastric ulcer in rats. Materials and Methods: Wistar-albino rats were given (n=32) melatonin (4 mg/kg/day), antibiotic mixture (AB; 1g/L ampicillin + 1g/L neomycin + 1g/L metronidazole), melatonin+AB in drinking water for 12 days or tap water for 15 days (control group; n=8). Subsequently, ulcer was induced. All treatments were continued for three days. Gastric tissues were obtained for biochemical and histopathological examinations, and fecal samples from the rectum were stored for bacteriological measurements. Results: MPO and MDA levels were increased in untreated ulcer groups compared to the control group. In addition, the levels of luminol-lucigenin chemiluminescence (CL) and 8-OHdG and TNF-alpha and IL-8 protein expressions were also increased, while TNF-alpha, IL-8, MDA, 8-OHdG, luminol and lucigenin CL levels were significantly decreased in the melatonin-treated ulcer groups. However, melatonin+AB pretreatment increased antioxidant GSH levels and anti-inflammatory IL-10 levels, and suppressed caspase-3 activity and reduced MPO back to control level. Conclusion: We anticipate that melatonin treatment, which is an effective antioxidant and radical scavenger, can accelerate ulcer healing along with antibiotics and increase the variety of bacteria impaired by antibiotics in the colon.Publication Metadata only Effect of Tacrolimus on Peripheral Nerve Regeneration in Allograft Transplantation: A Light and Electron Microscopic Study(2021) KAYA, ÖZLEM TUĞÇE; Cilingir-Kaya, Ozlem Tugce; Sumer, Onur; Sirvanci, Serap; Gurler, Esra Bihter; Akcal, Arzu; Karsidag, SemraOBJECTIVES: Peripheral nerve injuries are common in Europe; however, the treatment techniques may lead to disabilities. This study aimed to evaluate the effect of tacrolimus use on the capacity of the epineural sheath graft to improve its regeneration quality in rat sciatic nerves as a treatment option for nerve injuries. MATERIALS AND METHODS: In the experimental process, 30 male Sprague Dawley were used as recipients and 10 Wistar rats were used as donors. Under anesthesia, all rats were operated on to resect the sciatic nerve. The nerve tissue of Wistar rats was used as allograft. In the autograft group, the resected nerve was reversed and sutured, resulting in an epineural sheath graft. For the allograft groups, rats were randomly divided into 2 groups as the tacrolimus-treated group and the nontreated group after allograft transplant. Tacrolimus was administered intramuscularly at 0.1 mg/kg daily for 12 weeks. After the treatment period, rats were killed and evaluated histomorphologically with light and electron microscopy. RESULTS: Histological examination showed no remarkable differences between different regions of the sciatic nerves (distal, middle, and proximal). The axonal density was decreased in the allograft groups compared with the autograft group (P < .001). Results showed that the number of mast cells was increased in the allograft group without tacrolimus treatment (P < .05). Similarly, there was a mild increase in mast cell count in the tacrolimus-treated allograft group. CONCLUSIONS: Our results showed that tacrolimus use in rats with implanted epineural nerve sheath supported recovery in terms of morphological and physiological regeneration of the nerve.Publication Metadata only İntrakardiyak perfüzyon(Ankara Nobel Tıp kitabevleri, 2021-01-01) ŞİRVANCI, SERAP; ŞİRVANCI S.Publication Metadata only alpha 7 nicotinic receptor agonist and positive allosteric modulators differently improved schizophrenia-like cognitive deficits in male rats(ELSEVIER, 2021) ARICIOĞLU, FEYZA; Unal, Gokhan; Sirvanci, Serap; Aricioglu, FeyzaThe majority of schizophrenia patients have cognitive deficits as a separate symptom cluster independent of positive or negative symptoms. Current medicines, unfortunately, cannot provide clear benefits for cognitive symptoms in patients. Recent findings showed decreased alpha 7 nicotinic acetylcholine receptor (nAChR) expressions in subjects with schizophrenia. alpha 7 nAChR full/partial agonists and positive allosteric modulators (PAMs) may be valuable drug candidates to treat cognitive deficits of disease. This study comparatively investigated the effect of alpha 7 nAChR agonist (A-582941), type I PAM (CCMI), type II PAM (PNU-120596), and the antipsychotic drug (clozapine) on behavioral, molecular, and immunohistochemical parameters in a subchronic MK-801 model of schizophrenia in male rats. Novel object recognition (NOR) and Morris water maze (MWM) tests were performed to evaluate recognition and spatial memories, respectively. Gene and protein expressions of parvalbumin, glutamic acid decarboxylase-67 (GAD67), and alpha 7 nAChR were examined in the rats' hippocampal tissue. The subchronic MK-801 administration produced cognitive deficits in the NOR and MWM tests. It also decreased the protein and gene expressions of parvalbumin, GAD67, and alpha 7 nAChR in the hippocampus. Clozapine, A-582941, and PNU-120596 but not CCMI increased the parvalbumin and alpha 7 nAChR expressions and provided benefits in recognition memory. Interestingly, clozapine and CCMI restored the MK-801 induced deficits on GAD1 expression and spatial memory while A-582941 and PNU-1 20 596 were ineffective. These results indicated that alpha 7 nAChR agonist, type I and type II PAMs may provide benefits in different types of cognitive deficits rather than a complete treatment in schizophrenia.Publication Open Access NLRP1-Mediated Antidepressant Effect of Ketamine in Chronic Unpredictable Mild Stress Model in Rats(KOREAN NEUROPSYCHIATRIC ASSOC, 2020-04-15) ARICIOĞLU, FEYZA; Aricioglu, Feyza; Yalcinkaya, Canan; Ozkartal, Ceren Sahin; Tuzun, Erdem; Sirvanci, Serap; Kucukali, Cem Ismail; Utkan, TijenObjective NOD-like receptor protein 1 (NLRP1) inflammasome complex has been recently associated with chronic unpredictable mild stress (CUMS) model of depression. Our aim was to investigate whether ketamine-induced antidepressant effect is associated with suppression of NLRP1. Methods Wistar albino rats were divided into control, CUMS, CUMS+acute ketamine (a single 10 mg/kg dose) and CUMS+chronic ketamine (daily 10 mg/kg injections for 3 weeks) groups (n=10 for each group). Sucrose preference test and forced swimming test were performed to assess anhedonia and immobility time respectively for the severety of depression symptoms. Brain tissues were dissected and prefrontal cortex and hippocampus regions were used for real-time polymerase chain reaction (PCR) and immunohistochemical analysis. Results CUMS procedure significantly induced depressive-like symptoms whereas both acute and chronic ketamine treatment ameliorated them. mRNA expression levels of NLRP1, caspase 1, apoptosis-associated speck-like protein containing a CARD (ASC), NF-kappa B, endothelial nitric oxide synthase, IL-1 beta, IL-6, toll-like receptor 4 (TLR-4) and purinergic 2x7 receptor (P2X7R) and numbers of Iba-1+and GFAP+glial cells were reduced by acute and/or chronic ketamine treatment. Conclusion In the present study for the first time upstream and downstream elements of the NLRP1 inflammasome complex are shown to be suppressed by ketamine thus reinforcing the involvement of NLRP1 in the physiopathology of depression.