Person: ŞİRVANCI, SERAP
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ŞİRVANCI
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SERAP
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Publication Open Access The effects of alpha-lipoic acid (ALA) on the urinary bladder injury in rats exposed to chronic stress: a histochemical study(2022-01-01) ŞİRVANCI, SERAP; Yurdakul N., Cikler E., Toklu H. Z. , ŞİRVANCI S.Objective: In the present study, we aimed to investigate the morphological and biochemical effects of alpha-lipoic acid (ALA) on bladder injury caused by water avoidance stress (WAS) and to show its effect on the number of degranulated mast cells, which increase after stress.Materials and Methods: Wistar albino rats were subjected to WAS and the animals in the treatment group were injected ALA. After the urinary bladder tissues were subjected to routine tissue processing, hematoxylin-eosin staining and periodic acid-Schiff reaction were applied to observe general morphology and acidic toluidine blue method to investigate mast cells. Biochemical assessments of malondialdehyde (MDA) and glutathione (GSH) were also obtained. Transmission electron microscope was used for the ultrastructural, and scanning electron microscope for the topographical analyses. Results: The experiments showed that chronic stress caused injury in the bladder, increased degranulated and total number of mast cells and decreased GSH and increased MDA levels. ALA treatment after WAS ameliorated bladder injury in most areas, decreased degranulated and total mast cell number and increased GSH and decreased MDA levels.Conclusion: It was concluded that ALA can be a useful agent in the treatment of interstitial cystitis.Publication Open Access Testicular apoptosis and proliferation in relation to body mass index and m-TESE success in nonobstructive azoospermic men(2023-04-01) ŞİRVANCI, SERAP; Kuntsal Dertsiz E., Kadıoğlu A., Şirvancı S.Male infertility occurs due to the disruption of the balance of proliferation and apoptosis in the testicular tissue. It is important toshow the effect of increased body mass index, which is one of the factors that disrupts this balance of apoptosis and proliferation,not only at the blood hormone level but also at the tissue level. For this reason, the present study is aimed at demonstrating therelation between body mass index and cell turnover in the testis using immunohistochemical methods. In the present study,patients were grouped as normal, overweight and obese, and as m-TESE positive and negative. The sperm retrieval rate withmicrosurgical testicular sperm extraction (m-TESE) was 41.67%. Histological diagnosis of testicular tissues was made withhematoxylin and eosin and Masson’s trichrome staining. Apoptosis and proliferation in the testicular tissue were demonstratedby TUNEL and PCNA immunohistochemical methods, respectively. It was concluded that BMI had no significant effect onreproductive hormone profile (FSH, LH, and testosterone), m-TESE success, apoptosis, and proliferation in testicular tissue innonobstructive azoospermic men. In addition TUNEL positivity and proliferative index was found to be significantly correlatedwith testicular histology and m-TESE outcome.Publication Open Access Investigation of neurogenesis in kindled wistar and genetic absence epilepsy rats(2022-09-01) ŞİRVANCI, SERAP; KAYA, ÖZLEM TUĞÇE; Kandemir C., Yavuz M., Karakaya F. B. , Kaya Ö. T. , Onat F., Şirvancı S.Publication Open Access Effect of perinatal and postnatal thiamine deficiency on auditory pathway of the Wistar-Albino rats(2022-01-01) KAYA, ÖZLEM TUĞÇE; ŞİRVANCI, SERAP; Gür Ö. E., Yılmaz N. D. S., Ensari N., Senirli R. T., GÜLMEZ Z. D., KAYA Ö. T., ŞİRVANCI S., Danışman B., DERİN N., Yılmaz M. D.© 2022 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-FacialObjective: In this study, we created an animal model to demonstrate the effects of thiamine on the hearing pathways of new-borns during pregnancy and lactation by inducing a dietary thiamine deficiency in the mother. Methods: The study included 16 female Wistar albino rats. The animals were separated into four groups and provided the appropriate amounts of dietary thiamine according to their groups during pre-pregnancy, pregnancy, and lactation periods. Three pups from each mother were included in the study, and 12 pups were selected from each group. On the fortieth day after birth, the auditory pathways of 48 pups in the 4 groups were examined electro physiologically and ultra-structurally. Results: In Group N-N, morphology of hair cells stereocilia degeneration was not obtained in all turns of cochlea. In Group N-T, Inner Hair Cells (IHCs) and Outher Hair Cells (OHCs) stereocilia didn\"t show degeneration in all turns of cochlea but had rupture inrows of HCs stereocilia. In group T-N IHCs stereocilia less degeneration was observed in all turns of cochlea. OHC stereocilia partial loss was observed only in basal turn of cochlea. In Group T-T IHCs stereocilia was observed less degeneration and rupture in all turns of cochlea. Conclusion: Thiamine is vital for the development of cochlear hair cells during both prenatal and postnatal periods. Even partial deficiency of thiamine causes significant degeneration to the auditory pathway. Level of evidence: The level of evidence of this article is 5. This article is an experimental animal and laboratory study.Publication Open Access Antidepressant-like Effects Induced by Chronic Blockade of the Purinergic 2X7 Receptor through Inhibition of Non-like Receptor Protein 1 Inflammasome in Chronic Unpredictable Mild Stress Model of Depression in Rats(KOREAN COLL NEUROPSYCHOPHARMACOLOGY, 2019-05-31) ARICIOĞLU, FEYZA; Aricioglu, Feyza; Ozkartal, Ceren Sahin; Bastaskin, Tugce; Tuzun, Erdem; Kandemir, Cansu; Sirvanci, Serap; Kucukali, Cem Ismail; Utkan, TijenObjective: Purinergic 2X7 receptor (P2X7R) activation is known to be involved in pathogenesis of depression. Our aims were to investigate P2X7R-activated inflammasome pathways in parallel with induction of depression and to test the antidepressant-like effects of the selective P2X7R antagonist Brilliant Blue G (BBG) in a rat model of chronic unpredictable mild stress (CUMS). Methods: Male Wistar albino rats were divided into control, CUMS, CUMS+BBG25 (25 mg/kg/day) and CUMS+BBG50 (50 mg/kg/day) groups (n=10 for each group). Various stressors were applied to rats for 6 weeks to establish the CUMS model and daily BBG treatment was started at the end of 3rd week. Sucrose preference test and forced swim test (FST) were performed to assess antidepressant-like effects. Brain samples were obtained for real-time polymerase chain reaction and immunohistochemistry analysis. Results: In FST, duration of immobility was reduced in the CUMS+BBG50 group. Also, BBG treatment significantly enhanced sucrose preference. While NLRP3 gene expression levels were unchanged in rats exposed to the CUMS protocol, expression levels of other inflammasome pathway factors NLRP1, caspase-1, ASC, NF-kappa B, IL-1 beta, IL-6 and P2X7R were increased. BBG treatment reduced expression levels of these factors. Likewise, Iba-1 and GFAP immunoreactivities were enhanced by the CUMS protocol and this action was reversed by BBG treatment. Conclusion: Chronic administration of BBG in CUMS model results in antidepressant-like activity in a dose dependent manner. Molecular and histological results show that these effects might be at least partially related to the suppression of inflammasome-related neuroinflammatory responses and suggest involvement of NLRP1 in depression.Publication Open Access NLRP1-Mediated Antidepressant Effect of Ketamine in Chronic Unpredictable Mild Stress Model in Rats(KOREAN NEUROPSYCHIATRIC ASSOC, 2020-04-15) ARICIOĞLU, FEYZA; Aricioglu, Feyza; Yalcinkaya, Canan; Ozkartal, Ceren Sahin; Tuzun, Erdem; Sirvanci, Serap; Kucukali, Cem Ismail; Utkan, TijenObjective NOD-like receptor protein 1 (NLRP1) inflammasome complex has been recently associated with chronic unpredictable mild stress (CUMS) model of depression. Our aim was to investigate whether ketamine-induced antidepressant effect is associated with suppression of NLRP1. Methods Wistar albino rats were divided into control, CUMS, CUMS+acute ketamine (a single 10 mg/kg dose) and CUMS+chronic ketamine (daily 10 mg/kg injections for 3 weeks) groups (n=10 for each group). Sucrose preference test and forced swimming test were performed to assess anhedonia and immobility time respectively for the severety of depression symptoms. Brain tissues were dissected and prefrontal cortex and hippocampus regions were used for real-time polymerase chain reaction (PCR) and immunohistochemical analysis. Results CUMS procedure significantly induced depressive-like symptoms whereas both acute and chronic ketamine treatment ameliorated them. mRNA expression levels of NLRP1, caspase 1, apoptosis-associated speck-like protein containing a CARD (ASC), NF-kappa B, endothelial nitric oxide synthase, IL-1 beta, IL-6, toll-like receptor 4 (TLR-4) and purinergic 2x7 receptor (P2X7R) and numbers of Iba-1+and GFAP+glial cells were reduced by acute and/or chronic ketamine treatment. Conclusion In the present study for the first time upstream and downstream elements of the NLRP1 inflammasome complex are shown to be suppressed by ketamine thus reinforcing the involvement of NLRP1 in the physiopathology of depression.Publication Open Access Comparison of numbers of interneurons in three thalamic nuclei of normal and epileptic rats(SPRINGER, 2014-06) AKAKIN, DİLEK; Cavdar, Safiye; Bay, Husniye Hacioglu; Yildiz, Sercan D.; Akakin, Dilek; Sirvanci, Serap; Onat, FilizThe inhibitory sources in the thalamic nuclei are local interneurons and neurons of the thalamic reticular nucleus. Studies of models of absence epilepsy have shown that the seizures are associated with an excess of inhibitory neurotransmission in the thalamus. In the present study, we used light-microscopic gamma-aminobutyric acid (GABA) immunocytochemistry to quantify the interneurons in the lateral geniculate (LGN), ventral posteromedial (VPM), and ventral posterolateral (VPL) thalamic nuclei, and compared the values from normal Wistar rats and genetic absence epilepsy rats from Strasbourg (GAERS). We found that in both Wistar rats and GAERS, the proportion of interneurons was significantly higher in the LGN than in the VPM and VPL. In the LGN of Wistar rats, 16.4% of the neurons were interneurons and in the GAERS, the value was 15.1%. In the VPM, the proportion of interneurons was 4.2% in Wistar and 14.9% in GAERS; in the VPL the values were 3.7% for Wistar and 11.1% for the GAERS. There was no significant difference between Wistar rats and the GAERS regarding the counts of interneurons in the LGN, whereas the VPM and VPL showed significantly higher counts in GAERS. Comparison of the mean areas of both relay cells and interneuronal profiles showed no significant differences between Wistar rats and GAERS. These findings show that in the VPL and the VPM there are relatively more GABAergic interneurons in GAERS than in Wistar rats. This may represent a compensatory response of the thalamocortical circuitry to the absence seizures or may be related to the production of absence seizures.Publication Open Access Loop nerve graft prefabrication for peripheral nerve defect reconstruction(2022-08-01) ŞİRVANCI, SERAP; Oksuz S., Eren F., Cesur C., AÇIKEL ELMAS M., ŞİRVANCI S.BACKGROUND: Delayed autologous nerve graft reconstruction is inevitable in devastating injuries. Delayed or prolonged repair time has deleterious effects on nerve grafts. We aimed improving and accelerating nerve graft reconstruction process in a rat long nerve defect model with loop nerve graft prefabrication particularly to utilize for injuries with tissue loss. METHODS: Twenty-four Sprague-Dawley rats were allocated into three groups. 1.5 cm long peroneal nerve segment was excised, reversed in orientation, and used as autologous nerve graft. In conventional interpositional nerve graft group (Group 1), nerve defects were repaired in single-stage. In loop nerve graft prefabrication group (Group 2), grafts were sutured end-to-end (ETE) to the proximal peroneal nerve stumps. Distal ends of the grafts were sutured end-to-side to the peroneal nerve stumps 5 mm proximal to the ETE repair sites in first stage. In second stage, distal ends of the prefabricated grafts were transposed and sutured to distal nerve stumps. In staged conventional interpositional nerve graft group (Group 3), grafts were sutured ETE to proximal peroneal nerve stumps in first stage. Distal ends of the grafts and nerve stumps were tacked to the surrounding muscles until the final repair in second stage. Followup period was 4 weeks for each stage in Groups 2 and 3, and 8 weeks for Group 1. Peroneal function index (PFI), electrophysiology, and histological assessments were conducted after 8 weeks. P<0.05 was considered significant for statistical analysis. RESULTS: PFI results of Group 1 (−22.75±5.76) and 2 (−22.08±6) did not show statistical difference (p>0.05). Group 3 (−33.64±6.4) had a statistical difference compared to other groups (p<0.05). Electrophysiology results of Group 1 (16.19±2.15 mV/1.16±0.21 ms) and 2 (15.95±2.82 mV/1.17±0.16 ms) did not present statistical difference (p>0.05), whereas both groups had a statistical difference compared to Group 3 (10.44±1.96 mV/1.51±0.15 ms) (p<0.05). Axon counts of Group 1 (2227±260.4) and 3 (2194±201.1) did not have statistical difference (p>0.05), whereas both groups had significantly poor axon counts compared to Group 2 (2531±91.18) (p<0.05). CONCLUSION: Loop nerve graft prefabrication improved axonal regeneration without delay. Loop prefabrication can accelerate prolonged regeneration time for the injuries indicating a delayed nerve reconstruction. Higher axon counts derived with loop nerve prefabrication may even foster its investigation in immediate long nerve defect reconstructions in further studies.Publication Open Access Anti-inflammatory effects of montelukast on smoke-induced lung injury in rats(NOVAMEDIA, 2010) ERCAN, FERİHA; Basyigit, Ilknur; Sahin, Murat; Sahin, Deniz; Yildiz, Fusun; Boyaci, Hasim; Sirvanci, Serap; Ercan, FerihaAim: To evaluate the effects of montelukast in smoke-induced lung injury. Methods: 28 Wistar-Albino rats were enrolled into 4 groups with 7 rats per group. The healthy control group was exposed to fresh air while all rats in the 3 experimental groups were exposed to cigarette smoke for 20 weeks for 2 hours per day. After histopathological verification of smoke induced lung injury, montelukast (0.1 mg/kg) dissolved in Na(2)CO(3) was given in one group (MON), Na(2)CO(3) only was given in another group (MON control) and placebo was injected in the third group (COPD control) intraperitoneally for 21 days. At the end of this period blood samples were obtained for serum TNF-alpha assessment and light and electron microscopy analyses were performed on the lung tissues of sacrificed rats. Results: Serum TNF-a levels in the MON group were significantly lower than in the MON control and COPD control groups (38.84 +/- 4.9 pg/ml, 77.5 +/- 5.8 pg/ml and 79.2 +/- 6.9 pg/ml respectively, p < 0.05). Furthermore there was no statistically significant difference between the MON group and healthy controls with respect to serum TNF-alpha levels (38.84 +/- 4.9 pg/ml vs. 29.5 +/- 3.6 pg/ml, p > 0.05). Light and electron microscopic evaluation of the lungs demonstrated that the total histopathological damage score of the lung samples was significantly lower in the MON group than in MON controls and COPD controls (5.14 +/- 0.5, 8.4 +/- 0.6 and 8.7 +/- 0.4 respectively, p < 0.05), while there was no significant difference between the MON group and healthy controls (5.1 +/- 0.6 vs 2.3 +/- 0.2, p > 0.05). Conclusion: These findings suggest that montelukast might have a protective effect on smoke-induced lung injury in rats both from a histopathological and inflammatory point of view.Publication Open Access Evaluation of the Effect of Betahistine on Noise-Induced Hearing Loss Using Distortion Product Otoacoustic Emission and Scanning Electron Microscopy(AVES, 2015-07-07) ŞİRVANCI, SERAP; Yilmaz, Hilmi; Aydin, Sedat; Sanli, Arif; Erdogan, Banu Atalay; Kibar, Sermin; Sirvanci, Serap; Kiran, DemirOBJECTIVE: To evaluate the protective effect of betahistine on noise-induced hearing loss (NIHL) using scanning electron microscopy (SEM) and distortion product otoacoustic emission (DPOAE). MATERIALS and METHODS: A total of 8 adult albino guinea pigs were used in this study (study group: n= 4 and control group: n= 4). DPOAE measurements of both groups were performed before the procedure. Two hours before noise exposure, 0.9% NaCl solution was injected perorally to the control group and betahistine was administered through a peroral catheter to the study group. Both groups were then exposed to 105-dB sound pressure level (SPL) 4-kHz frequency-based narrow-band noise for 2 h per day for 5 days. DPOAE measurements were performed again on the 6th day and cochleae were dissected and examined by SEM on the 7th day. RESULTS: Regarding the results of DPOAE, NIHL was observed in both groups on the 6th day (p< 0.05). Loss, flattening, and fusion, which are findings of permanent hearing loss, were determined in the stereocilia of the inner and outer hair cells by SEM. These findings were evaluated as signs of permanent increase in the threshold. When DPOAE measurements and SEM results were evaluated in the study group, no significant difference was observed in NIHL compared with the control group (p> 0.05). CONCLUSION: In our study, it was observed that simultaneous administration of betahistine during noise had no protective effect on permanent increase in the threshold. However, further studies on noise and long-term use of betahistine can be performed.