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TINAZ, GÜLGÜN

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Author: CESUR, SÜMEYYE ×

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    PublicationOpen Access
    The Role of Multilayer Electrospun Poly(Vinyl Alcohol)/Gelatin nanofibers loaded with Fluconazole and Cinnamaldehyde in the Potential Treatment of Fungal Keratitis
    (2022-08-01) GÜNDÜZ, OĞUZHAN; TINAZ, GÜLGÜN; ŞENGÖR, MUSTAFA; CESUR, SÜMEYYE; Ilhan E., Cesur S., Sulutas R. B., Pilavci E., Dalbayrak B., Kaya E., Arisan E. D., Tinaz G., ŞENGÖR M., Kijeenska-Gawaronska E., et al.
    Fungal keratitis is a severe corneal infection that causes irreversible damage to the cornea, for which conventional drug treatments may be insufficient. With the new generation of drug delivery systems, it is desired to ensure the ocular usability of drugs. In this study, two-layer polyvinyl alcohol and gelatin (PVA/GEL) nanofibers with high drug loading capacity were produced by the electrospinning method. Cinnamaldehyde (CA), an FDAapproved volatile molecule found in cinnamon essential oil, and fluconazole (FLU), an antifungal drug, were incorporated into PVA/GEL nanofibers to inhibit the growth and biofilm formation of Candida albicans, one of the pathogens that cause fungal keratitis. The morphology, chemical structures, and thermal transitions of the produced pure (PVA/GEL), FLU loaded (PVA/GEL/FLU), CA loaded (PVA/GEL/CA), and combined FLU and CA loaded (PVA/GEL/COM) nanofibers were analysed by scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC), respectively. The mechanical analysis, swelling and degradation behaviour, and drug release kinetics of the nanofibers were investigated. PVA/ GEL/FLU, PVA/GEL/CA and PVA/GEL/FLU/COM nanofibers were evaluated for their antifungal and antibiofilm activity against Candida albicans. Results showed that PVA/GEL/FLU and PVA/GEL/COM nanofibers have significant antifungal activity and inhibited biofilm formation by 37% and 49%, respectively. Furthermore, it was determined by MTT analysis using a human embryonic kidney (HEK) that the nanofibers were not cytotoxic. In the treatment of fungal keratitis, double-layer PVA/GEL/COM nanofiber with CA in the first layer and FLU in the second layer can create a new treatment approach as an alternative drug delivery system.
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    PublicationMetadata only
    Multilayer patches for the treatment of corneal perforations caused by infections
    (2022-09-28) CESUR, SÜMEYYE; TINAZ, GÜLGÜN; ŞENGÖR, MUSTAFA; OKTAR, FAİK NÜZHET; GÜNDÜZ, OĞUZHAN; Kijeńska-Gawrońska ., İlhan E., Cesur S., Sulutaş R. B., Pilavcı E., Dalbayrak B., Kaya E., Arısan E. D., Tınaz G., Şengör M., et al.
  • Thumbnail Image
    PublicationOpen Access
    A novel strategy as a potential rapid therapy modality in the treatment of corneal ulcers: Fluconazole/vancomycin dual drug-loaded nanofibrous patches
    (2023-01-01) CESUR, SÜMEYYE; BİNGÖL ÖZAKPINAR, ÖZLEM; TINAZ, GÜLGÜN; OKTAR, FAİK NÜZHET; GÜNDÜZ, OĞUZHAN; CESUR S., Ilhan E., Pilavci E., Sulutas R. B., Gurboga M., BİNGÖL ÖZAKPINAR Ö., Kaya E., Heljak M., TINAZ G., OKTAR F. N., et al.
    Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual-drug loaded dual-layered polyvinyl alcohol and gelatin (PVA/GEL) nanofibrous patches are produced by electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces are obtained for both pure and drug-loaded nanofibrous patches. The tensile test results report that loading the FLU and VAN separately into the PVA/GEL patches decrease both the tensile strength and elongation at break and it is further reduced when combining two drug-loaded layers in one patch. According to drug release results, the FLU and VAN-loaded nanofibrous patches show a controlled release profile extending up to 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, and PVA/GEL/FLU/VAN nanofibrous patches display significant antimicrobial activity against Candida albicans and Staphylococcus aureus. SEM, 4\"-6diamidynofenyloindol (DAPI) staining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay show that PVA/FLU and PVA/GEL/FLU/VAN nanofibrous patches have a superior effect on NIH3T3 cell spreading and proliferation. The novelty of this study lays in the development of a potential dual drug rapid treatment for corneal ulcers of aggressive nature.