Person: RAYAMAN, ERKAN
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RAYAMAN
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ERKAN
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Publication Metadata only The biological activities of endemic Ballota pseudodictamnus subsp. lycia(2022-05-25) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; TAŞKIN, TURGUT; Akduman B., RAYAMAN E., YILMAZ B. N., Ermanoğlu M., GÜRDAL ABAMOR M. B., TAŞKIN T.Publication Metadata only Probiyotik ürünlerden izole edilen laktik asit bakterilerinin ultrasonikasyon sonrası antimikrobiyal etkinliğinin belirlenmesi(2023-08-15) RAYAMAN, PERVİN; RAYAMAN, ERKAN; BİNGÖL ÖZAKPINAR, ÖZLEM; Bolat G., RAYAMAN P., RAYAMAN E., BİNGÖL ÖZAKPINAR Ö.Giriş ve Amaç: Yeterli miktarlarda alındığında intestinal sistemi düzenleyerek konakçı sağlığı üzerine faydalı etkilere sahip canlı mikroorganizma suşları olarak tanımlanan probiyotiklerin bağırsak epiteliyal bariyer fonksiyonunu iyileştirdiği, immünomodülatör yanıtları düzenlediği, toksin reseptörlerini bozduğu, inhibitör maddeler ürettiği, çeşitli mekanizmalar kullanarak patojen mikroorganizmaları inhibe ettiği bilinmektedir. Dolayısıyla çalışmamızda, çeşitli probiyotik ürünlerden izole edilen laktik asit bakterilerinin ultrasonikasyon sonrası antimikrobiyal etkinliğinin belirlenmesi hedeflenmiştir. Gereç ve Yöntem: Çalışmamızda; Lactobacillus fermentum ATCC (Amerikan Tipi Kültür Koleksiyonu) 14931, ev yapımı kefirden izole edilen Lactobacillus kefiri ve eczane preparatından izole edilen Lactobacillus rhamnosus türü mikroorganizmaların in vitro koşullarda Escherichia coli ATCC 11229, Staphylococcus aureus ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Salmonella typhimurium ATCC 14028, Klebsiella pneumoniae ATCC 4352 bakterileri ve Candida albicans ATCC 10231 mayası üzerine olan antimikrobiyal aktivitesi spot on lawn yöntemiyle belirlenmiştir. Çalışmamızda bulunan mikroorganizmalar Marmara Üniversitesi Farmasötik Mikrobiyoloji Anabilim Dalı’nın kültür stok koleksiyonundan temin edilmiştir. Çalışmamızdaki Laktobacillus türleri 15 ml’lik Falcon tüplerine aktarılmış ve MRSA (Mann Rogosa Sharpe Agar) içeren petrilere damlatılarak inkübe edilmiştir. İnkübasyon sonrası probiyotik mikroorganizmalara ultrason cihazında ms 73 prob, %50 genişlik ayarlanarak; 6 dakika boyunca ultrasonik homojenikatör uygulanmış ve MRSA besiyeri içeren petrilerin yüzeyine 5 μL damlatılarak inkübe edilmiştir. İnkübasyon sonrası probiyotik mikroorganizmaların bulunduğu petri plaklarının üzerine McFarland 0,5 standart bulanıklığına ayarlanmış bakteriler ve McFarland 1 standart bulanıklığına ayarlanmış maya süspansiyonu içeren yumuşak agar dökülmüş ve petriler bakteriler için 24 saat, maya için ise 48 saat süre ile 37°C’de inkübe edildikten sonra oluşan inhibisyon zonlarının çapı elektronik kumpas yardımı ile mm olarak ölçülüp kaydedilmiştir. Bulgular ve Sonuç: Çalışmada yer alan tüm probiyotik mikroorganizmalar ultrasonikasyon sonrası Escherichia coli ATCC 11229, Staphylococcus aureus ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 4352 patojenlerine karşı başlangıç değerine göre daha fazla antimikrobiyal etki göstermiştir. Lactobacillus fermentum ve Lactobacillus kefiri türlerinin Candida albicans’a karşı başlangıçta etki edemezken ultrasonikasyon sonrası inhibisyon zonu oluşturduğu tespit edilmiştir. Sonuç olarak ultrasonikasyon işleminin probiyotik ürünlerden izole edilen laktik asit bakterilerinin antimikrobiyal etkinliğini arttırdığı gösterilmiştir. Çalışmamızın bu alanda yapılacak araştırmalara ışık tutacağı kanısındayız.Publication Metadata only Investigation on biological activities of Melissa officinalis subsp. altissima(2022-05-25) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., RAYAMAN E., ÖZDATLI KURTULUŞ Ş., Ermanoğlu M., YILMAZ B. N., ŞENKARDEŞ İ., TAŞKIN T.Publication Metadata only Investigation of antioxidant and antibicrobial activities of muscari armeniacum(2023-05-04) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; EMRE, GİZEM; TAŞKIN, TURGUT; Avcı E., Rayaman E., Yılmaz B. N., Emre G., Taşkın T.Publication Open Access Investigation on biological activities of Melissa officinalis subsp. altissima(2022-05-23) RAYAMAN, ERKAN; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., Rayaman E., Özdatlı Kurtuluş Ş., Ermanoğlu M., Yılmaz B. N. , Şenkardeş İ., Taşkın T.The genus Melissa is used as antibacterial, sedative, digestive aid, carminative, diaphoretic and bile secretion enhancer. It is included in the composition of the preparations used during nervous digestive system disorders, psychosomatic heart disorders and migraine. The flowering branches and leaves of Melissa officinalis L. subsp. altissima. are used as carminative, sedative and its antipyretic effects. It is also used in vascular occlusion, stomach aches, heart ailments, kidney inflammation, cholesterol, blood pressure, and diabetes. According to our knowledge there are not enough studies on these species grown in Turkey. The aim of this study is to examine the antioxidant, cytotoxic, anticholinesterase, anti-urease and antimicrobial activities of plant’ various extracts obtained by using the sequential maceration method. In the determination of antimicrobial activity well diffusion method on 6 gram negative, 4 gram positive and 3 yeast strains was used. DPPH, CUPRAC and FRAP techniques were used to examine the antioxidant properties of plant\" extracts. The extracts\" anticholinesterase, cytotoxic and anti-urease activity were determined using the Ellman, MTT and Indophenol techniques, respectively. In addition, the phenolic contents of the extracts were determined by FCR method. It was found that petroleum ether extract of the plant was effective against Enterecoccus faecalis and Staphylococcus aureus strains and ethyl acetate extract were effective against Staphylococcus aureus strains. The methanol extract of the plant exhibited the highest antioxidant activity when compared to other extracts. It was determined that the petroleum ether extract of Melissa officinalis L. subsp. altissima has stronger acetylcholinesterase and urease enzyme inhibition potential than its other extracts. Furthermore, in this study the ethyl acetate extract of the plant was found to have high cytotoxic activity.Publication Metadata only Probiyotik tablet formülasyonlarında kullanılan yardımcı maddelerin bakteri sağ kalımına etkisinin değerlendirilmesi(2023-03-19) ŞAHBAZ, SEVİNÇ; RAYAMAN, ERKAN; UĞURLU, TİMUÇİN; Kandur B., Şahbaz S., Rayaman E., Uğurlu T.Publication Open Access Antimicrobial effect of probiotic microorganisms onclinical and standard Staphylococcus aureus isolates(2023-12-01) RAYAMAN, PERVİN; RAYAMAN, ERKAN; TETİK N., RAYAMAN P., RAYAMAN E., ADALETİ R.In this study we aimed to identify probiotic microorganisms in various pharmacy preparations, market and homemade probiotic products and investigate the antimicrobial effect of these microorganisms on clinical and standard Staphylococcus aureus isolates. The probiotic microorganisms were isolated from probiotic products mentioned above, then identified by Matrix Assisted Laser Ionization Mass Spectrophotometer (MALDI-TOF, Biomeriux) and also by conventional methods. The tolerance of these probiotic microorganisms to different salt, pH and temperature conditions was also detected. The antimicrobial activity of the specified probiotic microorganisms on S. aureus was designated by using spot on lawn and agar well diffusion methods. In our study among 16 probiotic products, 27 various probiotic microorganisms were specified by MALDI-TOF. Additionally, 15 of these probiotic microorganism species had an isolated reliability value above 90%. In our study 10 probiotic microorganism species, 7 of which were different from each other were found to maintain their viability in three various pH, salt and temperature conditions. These probiotic microorganisms were Lactobacillus rhamnosus (P.2 probiotic pharmaceutical product), Enterococcus gallinarum (homemade whey), Lactobacillus kefiri 1 (homemade kefir), Bacillus megaterium (homemade pickled juice), Lactobacillus kefiri 2 (homemade kefir), Lactobacillus rhamnosus (P.1 company preparation) Lactobacillus plantarum (P.3 company preparation), Bifidobacterium spp (M.4 company pure kefir), Enterococcus faecium (P.3 company preparation). We determined that Lactobacillus plantarum isolated from homemade whey had the highest antimicrobial activity on clinical MRSA 3 (Methicillin resistant Staphylococcus aureus) strain (inhibition zone =45.62 mm ± 0.84. Also, we have found that, probiotic microorganisms isolated from pharmacy preparations, market and homemade probiotic products showed antibacterial effect on clinical and standard Staphylococcus aureus by spot on lawn method. We designated that besides probiotic microorganisms isolated from various pharmacy preparations, probiotic microorganisms isolated from market and homemade products were efficient against clinical and standard S. aureus isolatesPublication Open Access The Biological Activities of Endemic Ballota pseudodictamnus subsp. lycia.(2022-05-23) RAYAMAN, ERKAN; TAŞKIN, TURGUT; Akduman B., Rayaman E., Yılmaz B., Ermanoğlu M., Gürdal B., Taşkın T.The genus Ballota L. (Lamiaceae) includes species known as boz ot, bal otu, nemnem otu and have medicinal uses among the people. Additionally, it known to be used by the public in the treatment of cough, asthma, headache, nausea, hemorrhoids, wounds and burns. Ballota taxa have various phytochemical classes such as essential oils, iridoids, saponins, terpenoids, flavonoids, phenolic acids, tannins, and organic acids. Ballota pseudodictamnus subsp. lycia is one of the endemic species growing in southwestern Turkey. As a result of our research on the literature a limited number of studies have been found on the biological activities of this species. The aim of this study is to examine the antioxidant, antimicrobial, anticholinesterase and anti-urease activities of different extracts (petroleum ether, chloroform and methanol) obtained from the aerial part of B. pseudodictamnus subsp. lycia. DPPH, CUPRAC, and FRAP techniques were used to examine the antioxidant properties of plant\" extracts. The extracts\" anti-urease, anticholinesterase and antimicrobial activity were determined using the Indophenol, Ellman and agar well diffusion method techniques, respectively. While the petroleum ether extract obtained from plant, was found to be effective against S. aureus, S.epidermidis and E. faecalis strains; its chloroform extract was effective against S.aureus and S.epidermidis strains and its methanol extract was found to be effective against Acinetobacter baumannii strains. It was found that the plant’ methanol extract showed the highest antioxidant activity when compared to the other extracts.Publication Open Access Chemical Composition and In Vitro Biological Activity of Two Thymus L. Varieties Growing in Turkey(2024-01-01) TAŞKIN, TURGUT; RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; TAŞKIN T., Öksüz M., Bulkurcuoğlu B., Ercelen S., RAYAMAN E., Ermanoğlu M., YILMAZ B. N., Taşkın D., Şahin T., Kılıç Ö.Thymus kotschyanus var. kotschyanus (TKK) and T. kotschyanus var. glabrescens (TKG) varieties were used as both spice and medicine by the people in Turkey. It was determined that plants’ methanol extracts had the strongest antioxidant, anticholinesterase, antiurease activity and high total phenolic contents. The ethyl acetate and methanol extracts were found to have strong antimicrobial activity. Methanol extracts showed low hemolytic effect against human erythrocytes. It was determined that TKG extract showed higher anti-proliferative effect compared to TKK extract. Both plants extract significantly decreased reactive oxygen species (ROS) generation in cancer cells. It was determined that amounts of chlorogenic and rosmarinic acid compounds were similar in both plants, but apigenin 7-O-neohesperidoside compound was found in higher amounts in TKK. The findings obtained in this study suggest that methanol and ethyl acetate extracts obtained from these two species can be used as antioxidant, anticholinesterase, antimicrobial and antiurease agents. The findings support the traditional use of these species.Publication Metadata only Dual-drug delivery of Ag-chitosan nanoparticles and phenytoin via core-shell PVA/PCL electrospun nanofibers(ELSEVIER SCI LTD, 2021) ŞAHİN, ALİ; Hussein, Mohamed Ahmed Mohamady; Guler, Ece; Rayaman, Erkan; Cam, Muhammet Emin; Sahin, Ali; Grinholc, Mariusz; Mansuroglu, Demet Sezgin; Sahin, Yesim Muge; Gunduz, Oguzhan; Muhammed, Mamoun; El-Sherbiny, Ibrahim M.; Megahed, MosaadDual-drug delivery systems were constructed through coaxial techniques, which were convenient for the model drugs used the present work. This study aimed to fabricate core-shell electrospun nanofibrous membranes displaying simultaneous cell proliferation and antibacterial activity. For that purpose, phenytoin (Ph), a well-known proliferative agent, was loaded into a polycaprolactone (PCL) shell membrane, and as-prepared silver-chitosan nanoparticles (Ag-CS NPs), as biocidal agents, were embedded in a polyvinyl alcohol (PVA) core layer. The morphology, chemical composition, mechanical and thermal properties of the nanofibrous membranes were characterized by FESEM/STEM, FTIR and DSC. The coaxial PVA-Ag CS NPs/PCL-Ph nanofibers (NFs) showed more controlled Ph release than PVA/PCL-Ph NFs. There was notable improvement in the morphology, thermal, mechanical, antibacterial properties and cytobiocompatibility of the fibers upon incorporation of Ph and Ag-CS NPs. The proposed core-shell PVA/PCL NFs represent promising scaffolds for tissue regeneration and wound healing by the effective dual delivery of phenytoin and Ag-CS NPs.