Person: AK, ESİN
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AK
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ESİN
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Publication Open Access Comparison of the protective effect of alpha lipoic acid and quercetin in methotrexate- induced lung damage(2023-03-01) AK, ESİN; OKTAY, NİHAL ŞEHKAR; AK E., MUHAN A., ÇALIŞKAN Ş., OKTAY N. Ş.Objective: The aim of this experiment is to investigate and compare the effects of alpha lipoic acid (ALA) and quercetin (QUE) on methotrexate (MTX)-induced lung injury in rats. Method: Wistar Albino rats were distributed into control, MTX, MTX+ALA and MTX+QUE groups with each consisting of 6 rats. Except control group, MTX administrated to rats as a single dose (20 mg/kg) intraperitoneally (i.p.) on the first day. Saline (0.1 cc/100 gr/day, i.p.) was injected to rats in control and MTX groups for 5 days. In MTX+ALA and MTX+QUE groups, rats had injections of ALA (50 mg/kg/day, i.p.) and QUE (50 mg/kg/day, i.p.) for 5 days. After sacrification on day 6, lung tissues were excised out for histopathologic and biochemical investigation. Results: MTX group showed massive hemorrhage with edema in the interstitium, significant inflammatory cell infiltration, and severe alveolar destruction and vascular congestion. Additionally, significant increases in oxidative stress markers as malondialdehyde and sialic acid and significant decreases in antioxidants as glutathione, superoxide dismutase and catalase were detected at the tissue level in MTX group (p<0.0001, p<0.0001, p<0.0001, p=0.03 and p<0.0001, respectively). Both ALA and QUE treatment led to a prominent improvement in morphologic damage. Moreover, ALA and QUE resulted in the reversal of the alterations seen in the tissue oxidative damage markers and antioxidant activities as well. We could not reveal a significant difference between MTX+ALA and MTX+QUE group in terms of morphologic damage and biochemical markers of oxidative injury (p>0.05). Conclusion: Our study showed the similar protective effect of ALA and QUE in MTX induced lung damage. Further studies are warranted to verify the results of our outcome.Publication Metadata only Neuroprotective effects of mitoquinone and oleandrin on Parkinson's disease model in zebrafish(TAYLOR & FRANCIS LTD, 2020) ALTURFAN, EBRU IŞIK; Unal, Ismail; Caliskan-Ak, Esin; Ustundag, Unsal V.; Ates, Perihan S.; Alturfan, Ahmet A.; Altinoz, Meric A.; Elmaci, Ilhan; Emekli-Alturfan, EbruAim: The aim of this study is to investigate the possible protective effects of mitoquinone and oleandrin on rotenone induced Parkinson's disease in zebrafish. Materials and methods: Adult zebrafish were exposed to rotenone and mitoquinone for 30 days. Biochemical parameters were determined by spectrophotometric method and Parkinson's disease-related gene expressions were determined by reverse transcription polymerase chain reaction method. Measurement of neurotransmitters was performed by liquid chromatography tandem-mass spectrometry instrument. The accumulation of synuclein was demonstrated by immunohistochemical staining. In vitro thiazolyl blue tetrazolium bromide method was applied to determine the mitochondrial function of synaptosomal brain fractions using rotenone as a neurotoxic agent and mitoquinone and oleandrin as neuroprotective agents. Results: Mitoquinone improved the oxidant-antioxidant balance and neurotransmitter levels that were disrupted by rotenone. Mitoquinone also ameliorated the expressions of Parkinson's disease-related gene expressions that were disrupted by rotenone. According to thiazolyl blue tetrazolium bromide assay results, mitoquinone and oleandrin increased mitochondrial function which was decreased due to rotenone exposure. Conclusion: Based on the results of our study, positive effects of mitoquinone were observed in Parkinson's disease model induced by rotenone in zebrafish.Publication Metadata only Kronik periodontitisli ve sağlıklı kişilerin tükürük örneklerinin bazı biyokimyasal parametreler ve sitolojik incelemeler açısından karşılaştırılmasında saklama süresinin belirlenmesi(2009-06-20) ALTURFAN, EBRU IŞIK; YARAT, AYŞEN; AK, ESİN; ALTURFAN E. I., YARAT A., AK E., PİŞİRİCİLER R., KURU B., NOYAN Ü.Publication Metadata only Zebra balığı barsak dokusunda doku faktörü ekspresyonunun incelenmesi(2015-03-08) AK, ESİN; ALTURFAN, EBRU IŞIK; ÜSTÜNDAĞ Ü. V., AK E., ALTURFAN E. I.Publication Metadata only Bisphenol A reveals its obesogenic effects through disrupting glucose tolerance, oxidant-antioxidant balance, and modulating inflammatory cytokines and fibroblast growth factor in zebrafish(2022-01-01) ALTURFAN, EBRU IŞIK; AK, ESİN; Beler M., Cansız D., Ünal İ., Üstündağ Ü. V., Dandin E., Ak E., Alturfan A. A., Emekli-Alturfan E. I.Obesogens affect lipid metabolism, and genetic or epigenetic factors may also contribute to the progression of obesity. Endocrine-disrupting chemicals (EDCs) are the most striking among obesogens. Bisphenol A (BPA) is an estrogenic EDC used in food containers, adhesives, dye powders, and dental fillers. We aimed to elucidate molecular mechanisms of BPA\"s obesogenic effects focusing on obesogenic pathways in the liver including fibroblast growth factor (FGF) and Dnmt3a which is its epigenetic regulator, oxidant-antioxidant status, and inflammatory cytokines. Zebrafish were divided into three groups as control, low-dose BPA (1 mu m BPA), and high-dose BPA groups (10 mu m BPA). At the end of 30 days, oral glucose tolerance test (OGTT) was performed, fasting blood glucose levels were measured, and hepatopancreas tissues were taken. Malondialdehyde (MDA) levels, superoxide dismutase (SOD), glutathione S-transferase (GST), and nitric oxide (NO) activities were examined in the hepatopancreas. Inflammatory cytokines, lepa, fgf21, and dnmt3a expressions were determined by RT-PCR. BPA exposure increased the body weights, il1ss, tnf alpha, il6, lepa, fgf21, and dnmt3a expressions, impaired glucose tolerance, and oxidant-antioxidant status in a dose-dependent manner. Hepatocyte degeneration, lipid vacuolization, and vasocongestion were observed in both BPA-exposed groups. Our study suggests impaired glucose tolerance, oxidant-antioxidant balance, increased inflammatory response, fgf21 expression, and dnmt3a expressions as the possible mechanisms for the BPA-induced obesity model in zebrafish.Publication Open Access Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat's teeth(SPRINGER INTERNATIONAL PUBLISHING AG, 2016-12) YARAT, AYŞEN; Demirel, Sezin; Yalvac, Mehmet Emir; Tapsin, Sidika; Akyuz, Serap; Ak, Esin; Cetinel, Sule; Yarat, Aysen; Sahin, FikrettinTreatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases.Publication Metadata only The Protective Role of Chard (Beta Vulgaris L. Var. Cicla) on Amiodarone Induced Submandibular Gland Injury in Rats(2020-10-01) AK, ESİN; AK E., TÜRKYILMAZ İ. B.Publication Metadata only Fibrin sealant induces periodontal tissue healing after tooth replantation in rats.(2012-05-27) AK, ESİN; ÇETİNEL, ŞULE; DEMİREL S., AKYÜZ S. H., YALVAÇ M. E., AK E., ÇETİNEL Ş.Publication Open Access Investigation of the Effects of Edaravone on Valproic Acid Induced Tissue Damage in Pancreas(MARMARA UNIV, FAC PHARMACY, 2017-06-20) YARAT, AYŞEN; Oktay, Sehkar; Alev-Tuzuner, Burcin; Tunali, Sevim; Ak, Esin; Emekli-Alturfan, Ebru; Tunali-Akbay, Tugba; Koc-Ozturk, Leyla; Cetinel, Sule; Yanardag, Refiye; Yarat, AysenValproic acid (VPA), an effective antiepileptic and anticonvulsant drug, has some toxic side effects due to causing elevated oxidant production. The aim of this study is to investigate the effects of edaravone, a potent free radical scavenger on VPA induced toxicity and tissue damage by biochemical and histological examinations on pancreas. Female Sprague Dawley rats were divided into four groups as follows; control, edaravone, VPA, VPA+edaravon. VPA and edaravone were injected intraperitonally for seven days. Total protein, lipid peroxidation (LPO), sialic acid (SA) and glutathione (GSH) levels and alkaline phosphatase (ALP), tissue factor (TF), superoxide dismutase (SOD), glutathione-S-transferase GST), catalase (CAT), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activities were determined in pancreas homogenates. In VPA given group, LPO and SA levels, and ALP, TF, MPO activities significantly increased and GST, CAT, GPx activities significantly decreased compared to control group. A marked morphological damage was detected in the VPA group. Ameliorative effects of edaravone were observed in SA, TF, CAT, GPx parameters and histological examination in the VPA group. Therefore, edaravone may be effective in moderation and/or reduction of toxic effects of VPA on pancreas.Publication Metadata only Sudan Kaçınma Stresi ile Oluşturulan İnterstisyel Sistit Modelinde Oksitosinin Etkisi(2014-05-30) AK, ESİN; ÇETİNEL, ŞULE; AK E., ÇİKLER E., ŞEHİRLİ A. Ö., ŞENER G., TETİK Ş., ÇETİNEL Ş.