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TAŞKIN, TURGUT

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2018 - 201912020 - 20227
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Author: ŞENKARDEŞ, İSMAİL ×

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    Investigation on biological activity of Thymus longicaulis subsp. longicaulis var.subisophyllus and analysis of its phytochemical content by HPLC-DAD
    (2022-08-03) RAYAMAN, PERVİN; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; YILMAZ E., RAYAMAN P., ÖZDATLI KURTULUŞ Ş., ERMANOĞLU M., TAŞKIN D., ŞENKARDEŞ İ., TAŞKIN T.
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    PublicationOpen Access
    The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum
    (UNIV SAO PAULO, CONJUNTO QUIMICAS, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Gezmis, Tugba; Cam, Muhammet Emin; Taskin, Duygu; Celik, Berna Ozbek; Senkardes, Ismail; Suzgec-Selcuk, Sevda
    In Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones arc endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatmy and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinestemse activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.
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    Investigation on biological activities of Melissa officinalis subsp. altissima
    (2022-05-25) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., RAYAMAN E., ÖZDATLI KURTULUŞ Ş., Ermanoğlu M., YILMAZ B. N., ŞENKARDEŞ İ., TAŞKIN T.
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    PublicationOpen Access
    Investigation on biological activities of Melissa officinalis subsp. altissima
    (2022-05-23) RAYAMAN, ERKAN; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., Rayaman E., Özdatlı Kurtuluş Ş., Ermanoğlu M., Yılmaz B. N. , Şenkardeş İ., Taşkın T.
    The genus Melissa is used as antibacterial, sedative, digestive aid, carminative, diaphoretic and bile secretion enhancer. It is included in the composition of the preparations used during nervous digestive system disorders, psychosomatic heart disorders and migraine. The flowering branches and leaves of Melissa officinalis L. subsp. altissima. are used as carminative, sedative and its antipyretic effects. It is also used in vascular occlusion, stomach aches, heart ailments, kidney inflammation, cholesterol, blood pressure, and diabetes. According to our knowledge there are not enough studies on these species grown in Turkey. The aim of this study is to examine the antioxidant, cytotoxic, anticholinesterase, anti-urease and antimicrobial activities of plant’ various extracts obtained by using the sequential maceration method. In the determination of antimicrobial activity well diffusion method on 6 gram negative, 4 gram positive and 3 yeast strains was used. DPPH, CUPRAC and FRAP techniques were used to examine the antioxidant properties of plant\" extracts. The extracts\" anticholinesterase, cytotoxic and anti-urease activity were determined using the Ellman, MTT and Indophenol techniques, respectively. In addition, the phenolic contents of the extracts were determined by FCR method. It was found that petroleum ether extract of the plant was effective against Enterecoccus faecalis and Staphylococcus aureus strains and ethyl acetate extract were effective against Staphylococcus aureus strains. The methanol extract of the plant exhibited the highest antioxidant activity when compared to other extracts. It was determined that the petroleum ether extract of Melissa officinalis L. subsp. altissima has stronger acetylcholinesterase and urease enzyme inhibition potential than its other extracts. Furthermore, in this study the ethyl acetate extract of the plant was found to have high cytotoxic activity.
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    PublicationOpen Access
    In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.
    (2020-06-29) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Dogan, Murat; Cam, Muhammet E.; Sahin, Talip; Senkardes, Ismail
    Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.
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    Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes
    (MARMARA UNIV, INST HEALTH SCIENCES, 2018) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Yesil Devecioglu, Tugce; Senkardes, Ismail
    Objective: Terfezia claveryi is a dark brown color truffle and has been used extensively in human diet in Turkey. Therefore; it is an important issue to determine their biological activities and toxicity. This study aims to investigate the antioxidant and anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity of each extract. Methods: The antioxidant activities of methanol extracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) and ferric reducing/antioxidant power (FRAP) methods. The amount of total phenolic compounds contained in the extracts was determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease activities of methanol extracts were evaluated by indophenol method. In addition, potential DNA damaging effects of T. claveryi extracts on human lymphocytes were determined by the alkaline comet assay. Results: UM extract exhibited higher DPPH radical scavenging activity than MM extract [the concentration of the substance which provides the inhibition of 50% of the radical (IC50): 0.77 +/- 0.01 mg/mL, 1.17 +/- 0.02 mg/mL, respectively, p<0.0001] however, lower activity than standard (p<0.0001). Both extracts in different concentrations of T. claveryi caused DNA damage (p<0.0001) compared with solvent control DMSO. Also, total comet scores was higher in cells which had been incubated with MM extract (p<0.05). UM extract exhibited higher ferric reducing and anti-urease activity than MM extract but not found statistically different; also activities of both extract were found lower than positive controls of each methods (p<0.0001). Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and showed very low antioxidant activity compared to standards. This preliminary study should be confirmed with further studies and in different cell lines.
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    Phytochemical screening and evaluation of antioxidant, enzyme inhibition, anti-proliferative and calcium oxalate anti-crystallization activities of Micromeria fruticosa spp. brachycalyx and Rhus coriaria
    (ELSEVIER, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Dogan, Murat; Yilmaz, Beyza Nur; Senkardes, Ismail
    Micromeria fruticosa spp. brachycalyx species (Lamiaceae) is used as a spice and flavoring agent. Rhus coriaria (Sumac) is widely used both spice and medicinally. In this study, antioxidant, anti-urease, anticholinesterase, anti-proliferative and calcium oxalate anti-crystallization activities of different extracts from M. fruticosa spp. brachycalyx aerial parts and R. coriaria leaves were investigated. Besides, the phytochemical content of these species was analyzed qualitatively and quantitatively. R. coriaria methanol extract showed stronger antioxidant (DPPH, FRAP, CUPRAC, ARTS) and anticholinesterase (46.85%) activity than M. fruticosa spp. brachycalyx extracts. M. fruticosa spp. brachycalyx methanol extract was found to have the highest anti-urease (11.39%) and anti-proliferative (MCF-7: 53.51%) potential. It was observed that the methanol extracts of both plants had a significant inhibition effect on calcium oxalate crystallization. R. coriaria methanol extract was determined to contain high amounts of total flavonoids (0.177 mgQE/mg extract) and tannins (0.365 mg TAE/mg extract) contents. The extracts from both plants have no cytotoxic effects on the healthy cell (L-929) and have strong biological activities in parallel with traditional use, suggesting that these plants may be used as natural sources in the future.
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    Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial
    (BENTHAM SCIENCE PUBL, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Akkilic, Yeliz; Celik, Berna Ozbek; Senkardes, Ismail; Ozakpinar, Ozlem B.
    Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant's different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and tlavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using mnicrodilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (('UPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicatis strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.