Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Metadata only Evaluation of antioxidant, analgesic, anti-inflammatory and antispasmodic activity and genotoxic effect of micromeria fruticosa subsp brachycalyx in vitro and in vivo(2021-07-22) KABASAKAL, LEVENT; TAŞKIN, TURGUT; AYDEMİR, SEZGİN; BECEREN, AYFER; Çelikkol I., Beceren A., Kabasakal L., Taşkın T., Aydemir S.EVALUATION OF ANTIOXIDANT, ANALGESIC AND ANTIINFLAMMAUTORY ACTIVITY AND GENOTOXIC EFFECT OF MICROMERIA FRUTICOSA SUBSP BRACHYCALYX IN VITRO AND IN VIVO1Celikkol I., 2Beceren A., 3Kabasakal L., 4Taskin T., 5Aydemir S.1Marmara University, Institute of Health Sciences, Department of Pharmaceutical Toxicology Istanbul, Turkey, celikkolisik@gmail.com 2Marmara University, Department of Pharmaceutical Toxicology, Istanbul, Turkey, ayfertozan@hotmail.com 3Marmara University, Department of Pharmacology, Istanbul, Turkey, lkabasakal@gmail.com 4Marmara University, Department of Pharmacognosy, Istanbul, Turkey, turguttaskin@marmara.edu.tr 5Marmara University, Vocational School of Health Services, Department of Medical Services and Techniques, Istanbul, Turkey, szgnaydemir@gmail.comIntroduction: Today, antioxidants have been using diversely in various medical conditions. Therefore, there are growing interest for discovering and developing more effective and safer antioxidants derived from natural sources. Certain Micromeria species were identified as a rich source of antioxidant agents (1). This study aimed to investigate possible antioxidant activity and genotoxicity of Micromeria fruticosa subsp brachycalyx in methanol extract both in vitro and in vivo.Materials and Methods: In vitro antioxidant activity of Micromeria fruticosa subsp brachycalyx in methanol extract was evaluated by DPPH, ABTS, FRAP and CUPRAC assays. In vivo analyses performed on Balb/c mice that divided to three groups (n=6 for each group) as control group (%0,04 Carboxymethyl cellulose solution (CMC), 0,1 mL/10 g, p.o.), positive control group (Indomethacin in %0,04 CMC, 10 mg/kg, p.o.) and treatment group (Micromeria extract in %0,04 CMC, 200 mg/kg, p.o.) and each regimen was applied for 10 days. Myeloperoxidase levels were analyzed in mice liver and kidney tissues. Genotoxic effect was determined in mice blood with Comet Technique. The 8-hydroxy-2’-deoxyguanosine was analyzed in mice liver and kidney tissues with commercial ELISA kit. Results: The results showed that plant extract showed stronger DPPH radical scavenging activity, however, lower ABTS, CUPRAC and FRAP values versus to standards. According to the graph, which shows the elapsed time (second) in pain behavior versus time (minute) for each group, treatment group exhibited its efficacy in late phase (20-30 min) similar with indomethacin group. Myeloperoxidase levels in liver tissues of the extract group were significantly lower compared to the control group (p<0.05). Myeloperoxidase levels in kidney tissues of the extract group were not significantly different compared to both control and indomethacin group. Micromeria fruticosa subsp brachycalyx extract exerted no genotoxic effect on DNA similarly both control and indomethacin group.Conclusions: Our study showed that Micromeria fruticosa subsp brachycalyx has a potential antioxidant activity. It has also been contributed to raise awareness to this species in terms of its safety with the obtained results. Therefore, the usage of this species as natural antioxidant source is promising for new drug candidates.Acknowledgements:Not available.References: 1. Salameh N et al., (2020) Screening of Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia Volatile Oils: A Comparative Study of Plants Collected from Different Regions of West. BioMed Research International, 1-7.Publication Metadata only Fabrication, characterization and fibroblast proliferative activity of electrospun Achillea lycaonica-loaded nanofibrous mats(PERGAMON-ELSEVIER SCIENCE LTD, 2019) KABASAKAL, LEVENT; Cam, Muhammet Emin; Cesur, Sumeyye; Taskin, Turgut; Erdemir, Gokce; Kuruca, Durdane Serap; Sahin, Yesim Muge; Kabasakal, Levent; Gunduz, OguzhanThe use of natural compounds such as biocompatible and non-toxic plant extracts, without undesired side effects, in tissue engineering applications, is highly preferred compared to chemical drugs. In this study, the characterization and performance of electrospun Achillea lycaonica-loaded (0.125, 0.250 and 0.500, wt%) poly (lactic acid) (PLA) (8%, w/v) nanofibrous mats for skin tissue engineering were investigated. SEM, FTIR, DSC, and tensile strength test of the electrospun nanofibers have been investigated. Drug releasing test and cell culture study were also carried out. Achillea lycaonica-loaded nanofibrous mats in 0.250 (wt%) and 0.500 (wt%) demonstrated excellent cell compatibility and increased the viability of NIH/3T3 (mouse embryo fibroblast) cells within 72 h. According to the results, Achillea lycaonica-loaded PLA nanofibers have proper tensile strength and controlled release. The working temperature range enlarged for the composites having higher plant extract content. Consequently, Achillea lycaonica-loaded nanofibrous mats have a great potential in skin tissue engineering applications.Publication Metadata only Investigation of antineoplastic activity of achillea nobilis subsp. Neilreichii plant in experimental breast cancer(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Sehlan S. S., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Alan S., Özbaş S., Kabasakal L.Publication Metadata only The effects of some traditional medical plants and beta amyloid protein in cell viability(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Saleh Al-Rabeei M. A., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Özbaş S., Kabasakal L.Publication Metadata only The methanolic extract of Thymus praecox subsp. skorpilii var. skorpilii restores glucose homeostasis, ameliorates insulin resistance and improves pancreatic beta-cell function on streptozotocin/nicotinamide-induced type 2 diabetic rats(ELSEVIER IRELAND LTD, 2019) YAVUZ, AYŞE NUR; Cam, Muhammet Emin; Hazar-Yavuz, Ayse Nur; Yildiz, Sila; Ertas, Busra; Adakul, Betul Ayaz; Taskin, Turgut; Alan, Saadet; Kabasakal, LeventEthnopharmacological relevance: Thymus praecox subsp. skorpilii var. skorpilii (syn. Thymus praecox subsp. jankae (Celak.) Jalas) is consumed as a Turkish folk medicine for the treatment of spasm, sore throat and shortness of breath, also having strong antioxidant activity and the leaves of the plant have been utilized for the treatment of diabetes as the decoction in Turkey. Aim of the study: In the present study, we aimed to investigate the potential mechanism of antidiabetic action of Thymus praecox subsp. skorpilii var. skorpilii methanolic extract (TPSE) on streptozotocin (STZ)/nicotinamide (NA)-induced type 2 diabetic rats. Materials and methods: Sprague Dawley rats were randomly divided into four groups; control, diabetes, TPSE (100 mg/kg b.w, p.o.) and metformin group (400 mg/kg b.w, p.o.). Diabetes was established in all groups except control group by 55 mg/kg STZ (i.p.) for once 15 min after 100 mg/kg NA injection. 3 days after STZ/NA injection, treatments were administered for three weeks and then rats were decapitated; tissue and blood samples were obtained for measuring the level of glucose transporters (both GLUTs and sodium glucose co-transporters (SGLTs)), enzymes related to glucose (Hexokinase (HK), phosphoenolpyruvate carboxykinase (PEPCK), alpha-glucosidase) and lipid metabolism (Acetyl-coenzyme carboxylase (ACC)), AST, ALT, creatinine, insulin, anti-inflammatory (IL-10) and inflammatory (TNF-alpha, IL-1 beta, IL-6) cytokines, AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma (PPAR-gamma) and glucagon like peptide-1 (GLP-1). Histopathological alterations of the pancreas were examined. Results: After three weeks of treatment, TPSE has exhibited a significant reduction of plasma levels of the proinflammatory cytokines. Besides, TPSE treatment elevated plasma insulin levels and normalized blood glucose levels. Moreover, it improved the values of AMPK in liver and GLP-1 in pancreas. Increased a-glucosidase, PEPCK, GLUT-2 and SGLTs levels with the induction of diabetes considerably lowered with TPSE treatment. Especially on SGLT-2, TPSE achieved a more prominent decrease. After the atrophy in Langerhans islets due to