Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Metadata only Investigation of antioxidant and anticonvulsant activity of Hypericum triquetrifolium Turra(2022-01-01) ÇAM, MUHAMMET EMİN; TAŞKIN, TURGUT; Kirmizi Sonmez N. I. , Guler E., ÇAM M. E. , Ermanoglu M., Gurdal B., TAŞKIN T., Omurtag G. Z.© 2022 Marmara University Press.Epilepsy is a state characterized by sudden, recurrent epileptic seizures that are not initiated by an identifiable event. There are various studies has been shown that Hypericum species may be used for their anticonvulsant potentials. Besides, the relationship between anticonvulsant activity and antioxidant effect has already been shown in the literature. In the current study, H. triquetrifolium was investigated for the first time for its potential antioxidant and anticonvulsant potential using in vitro and in vivo test models. H. triquetrifolium extracts were tested with DPPH assay, FRAP assay, copper (II) ion reducing antioxidant capacity assay, and acetylcholinesterase inhibitory activity assay to understand their antioxidant potential. Especially, methanolic extract of H. triquetrifolium was shown the highest antioxidant activity. Moreover, a pentylenetetrazole (PTZ, 80 mg/kg, i.p.)-induced seizure model was conducted to analyze the anticonvulsant activities of H. triquetrifolium extracts in mice. In addition, this study revealed that H. triquetrifolium decreased the ratio of severe seizures and increased the mean onsite of mortality and survival rate in a dose-dependent manner. It is thought that the anticonvulsant effect may be either related to the antioxidant potential of H. triquetrifolium or its interference in the GABAergic system.Publication Metadata only Evaluation of antioxidant, analgesic, anti-inflammatory and antispasmodic activity and genotoxic effect of micromeria fruticosa subsp brachycalyx in vitro and in vivo(2021-07-22) KABASAKAL, LEVENT; TAŞKIN, TURGUT; AYDEMİR, SEZGİN; BECEREN, AYFER; Çelikkol I., Beceren A., Kabasakal L., Taşkın T., Aydemir S.EVALUATION OF ANTIOXIDANT, ANALGESIC AND ANTIINFLAMMAUTORY ACTIVITY AND GENOTOXIC EFFECT OF MICROMERIA FRUTICOSA SUBSP BRACHYCALYX IN VITRO AND IN VIVO1Celikkol I., 2Beceren A., 3Kabasakal L., 4Taskin T., 5Aydemir S.1Marmara University, Institute of Health Sciences, Department of Pharmaceutical Toxicology Istanbul, Turkey, celikkolisik@gmail.com 2Marmara University, Department of Pharmaceutical Toxicology, Istanbul, Turkey, ayfertozan@hotmail.com 3Marmara University, Department of Pharmacology, Istanbul, Turkey, lkabasakal@gmail.com 4Marmara University, Department of Pharmacognosy, Istanbul, Turkey, turguttaskin@marmara.edu.tr 5Marmara University, Vocational School of Health Services, Department of Medical Services and Techniques, Istanbul, Turkey, szgnaydemir@gmail.comIntroduction: Today, antioxidants have been using diversely in various medical conditions. Therefore, there are growing interest for discovering and developing more effective and safer antioxidants derived from natural sources. Certain Micromeria species were identified as a rich source of antioxidant agents (1). This study aimed to investigate possible antioxidant activity and genotoxicity of Micromeria fruticosa subsp brachycalyx in methanol extract both in vitro and in vivo.Materials and Methods: In vitro antioxidant activity of Micromeria fruticosa subsp brachycalyx in methanol extract was evaluated by DPPH, ABTS, FRAP and CUPRAC assays. In vivo analyses performed on Balb/c mice that divided to three groups (n=6 for each group) as control group (%0,04 Carboxymethyl cellulose solution (CMC), 0,1 mL/10 g, p.o.), positive control group (Indomethacin in %0,04 CMC, 10 mg/kg, p.o.) and treatment group (Micromeria extract in %0,04 CMC, 200 mg/kg, p.o.) and each regimen was applied for 10 days. Myeloperoxidase levels were analyzed in mice liver and kidney tissues. Genotoxic effect was determined in mice blood with Comet Technique. The 8-hydroxy-2’-deoxyguanosine was analyzed in mice liver and kidney tissues with commercial ELISA kit. Results: The results showed that plant extract showed stronger DPPH radical scavenging activity, however, lower ABTS, CUPRAC and FRAP values versus to standards. According to the graph, which shows the elapsed time (second) in pain behavior versus time (minute) for each group, treatment group exhibited its efficacy in late phase (20-30 min) similar with indomethacin group. Myeloperoxidase levels in liver tissues of the extract group were significantly lower compared to the control group (p<0.05). Myeloperoxidase levels in kidney tissues of the extract group were not significantly different compared to both control and indomethacin group. Micromeria fruticosa subsp brachycalyx extract exerted no genotoxic effect on DNA similarly both control and indomethacin group.Conclusions: Our study showed that Micromeria fruticosa subsp brachycalyx has a potential antioxidant activity. It has also been contributed to raise awareness to this species in terms of its safety with the obtained results. Therefore, the usage of this species as natural antioxidant source is promising for new drug candidates.Acknowledgements:Not available.References: 1. Salameh N et al., (2020) Screening of Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia Volatile Oils: A Comparative Study of Plants Collected from Different Regions of West. BioMed Research International, 1-7.Publication Metadata only Antioxidant, antimicrobial, anti-urease and cytotoxic activities of various extracts from Scutellaria sibthorpii endemic to Cyprus(2022-07-01) TAŞKIN, TURGUT; Toplan G. G., TAŞKIN T., Oztas E., Hacioglu M., Tufan S., SARIYAR G., Mat A.Lamiaceae is one of the largest families in the plant kingdom. Including the genus Scutellaria whose species are used in traditional medicine in various countries for prevention and also the treatment of several disorders. In vitro biological activities of the various extracts of the aerial parts from Scutellaria sibthorpii were investigated in the present study. Antioxidant activity of the extracts was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS assays and ferric reducing antioxidant power (FRAP) assays. Cytotoxic potential and anti-urease activities of the extracts were also determined. Spectrophotometric analysis was used to establish the total phenol content of the extracts. The antibacterial activities of extracts were assessed by minimal inhibitory concentration (MIC) against seven bacteria and Candida albicans. Regarding the results, infusion showed considerable antioxidant properties in three method (IC50 value 0.133 +/- 0.0005(DPPH), 26.54 +/- 0.05 mM trolox/mg extract(ABTS), 8.76 +/- 0.1 mM Fe+2/mg extract (FRAP)). Other extracts exhibited high-to-moderate antioxidant effects. Among the studied samples, the infusion had the highest total phenolic content (0.0295 +/- 0.0002 mg GAE/mg extract). According to cytotoxicity results, n-hexane extract had strong cytotoxic effects against PC-3 and NIH/3T3 cell line with IC50 values of 330.40 mu g/mL and 340.85 mu g/mL, respectively. In the antibacterial activity screening, the ethyl acetate extract showed higher activity against Enterecocus facecalis, Klebsiella pneumoniae, Proteus mirabilis with 625 mu g/mL MIC value. According to the research carried out, it would seem that this is the first study on screening biological activities of extracts from Scutellaria sibthorpii. These findings indicate that these endemic species from Cyprus could be used in phytopharmaceutical preparation.Publication Metadata only Investigation of antineoplastic activity of achillea nobilis subsp. Neilreichii plant in experimental breast cancer(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Sehlan S. S., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Alan S., Özbaş S., Kabasakal L.Publication Metadata only Investigation of antioxidant and antibicrobial activities of muscari armeniacum(2023-05-04) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; EMRE, GİZEM; TAŞKIN, TURGUT; Avcı E., Rayaman E., Yılmaz B. N., Emre G., Taşkın T.Publication Metadata only The effects of some traditional medical plants and beta amyloid protein in cell viability(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Saleh Al-Rabeei M. A., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Özbaş S., Kabasakal L.