Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Metadata only Fabrication, characterization and fibroblast proliferative activity of electrospun Achillea lycaonica-loaded nanofibrous mats(PERGAMON-ELSEVIER SCIENCE LTD, 2019) KABASAKAL, LEVENT; Cam, Muhammet Emin; Cesur, Sumeyye; Taskin, Turgut; Erdemir, Gokce; Kuruca, Durdane Serap; Sahin, Yesim Muge; Kabasakal, Levent; Gunduz, OguzhanThe use of natural compounds such as biocompatible and non-toxic plant extracts, without undesired side effects, in tissue engineering applications, is highly preferred compared to chemical drugs. In this study, the characterization and performance of electrospun Achillea lycaonica-loaded (0.125, 0.250 and 0.500, wt%) poly (lactic acid) (PLA) (8%, w/v) nanofibrous mats for skin tissue engineering were investigated. SEM, FTIR, DSC, and tensile strength test of the electrospun nanofibers have been investigated. Drug releasing test and cell culture study were also carried out. Achillea lycaonica-loaded nanofibrous mats in 0.250 (wt%) and 0.500 (wt%) demonstrated excellent cell compatibility and increased the viability of NIH/3T3 (mouse embryo fibroblast) cells within 72 h. According to the results, Achillea lycaonica-loaded PLA nanofibers have proper tensile strength and controlled release. The working temperature range enlarged for the composites having higher plant extract content. Consequently, Achillea lycaonica-loaded nanofibrous mats have a great potential in skin tissue engineering applications.Publication Open Access The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum(UNIV SAO PAULO, CONJUNTO QUIMICAS, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Gezmis, Tugba; Cam, Muhammet Emin; Taskin, Duygu; Celik, Berna Ozbek; Senkardes, Ismail; Suzgec-Selcuk, SevdaIn Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones arc endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatmy and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinestemse activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.Publication Open Access The Influence of Different Extraction Methods/Solvents on Composition, Biological Activities and ADMET Predictions of Phenolics in Tribulus terrestris(INST TECNOLOGIA PARANA, 2021) TAŞKIN, TURGUT; Taskin, Duygu; Yilmaz, Beyza Nur; Taskin, Turgut; Omurtag, Gulden ZehraTribulus terrestris is a plant that has medical importance in treating diseases such as cancer, urinary tract infections, kidney stones, rheumatism, and hypertension. The aim of this study is to examine the effects of extraction methods/solvents on the biological activity and chemical content of the plant and then to determine ADMET predictions of phenolics that were analysed quantitatively using HPLC-DAD in the plant. Maceration methanol (IC50:0.277 mg/mL) and chloroform (0.263 mMFeSO(4)/mg extract) extracts were found to have the strongest DPPH radical scavenging and iron (III) ion reducing activity, respectively. It was determined that Soxhlet methanol (0.0225 mMTE/mg extract) and Soxhlet chloroform (1.360 mMTE/mg extract) extracts exhibited stronger radical cation (ABTS.+) scavenging and cupric ion reducing activity compared to other extracts, respectively. It was found that ultrasonic bath methanol extracts showed the highest anti-urease (21.47%) and calcium oxalate anti-crystallization (71.54%, 80.52%) activity. It was also found that Soxhlet methanol extract (66.763%) has strong acetylcholinesterase enzyme inhibition capacity compared to other extracts. Pyrocatechol, vanillic acid, vanillin, rutin and rosmarinic acid were analysed by HPLC-DAD in the plant. Moreover, it was determined that methanol extracts obtained using soxhlet, ultrasonic bath, and maceration contained the highest amount of rutin, pyrocatechol, and rutin, respectively. ADMET predictions of phenolics show that these compounds are easily absorbed and do not have toxic effects, suggesting that this species may be used as a natural medicinal and nutritional source in the future after detailed analysis tests.Publication Metadata only Characterization of the Biological Activity and Phenolics in Achillea lycaonica(TAYLOR & FRANCIS INC, 2018) RAYAMAN, ERKAN; Taskin, Turgut; Taskin, Duygu; Rayaman, Erkan; Dikpinar, Tugce; Suzgec-Selcuk, Sevda; Arabaci, TuranThe purpose of this study was to comparatively evaluate for the first time the antioxidant, antimicrobial, and antiurease effects of A. lycaonica extracts obtained using three extraction methods and five solvents followed by identification of the phenolic compounds obtained by Soxhlet extraction with ethyl acetate and maceration with chloroform that showed the highest total phenolic and flavonoid contents with antioxidant and antiurease activity. The phenolic compounds were identified to be chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, salicylic acid, luteolin, quercetin, naringenin, apigenin, and 8-hydroxy- salvigenin. Among the solvent extraction procedures, Soxhlet approach was found to have higher recoveries than other approaches. The results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays showed that the 1: 1 ethanol-water Soxhlet, ethanol ultrasonic bath, and ethyl acetate Soxhlet extraction methods had the highest DPPH antioxidant activities. The ethyl acetate Soxhlet extraction method showed the highest 2, 2'-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) antioxidant activity. In addition, the chloroform maceration extraction method exhibited the highest antiurease activity. Therefore, 1: 1 Soxhlet ethanol-water, ultrasonic bath ethanol, and Soxhlet ethyl acetate extracts may be used in medicine and food production as natural antioxidant sources. In addition, maceration with chloroform may provide a potential antiurease agent.Publication Metadata only Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial(BENTHAM SCIENCE PUBL, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Akkilic, Yeliz; Celik, Berna Ozbek; Senkardes, Ismail; Ozakpinar, Ozlem B.Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant's different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and tlavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using mnicrodilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (('UPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicatis strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.