Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Metadata only Evaluation of antioxidant, analgesic, anti-inflammatory and antispasmodic activity and genotoxic effect of micromeria fruticosa subsp brachycalyx in vitro and in vivo(2021-07-22) KABASAKAL, LEVENT; TAŞKIN, TURGUT; AYDEMİR, SEZGİN; BECEREN, AYFER; Çelikkol I., Beceren A., Kabasakal L., Taşkın T., Aydemir S.EVALUATION OF ANTIOXIDANT, ANALGESIC AND ANTIINFLAMMAUTORY ACTIVITY AND GENOTOXIC EFFECT OF MICROMERIA FRUTICOSA SUBSP BRACHYCALYX IN VITRO AND IN VIVO1Celikkol I., 2Beceren A., 3Kabasakal L., 4Taskin T., 5Aydemir S.1Marmara University, Institute of Health Sciences, Department of Pharmaceutical Toxicology Istanbul, Turkey, celikkolisik@gmail.com 2Marmara University, Department of Pharmaceutical Toxicology, Istanbul, Turkey, ayfertozan@hotmail.com 3Marmara University, Department of Pharmacology, Istanbul, Turkey, lkabasakal@gmail.com 4Marmara University, Department of Pharmacognosy, Istanbul, Turkey, turguttaskin@marmara.edu.tr 5Marmara University, Vocational School of Health Services, Department of Medical Services and Techniques, Istanbul, Turkey, szgnaydemir@gmail.comIntroduction: Today, antioxidants have been using diversely in various medical conditions. Therefore, there are growing interest for discovering and developing more effective and safer antioxidants derived from natural sources. Certain Micromeria species were identified as a rich source of antioxidant agents (1). This study aimed to investigate possible antioxidant activity and genotoxicity of Micromeria fruticosa subsp brachycalyx in methanol extract both in vitro and in vivo.Materials and Methods: In vitro antioxidant activity of Micromeria fruticosa subsp brachycalyx in methanol extract was evaluated by DPPH, ABTS, FRAP and CUPRAC assays. In vivo analyses performed on Balb/c mice that divided to three groups (n=6 for each group) as control group (%0,04 Carboxymethyl cellulose solution (CMC), 0,1 mL/10 g, p.o.), positive control group (Indomethacin in %0,04 CMC, 10 mg/kg, p.o.) and treatment group (Micromeria extract in %0,04 CMC, 200 mg/kg, p.o.) and each regimen was applied for 10 days. Myeloperoxidase levels were analyzed in mice liver and kidney tissues. Genotoxic effect was determined in mice blood with Comet Technique. The 8-hydroxy-2’-deoxyguanosine was analyzed in mice liver and kidney tissues with commercial ELISA kit. Results: The results showed that plant extract showed stronger DPPH radical scavenging activity, however, lower ABTS, CUPRAC and FRAP values versus to standards. According to the graph, which shows the elapsed time (second) in pain behavior versus time (minute) for each group, treatment group exhibited its efficacy in late phase (20-30 min) similar with indomethacin group. Myeloperoxidase levels in liver tissues of the extract group were significantly lower compared to the control group (p<0.05). Myeloperoxidase levels in kidney tissues of the extract group were not significantly different compared to both control and indomethacin group. Micromeria fruticosa subsp brachycalyx extract exerted no genotoxic effect on DNA similarly both control and indomethacin group.Conclusions: Our study showed that Micromeria fruticosa subsp brachycalyx has a potential antioxidant activity. It has also been contributed to raise awareness to this species in terms of its safety with the obtained results. Therefore, the usage of this species as natural antioxidant source is promising for new drug candidates.Acknowledgements:Not available.References: 1. Salameh N et al., (2020) Screening of Antioxidant and Antimicrobial Activity of Micromeria fruticosa serpyllifolia Volatile Oils: A Comparative Study of Plants Collected from Different Regions of West. BioMed Research International, 1-7.Publication Metadata only Investigation of antineoplastic activity of achillea nobilis subsp. Neilreichii plant in experimental breast cancer(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Sehlan S. S., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Alan S., Özbaş S., Kabasakal L.Publication Metadata only Investigation of antioxidant and antibicrobial activities of muscari armeniacum(2023-05-04) RAYAMAN, ERKAN; YILMAZ, BEYZA NUR; EMRE, GİZEM; TAŞKIN, TURGUT; Avcı E., Rayaman E., Yılmaz B. N., Emre G., Taşkın T.Publication Open Access Investigation on biological activities of Melissa officinalis subsp. altissima(2022-05-23) RAYAMAN, ERKAN; ŞENKARDEŞ, İSMAİL; TAŞKIN, TURGUT; Koç G., Rayaman E., Özdatlı Kurtuluş Ş., Ermanoğlu M., Yılmaz B. N. , Şenkardeş İ., Taşkın T.The genus Melissa is used as antibacterial, sedative, digestive aid, carminative, diaphoretic and bile secretion enhancer. It is included in the composition of the preparations used during nervous digestive system disorders, psychosomatic heart disorders and migraine. The flowering branches and leaves of Melissa officinalis L. subsp. altissima. are used as carminative, sedative and its antipyretic effects. It is also used in vascular occlusion, stomach aches, heart ailments, kidney inflammation, cholesterol, blood pressure, and diabetes. According to our knowledge there are not enough studies on these species grown in Turkey. The aim of this study is to examine the antioxidant, cytotoxic, anticholinesterase, anti-urease and antimicrobial activities of plant’ various extracts obtained by using the sequential maceration method. In the determination of antimicrobial activity well diffusion method on 6 gram negative, 4 gram positive and 3 yeast strains was used. DPPH, CUPRAC and FRAP techniques were used to examine the antioxidant properties of plant\" extracts. The extracts\" anticholinesterase, cytotoxic and anti-urease activity were determined using the Ellman, MTT and Indophenol techniques, respectively. In addition, the phenolic contents of the extracts were determined by FCR method. It was found that petroleum ether extract of the plant was effective against Enterecoccus faecalis and Staphylococcus aureus strains and ethyl acetate extract were effective against Staphylococcus aureus strains. The methanol extract of the plant exhibited the highest antioxidant activity when compared to other extracts. It was determined that the petroleum ether extract of Melissa officinalis L. subsp. altissima has stronger acetylcholinesterase and urease enzyme inhibition potential than its other extracts. Furthermore, in this study the ethyl acetate extract of the plant was found to have high cytotoxic activity.Publication Metadata only The effects of some traditional medical plants and beta amyloid protein in cell viability(2019-07-03) YAVUZ, AYŞE NUR; EKENTOK ATICI, CEYDA; TAŞKIN, TURGUT; ÖZBAŞ, SUNA; KABASAKAL, LEVENT; Saleh Al-Rabeei M. A., Yavuz A. N., Ekentok Atıcı C., Taşkın T., Özbaş S., Kabasakal L.Publication Open Access In vitro biological activities of different extracts from alcea dissecta(2022-03-01) TAŞKIN, TURGUT; DOĞAN, AHMET; TAŞKIN T., Kahvecioglu D., Turkoglu E. A., DOĞAN A., Kuzu M.Objective: Alcea genus belongs to Malvaceae family and this genus is represented by 85 taxa in the world and 21 taxa in the Flora of Turkey. The flowers of Alcea genus contain plenty of mucilage and are used for the treatment of respiratory diseases such as asthma and cough. Alcea dissecta is known as ‘Govik, Hiro, Hero’ in Turkey, and the flowers and leaves of this species have been used in the treatment of asthma, injury, colds, and gastrointestinal diseases in Turkey. To the best of our knowledge, there is no report on the effect of extraction methods on the biological activity of this plant. In addition, although this species is being used as a medical plant, there is no study of the antioxidant, antiurease, esterase, and anticholinesterase activity of the plant. Therefore, the aim of this study was to evaluate in vitro antioxidant, anti-urease, esterase, anticholinesterase activities of Alcea dissecta using a variety of extracts. Methods: The antioxidant activities of different extracts were examined by DPPH, ABTS, FRAP, and CUPRAC methods. The total phenolic compounds contained in the extracts were determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease and anticholinesterase activities of different extracts were evaluated by indophenol and Ellman methods respectively. In addition, esterase activities of plant extracts were determined. Results: In the present study, ethanol:water (1:1, v/v) and chloroform extracts obtained maceration method showed stronger DPPH and ABTS radical scavenging activity than other extracts. The chloroform extract obtained Soxhlet method was found to have higher FRAP and CUPRAC values than other extracts. It was also found that the ethanol extract obtained maceration method showed the most potent anti-urease and anticholinesterase activity. According to the results, the strongest inhibitory effect on both hCA I and II isoenzymes was shown by the petroleum ether extract obtained Soxhlet method. Conclusion: As a result, it was determined that different plant extracts have antioxidant, anti-urease, esterase, anticholinesterase activities. In addition, the data obtained from this study will shed light on future research on the biological activities of this species.Publication Open Access The methanolic extracts of Teucrium polium L. and Micromeria fruticosa (L.) Druce subsp. brachycalyx P. H. Davis improve diabetes in streptozotocin/nicotinamide-induced type 2 diabetic female Sprague Dawley rats(2023-12-01) ÇAM, MUHAMMET EMİN; YAVUZ, AYŞE NUR; TAŞKIN, TURGUT; KABASAKAL, LEVENT; ELÇİOĞLU, HATİCE KÜBRA; Sermet S., ÇAM M. E., YAVUZ A. N., TAŞKIN T., Kabatas G. S., YAZIR Y., KABASAKAL L., ELÇİOĞLU H. K.Background: Teucrium polium and Micromeria fruticosa subspecies are known to be used in traditional medicine for diabetes treatment. Objective: The present study aimed to examine the potential antidiabetic effects and the mechanisms of antidiabetic actions of Teucrium polium L. methanolic extract (TP) and Micromeria fruticosa (L.) Druce subsp. brachycalyx P. H. Davis methanolic extract (MF) on rats with type 2 diabetes mellitus (DM). Methods: Streptozotocin (STZ) and nicotinamide (NA) were injected intraperitoneally to induce type 2 diabetes mellitus (T2DM) in rats. Plant extracts’ antidiabetic mechanisms of action were investigated with biochemical analyses and histopathological analyses performed. Results: TP and MF treatments reduced the blood glucose levels compared to the untreated diabetic rats. TP reduced TNF-α levels in serum, increased insulin levels in serum and pancreas, reduced SGLT-2 levels in kidneys, reduced GLUT-2 levels in the ileum; and MF reduced TNF-α levels, and increased insulin levels in serum and pancreas, increased GLP-1 levels, and reduced GLUT-2 levels in the ileum, and reduced SGLT-2 levels in kidneys. Treatments improved the histopathological results in the pancreas, kidney, and liver. Conclusions: The results presented in this study demonstrated that TP and MF both have potential antidiabetic effects and may be effective in T2DM treatment.Publication Metadata only Fabrication and characterization of achillea Iycaonica- loaded poly(Lactic Acid) nanofibers(2018-09-14) ÇAM, MUHAMMET EMİN; CESUR, SÜMEYYE; TAŞKIN, TURGUT; GÜNDÜZ, OĞUZHAN; Çam M. E., Cesur S., Taşkın T., Kabasakal L., Gündüz O.Publication Open Access The Biological Activities of Endemic Ballota pseudodictamnus subsp. lycia.(2022-05-23) RAYAMAN, ERKAN; TAŞKIN, TURGUT; Akduman B., Rayaman E., Yılmaz B., Ermanoğlu M., Gürdal B., Taşkın T.The genus Ballota L. (Lamiaceae) includes species known as boz ot, bal otu, nemnem otu and have medicinal uses among the people. Additionally, it known to be used by the public in the treatment of cough, asthma, headache, nausea, hemorrhoids, wounds and burns. Ballota taxa have various phytochemical classes such as essential oils, iridoids, saponins, terpenoids, flavonoids, phenolic acids, tannins, and organic acids. Ballota pseudodictamnus subsp. lycia is one of the endemic species growing in southwestern Turkey. As a result of our research on the literature a limited number of studies have been found on the biological activities of this species. The aim of this study is to examine the antioxidant, antimicrobial, anticholinesterase and anti-urease activities of different extracts (petroleum ether, chloroform and methanol) obtained from the aerial part of B. pseudodictamnus subsp. lycia. DPPH, CUPRAC, and FRAP techniques were used to examine the antioxidant properties of plant\" extracts. The extracts\" anti-urease, anticholinesterase and antimicrobial activity were determined using the Indophenol, Ellman and agar well diffusion method techniques, respectively. While the petroleum ether extract obtained from plant, was found to be effective against S. aureus, S.epidermidis and E. faecalis strains; its chloroform extract was effective against S.aureus and S.epidermidis strains and its methanol extract was found to be effective against Acinetobacter baumannii strains. It was found that the plant’ methanol extract showed the highest antioxidant activity when compared to the other extracts.Publication Metadata only Investigation of biological activities of Paranchia kurdica subsp. kurdica var. kurdica and analysis of major phytochemicals by HPLC-DAD(2021-11-24) TAŞKIN, TURGUT; RAYAMAN, ERKAN; DOĞAN, AHMET; Ermanoğlu M., Taşkın D., Taşkın T., Rayaman E., Doğan A.