Person: ŞENKARDEŞ, İSMAİL
Loading...
Email Address
Birth Date
Research Projects
Organizational Units
Job Title
Last Name
ŞENKARDEŞ
First Name
İSMAİL
Name
39 results
Search Results
Now showing 1 - 10 of 39
Publication Metadata only Chemical characterization and pharmacological profile of tribulus terrestris extracts: A novel source of cosmeceuticals and pharmaceuticals(2023-04-01) ŞENKARDEŞ, İSMAİL; UYSAL Ş., ŞENKARDEŞ İ., Jekő J., Cziáky Z., Zengin G.© 2023 Elsevier LtdTribulus terrestris is widely used in traditional medicine and in dietary supplements. Therefore, this research aims to obtain comprehensive information on antioxidant properties, enzyme inhibitory activity and chemical composition of T. terrestris leaves. HPLC-MS/MS was used to further extensively characterize the chemical composition of T. terrestris extracts. 27, 75, and 51 chemical components were identifed in ethyl acetate, methanol and water extract, respectively. The radical scavenging activity results showed that water extract had the strongest acvitiy (DPPH: 30.50 mgTE/g, ABTS: 44.49 mgTE/g). The reducing power activity was assessed by FRAP and CUPRAC assays. The highest CUPRAC activity is shown by the ethyl acetate extract (79.01 mgTE/g). Water extract showed the highest metal chelating activity compared with other extract. In general, the highest antioxidant capacity were obtained in the water extract. Ethyl acetate extract exhibited the highest enzyme inhibition activity (AChE: 1.56 mgGAE/g; BChE: 2.14 mgGALAE/g; α-amylase: 0.73 mmolACE/g; Tyrosinase: 30.14 mgKAE/g) compared with other extracts. This research provides a scientific basis for the development of natural antioxidant and enzyme inhibiton sources from T. terrestris.Publication Metadata only Chemical composition and bio-functional perspectives of Erica arborea L. extracts obtained by different extraction techniques: Innovative insights(ELSEVIER, 2019) DOĞAN, AHMET; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Stupar, Alena; Bulut, Gizem; Senkardes, Ismail; Dogan, Ahmet; Seebaluck-Sandoram, Roumita; Rengasamy, Kannan R. R.; Sinan, Kouadio Ibrahime; Mahomoodally, Mohamad FawziErica arborea L., also known as Estrella Gold, is traditionally used for several purposes. In this research, five different extraction techniques: accelerated solvent extraction (ASE), microwave-assisted extraction (MAE), maceration (MAC), soxhlet (SOE) and ultrasound-assisted extraction (UAE) were used to compare the total phenolic, flavonoids, total antioxidant activity and enzymatic activities of E. arborea extracts obtained different extraction techniques. The total phenolic and flavonoid contents were in the order of ASE > MAE > SOE > MAC > UAE. All extracts showed antioxidant, anticholinesterase, anti-tyrosinase and anti-diabetic activities. A highly sensitive method using ultra-high-pressure liquid chromatography coupled with linear ion trap-Orbitrap tandem mass spectrometry (UHPLC-LTQ-Orbitrap-MS) has been used for the qualitative analysis of obtained extracts. Seventy-two polyphenolic compounds were identified in all extracts. However, 20 components were quantified among the extracts. ASE was found to be a better extraction technique as compared to the other extraction techniques. E. arborea can be exploited in the discovery of bioactive natural products for the treatment of Alzheimer's disease, diabetes and pigmentation problems.Publication Metadata only Utilisation of Rhododendron luteum Sweet bioactive compounds as valuable source of enzymes inhibitors, antioxidant, and anticancer agents(PERGAMON-ELSEVIER SCIENCE LTD, 2020) ŞENKARDEŞ, İSMAİL; Mahomoodally, Mohamad Fawzi; Sieniawska, Elwira; Sinan, Kouadio Ibrahime; Picot-Allain, Marie Carene Nancy; Yerlikaya, Serife; Baloglu, Mehmet Cengiz; Altunoglu, Yasemin Celik; Senkardes, Ismail; Rengasamy, Kannan R. R.; Zengin, GokhanEthnobotanical evidences report the use of Rhododendron luteum Sweet (Ericaceae) in traditional medicinal systems. However, R. luteum has been associated to the occurrence of 'mad honey' poisoning. In the present study, the ethyl acetate, methanol, and water extracts of R. luteum were investigated for their in vitro antioxidant, enzyme inhibition, and cytotoxic properties. The cytotoxicity of R. luteum extracts on A549 lung cancer cell line was evaluated using MTT cell viability assay. Besides, HPLC-ESI-MSn approach was employed to elucidate the secondary metabolite profiles of R. luteum in order to establish any structure-activity relationship. Methanol and water extracts of R. luteum possessed highest radical scavenging and reducing properties while the ethyl acetate extract showed highest metal chelating properties. In terms of enzyme inhibition, the methanol and ethyl acetate extracts of R. luteum, possessing epigallocatechin, were active inhibitors of cholinesterase enzymes, a-glucosidase, and tyrosinase. Water extract caused growth inhibition of A549 cells with 207.2 mu g/ml IC50 value. Though R. luteum has received little scientific attention due to the occurrence of grayanotoxins in the plant, however, data presented in this work shows promising biological activity of R. luteum and highlighted its role as a potential source of antioxidant and key enzyme inhibitors.Publication Metadata only Multidirectional biological investigation and phytochemical profile of Rubus sanctus and Rubus ibericus(PERGAMON-ELSEVIER SCIENCE LTD, 2019) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Ferrante, Claudio; Senkardes, Ismail; Gevrenova, Reneta; Zheleva-Dimitrova, Dimitrina; Menghini, Luigi; Orlando, Giustino; Recinella, Lucia; Chiavaroli, Annalisa; Leone, Sheila; Brunetti, Luigi; Picot-Allain, Carene Marie Nancy; Rengasamy, Kannan R. R.; Mahomoodally, Mohamad FawziIn the present study, the biological properties, including, the enzyme inhibitory and antioxidant activities, as well as, the phytochemical profile of the ethyl acetate, methanol, and water extracts of Rubus sanctus Schreb. and Rubus ibericus Juz. leaves were determined using in vitro bioassays. Wide range of phytochemicals, including, hydroxybenzoic acids, hydroxycinnamic acids, acylquinic acids, ellagitannins, flavonoids, and triterpenoid saponins were determined using UHPLC-ESI/HRMS technique. The ethyl acetate and methanol extracts of the studied Rubus species effectively inhibited acetyl and butyryl cholinesterase. On the other hand, R. sanctus water extract showed low inhibition against alpha-amylase and prominent inhibitory action against alpha-glucosidase. Data collected from this study reported the radical scavenging and reducing potential of the studied Rubus species. Investigation of the protective effects of the different extracts of R. sanctus and R. ibericus in experimental model of ulcerative colitis was performed. The extracts were also tested on spontaneous migration of human colon cancer cells (HCT116) in wound healing experimental paradigm. Only R. sanctus methanol extract inhibited spontaneous HCT116 migration in the wound healing test. Our results suggested that R. sanctus and R. ibericus may be potential candidates as sources of biologically-active compounds for the development of nutraceuticals, pharmaceuticals, and/or cosmetics.Publication Open Access Chemical Characterization and Bioactive Properties of Different Extracts from Fibigia clypeata, an Unexplored Plant Food(MDPI, 2020-06-01) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Mahomoodally, Mohamad Fawzi; Rocchetti, Gabriele; Lucini, Luigi; Sieniawska, Elwira; Swiatek, Lukasz; Rajtar, Barbara; Polz-Dacewicz, Malgorzata; Senkardes, Ismail; Aktumsek, Abdurrahman; Picot-Allain, Marie Carene Nancy; Montesano, DomenicoFibigia clypeata(L.) Medik. is a poorly studied plant species belonging to the Brassicaceae family, and usually used as cress in the salads. The current investigation aimed at assessing the antioxidant potential and inhibitory activity of ethyl acetate, methanol, and aqueous extracts ofF. clypeataagainst key enzymes targeted in the management of type II diabetes (alpha-amylase and alpha-glucosidase), Alzheimer's disease (acetylcholinesterase and butyrylcholinesterase), and skin hyperpigmentation (tyrosinase). Cytotoxicity of the extracts was also determined using normal VERO and cancer FaDu and SCC-25 cell lines. Besides, LC-MS was employed to investigate the detailed phytochemical profiles of the extracts. The methanol extract showed potent enzyme inhibitory activity (4.87 mg galantamine equivalent/g, 3.52 mg galantamine equivalent/g, 126.80 mg kojic acid equivalent/g, and 24.68 mg acarbose equivalent/g, for acetylcholinesterase, butyrylcholinesterase, tyrosinase, and alpha-glucosidase, respectively) and antioxidant potential (96.52, 109.10, 154.02, and 104.85 mg trolox equivalent/g, for DPPH, ABTS, CUPRAC, and FRAP assays, respectively). Interestingly, caffeic acid-O-hexoside derivative, caffeyl alcoholO-glucopyranoside, and ferulic acid derivative were identified in all extracts.F. clypeataextracts showed no cytotoxicity towards VERO cell line and a weak cytotoxic potential against FaDu and SCC-25 cell lines. Interesting scientific evidence gathered from the present study support further investigation onF. clypeatain the view of designing and developing a novel therapeutic agent for the management of Alzheimer's disease, type II diabetes, skin hyperpigmentation problems, as well as cancer.Publication Open Access An analytical framework combining online high-performance liquid chromatography methodologies and biological properties of different extracts of Leonurus cardiaca(2023-01-01) ŞENKARDEŞ, İSMAİL; Nilofar N., EYUPOĞLU O. E., Nazzaro F., Fratianni F., Ahmed S., Ferrante C., ŞENKARDEŞ İ., Zengin G.Little or no information is available concerning online high-performance liquid chromatography (HPLC) antioxidants and the antibiofilm effect of Leonurus cardiaca. Five distinct extractions of methanolic, ethyl acetate, dichloromethane, hexane, and water were obtained from L. cardiaca. In the online-HPLC-antioxidant analysis of all examined samples, rosmarinic acid emerged as the primary antioxidant, registering concentrations ranging from 6 to 15 ppm at wavelengths of 517 and 734 nm. Notably, the water extract exhibited robust antioxidant activity In vitro. Regarding acetylcholinesterase and butrylcholinesterase inhibition, the n-hexane extract exhibited superior inhibition with values of 3.08 and 5.83 galanthamine equivalent, respectively. Except for the water extract, all tested extracts (at a concentration of 20 μg/mL) exhibited substantial inhibitory activity against biofilm formation, in many cases superior to 80%, and reached even 94.52% against Escherichia coli. Although less vigorous, the extracts also acted against the mature biofilm (inhibition up 76.50% against Staphylococcus aureus). They could work against the metabolism inside an immature and mature biofilm, with inhibition percentages up to 93.18% (vs. Pseudomonas aeruginosa) and 76.50% (vs. Acinetobacter baumannii), respectively. Considering its significant antioxidants, enzyme inhibition, and antimicrobial activity, L. cardiaca emerges as a promising candidate for therapeutic potential.Publication Open Access In vitro investigation of antimicrobial, enzyme inhibitory and free radical scavenging activities of Inula salicina L.(2022-09-01) YILDIRIM, AYBENİZ; ŞEN, ALİ; ŞENKARDEŞ, İSMAİL; BİTİŞ, LEYLA; Yıldırım A., ŞEN A., HACIOĞLU M., BİRTEKSÖZ TAN A. S. , ŞENKARDEŞ İ., BİTİŞ L.In this study, in vitro biological activities and total phenol/flavonoid contents of methanol extract (ISM) and its hexane (ISH), chloroform (ISC), ethyl acetate (ISEA) and aqueous methanol (ISAM) fractions obtained from aerial parts of Inula salicina were investigated. ISEA showed the highest antioxidant activity against DPPH and ABTS radicals with an IC50 value of 0.014 mg ml-1 for both assays. ISEA exhibited a good anti-inflammatory activity with an IC50 value of 0.060 mg ml-1 . ISEA was found to exhibit a moderate level of antidiabetic activity against α amylase enzyme with an IC50 value of 0.290 mg ml-1 . ISEA and ISM presented low and moderate inhibitory activity against acetylcholinesterase and butyrylcholinesterase enzymes with IC50 values of 0.577 and 0.279 mg ml-1 , respectively. ISC with MIC values of 78 and 156 µg ml-1 displayed a significant antimicrobial activity against Staphylococcus aureus and S. epidermidis, respectively. Almost all extracts had moderate effect against Candida species. The highest total phenolic and flavonoid contents were determined in ISEA with 574.8 mg GAE (gallic acid equivalent) g -1 extract and 30.48 mg QE (quercetin equivalent) g -1 extract, respectively. These results showed that ISEA had a good antioxidant and anti-inflammatory activity with moderate α-amylase and butyrylcholinesterase inhibitory activity. Also, ISC exhibited a significant antimicrobial activity against Staphylococcus species.Publication Open Access Phytochemical Analysis, Network Pharmacology and in Silico Investigations on Anacamptis pyramidalis Tuber Extracts(MDPI, 2020-05-22) ŞENKARDEŞ, İSMAİL; Mahomoodally, Mohamad Fawzi; Picot-Allain, Marie Carene Nancy; Zengin, Gokhan; Llorent-Martinez, Eulogio J.; Abdullah, Hassan H.; Ak, Gunes; Senkardes, Ismail; Chiavaroli, Annalisa; Menghini, Luigi; Recinella, Lucia; Brunetti, Luigi; Leone, Sheila; Orlando, Giustino; Ferrante, ClaudioAnacamptis pyramidalis (L.) Rich. forms part of the Orchidaceae family that is highly valued for its horticultural as well as therapeutic benefits. The present study set out to investigate the inhibitory activity of A. pyramidalis tubers against key biological targets for the management of type 2 diabetes, Alzheimer disease, and skin hyperpigmentation. In addition, the antioxidant potential of the extracts was also assessed using multiple methods. The detailed phytochemical profiles of the extracts were determined using high-performance liquid chromatography. Based on qualitative phytochemical fingerprint, a network pharmacology analysis was conducted as well. Parishin was identified from the water extract only, whereas gastrodin and caffeic acid derivatives were present in the methanol extract. The methanol extract exhibited high inhibitory activity against tyrosinase (69.69 mg kojic acid equivalent/g extract), alpha -amylase (15.76 mg acarbose equivalent/g extract), and alpha -glucosidase (20.07 mg acarbose equivalent/g extract). Similarly, the methanol extract showed highest antioxidant potential (22.12, 44.23, 45.56, and 29.38 mg Trolox equivalent/g extract, for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2 ' -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), CUPric Reducing Antioxidant Capacity (CUPRAC), and Ferric Reducing Antioxidant Power (FRAP) assays, respectively). Finally, the results of network pharmacology analysis, besides corroborating traditional uses of plant extracts in the management of cold and flu, confirmed a direct involvement of identified phytochemicals in the observed enzyme inhibitory effects, especially against tyrosinase, alpha -amylase, and alpha -glucosidase. Furthermore, based on the results of both colorimetric assays and network pharmacology analysis related to the activity of A. pyramidalis extracts and identified phytocompounds on enzymes involved in type 2 diabetes, a docking study was conducted in order to investigate the putative interactions of oxo-dihydroxy octadecenoic acid trihydroxy octadecenoic acid against aldose reductase, peroxisome proliferator-activated receptor (PPAR)-alpha, dipeptidyl peptidase (DPP)-IV, and alpha -glucosidase. Docking analysis suggested the inhibitory activity of these compounds against the aforementioned enzymes, with a better inhibitory profile shown by oxo-dihydroxy octadecenoic acid. Overall, the present findings supported the rationale for the use of A. pyramidalis as source of bioactive metabolites and highlight, today more than ever, for the strong necessity of linkage strategy between wild resource valorization and conservation policy.Publication Metadata only Metabolomics-based profiling with chemometric approach to delineate the bio-pharmaceutical properties of fruit extracts from Ligustrum vulgare L(ELSEVIER, 2019) ŞENKARDEŞ, İSMAİL; Rocchetti, Gabriele; Senizza, Biancamaria; Zengin, Gokhan; Senkardes, Ismail; Sadeer, Nabeelah Bibi; Mahomoodally, Mohamad Fawzi; Lucini, LuigiLigustrum vulgare L. (Family: Oleaceae) is an evergreen shrub native to North-west Africa, Europe and Western Asia. In the present work, infusion (water), ethyl acetate, methanol and n-hexane extracts of the fruits of L. vulgare L. were studied for their in vitro antioxidant and enzyme inhibitory properties along with their untargeted polyphenolic profile (using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry approach). Untargeted metabolomics coupled to multivariate statistics showed that Ligustrum fruit extracts were very abundant in polyphenols, such as flavonoids, tyrosols and lignans, with ethyl acetate allowing the highest recovery among the extraction solvents tested. The methanolic extract inhibited the highest acetylcholinesterase (AChE), tyrosinase and glucosidase activity (4.23 mg galatamine equivalent (GALAE)/g, 132.85 mg kojic acid equivalent (KAE)/g and 1.94 mmol acarbose equivalent (ACAE)/g respectively) while the ethyl acetate extract inhibited the highest butyrylcholinesterase (BChE) and amylase activity (4.92 mg GALAE/g and 0.73 mmol ACAE/g, respectively). However, the infusion showed the weakest activity against all the enzymes studied. However, the extract showed highest antioxidant property in'the antioxidant assays (except for phosphomolybdenum). The present paper is the first comprehensive report on L. vulgare fruits and thus our findings are of considerable interest to design new bioproducts for the pharmaceutical and cosmeceutical industry in the future.Publication Open Access The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. plicatum(UNIV SAO PAULO, CONJUNTO QUIMICAS, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Gezmis, Tugba; Cam, Muhammet Emin; Taskin, Duygu; Celik, Berna Ozbek; Senkardes, Ismail; Suzgec-Selcuk, SevdaIn Turkey, Helichrysum genus is represented by 26 taxa belonging to 20 species in Turkish flora of which 14 ones arc endemic to Turkey. The aerial parts of Helichrysum plicatum subsp. plicatum are used kidney stones, kidney and stomach ailments. The extraction procedures and solvents are important step in processing of bioactive constituents from the plant materials. Therefore, the aim of this study was to evaluate in vitro antioxidant, antimicrobial, anti-urease, anticholinesterase and in vivo anti-inflammatory activities of Helichrysum plicatum subsp. plicatum different extracts. In addition, the phenolic characterization of the Soxhlet and maceration methanol extracts which showed significant antioxidant, anti-urease, antimicrobial, anti-inflammatmy and anticholinesterase activities were performed by HPLC-DAD and LC-MS/MS. In the present study, the Soxhlet methanol extract exhibited strong antioxidant, antimicrobial and anticholinestemse activities than other extracts. The maceration methanol extract showed the strongest anti-urease activity. The Soxhlet methanol and maceration methanol extracts showed in vivo anti-inflammatory activities very close to each other. As a result of this study, chlorogenic acid, dicaffeoylquinic acid, luteolin, luteolin-7-O-glucoside, naringenin-O-hexoside and isoquercitrin compounds were analysed in plant. Therefore, it is thought that methanol extracts can be used as a natural source in the future for food and pharmaceutical industries.