Person: ŞENKARDEŞ, İSMAİL
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ŞENKARDEŞ
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İSMAİL
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Publication Metadata only Chemical composition, antiradical, and enzyme inhibitory potential of essential oil obtained from aerial part of Centaurea pterocaula Trautv(TAYLOR & FRANCIS INC, 2021) ŞEN, ALİ; Sen, Ali; Kurkcuoglu, Mine; Yildirim, Aybeniz; Senkardes, Ismail; Bitis, Leyla; Baser, Kemal Husnu CanThe chemical composition, free radical scavenging, alpha-amylase inhibitory, and 5-lipoxygenase inhibitory activity of the essential oil obtained from the aerial parts of Centaurea pterocaula Trautv. were examined. Twenty-nine compounds corresponding to 93.2% of the oil were identified in the oil of C. pterocaula. Hexadecanoic acid (13.9%), caryophyllene oxide (11.5%), spathulenol (11.4%), (E)-beta-damascenone (5.0%), hexahydrofarnesyl acetone (5.0%), and tetradecanoic acid (4.9%) were the main compounds of the oil. The oil exhibited good anti-inflammatory activity, inhibiting 5-lipoxygenase with an IC50 value of 86.22 mu g/mL. The oil was found to be active against DPPH radical and alpha-amylase with IC50 values of 83.05 and 79.66 mu g/mL respectively, while it showed moderate activity against ABTS radicals with IC50 value of 360.9 mu g/mL. To the best of our knowledge, this is first report on the chemical composition of C. pterocaula essential oil from Turkey; as well as on the biological activity of its essential oil.Publication Metadata only Influence of different extraction techniques on the chemical profile and biological properties of Anthemis cotula L.: Multifunctional aspects for potential pharmaceutical applications(ELSEVIER SCIENCE BV, 2019) DOĞAN, AHMET; Sut, Stefania; Dall'Acqua, Stefano; Zengin, Gokhan; Senkardes, Ismail; Bulut, Gizem; Cvetanovic, Aleksandra; Stupar, Alena; Mandic, Anamarija; Picot-Allain, Carene; Dogan, Ahmet; Sinan, Kouadio Ibrahime; Mahomoodally, FawziThe phytochemical composition of different extracts obtained from stinking chamomile (Anthemis cotula L) was investigated. Ethanol was used as solvent and accelerated solvent extraction (ASE), microwave assisted extraction (MAE), maceration, soxhlet extraction (SE), and ultrasound assisted extraction (UAE) were applied on plant material. Comparison of the phytochemical contents, antioxidant, and enzyme inhibitory properties were performed. The most abundant sesquiterpene in the extracts was anthecotuloide, while the most present phenolics were caffeoyl quinic acid, quercetin, and kaempferol derivatives. UAE extract was the most efficient in the extraction of sesquiterpenoids and polyphenols. Considering the assays on antioxidant activity and enzyme inhibition, ASE extract showed highest phenolic content (62.92 mg gallic acid equivalent/g extract). Likewise, this extract showed highest radical scavenging (103.44 mg trolox equivalent [TE]/g extract and 155.70 mg TE/g extract, for DPPH and ABTS assays respectively) and reducing power potential (435.32 and 317.89 mg TE/g extract, for CUPRAC and FRAP assays, respectively). The different extracts showed similar results in the enzyme inhibition assays suggesting that the extraction methods used have no effect on observed enzyme activities. Novelty of our findings are the inhibitory action of the ethanol extract of A. cotula aerial parts on key enzymes associated with Alzheimer's disease (acetyl cholinesterase, butyryl cholinesterase), type 2 diabetes (alpha-amylase, alpha-glucosidase), and skin hyperpigmentation disorders (tyrosinase). Data collected from the present work further appraises the multiple potential biological properties of stinking chamomile suggesting the need for further investigation on its constituents. (C) 2019 Elsevier B.V. All rights reserved.Publication Metadata only Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial(BENTHAM SCIENCE PUBL, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Akkilic, Yeliz; Celik, Berna Ozbek; Senkardes, Ismail; Ozakpinar, Ozlem B.Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant's different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and tlavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using mnicrodilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (('UPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicatis strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.Publication Metadata only UHPLC-LTQ OrbiTrap MS analysis and biological properties of Origanum vulgare subsp. viridulum obtained by different extraction methods(ELSEVIER, 2020) ŞENKARDEŞ, İSMAİL; Zengin, Gokhan; Cvetanovic, Aleksandra; Gasic, Uros; Dragicevic, Milan; Stupar, Alena; Uysal, Ahmet; Senkardes, Ismail; Sinan, Kouadio Ibrahime; Picot-Allain, Marie Carene Nancy; Ak, Gunes; Mahomoodally, Mohamad FawziThe growing awareness on implications related to the use of harsh chemicals and non-sustainable extraction methods of bioactive compounds from plants is causing a paradigm shift towards a more eco-friendly approach. The present study sets out to investigate the possible variation in the antioxidant, mutagenic/antimutagenic, antimicrobial, and enzyme inhibitory properties of Origanum vulgare subsp. viridulum extracts obtained using conventional, namely, maceration and Soxhlet extraction, and non-conventional, namely, ultrasound-assisted (UAE), microwave-assisted (MAE), and accelerated solvent extraction (ASE), techniques. Also, chemical characterization of these extracts were performed via ultrahigh-performance liquid chromatography and mass spectrometry (UHPLC-LTQ OrbiTrap MS) technique. A total of 58 polyphenols were identified in the tested extracts. Highest phenolic content was obtained from ASE (186.63 mg gallic acid equivalent [GAE]/g) and MAE (170.90 mg GAE/g) extracts. Maceration extract (18.89 mg rutin equivalent [RE]/g) showed highest flavonoid content. ASE extract showed highest activity in the DPPH (359.45 mg Trolox equivalent [TE]/g), ABTS (679.63 mg TE/g), FRAP (1433.94 mg TE/g), and CUPRAC (1685.72 mg TE/g) assays. In terms of enzyme inhibition, the UAE extract showed highest activity against acetyl cholinesterase (4.55 mg galantamine equivalent [GALAE]/g), butyryl cholinesterase (6.12 mg GALAE/g), and tyrosinase (173.87 mg kojic acid equivalent [KAE]/g) compared with the other extracts. The extracts exhibited significant antimutagenic and antimicrobial properties. Data collected from this study support the use of ASE and UAE in the extraction of bioactive compounds from Origanum vulgare subsp. viridulum aerial parts.