Person: ULUCAN, KORKUT
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ULUCAN
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KORKUT
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Publication Open Access Determination of HIF1-Ars11549465 Polymorphism in Elite_x000D_ Skiers(2019-08-31) ULUCAN, KORKUT; Ömer KAYNAR;M. Fatih BİLİCİ;Canan SERCAN;Korkut ULUCANStudies in sports genetics have increased nowadays and the identification of genotype and allelic distributions of candidategenes in different athletes has provided important information to sports scientists. One of the candidate genes studied in sportsgenetics is the hypoxia-inducible factor 1-alpha (HIF1A) gene, which is associated with the endurance phenotype. In our study,we aimed to determine the genotypic and allelic distribution of HIF1A rs11549465 polymorphism in Turkish Elite cross-countryathletes. 34 (23 male, 11 female) athletes were included in the study, and 1 cc of blood samples taken for routine analysis afterthe approval of the ethics committee was used for DNA isolation. The commercial kit was used for DNA isolation andgenotyping was performed by Real-time PCR method. The number and percentage of CC, CT and TT genotypes of the HIF1Ars11549465 polymorphism were 24 (72.72%), 8 (24.24%) and 1 (3.03), respectively. In male skiers CC, CT and TT genotypenumbers and percentages were determined as 17 (55.51%), 4 (12.12%) and 1 (3.03%) respectively. In female, CC and TTgenotypes were determined as 7 (21.21%) and 4 (12.12%). We detected no TT genotype in female athletes. When we look at allelnumbers, C allele was determined as 56 (84.84%) and T allele was determined as 10 (15.15%). This is the first study on HIF1Ars11549465 polymorphism on Turkish elite ski cross-country athletes. The higher prevalence of CC genotype and C allele in theathlete group is similar to the previous studies on different groups of athletes. We believe that this study will be an importantreference for further studies.Publication Metadata only MMP3 (Rs679620) ve VDR (Rs731236) gen polimorfizmlerinin, periodontal olarak sağlikli erkek bireylerde, diş çürük lezyonu oluşumundaki etkisinin değerlendirilmesi: pilot çalışma(2023-01-11) ÖZMEN, SEDA; YILMAZ ATALI, PINAR; AĞRALI, ÖMER BİRKAN; TACAL ASLAN, BESTE; ULUCAN, KORKUT; Özmen S., Yılmaz Atalı P., Ağralı Ö. B., Tacal Aslan B., Yılmaz Ö. Ö., Polat T., Ulucan K.Amaç:Çürük oluşumu pek çok faktörün etkilediği bir süreçtir. Yapılan çalışmalar genetik faktörlerin de çürük oluşumunda rol oynadığını göstermiştir. Çalışmamızda, östrojen hormon mekanizması ve mensturasyon döngüsünün gen ekspresyon sürecini değiştirmesi sebebiyle, erkek bireylerde diş mineralizasyon sürecinde rol oynayanMMP3veVDRgenlerinde oluşan polimorfizmlerin çürük oluşumuna olan etkisinin incelenmesi amaçlanmıştır.Gereç ve Yöntem:Fakülte hastanesine başvuran 20-44 yaş aralığındaki erkek bireylerde rutin ağız içi muayenesi sonrasında; çürük, eksik ve restorasyonlu dişler (DMFT) indeksine göre tanı konulmuştur. Deney grubunu, ‘yüksek çürük risk’ (DMFT ≥ 14, n=28); kontrol grubunun ise ‘çürük lezyonu bulunmayan’ (DMFT=0, n=28) kişiler oluşturmuştur. Detaylı anamnez alınan katılımcılardan plak indeksi, sondalamada kanama ölçümü yapılmıştır.Katılımcılardan alınan kan örneklerinden DNA izolasyonları sonrasında,MMP3(rs679620)veVDR(rs731236)genotiplemesi Real-time PZR tekniği kullanılarak belirlenmiştir.Elde edilen sonuçlar istatistiksel yöntemler kullanılarakanlamlılık düzeyi p<0,05’te değerlendirilmiştir.Bulgular:Çalışmamıza katılan bireylerde sondalamada kanama derecesi %10’un altında ve DMFT=0 olan düşük risk grubundaki (kontrol) ve de sondalamada kanama derecesi %10’un altında olan yüksek risk grubundaki bireylerin (deney)MMP3veVDRpolimorfizmleri açısından kıyaslaması yapılmış ve gruplara göreMMP3dağılımları arasında istatistiksel olarak anlamlı farlılık bulunmuştur (p<0,001). Gruplara göre VDR dağılımları arasında istatistiksel olarak anlamlı farklılık bulunmamıştır (p=0,659)Sonuç:Çalışmamızın sınırları dahilinde MMP3(rs679620) gen polimorfizminin çürük oluşum üzerinde etkili;VDR(rs731236) polimorfizminin ise etkisiz olduğu sonucuna ulaşılmıştır. Katılımcı sayısının arttırılarak ileri çalışmaların yapılması gerektiği düşünülmektedir.Anahtar Kelimeler:Çürük risk değerlendirmesi, gen-çevre etkileşimi, MMP3, polimorfizm, VDR.Publication Open Access Prevalence of alpha actinin-3 gene (ACTN3) R577X and angiotensin converting enzyme (ACE) insertion / deletion gene polymorphisms in national and amateur Turkish athletes(C M B ASSOC, 2018-04-30) ULUCAN, KORKUT; Eroglu, Onur; Zileli, Raif; Nalbant, M. Ali; Ulucan, KorkutStudies to date showed the importance of alpha- actinin-3 (ACTN3) R577X and angiotensin converting enzyme (ACE) ID polymorphisms on determining athletic performance. Therefore, in this study, we aimed to examine polymorphisms given to Turkish athletes and compare them with sedentary individuals. Genomic DNAs were extracted from peripheral blood by using commercially available DNA isolation kit (Macherey-Nagel, NucleoSpin (R), Germany). For this study, a total of 84 volunteers (23 national athletes, 27 amateur athletes and 34 sedentary controls) was recruited. ACE ID genotypes were determined by conventional polymerase chain reaction, and ACTN3 R577X polymorphisms by polymerase chain reaction- restriction fragment length polymorphism methodology. In ACTN3 R577X polymorphism, RX was the dominating genotype, and we detected no RR genotype in national athletes. (no RR genotype was detected in national athletes) X allele is more frequent in national athletes and R allele was more frequent in both amateur athletes and control group. II genotype was more frequent in national athletes and in control group, whereas DD genotype was more frequent in amateur athletes for ACE ID polymorphism. When we consider alleles, D allele was found more frequently in amateur athletes and control group whereas I allele was more frequent in national athletes in ACE ID polymorphism. For ACTN3, X allele was superior to R allele. ACTN3 R577X and ACE ID polymorphisms were important biomarkers in determining athletic performance. However, our results in Turkish athletes suggest that ACE D allele and ACTN3 X alleles may be beneficial to athletes potentially, regardless of the distance they perform.Publication Metadata only Can Neurogulin 1 be an Important Biomarker for Creativity in Sports?(ANNALS APPLIED SPORT SCIENCE, 2016) ULUCAN, KORKUT; Arslan, Kadir Sinan; Akpunar, Fatmanur; Ulucan, KorkutPublication Metadata only Literature Review of Turkish Sportsmen in Terms of ACTN3 R577X Polymorphism(AVES PRESS LTD, 2016) ULUCAN, KORKUT; Ulucan, KorkutThe effect of genes on individual athletic performance is accepted; however, the extent of their effects remains elusive. To date, many studies on different genomic regions that are considered to have an effect on human athletic performance and sportsmen in different types of sports, such as football, basketball, judo, and wrestling, have been conducted. However, the number of these studies with Turkish players as subjects is not adequate. This literature review aimed to collate the results of alpha-actinin-3 (ACTN3) R577X polymorphisms of Turkish athletes after an extensive literature survey. After the survey, we could reach 518 players, including football, basketball, and wrestling players. Of these, 234 were RR (45%), 207 (40%) were RX, and the rest were XX. Power and sprinter R allele counts were 665 (64%), and the endurance allele count was 371 (36%). According to the obtained data, the number and percentage of RR+RX genotypes and R allele were higher than those of XX genotype and X allele. We consider that this first report will be an informative source of information for Turkish players in the terms of ACTN3 R577X polymorphism and Turkish sportsman and will be a valuable guide for new researches.Publication Open Access Investigation of the association between mitochondrial DNA and p53 gene mutations in transitional cell carcinoma of the bladder(SPANDIDOS PUBL LTD, 2016-10) GÜNEY, AHMET İLTER; Avcilar, Tuba; Kirac, Deniz; Ergec, Deniz; Koc, Gulsah; Ulucan, Korkut; Kaya, Zehra; Kaspar, Elif Cigdem; Turkeri, Levent; Guney, Ahmet IlterBladder carcinoma is the most common malignancy of the urinary tract. The major aim of the present study is to investigate the association between mitochondrial DNA (mtDNA) and p53 gene mutations in bladder carcinoma. A total of 30 patients with transitional cell carcinoma and 27 controls were recruited for the study. Bladder cancer tissues were obtained by radical cystectomy or transurethral resection. Genomic DNA was extracted from peripheral blood. mtDNA and p53 genes were amplified by polymerase chain reaction and sequenced directly. A total of 37 polymorphisms were identified, among which, 2 mutations were significant in the patient group, and 1 mutation was significant in the control group. Additionally, 5 different moderate positive correlations between mtDNA mutations and 3 different positive correlations between p53 gene and mtDNA mutations were detected. The high incidence of mtDNA and p53 gene mutations in bladder cancer suggests that these genes could be important in carcinogenesis.Publication Metadata only Kayakta başarılı olan oğlağo ailesine mensup bireylerin genetik özelliklerinin incelenmesi(2021-11-30) TACAL ASLAN, BESTE; ULUCAN, KORKUT; KAYNAR Ö., KOÇ H., Ferhatoğlu Y., POLAT T., TACAL ASLAN B., ULUCAN K.Publication Open Access Exome-wide association study of competitive performance in elite athletes(2023-03-01) ULUCAN, KORKUT; Bulgay C., Kasakolu A., Kazan H. H., Mijaica R., ZORBA E., Akman O., Bayraktar I., Ekmekci R., KONCAGÜL S., ULUCAN K., et al.The aim of the study was to identify genetic variants associated with personal best scores in Turkish track and field athletes and to compare allelic frequencies between sprint/power and endurance athletes and controls using a whole-exome sequencing (WES) approach, followed by replication studies in independent cohorts. The discovery phase involved 60 elite Turkish athletes (31 sprint/power and 29 endurance) and 20 ethnically matched controls. The replication phase involved 1132 individuals (115 elite Russian sprinters, 373 elite Russian endurance athletes (of which 75 athletes were with VO2max measurements), 209 controls, 148 Russian and 287 Finnish individuals with muscle fiber composition and cross-sectional area (CSA) data). None of the single nucleotide polymorphisms (SNPs) reached an exome-wide significance level (p < 2.3 × 10−7) in genotype–phenotype and case–control studies of Turkish athletes. However, of the 53 nominally (p < 0.05) associated SNPs, four functional variants were replicated. The SIRT1 rs41299232 G allele was significantly over-represented in Turkish (p = 0.047) and Russian (p = 0.018) endurance athletes compared to sprint/power athletes and was associated with increased VO2max (p = 0.037) and a greater proportion of slow-twitch muscle fibers (p = 0.035). The NUP210 rs2280084 A allele was significantly over-represented in Turkish (p = 0.044) and Russian (p = 0.012) endurance athletes compared to sprint/power athletes. The TRPM2 rs1785440 G allele was significantly over-represented in Turkish endurance athletes compared to sprint/power athletes (p = 0.034) and was associated with increased VO2max (p = 0.008). The AGRN rs4074992 C allele was significantly over-represented in Turkish sprint/power athletes compared to endurance athletes (p = 0.037) and was associated with a greater CSA of fast-twitch muscle fibers (p = 0.024). In conclusion, we present the first WES study of athletes showing that this approach can be used to identify novel genetic markers associated with exercise- and sport-related phenotypes.Publication Open Access FUTBOLCULARDA SLC6A4 PROMOTÖR POLİMORFİZMİNİN DAĞILIMI(2021-11-26) TACAL ASLAN, BESTE; Başak Funda EKEN;Tolga POLAT;Canan SERCAN DOĞAN;Beste Tacal ASLAN;Şehkar OKTAY;Korkut ULUCANİnsan psikolojisini etkileyen ve en önemli bir biyobelirteç olan serotonin, sporcunun atletik performansını da olumsuz yönde etkileyebilmektedir. Çalışmamızın amacı, sağlıklı profesyonel futbolcularda anksiyete ile bağlantılı SLC6A4 geni promotör bölgesinde “S” ve “L” allel dağılımının incelenmesidir. Çalışmamıza aktif olarak haftada en az 4 gün antrenman programı uygulayan 15-29 yaş arası 19 profesyonel futbolcu ve herhangi bir egzersiz programı uygulamayan (kontrol grubu) 45 birey katıldı. Gerekli etik kurul izinleri alındıktan sonra çalışma protokolunun sonuç ve çıktılarını anlatan onam formları katılımcılara imzalatıldı. Çalışmamıza katılan bireylerden daha sonra ağız içi epitel hücreleri alınarak DNA izolasyonu gerçekleştirildi. Tüm polimorfizmlerin genotiplemesi, polimeraz zincir reaksiyonu (PZR) metodu kullanılarak belirlendi. Çalışmamıza katılan futbolcularda SLC6A4 geninin LL, LS, SS genotiplerinin sayı ve yüzdeleri sırasıyla 11(%57,8), 4(%21,1), 4(%21,1) olarak belirlenmiştir. Çalışma kohortumuzda L alleli 26 (%68,4), S alleli ise 12 (%31,6) oranında gözlemlenmiştir. Çalışma grubumuzda SLC6A4 geninin promotör bölgesi incelendiğinde, LL genotipi baskın olarak bulunurken, L alleli de S alleline göre daha yüksek oranda bulunmuştur. Kontrol grubunda ise aynı genotip sayı ve yüzdeleri sırası ile 12 (%26,7), 24 (%53,3) ve 9 (%20) olarak belirlenmiştir. Kontrol grubundaki allelik dağılımı ise L alleli 48(%53,3), S alleli 42 (%46,7) olarak gözlenmiştir. Sporcu grubu ile kontrol grubu arasında genotip dağılımlarında (p=0,0001) bulunurken, allelik dağılımlarında (p=0,0425) bulunarak her iki grup arasında da istatiksel açıdan anlamlı farklılık saptanmıştır. Sonuç olarak çalışmamızda SLC6A4 promotör polimorfizmlerinin belirlenmesinin, sporcularda oluşan anksiyete ve bilişsel kaygı düzeyinin erken dönemde önlenmesine yardımcı olabileceği sonucuna varılmıştır.Publication Open Access Distribution of Methylenetetrahydrofolate Reductase rs1801133 Polymorphism in a Turkish Professional Cyclist Cohort(ANNALS APPLIED SPORT SCIENCE, 2018-08-01) ULUCAN, KORKUT; Yuksel, Ipek; Sercan, Canan; Kapici, Sezgin; Biyik, Betul; Kaman, Tugba; Ulucan, KorkutBackground. The determination of the genetic endowment of athletic performance in sports is an important step in developing personal training sessions or nutritional supplements for success in sports. Information about the genetic parameters responsible for these metabolisms will help sport's scientist to develop new insights for better performance. Muscle metabolism is one of the key points in better personal athletic performance. Objectives. The aim of this study is to analyze the distribution of the methylenetetrahydrofolate reductase enzyme (MTHFR) rs1801133 (C677T) genotype and allele distribution in a Turkish professional cyclist cohort. Methods. There were 25 Turkish cyclists enrolled in the study. Peripheral blood used for DNA isolation and the conventional polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology were used for genotyping. Results. There were 14 (56%), 10 (40%) and 1 (4%) cyclist who had CC, CT, and TT genotypes, respectively. C allele was counted as 38 (76%), and T alleles as 12 (24%). 9 (50%) of the male cyclist had CC, 8 (44.4%) had CT and only 1 had TT (5.6%) genotypes. C allele was counted as 26 (72.2%), and the T allele as 10 (27.8%) in the male cyclists. In the females, the respective genotypes for CC and CT were 5 (71.4%) and 2 (28.6%). C allele was counted as 12 (85.7%) and T allele as 2 (14.3%). Conclusion. In our cohort, both of the two genders, the CC genotype and C allele were found to be higher when compared to the other genotypes and T allele. Larger prospective studies focusing on the influence of MTHFR rs1801133 polymorphism in athletic performance are required for confirmation of our findings.