Person: ÖZBEYLİ, DİLEK
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ÖZBEYLİ
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DİLEK
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Publication Metadata only Protective effect of low dose caffeine on psychological stress and cognitive function(PERGAMON-ELSEVIER SCIENCE LTD, 2017) AKAKIN, DİLEK; Cakir, Ozgur Kasimay; Ellek, Nurfitnat; Salehin, Nabila; Hamamci, Rabia; Keles, Hulya; Kayali, Damla Gokceoglu; Akakin, Dilek; Yuksel, Meral; Ozbeyli, DilekIntroduction: Caffeine is an adrenergic antagonist that enhances neuronal activity. Psychological stress depresses cognitive function. Aim: To investigate the effects of acute and chronic low dose caffeine on anxiety-like behavior and cognitive functions of acute or chronic psychological stressed rats. Material-method: Acute or chronic caffeine (3 mg/kg) was administered to male Sprague Dawley rats (200-250 g, n = 42) before acute (cat odor) and chronic variable psychological stress (restraint overcrowding stress, elevated plus maze, cat odor, forced swimming) induction. Anxiety and cognitive functions were evaluated byhole-board and object recognition tests. The brain glutathione and malondialdehyde assays, myeloperoxidase, nitric oxide (NO), superoxide dismutase (SOD), luminol and lucigenin activity and histological examination were done. ANOVA and Student's t-test were used for statistical analysis. Results: The depressed cognitive function with chronic stress exposure and the increased anxiety-like behavior with both stress inductions were improved via both caffeine applications (p < 0.05-0.001). Both caffeine pretreatments in chronic stressed rats, and chronic caffeine in acute stressed ones reduced the elevated myeloperoxidase activities (p < 0.05-0.01). The increased malondialdehyde, lucigenin and NO levels with acute stress were inhibited with chronic caffeine (p < 0.05-0.01), malondialdehyde and NO levels were declined by acute caffeine (p < 0.001). Acute caffeine decreased SOD activity (p < 0.01) and improved glutathione (p < 0.01) and luminol levels (p < 0.05). The induced histological damage with both stress exposures was ameliorated with chronic caffeine. Conclusion: The increased anxiety-like behavior and depleted cognitive functions under stress conditions were improved with both acute and predominantly chronic caffeine pretreatments by decreasing oxidative damage parameters. (C) 2016 Elsevier Inc. All rights reserved.Publication Metadata only The protective role of UMCA on bleomycin-induced lung fibrosis in rats(2022-11-05) AYDEMİR, SEZGİN; ÖZKAN YENAL, NAZİYE; GÜÇLÜ, HÜLYA; YÜKSEL, MERAL; ÖZBEYLİ, DİLEK; AYDEMİR S., ÖZKAN YENAL N., GÜÇLÜ H., YÜKSEL M., ÖZBEYLİ D., ERDOĞAN N.Bleomycin (BLM), an antibiotic drug, is used for the treatment of cancers like Hodgkin’s lymphoma, Kaposi’s sarcoma and cervical cancer. The most common adverse effects occurred during BLM treatment is lung toxicity, which manifest as fibrosis. UMCA® (Pelargonium sidoides root extract) is a commercial product to treat acute and chronic upper respiratory tract infections. UMCA® has antiviral, antibacterial, immunomodulatory and cytoprotective properties, which is related to its polyphenol, coumarin, anthocyanidin and flavonoid contents. In the present study, we aimed to investigate the possible protective effects of UMCA® on BLM induced lung fibrosis in rats. Wistar albino rats was included in this study and randomly divided into 4 groups (Control, BLM, BLM+UMCA and UMCA). The control group received physiological saline. UMCA® was orally applied at a dose of 200 mg/kg/day and single intratracheal administration of BLM was conducted at a dose of 10 mg/kg. All animals were decapitated after 10 days and lung tissues was removed for the biochemical and histopathological examinations. Malondialdehyde (MDA) and glutathione (GSH) levels and Myeloperoxidase (MPO) activity were determined. Chemiluminescence (CL) method using luminol and lucigenin probes, additional nitric oxide and peroxynitrite measurements were applied. Histopathological observations were analyzed with H&E and Gomori's one-step trichrome staining. Collagen contents of lung tissue were also determined with a spectrophotometric method in paraffin-embedded tissues. BLM elevated MDA levels and MPO activity and depleted GSH levels in the lung tissues (p<0.001). CL measurement levels were also increased in BLM group respect to the control. Contrary to this, UMCA® treatment reversed these effects, significantly (p<0.001). BLM caused alveolar structural disturbance and inflammatory cell infiltrations and collagen content was also significantly increased in BLM group compared to the control (65.7±10.6, p<0.001). UMCA® administration reduced degenerations and decreased collagen content in the lung tissue. In conclusion, UMCA® has an antioxidant and protective effects on BLM induced lung fibrosis in rats.Publication Metadata only Hepatoprotective effects of parsley (Petroselinum Crispum) extract in rats with bile duct ligation(2022-01-01) ÖZBEYLİ, DİLEK; ERCAN, FERİHA; YÜKSEL, MERAL; Ede S., ÖZBEYLİ D., Erdoğan Ö., Çevik Ö., Kanpalta F., ERCAN F., YANARDAĞ R., SAÇAN Ö., ERTİK O., YÜKSEL M., et al.© 2022 Pan-Arab Association of GastroenterologyBackground and study aims: This study aimed to investigate the possible protective effects of parsley extract (Petroselinum Crispum; PC) against oxidative liver damage caused by bile obstruction in rats. Material and methods: Bile duct ligation (BDL) method was used to induce liver injury in rats. The rats were divided into the three groups each consisting of 8 rats; Sham-operated control (C), bile duct ligated + saline treated (BDL), and BDL + PC treated groups. PC extract was given at a dose of 2 g/kg orally for 28 days. Aspartate amino transferase (AST), alanin amino transferase (ALT), and bilirubin levels were analyzed in sera. In order to determine free radicals in liver injury, luminol and lucigenin chemiluminescence tests used. Oxidative stress was evaluated through superoxide dismutase, glutathione, malondialdehyde, Na+/K+-ATPase and 8-hydroxy guanosine levels. Furthermore, inflammation marker myeloperoxidase, apoptosis marker caspase-3, and fibrosis markers TGF- β and hydoxyproline were investigated. The liver tissues were also examined for histological evaluations. Results: While PC treatment decreased AST and ALT levels which increased with BDL, oxidant damage parameters also decreased with this treatment. Conclusion: The present study, which is the first research for PC extract on cholestasis induced liver damage, demonstrated that PC extract could be a potential therapeutic agent against liver fibrosis and need further studies.Publication Metadata only Protective effects of different exercise modalities in an Alzheimer's disease-like model(ELSEVIER SCIENCE BV, 2017) YILMAZ, BETÜL; Ozbeyli, Dilek; Sari, Gulce; Ozkan, Naziye; Karademir, Betul; Yuksel, Meral; Kaya, Ozlem Tugce Cilingir; Cakir, Ozgur KasimayOur aim was to investigate the probable protective effects of aerobic, resistance and combined exercise methods on ovariectomy and D-galactose induced Alzheimer's Disease (AD)-like model. D-galactose (100 mg/kg) or saline were administered intraperitoneally for 6 weeks to ovariectomized or sham-operated rats (n = 8/group). Aerobic (AE), resistance (RE) and combined exercises (CE) (aerobic + resistance) were performed for 3 times a week for 6 weeks. Anxiety level and cognitive functions were evaluated via hole-board and object recognition tests. Brain myeloperoxidase, malondialdehyde, nitric oxide activity, lucigenin-enhanced chemiluminescence, glutathione and serum insulin like growth factor-I (IGF-I) assays were done. Hippocampal mRNA levels of nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and amyloid precursor protein 695 (APP695) were measured. Amyloid Beta (A beta), NGF, BDNF, IGF-I immunoreactive neurons were evaluated. Freezing time were increased in AD like model and decreased back with AE (p <0.05). Deteriorated working memory in AD-like model was improved with all exercise types (p < 0.05-0.001). Reduced glutathione levels in AD-like model were increased and increased malondialdehyde levels were reduced and serum IGF-I levels were increased by all exercises (p < 0.05-0.001). Increased APP mRNA levels in AD-like model were decreased via CE (p < 0.05). Elevated AP scores in AD-like model were decreased by RE and CE (p < 0.01) in hippocampus and by all exercise types in cortex (p < 0.05-0.01). Decreased cortical NGF immunocytochemical scores of AD-like model were increased by CE (p < 0.05). Different exercise models may have protective effects in development stage of AD via reducing oxidative stress and A beta scores, and by improving antioxidant system and brain plasticity. (C) 2017 Elsevier B.V. All rights reserved.Publication Open Access Astaxanthin alleviates oxidative damage in acute pancreatitis via direct antioxidant mechanisms(2020-10-30) ÖZBEYLİ, DİLEK; Department of Medical Pathological Techniques, Marmara University, Vocational School of Health Services, Istanbul, Turkey; Ozbeyli, Dilek; Gurler, Esra Bihter; Department of Physiology, Istanbul Atlas University School of Medicine, Istanbul, Turkey; Buzcu, Hulya; Department of Physiology, University of Health Sciences School of Medicine, Istanbul, Turkey; Cilingir-Kaya, Ozlem Tugce; Department of Histology and Embryology, Marmara University School of Medicine, Istanbul, Turkey; Cam, Muhammet Emin; Department of Pharmacology, Marmara University School of Pharmacy, İstanbul, Turkey; University College London, Department of Mechanical Engineering,Torrington Place, London, UK; Yuksel, Meral; Department of Medical Laboratory Techniques, Marmara University, Vocational School of Health Services, Istanbul, TurkeyPublication Metadata only NESFATIN-1 PROTECTS FROM ACUTE PANCREATITIS: ROLE OF MELANOCORTIN RECEPTORS(POLISH PHYSIOLOGICAL SOC, 2019) ÖZBEYLİ, DİLEK; Buzcu, H.; Ozbevli, D.; Yuksel, M.; Kava, O. T. Cilingir; Cakir, O. KasimavNesfatin-1, a recently discovered peptide, was shown to have anti-inflammatory effects. Acute pancreatitis (AP) is a life-threatening condition caused by various reasons. Although the etiology of AP is well-known, its pathogenesis is not clear. The aim of this study is to investigate the possible anti-inflammatory role of nesfatin-1 and its probable protective underlying mechanisms in an acute pancreatitis model. Caerulein was applied intraperitoneally to induce acute pancreatitis in Sprague-Dawley female rats. Nesfatin-1 was administered 5 minutes before the application of caerulein to determine its potential anti-inflammatory role on AP. Five minutes before nesfatin-1 injection, in order to investigate the underlying mechanism, oxytocin receptor antagonist (atosiban), melanocortin receptor antagonist (HS024), or ghrelin receptor antagonist (cortistatin) were administered. Five minutes after nesfatin-1 administration, two doses of caerulein were applied one hour apart. The rats were sacrified 12 hours after the first caerulein dose for serum and pancreatic tissue sampling. Microscopic damage scoring, malondialdehyde and glutathione levels, myeloperoxidase activity, luminol and lucigenin chemiluminescence levels in pancreatic tissue and amylase, lipase, trypsinogen-2 levels in serum were evaluated. Oxidative damage was decreased with nesfatin-1 treatment in the acute pancreatitis model (P < 0.05 - 0.001). The administration of HS024 reversed the effect of nesfatin-1, via increasing lipase, amylase, trypsinogen-2, malondialdehyde (MDA), myeloperoxidase (MPO) and lucigenin levels (P < 0.05 - 0.01). Atosiban pre-treatment elevated MPO activity, luminol and lucigenin chemiluminescence levels (P < 0.01 - 0.001) and cortistatin increased lucigenin and luminol chemiluminescence (P < 0.05 - 0.01). Although receptor antagonists reversed the effect of nesfatin-1 on related biochemical parameters, no significant difference was found in histological scoring. Our results indicated that nesfatin-1 had an anti-inflammatory effect on acute pancreatitis via mainly effecting melanocortin receptors.Publication Metadata only Protective effect of exercise and sildenafil on acute stress and cognitive function(PERGAMON-ELSEVIER SCIENCE LTD, 2015) AKAKIN, DİLEK; Ozbeyli, Dilek; Gokalp, Ayse Gizem; Koral, Tolga; Ocal, Onur Yuksel; Dogan, Berkay; Akakin, Dilek; Yuksel, Meral; Kasimay, OzgurIntroduction: There are contradictory results about the effects of exercise and sildenafil on cognitive functions. Aim: To investigate the effects of sildenafil pretreatment and chronic exercise on anxiety and cognitive functions. Method: Wistar rats (n = 42) were divided as sedentary and exercise groups. A moderate-intensity swimming exercise was performed for 6 weeks, 5 days/week, 1 h/day. Some of the rats were administered orogastrically with sildenafil (25 mg/kg/day) either acutely or chronically. Exposure to cat odor was used for induction of stress. The level of anxiety was evaluated by elevated plus maze test, while object recognition test was used to determine cognitive functions. Brain tissues were removed for the measurement of myeloperoxidase (MPO), malondialdehyde (MDA), nitric oxide levels, lucigenin-enhanced chemiluminescence, and for histological analysis. Results: Increased MPO and MDA levels in sedentary-stressed rats were decreased with sildenafil applications. Chronic exercise inhibited the increase in MPO levels. Increased nitric oxide and lucigenin chemiluminescence levels in sedentary-stressed rats, were diminished with chronic sildenafil pretreatment. The time spent in the open arms of the plus maze was declined in sedentary-stressed rats, while chronic sildenafil pretreatment increased the time back to that in non-stressed rats. Acute sildenafil application to exercised rats prolonged the time spent in open arms as compared to non-treated exercise group. The time spent with the novel object, which was decreased in sedentary-stressed rats, was increased with sildenafil pretreatment. Our results suggest that sildenafil pretreatment or exercise exerts a protective effect against acute stress and improves cognitive functions by decreasing oxidative damage. (C) 2015 Elsevier Inc All rights reserved.Publication Metadata only Protective effect of alpha-lipoic acid, aerobic or resistance exercise from colitis in second hand smoke exposed young rats(WILEY, 2017) AKAKIN, DİLEK; Ozbeyli, Dilek; Berberoglu, Ayse Cansu; Ozen, Anil; Erkan, Oktay; Basar, Yunus; Sen, Tunahan; Akakin, Dilek; Yuksel, Meral; Cakir, Ozgur KasimayThe role of second hand smoke (SHS) exposure on ulcerative colitis is not known. Our aim was to examine the effects of -lipoic acid (ALA), chronic aerobic (AE) or resistance exercise (RE) on SHS exposed rats with colitis. Sprague-Dawley male rats (150-200g, n=54) were selected for colitis induction. Among the colitis groups, one group was exposed to SHS (6d/wk, 4cigarettes/d) and the other was not. The SHS group was divided into subgroups as follows: sedentary; AE (swimming; 3d/wk); and RE (climbing with weight; 3d/wk). After 5weeks, colitis was induced by intrarectal acetic acid. All groups had subgroups that were given subcutaneously ALA (50mg/kg per day) or vehicle for 3days. Following decapitation, colon tissues were sampled to examine malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminenscence, macroscopic scoring and histologic examination. ANOVA and Student's t-test were used for statistical analysis. The increased macroscopic and microscopic scores, MPO, MDA, luminol and lucigenin measurements in colitis and SHS-colitis groups were decreased via ALA (P<.05-.001). AE declined macroscopic and microscopic scores, MDA, lucigenin compared to colitis and SHS-colitis groups (P<.01-.001). RE reduced microscopic score, MPO, MDA, luminol, lucigenin (P<.05-.001) that were increased with colitis. Decreased GSH levels (P<.01) in the SHS-colitis group approached to control levels when given ALA. According to our results SHS and colitis induction increased inflammatory damage. SHS did not worsen it more than colitis. Our results suggest that ALA, AE or RE might be protective for SHS exposed ulcerative colitis conditions.