Publication: The protective role of UMCA on bleomycin-induced lung fibrosis in rats
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Date
2022-11-05
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Abstract
Bleomycin (BLM), an antibiotic drug, is used for the treatment of cancers like Hodgkin’s lymphoma,
Kaposi’s sarcoma and cervical cancer. The most common adverse effects occurred during BLM
treatment is lung toxicity, which manifest as fibrosis. UMCA® (Pelargonium sidoides root extract)
is a commercial product to treat acute and chronic upper respiratory tract infections. UMCA®
has antiviral, antibacterial, immunomodulatory and cytoprotective properties, which is related to
its polyphenol, coumarin, anthocyanidin and flavonoid contents. In the present study, we aimed
to investigate the possible protective effects of UMCA® on BLM induced lung fibrosis in rats.
Wistar albino rats was included in this study and randomly divided into 4 groups (Control, BLM,
BLM+UMCA and UMCA). The control group received physiological saline. UMCA® was orally
applied at a dose of 200 mg/kg/day and single intratracheal administration of BLM was conducted
at a dose of 10 mg/kg. All animals were decapitated after 10 days and lung tissues was removed
for the biochemical and histopathological examinations. Malondialdehyde (MDA) and glutathione
(GSH) levels and Myeloperoxidase (MPO) activity were determined. Chemiluminescence
(CL) method using luminol and lucigenin probes, additional nitric oxide and peroxynitrite
measurements were applied. Histopathological observations were analyzed with H&E and
Gomori's one-step trichrome staining. Collagen contents of lung tissue were also determined with
a spectrophotometric method in paraffin-embedded tissues. BLM elevated MDA levels and MPO
activity and depleted GSH levels in the lung tissues (p<0.001). CL measurement levels were also
increased in BLM group respect to the control. Contrary to this, UMCA® treatment reversed these
effects, significantly (p<0.001). BLM caused alveolar structural disturbance and inflammatory
cell infiltrations and collagen content was also significantly increased in BLM group compared to
the control (65.7±10.6, p<0.001). UMCA® administration reduced degenerations and decreased
collagen content in the lung tissue. In conclusion, UMCA® has an antioxidant and protective
effects on BLM induced lung fibrosis in rats.
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AYDEMİR S., ÖZKAN YENAL N., GÜÇLÜ H., YÜKSEL M., ÖZBEYLİ D., ERDOĞAN N., \"The protective role of UMCA on bleomycin-induced lung fibrosis in rats\", 11th INTERNATIONAL CONGRESS OF THE TURKISH SOCIETY OF TOXICOLOGY, Antalya, Türkiye, 2 - 05 Kasım 2022