Person: PİNAR, ORKUN
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PİNAR
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ORKUN
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Publication Metadata only Retro-techno-economic evaluation of acetic acid production using cotton stalk as feedstock(SPRINGER, 2018) SAYAR, AHMET ALP; Sayar, Nihat Alpagu; Kazan, Dilek; Pinar, Orkun; Akbulut, Berna Sariyar; Sayar, Ahmet AlpIn value-added chemical industries, use of agricultural wastes as raw materials remains to be a major challenge in commercialization due to lack of competitiveness with respect to petrochemical processes. This work presents the techno-economic analysis of a novel bioprocessing plant converting 356,400MT/year cotton stalks into 147,000MT/year acetic acid. A production scheme integrating lignin separation with the main bioconversion stages has been proposed. Techno-economic assessment was performed through economic feasibility and retro-techno-economic analysis (RTEA) methods. The RTEA method has been extended to include the estimation of research and development funding for improving economic feasibility. Carbon offset of the proposed technology has been estimated and carbon credit results have been incorporated into the economic feasibility metrics.Publication Metadata only Valorization of pea pod, celery root peel, and mixed-vegetable peel as a feedstock for biocellulose production from Komagataeibacter hansenii DSM 5602(SPRINGER HEIDELBERG) KAZAN, DİLEK; Bozdag, Gulnihal; Pinar, Orkun; Gunduz, Oguzhan; Kazan, DilekCurrently, recycling and reuse of wastes to obtain high value-added products are substantial issues for development of sustainable and economic processes. Among these wastes, evaluation of food waste has been received significant attention due to scarcity in undeveloped countries, food security, and environmental problems. In general, this study focused on the investigation of cheap carbon sources and re-utilization of food waste for the production of bacterial cellulose (BC). Therefore, pea pod, celery root peel, and mixed-vegetable peel were evaluated to produce BC from Komagataeibacter hansenii (waste-based Kh-BC) in the present work. Subsequent to the BC production from specified wastes, chemical structure, thermal properties, scanning electron microscope (SEM) analysis, water uptake, and antibacterial activity of BC were analyzed. Among all wastes studied, mixed-vegetable peel and pea pod were positive influencers on BC synthesis and Fourier-transform infrared (FT-IR) spectra of BC membranes produced from wastes were very similar to that obtained from mannitol as a control. Additionally, waste-based Kh-BC has higher biodegradability and thermal stability than the Kh-BC produced from the control medium. Although it has a fragile structure, its water holding capacity and porous structure appear similar to standard BC. Moreover, waste-based Kh-BC could be impregnated with antibiotics to obtain the antibacterial BC membrane. Therefore, the present work showed that vegetable wastes could be valorized for BC production and waste-based Kh-BC is a promising biopolymer candidate for medical and pharmaceutical applications according to its properties.Publication Open Access A two-step purification platform for efficient removal of Fab-related impurities: A case study for Ranibizumab(2023-11-01) PİNAR, ORKUN; SARIYAR AKBULUT, BERNA; KAZAN, DİLEK; Tatli O., Oz Y., Dingiloglu B., Yalcinkaya D., Basturk E., Korkmaz M., Akbulut L., Hatipoglu D., Kirmacoglu C., Akgun B., et al.Antibodies (mAbs) and antibody fragments (Fabs) constitute one of the largest and most rapidly expanding groups of protein pharmaceuticals. In particular, antibody fragments have certain advantages over mAbs in some therapeutic settings. However, due to their greater chemical diversity, they are more challenging to purify for large-scale production using a standard purification platform. Besides, the removal of Fab-related byproducts poses a difficult purification challenge. Alternative Fab purification platforms could expedite their commercialization and reduce the cost and time invested. Accordingly, we employed a strong cation exchanger using a pH-based, highly linear gradient elution mode following Protein L affinity purification and developed a robust two-step purification platform for an antibody fragment. The optimized pH gradient elution conditions were determined on the basis of purity level, yield, and the abundance of Fab-related impurities, particularly free light chain. The purified Fab molecule Ranibizumab possessed a high degree of similarity to its originator Lucentis. The developed purification platform highly intensified the process and provided successful clearance of formulated Fab- and process-related impurities (∼98 %) with an overall process recovery of 50 % and, thus, might be a new option for Fab purification for both academic and industrial purposes.Publication Metadata only Enzyme mixture production from Pycnoporus sanguineus DMSZ 3024 using a lignocellulosic waste, Hazelnut husk: A case study for laccase and cellulase(ELSEVIER SCIENCE BV, 2014) KAZAN, DİLEK; Pinar, Orkun; Kochan, Basak; Sayar, Nihat Alpagu; Karaos-Manoglu, Kubra; Kazan, DilekPublication Open Access A preliminary study on purification and characterization of lipase(s) produced by cryptococcus diffluens D44(2023-03-01) PİNAR, ORKUN; Büyük E., Pinar O.In the present work, preliminary purification, and characterization of lipases from Cryptococcus diffluens D44, which was isolated from petroleum sludge, were performed. In the purification steps, subsequential to acetone precipitation, lipases from C. diffluens D44 were purified by DEAE Sepharose resulting in two different peaks, named Lip1 and Lip4. Sephadex G-100 size-exclusion chromatography was also performed for further purification of Lip1 and Lip4 and resulted in three different lipases as Lip1-1 (1.0 purification fold with 2.4% recovery), Lip1-2 (0.8 purification fold with 7.2% recovery), and Lip4-1 (1.2 purification fold with 4.5% recovery). As a result of characterization studies of these three lipases resulting from different peaks, optimum temperatures were found as 60 °C, 65 °C, and 65 °C for Lip1-1, Lip1-2, and Lip4-1, respectively. Furthermore, thermal stability studies were conducted at 50 °C, 60 °C, and 70 °C, and lipases of C. diffluens D44 maintained over 70% of their initial activity at 50 °C. The optimum pH for Lip1-1 and Lip1-2 was pH 9.0 although pH 5.0 was for Lip4-1. Considering the organic solvent effect on lipase activity, 10% methanol enhanced the relative activity of Lip1-1 and Lip4-1 while 10% ethanol caused a decrease in the relative activity of lipases except for Lip1-2. According to the indicated features based on the results, these different lipases from C. diffluens D44 could be promising candidates for industrial and biotechnological applications. To the best of our knowledge, this is the first study on the purification of lipases from C. diffluens D44Publication Metadata only Production of industrial enzymes for feed industry(ELSEVIER SCIENCE BV, 2012) SARIYAR AKBULUT, BERNA; Sogut, Melis S.; Ozturk, Nurcin C.; Ozturk, H. Umit; Pinar, Orkun; Ipek, Fatih M.; Cayir, Ebru; Gokce, Abdulmecit; Karasu, Deniz; Ozturk, Dilek C.; Ozturk, Yavuz; Denizci, A. Akin; Ozturk, Bengu; Bahar, Banu; Avci, Naze G.; Ayan, Burak; Kazan, Dilek; Duman, Yonca A.; Sargin, Sayit; Karacanli, Sevket; Mizrak, Cengizhan; Catli, A. Ugur; Kucukyilmaz, Kamil; Guvenmez, Hatice K.; Arikan, Burhan; Bozkurt, Mehmet; Seyhan, Ferda; Akbulut, Berna S.; Erarslan, A. Altan; Ongen, Gaye; Dincer, Sadik; Yenice, Engin; Mandaci, SevnurPublication Metadata only Techno-economic analysis of caffeine and catechins production from black tea waste(ELSEVIER, 2019) SARIYAR AKBULUT, BERNA; Sayar, Nihat Alpagu; Sam, Selcen Durmaz; Pinar, Orkun; Serper, Damla; Akbulut, Berna Sanyar; Kazan, Dilek; Sayar, Ahmet Alpy This work establishes a proof of concept level economic assessment of a novel commercial production scheme for caffeine and catechins from black tea waste collected in the north-eastern region of Turkey. A base case in which dichloromethane (DCM) is used as the product extracting agent and an alternative greener case where DCM is replaced with environmentally friendlier ethyl acetate (EA) were designed and modelled on Superpro Designer platform. Profitability analyses were conducted for both cases, each treating 30,000 MT/year of black tea waste. Under the base case scenario, the Net Present Value (NPV) was calculated as 16,249,000 USD whereas the alternative scenario gave an NPV of 4,379,000 USD with corresponding payback times of 5.64 and 6.79 years. A novel techno-economic evaluation method involving the metamodeling of Superpro Designer models via Kriging and subsequent multi-objective optimization is incorporated into the economic assessment and applied to the two cases in order to optimize selected dominant process variables. Optimal values for dominant operating process variables were found using 3-D graphs simulating the combined effects of these variables on NPV. Results showed that the base case has a greater potentiality to generate profit. However, the greener case could be implemented if it is supported on environmental and toxicity issues considering the potential health and pollution preventive benefits. The present work demonstrates how the integration of novel conceptual design, modelling, and optimization approaches can foster broader evaluation of bioprocesses utilizing negative-value agro-wastes for the production of value-added chemicals. (C) 2019 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.Publication Open Access Determination of the Significance of the Most Effective Nutrients on Lipase Production from Cryptococcus albidus D24(2022-06-01) PİNAR, ORKUN; KAZAN, DİLEK; Uras, Abdussamed; Uras A., Pinar O., Kazan D.In this study, the optimization of the medium components used for the production of lipase enzyme from Cryptococcus albidus D24 was performed using the Plackett-Burman statistical design method (PBD), and the most important nutrients affecting the production of lipase enzyme from D24 strain were determined as the first step. According to PBD, the highest lipase activity (19.34 U/ml/min) was obtained with medium including Tween 80 (X2) 2.5% (v/v), and (g/L) Peptone (X4) 8.0, Yeast Extract (X6) 7.5, Beef Extract (X7) 7.5, Malt Extract (X8) 7.5, NH4Cl (X9) 6.0, NaNO3 (X10) 1.5, (NH4)NO3 (X12) 6.0, (NH4)HCO3(X13) 6.0, MgSO4.7H2O (X15) 1.0, and KH2PO4 (X16) 2.0 at the end of 144 h cultivation. Regarding the concentration effect (CE) values obtained from PBD, NH4Cl (CE=7.1587), olive oil (CE=3.5544), (NH4)HCO3 (CE=3.0747), and tryptone (CE=2.1427) were evaluated as the more effective nutrients among the sixteen compounds studied. After that, the optimum concentrations of these effective compounds were experimented with Response Surface Methodology (RSM). Experimental results showed that the medium containing olive oil (X3) 1.5% (v/v), and (g/L) tryptone (X5) 3.0, NH4Cl (X9) 7.5, and (NH4)HCO3 (X13) yielded maximum lipase activity (12.03 U/ml/min) compared to other studied compounds. Although the maximum lipase activity obtained with RSM methodology was lower than that obtained by PBD, the cost of the nutrients used to produce one-unit enzyme is 0.104 Euro in the PBD, while only 0.0277 Euro is spent in RSM. In other words, the production of lipase using compounds coded X3, X5, X9, and X13 provides a cost-effective process.Publication Metadata only Assessment of poly(3-hydroxybutyrate) synthesis from a novel obligate alkaliphilic Bacillus marmarensis and generation of its composite scaffold via electrospinning(ELSEVIER SCIENCE BV, 2018) KAZAN, DİLEK; Ozgoren, Tugba; Pinar, Orkun; Bozdag, Gulnihal; Denizci, Aziz Akin; Gunduz, Oguzhan; Hatir, Pinar Cakir; Kazan, DilekIn this study, poly(3-hydroxybutyrate) (PHB) production from a newly isolated obligate alkaliphilic Bacillus marmarensis DSM 21297 was investigated to evaluate the ability of obligate alkaliphilic strain to produce a biopolymer. Additionally, electrospun nanofibers from B. marmarensis PHB (Bm-PHB) were generated using Bm-PHB/polycaprolactone (PCL) blend to evaluate the applicability of Bm-PHB. According to the experimental results, the metabolic activity of B. marmarensis decreased the pH of the medium by generating H+ ions to initiate Bm-PHB production, which was achieved at pH below 9.0. Regarding medium components, the addition of MgSO4 center dot 7H(2)O and KH2PO4 to the medium containing 1% glucose enhanced the amount of Bm-PHB synthesis, and an approximately 60% increase in PHB concentration was obtained in the presence of mineral salts. Based on FTIR analysis, the chemical structures of Bm-PHB and commercial PHB were found to be highly similar. Additionally, the T-g and T-m values of Bm-PHB were determined to be 17.77 degrees C and 165.17 degrees C, respectively. Moreover, Bm-PHB/PCL composite scaffold was generated by electrospinning method that produced nanofibers between 150 and 400 nm in diameter, with an average of 250 nm. To our knowledge, this is the first report to produce PHB from an obligate alkaliphilic Bacillus strain and PHB scaffold. (C) 2018 Elsevier B.V. All rights reserved.Publication Metadata only Bioethanol Production From Turkish Hazelnut Husk Process Design and Economic Evaluation(SPRINGER, 2019) SAYAR, AHMET ALP; Sayar, Nihat Alpagu; Pinar, Orkun; Kazan, Dilek; Sayar, Ahmet AlpThe main objective of the current study is to develop and assess the preliminary synthetic design steps of an innovative and unprecedented bioprocess plant converting Turkish hazelnut husk into lignocellulosic ethanol with an emphasis on economic evaluation. Valorization of this agricultural waste would provide a promising economic potential and long-term sustainability with acceptable environmental impact. Preliminary economic evaluations are performed on several scenarios where the effects of changing various process design and operational inputs such as designed plant capacity, evaporation unit operation efficiency, and biocatalyst and nitrogen source prices are simulated. The total capital investment for the base case scenario with an annual throughput of 180,000 metric tons (MT) hazelnut husk is just above USD 111million. The annual operational costs for this case amount to USD 61million. Assuming a sales price of USD 1.50 per kg of bioethanol (achieved via governmental subsidy and tax incentives) the return on investment of the project comes to 12.61% with a 8year payback period. An uncertainty analysis performed on the annual hazelnut husk availability and biocatalyst and nitrogen source price fluctuations establishes a basis for further design of the process taking into account the risk factors associated with the project. The case studies and the uncertainty analysis confirm the fact that production of second generation bioethanol from hazelnut husk in Turkey is a worthwhile endeavor with an economic potential especially with additional social and environmental advantages.