Person:
ŞAHBAZ, SEVİNÇ

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

ŞAHBAZ

First Name

SEVİNÇ

Name

Filters

2015 - 201912020 - 20222
Reset filters

Settings

Has files: true ×

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    PublicationOpen Access
    Synthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute
    (2022-08-01) SEZER, ALİ DEMİR; UĞURLU, TİMUÇİN; ŞAHBAZ, SEVİNÇ; Doğan M., Şahbaz S., Uğurlu T., Sezer A. D.
    The aim of the current study was to assess the physicochemical characteristics and wound healing activity of chitosan-polyvinyl alcohol (PVA) crosslinked hydrogel containing recombinant human epidermal growth factor (rh-EGF) or recombinant mouse epidermal growth factor (rm-EGF). The hydrogels were prepared and analyses were made of the morphological properties, viscosity, water absorption capacity, mechanical and bio-adhesive properties. The viscosity of the formulations varied between 14.400 - 48.500 cPs, with the greatest viscosity values determined in K2 formulation. F2 formulation showed the highest water absorption capacity. According to the studies of the mechanical properties, H2 formulation (0.153±0.018 N.mm) showed the greatest adhesiveness and E2 (0.245±0.001 mj/cm2 ) formulation, the highest bio-adhesion values. Hydrogels were cytocompatible considering in vitro cell viability values of over 76% on human keratinocyte cells (HaCaT, CVCL-0038) and of over 84% on human fibroblast cells (NIH 3T3, CRL-1658) used as a model cell line. According to the BrdU cell proliferation results, B1 (197.82±2.48%) formulation showed the greatest NIH 3T3 and C1 (167.43±5.89%) formulation exhibited the highest HaCaT cell proliferation ability. In addition, the scratch closure assay was performed to assess the wound healing efficiency of formulation and the results obtained in the study showed that F2 formulation including PEGylated rh-EGF had a highly effective role. Keywords: Chitosan. rh-EGF. Hydrogel. Texture analyzer. Cell culture.
  • Thumbnail Image
    PublicationOpen Access
    Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-beta 1 and evaluation of in vitro wound healing effect
    (MARMARA UNIV, 2020-03-12) SEZER, ALİ DEMİR; Soysal, Aysun Celik; Sahbaz, Sevinc; Ugurlu, Timucin; Sezer, Ali Demir
    ( )Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factor stimulates (TGF-beta) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradation produces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine the therapeutic effect of the TGF-beta 1with the positive effect of the drug delivery system including PLGA nanoparticles (TGF beta-PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitory effect of TGF-beta 1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferative effect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-beta 1 has an inhibitory effect on keratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay.
  • Thumbnail Image
    PublicationOpen Access
    Evaluation of Pharmacy Manager's Knowledge and Attitude on Extemporaneous Preparation
    (MARMARA UNIV, FAC PHARMACY, 2015-08-06) OKUYAN, BETÜL; Andac, Ayca; Yasayan, Gokcen; Alarcin, Emine; Okuyan, Betul; Sahbaz, Sevinc
    In this work, it is aimed to determine pharmacists' attitude, level of knowledge on extemporaneous formulations including how accurate the formulations are prepared, the problems pharmacists face during preparation, and their approach to solve problems on extemporaneous preparation. Additionally with this work, we aim to gather data on frequently written extemporaneous prescriptions during the recent years. In this work, a questionnaire with the title of Evaluation of pharmacy manager's knowledge and attitude on extemporaneous preparation was applied to pharmacy managers. This questionnaire was completed by 54 community pharmacists. According to our results, it was found that extemporaneous preparations were prescribed to all of the pharmacies that the questionnaire applied, however pharmacists were confronted with various problems during preparation of the formulations.